0.05 ;Ti milled/VITA vs Ni Cr/VITA P

Objective:To study the feasibility of microarc oxide treatment on the enhancement of titanium-ceramic bonding strength. Method:Titanium samples in the size of 25 mm?3 mm?0.5 mm were prepared with smooth surface(group 1),rough surface(group 2) and microacrc oxide treated surface(group 3). Nickel-chromium alloy samples in the same size were prepared (group 4). The surface of the samples was observed by scanning electron microscope(SEM) and dispersive spectrometry analysis. Then, the samples were bonded to porcelain. The bonding interface was observed by SEM. The bonding strength of the samples was measured by a three-point bending test according to ISO 9693.Results:Microarc oxide treated surface was rough and porous. The interface of microarc oxide treated surface bonded to porcelain was compact. The bonding strength(MPa) of the samples of group 1,2,3 and 4 to porcelain was 30.79?1.3,36.12?3.03,45.84?3.15 and 48.35?3.06 respectively(group 3 vs group 1 or 2 P0.05). Conclusion:The microarc oxide treatment on titanium can increase the titanium-ceramic bond strength.

Objective:To evaluate the effect of preoxidation on the bonding strength of titanium to porcelain.Method:12 titanium plates(25 mm?3 mm?0.5 mm) were pre-oxidated according to usual preoxidation procedure and 12 without preoxidation were used as the controls.The samples(6 in each group) were then bonded with bonding porcelain,opaque porcelain,dentin porcelain and glaze of Duceratin(Degussa) or Vita titankeramik(Vita) respectively.The bonding strength of Ti/porcelain was evaluated using three-point bending test according to ISO 9693 1990 standard. The interface of Ti/porcelain bonding was observed by SEM.Results:Bonding strength(MPa) of Ti/Duceratin preoxided group and the corresponding control was 41.910?2.778 and 33.097?5.297(P0.05),respectively.SEM observation showed inter-lock and tight bonding of Ti/porcelain in pre-oxidated interface,more cracks and gaps between Ti and porcelain in non-pre-oxidated interface.Conclusion:Pre-oxidation may improve bonding strength of Ti/Duceratin system, while has no significant effect on bonding strength of Ti/Vita titankeramik system.

Objective To investigate the hepatoprotective effects of total flavonoids in Scorzonera austriaca Wild (TFSA) in vivo and in vitro. Methods In vivo, ICR mice were randomly divided into negative, model, positive, TFSA’s low-dose, medium-dose and high-dose groups,and acute chemical liver injury models were constructed with CCl4 and acute autoimmune liver injury models with Bacillus Calmette-Guerin vaccine ( BCG ) and lipopolysaccharide (LPS).The activity of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) was detected and liver tissue was used as biopsy.In vitro, liver cells of Wistar rat were extracted and isolated by orthotopic collagenase digestion method, and liver cell damage was induced with CCl4.Then the liver cells were cultured with TFSA solution and the contents of AST, ALT, LDH, nitric oxide (NO), superoxide dismutase (SOD) in the supernatant and malondialdehyde (MDA) in rat hepatocyte were detected.Results The results of CCl4-and BCG+LPS-induced acute chemical liver injury models in mice showed that there were less microstructures damage of liver tissue in TFSA groups compared with model group in liver pathological sections (HE), AST, ALT and LDH levels in model group were significantly higher than those in negative group (P<0.01), the above indexes in positive drug group were significantly lower than those in negative group (P<0.01,P<0.05), and the above indexes in TFSA’s low-dose, medium-dose and high-dose groups were significantly lower than those in model group(P<0.01,P<0.05).The results of CCl4-induced rat hepatocyte injury in vitro showed that AST, ALT, LDH, MDA and NO levels were significantly higher and SOD level was lower in model group than those in negative group (P<0.01), AST, ALT, LDH, MDA and NO levels were significantly lower and SOD level was higher in positive drug group than those in model group (P<0.01, P<0.05), and AST, ALT, LDH, MDA and NO levels were significantly lower and SOD level was higher in TFSA ’ s low-dose, medium-dose and high-dose groups were significantly lower than those in model group ( P <0.01, P <0.05 ) .Conclusion TFSA have hepatoprotective effects on CCl4-induced chemical liver injury and BCG+LPS-induced immune liver injury in mice, and rat hepatocyte damage.This study provides experimental data for the development and utilization of Scorzonera austriaca Wild resources and new hepatoprotective medicines.

Objective To investigate perioperative nursing of respiratory system in severe scoliosis patients.Methods 26 severe scoliosis patients with respiratory function training before operation were retrospected.All of them tested pulmonary function before and after training,postoperative respiratory system nursing were also taken to maintain airway unobstructed,including respiratory rate and oxygen saturation monitoring,effective pain management and respiratory complications controlling.Results The average forced vital capacity(FVC)was 45.9%and 52.1%before and after breathing exercises,the mean forced expiratory volume in one second(FEV1)was 43.8%and 48.8%before and after breathing exercises,with lung function improvement in 69.2%patients. There were two hemopneumothorax and three mild or moderate pleural effusion occurred within 10 days after surgery,but all recovered after effective treatment and nursing.Conclusions Perioperative systematic and effective training in respiratory function and airway management can improve lung function and surgical safety,reduce the incidence of postoperative pulmonary complications,and promote early rehabilitation .

