Objective To discuss the influence of blood exchange transfusions on hemato- microenvironment of neonates with hyperbilirubinemian.Methods Blood exchange transfusion was performed in 35 neonates.Mean transfusion volume was (136.9±27.4) ml/kg.The speed of blood exchange transfusion was 92 ml/(kg·h).Blood bilirubin,blood electrolyte,blood routine and blood sugar levels were measured before,during and after the transfusion.Results Serum total bilirubin (TB) decreased by 53.78% after blood exchange,but there was no change in Na~+,Cl~-,and blood sugar (P＞0.05),and K~+ was remarkably decreased from (4.45±2.40)mmol/L to (3.87±0.52)mmol/L (t=21.979,P＜0.05).TB,Hb,PLT,WBC,Ca~(2+) were decreased[TB from(511.52±80.21) μmol/L to (236.41±66.54) μmol/L;Hb from (145.20±11.70) g/L to (128.66±2.54) g/L;PLT from (207.84±70.67)×10~9/L to (134.86±48.61)×10~9/L;WBC from (16.90±10.56)×10~9/L to (8.94±7.44)×10~9/L,and Ca~(2+) from (1.26±0.32) mmol/L to (0.99±0.22) mmol/L,P＜0.05 or ＜0.01].Conclusions Blood exchange transfusion can not influence Na~+,Cl~-,and blood sugar,but has remarkable effect on total bilirubin,Ca~(2+),Hb,K~+,PLT,and WBC.

Objective To analyze the association ofdopamine receptor D2 (DRD2) TaqIA gene polymorphism with persistent postural-perceptual dizziness (PPPD) and explore the molecular mechanism of this disease.Methods The study group consisted of 43 patients diagnosed as having PPPD was chosen in our hospital from January 2017 to June 2017;45 gender-and age-matched control subjects were selected randomly from acute vertigo patients who were fully recovered within 3 months without selective serotonin reuptake inhibitors (SSRIs) or serotonin norepinephrine reuptake inhibitors (SNRIs) medication and were normal at 6 months of follow up.Personality characteristics of the two groups were analyzed by adult Essen personality inventory.Frequencies of DRD2 TaqIA gene polymorphism were detected with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).Results Percentage of neurotic individuals in study group (67.4％) was significant higher than that of control subjects (37.8％,P＜0.05).Genotype distribution ofA1/A1,A1/A2,and A2/A2 showed significant difference between the two groups (P＜0.05).In the study group,A1 and A2 allele frequencies in the DRD2 TaqIA gene were 65.1％ and 34.9％ respectively,which had significantly difference as compared with those of control subjects (46.7％ and 53.3％,P＜0.05).Conclusion A 1 allele in DRD2 TaqIA gene may be the susceptibility gene for PPPD.

Objective:To analyze the relation between DNA methylation in the CpG island of dopamine D2 receptor ( DRD2) gene promoter region and persistent postural-perceptual dizziness (PPPD), and explore the molecular mechanism of PPPD. Methods:The disease group consisted of 43 patients diagnosed as having PPPD in our hospital from January 2017 to June 2017, and blood samples were taken at admission. The control group included 45 with acute vestibular peripheral vertigo whose dizziness symptoms did not recrudesce after follow-up for more than 3 months and PPPD diagnosis was excluded in our hospital at the same period; these patients did not take selective serotonin reuptake inhibitors (SSRIs) or serotonin norepinephrine reuptake inhibitors (SNRIs); blood samples in the patients were collected during follow-up. DNA methylation in the CpG island of DRD2 promoter region was detected by disulfite sequencing and the differences between the two groups were compared. Results:The positive rate of DNA methylation in the CpG island of DRD2 promoter region in the disease group was 58.1%, which was significantly higher than that in the control group (15.6%, P<0.05); and the methylation rate of CpG island loci in the disease group (0.15±0.18) was significantly higher than that in the control group (0.04±0.10, P<0.05). Conclusion:The DNA methylation in the CpG island of DRD2 promoter region is associated with onset of PPPD.

Electrospray technology is a method to prepare microspheres by electrostatic force. Electrospray technology has the advantages of simple operation and mild reaction conditions. The polymer microspheres prepared by electrospray technology have uniform morphology and good monodispersity, which is a new and promising method. In this paper, the devices and principles of electrospray technology, the factors affecting the morphology and particle size of the prepared polymer microspheres by the electrospray process, and the types of polymer solutions commonly used in electrospray technology were described, and the applications of electrospray technology for drug delivery, loaded nanoparticles, cell therapy, and bioactive substance delivery were reviewed. It can be concluded that electrospray technology has a broad application prospect and potential application value.