The degranulation of mast cells represents a pivotal e-vent in the allergic disorders.The Src family kinases(SFKs)are as a starting signal in the activation of mast cell.Lyn,Fyn and Syk play important regulatory role in the degranulation of mast cells.Regulating SFKs can reduce the degranlation process and inhibit the allergic disorders.Therefore,SFKs inhibitors can be potential drugs in the allergy.It is necessary to study the targeted medicine of SFKs,which will be a new direction of drug develop-ment.

Objective To evaluate the effect of intensity-modulated radiation therapy(IMRT) on parotid function in nasopharyngeal carcinoma(NPC). Methods Eighty-three NPC patients received prima-ry IMRT between 2001 and 2003. Xerostomia before radiotherapy, at the end of radiotherapy, at 6-month, 1-,2-,3-,4- and 5-year after radiotherapy were investigated, respectively. The relation between xerostomia and parotid dose distribution was analyzed. Results Of all the patients,4,31,31 and 17 had stage Ⅰ,Ⅱ,Ⅲ and ⅣA disease, respectively. Sixteen patients received chemo-radiotherapy. The median followed-up time was 65 months. The 5-year local control and regional control rate were 96% and 95% ,respectively. The 5-year overall survival rate was 80%. The mild xerostomia rate at the seven time points was 42%, 51%, 71%, 77%, 58%, 38% and 26%. The corresponding moderate xerostomia rate was 52%, 53%, 21%,8%, 3%, 2% and 2%, respectively. No serious xerostomia was observed. The mean dose of the bilateral parotid glands was 34.34 Gy. Xerostomia at 6-month after radiotherapy was positively correlated with the mean dose of the parotid glands, and D50 was the independent factor in predicting the xerostomia. Parotid function was well protected when the mean dose and D50 were no more than 33 Gy and 29 Gy,respectively. Conclusions IMRT can improve the local-regional control of NPC and protect the parotid glands from radiation-induced in-jury.

This paper introduces an algorithm for detecting T wave alternans (TWA) using Poincare mapping method, which is a technique for nonlinear dynamic systems to display periodic behavior. Vector angle index (VAI) was used to determine the presence or absence of TWA. Adopting MIT/BIH Arrhythmia database and European ECG ST-T database to simulate, we used VAI via poincare mapping method for correlation analysis with Vtwa by way of the spectral method. The cross-correlation coefficient between Vtwa and VAI is gamma = 0.860 1. The algorithm can identify the absence and presence of TWA accurately and provide idea for further study of TWA.
Algorithms ; Arrhythmias, Cardiac ; physiopathology ; Computer Simulation ; Electrocardiography ; methods ; Humans ; Signal Processing, Computer-Assisted ; Spectrum Analysis ; methods

Objective To investigate the targeting effect of TLS9a nucleic acid aptamer on mice hepatic cancer cells.Methods The liposome modified with maleimide and loading doxorubicin(DOX) was prepared,then TLS9a nucleic acid aptamer modified by FITC fluorescence and sulfydryl was synthesized,which was coupled to the liposome surface.The entrapment efficiency of DOX was detected by UV spectrophotometry.The dynamic light scattering(DLS) was applied to measure the particle size of nanoparticles and the potential distribution.The uptake of DOX in mice hepatic cancer cells was detected by the Nikon inverted microscope and the mean fluorescence intensity of liposome/DOX and TLS9a-liposome/DOX was detected by flow cytometry.The cells activity was detected by MTT.Results Flow cytometry assay showed that the binding rate of TLS9a nucleic acid aptamer with BNL.1ME.A.7R.1 mice hepatic cancer cells was 54.1％.TLS9a-liposome particle size distribution was in (116.0 ± 5.0)nm.TLS9a-liposome/DOX released DOX quickly at pH 5.0,and the release amount in 72 h was more than 70 ％ of the total release amount.TLS9a-liposome/DOX effectively inhibited the growth of mice hepatic cancer cells BNL.1ME.A.7R.1.Conclusion TLS9a nucleic acid aptamer could specifically combined with mice hepatic cancer cells BNL.1ME.A.7R.1,which could be used to detect mice hepatic cancer cells.

To study the distribution pattern of drug dust in the antibiotic raw material drug powder screening workshop and improve the working environment, we used COMSOL Multiphysics 6.0 software to simulate and study the airflow distribution and dust transport law in the powder screening workshop. The results indicated that the dust in the powder screening workshop diffused rapidly with the airflow and reached a stable state at the 100th second. After the dust migrated to a stable state, the area with excessive dust concentration (dust concentration>6 mg/m 3) widely distributed, mainly distributed in the middle and lower parts of the wall opposite the air inlet from the rotary vibrating screen. The distribution of dust showed a characteristic of local aggregation, and dust transport was related to airflow movement, which was prone to aggregation in areas with low airflow velocity and eddy currents. On the plane of the human respiratory belt height (h=1.5 m), the concentration near the dust producing surface was high, with local concentrations exceeding 32 mg/m 3. It was the key area for dust prevention and control. And the working environment can be improved by changing the air flow distribution in the workshop or other dust reduction methods.

To study the distribution pattern of drug dust in the antibiotic raw material drug powder screening workshop and improve the working environment, we used COMSOL Multiphysics 6.0 software to simulate and study the airflow distribution and dust transport law in the powder screening workshop. The results indicated that the dust in the powder screening workshop diffused rapidly with the airflow and reached a stable state at the 100th second. After the dust migrated to a stable state, the area with excessive dust concentration (dust concentration>6 mg/m 3) widely distributed, mainly distributed in the middle and lower parts of the wall opposite the air inlet from the rotary vibrating screen. The distribution of dust showed a characteristic of local aggregation, and dust transport was related to airflow movement, which was prone to aggregation in areas with low airflow velocity and eddy currents. On the plane of the human respiratory belt height (h=1.5 m), the concentration near the dust producing surface was high, with local concentrations exceeding 32 mg/m 3. It was the key area for dust prevention and control. And the working environment can be improved by changing the air flow distribution in the workshop or other dust reduction methods.