Objective To investigate the effects of DanzhiⅠ on rats acute skin trauma model.Method Perforate back of the rats was made with special puncher to establish acute trauma model.Wound surface was applied with Danzhi Ⅰ and the healing process was observed.Result Danzhi Ⅰ significantly shrinked the rim of raw surface and shorten the time of healing.The number of completely healed rats increased obviously.Conclusion Danzhi Ⅰ can significantly accelerate the healing of acute skin trauma of rats.

Objective This study is to identify long-term functional recovery and maturity of regenerated nerve fibers after repairing mouse nerve defects with chitosan/polylactide-co-polyglycolide artificial nerve grafts ( CPANGs ) . Methods Mouse sciatic nerve defects, 2mm in length, were bridged by CPANGs (n=6), with nerve autograft (n=6) and nerve defect (n=6) as controls.Plantar test, electrophysiological examination and laser Doppler perfusion imaging following nerve crush were carried out at 1 year after repair to assess nerve function recovery , while muscle wet weight ratio, histological assessment and transmission electron microscopy were performed to evaluate nerve re -innervation and maturity of regenerated nerve fibers .Results When compared to the autograft group , the CPANG group did not show statistically significant difference in functional recovery in terms of paw withdrawal latency , neurogenic vasodilatation , amplitude and latency of compound muscle action potentials ( CMAPs ) , wet weight ratio of gastrocnemius and tibialis cranialis muscles , number of myelinated nerve fibers and density of unmyelinated axons .However , both these two repair groups exhibited significantly longer CMAPs latency , thinner myelin sheath and a lag-behind shift of diameter distribution of myelinated axons as compared to the normal control .Conclusion At 1 year after the mouse sciatic nerve defect was repaired by CPANGs , sensory and autonomic nerve function , number of regenerated axons and muscle re-innervation degree were recovered to the same extent as nerve autografting , but the regenerated nerve fibers were in a state of immaturity .

Objective To investigate impaired fasting glucose(IFG)status of office worker and enterprise personnel in Shiji-azhuang area,for providing a basis for early prevention of diabetes mellitus.Methods The fasting blood glucose level of 109 083 of-fice workers and enterprise personnel in Shijiazhuang area were detected in physical examination in 2012.Results The people of IFG (fasting blood glucose 5.6-<7.0 mmol/L)accounted for 14.61% in all subjects,1 6.61% in men,10.23% in women.3.5 1%male of the 18-<21 years old group were IFG.With the increasing of age,the ratio of IFG people gradually rised to a peak of 30.44% at the age group of 71-<76,and then decreased a little.While the proportion of female with IFG was 1.55% at 18-<21 years old group,with the increasing of age,to the age group of 5 1 - < 56 reached the first peak 20.27%,then decreased at the 56-<61 age group.Rapidly rising again,up to 31.74% the second peak in 76-<81 age group,and then decreased a little.Conclu-sion The proportion of IFG in male is higher than that in female.The proportion of IFG increases with age,and reaches the peak in a certain age (over 70 years old),and then at a relatively stable level.

Aim To explore the possible protective effect of ginsenoside Rg1 on oligomeric Aβ1-42 induced apoptosis and its possible mechanism. Methods The damage was induced by oligomeric Aβ1-42 in primary cortical neurons. Cells were incubated in the absence or presence of Aβ, or co-incubated in sp600125 with Aβ, or pre-incubated in ginsenoside Rg1 then co-incu-bated in Aβ. The p-JNK, JNK, caspase-3 activity and TUNEL-positive cells were detected. Results In Aβ1-42 treated group, the ratio of p-JNK/JNK level was increased more than that in non-treated group for 15 min. However, in neurons preincubated with (2. 5, 5, 10 μmol·L-1 ) ginsenoside Rg1 and then co-incuba-ted with 5 μmol·L-1 oligomeric Aβ1-42 , the p-JNK/JNK ratio, caspase-3 activity and TUNEL positive neu-rons were significantly decreased compared with those of Aβ1-42 treated group. Conclusion Ginsenoside Rg1 can attenuate the oligomeric Aβ1-42-induced apop-tosis by JNK pathway.

ObjectiveTo explore the risk factors for the prognosis of respiratory failure in neonates with gestational age≥34 weeks. MethodsA total of 143 hospitalized neonates with respiratory failure who had gestational age≥34 weeks were enrolled from Jan. 2011 to Jun. 2013. According to the outcome, the neonates were divided into good prognosis group and poor prognosis group. The risk factors for the prognosis of respiratory failure were screened by univariate analysis and mul-tivariate binary logistic regression model.ResultsAmong the 143 neonates, 105 neonates had good prognosis and 38 neonates had poor prognosis. Univariate analysis showed that the mode of delivery, small for gestational age, 5 minutes Apgar score <7, and with primary disease being pneumonia/sepsis were associated with a poor prognosis. The differences were statistically signif-icant (P<0.05). Furthermore, the multivariate binary logistic regression model showed that, small for gestation age (OR=23.87, 95%CI:3.149-180.9) and pneumonia/sepsis (OR=2.996, 95%CI:1.514-5.928) were the independent factors of poor prognosis. ConclusionsSmall for gestation age and primary diseases being pneumonia/sepsis are the independent factors inlfuencing prog-nosis of respiratory failure in neonates born at a gestational age≥34 weeks.