Cardiovascular disease presents a serious threat to human life,and oxidative stress has been identified as an important factor affecting the occurrence and development of cardiovascular disease.The construction of reliable animal models of oxidative stress is important for the in-depth study of the pathogenesis of cardiovascular diseases and the development of therapeutic drugs.Zebrafish have often been for research into cardiovascular diseases,due to their advantages of easy reproduction,a short developmental cycle,transparent embryos for easy observation,and a highly homologous cardiovascular genetic background with humans.In this paper,we review the application of the zebrafish oxidative stress model in cardiovascular disease and related research progress,to provide a reference for its further application in cardiovascular disease-related research.

Objective To analyze the clinical value of digital breast tomosynthesis (DBT) compared with digital mammography (DM) and ultrasound for diagnosing non-calcified masses in dense breasts.Methods Images taken with DBT,DM and ultrasound of 1144 patients with non-calcified masses in dense breasts were retrospectively analyzed using breast imaging reporting and data system (BI-RADS).Taking histopathologic results as golden standards,the detection rate and diagnostic accuracy,sensitivity,specificity,false negative and BI-RADS category were evaluated and compared statistically.Results The detection rate of DBT,DM and ultrasound for non-calcified massed in dense breasts was 86.62％ (991/1 144),77.80％ (890/1 144) and 99.65％ (1 140/1 144),respectively (P＜0.05),while the diagnostic accuracy was 83.92％ (960/1 144),75.00％ (858/1 144) and 94.67％ (1 083/1 144),respectively (P＜0.01).The sensitivity of DBT,DM and ultrasound was 89.39％ (312/349),79.93％ (231/289) and 92.70％ (432/466),the specificity was 81.51％ (648/795),73.33％ (627/855) and 96.02％ (651/678),while the false negative rate was 10.60％(37/349),20.07％ (58/289) and 7.30％ (34/466),respectively.No significant difference was found for benign lesions among three examination methods (P=0.75),while there was significant difference for malignant lesions among three examination methods (P＜0.01),and the differences of ultrasonography with DM and DBT,DBT and DM in the for BI-RADS category of malignant lesions were statistically significant (all P＜0.016 7).Conclusion For suspected masses in dense breasts,DBT shows significant advantage than DM,while DBT has the similar advantage compared with ultrasound for the detection and diagnosis of non-calcified masses in dense breasts.

Objective:To understand the activity concentration of 131I aerosol in the air of the iodine treatment workplace and estimate the internal dose of 131I inhaled by medical staff in nuclear medicine. Methods:Using CF-1001BRL portable large capacity air sampler, the 131I aerosol in the iodine treatment workplace of nuclear medicine department of 6 hospitals in Shandong province was collected by iodine box, and the HPGe-γ energy spectrometer was used to measure the samples. The 131I activity concentration in iodine treatment workplace at 6 hospitals was obtained, and the internal dose to medical staff was estimated. Results:The 131I activity concentration in the air in iodine treatment workplaces at 6 hospitals ranged from 3.64 to 2.94×10 3 Bq/m 3. The 131I activity concentration in the controlled area (ward, patient passageway, subpacking room, operation administration room) was significantly higher than that in the supervised area. The highest 131I activity concentration, 2.62×10 2 Bq/m 3, in the supervised area was found in the medical care passageway. The estimated effective dose to nuclear medicine workers was 0.07-5.68 mSv, not exceeding the national limit. Conclusions:The phenomenon of 131I aerosol contamination still exists in the iodine treatment workplaces of nuclear medicine departments in hospitals, so it is necessary to carry out internal radiation monitoring for nuclear medicine departments all around the country, and explore more reasonable protection standards and methods.

Objective To explore the influence of early high-energy-density milk powder feeding on recovery and nutritional status in infants with congenital heart disease after surgery.Methods A total of 82 infants with congenital heart disease were selected and randomly divided into control group(early routine formula feeding)and study group(early high-energy-density formula feeding),with 41 cases in each group.The recovery outcomes,nutritional status,feeding conditions,and cardiac func-tion were compared between the two groups.Results Mechanical ventilation time,treatment duration in Cardiac Intensive Care Unit(CICU),and hospital stay in the study group were significantly shorter than those in the control group(P＜0.05).Compared with preoperative levels,the levels of prealbu-min(PA),retinol-binding protein(RBP),transferrin(TRF)and albumin(ALB)increased signifi-cantly in both groups at 3 months after operation(P＜0.05);at 3 months after operation,the levels of PA,RBP,TRF and ALB in the study group were significantly higher than those in the control group(P＜0.05).Compared with preoperative measurements,both groups showed significant improvements in height and body weight at 3 months after operation(P＜0.05);the height and body weight of the study group were significantly higher than those of the control group at 3 months after operation(P＜0.05).Compared with the control group,the study group had a significant lower rate of inadequate feeding and a significant higher rate of appropriate feeding(P＜0.05).Cardiac function of the study group was significantly better than that of the control group(P＜0.05).Conclusion Early high-en-ergy-density milk powder feeding after congenital heart disease surgery in infants can promote faster recovery,improve nutritional status,and result in more appropriate feeding,with a lower incidence of heart failure.