Objective To study the renal pathology and expression of vascular endothelial growth factor (VEGF) in rats with hypothyroidism caused by iodine deficiency.Methods The rats were randomly grouped into test group and normal group,and the test group was further randomly divided into model group and L-T4 group.Observation were made when the hypothyroid model was formed after maneuvering for 21 days and the medication had been taken for 56 days.The observed data included the morphogy of the kidney,serum TT3,TT4,TSH,blood urea nitrogen (BUN),serum creatinine (SCr),and VEGF expression.Results (1) After medication for 56 days,compared to normal group,BUN and SCr levels in L-T4 and model groups showed apparently increasing [BUN (5.55 ± 0.50,5.80 ± 0.66 vs 5.00 ± 0.24) mmol/L,P＜0.05 ; SCr (26.83 ± 0.75,27.0 ± 3.41 vs 22.5 ± 2.07) μmol/L,P＜0.05],and L-T4 group showed slightly lowered BUN and SCr levels compared with model group (P＞0.05).(2) By 21 days,in the model group there was moderate mesangial hyperplasia involving focal glomeruli.By 56th day of medication,entire glomeruli showed moderate mesangial hyperplasia with lessened number of dilated capillaries in the model group.However,in the L-T4 treated group,mesangial hyperplasia was much less and capillaries though dilated appeared wholesome,as compared with the model group.(3) By 21th day,the IODA value of VEGF in the model group was higher than that in the normal control (44.64 ± 14.99 vs 29.05 ± 9.14,P＜0.01).After medication for 56 days,there was no significant difference in the IODA value of three groups.Conclusions The characteristics of renal pathology in rats with hypothyroidism caused by iodine deficiency consist of mesangial hyperplasia and loss of glomerular capillaries.The expression of VEGF is raised early in the course of renal damage,but lowered thereafter.L-T4 can not reverse the sustained kidney damage and impaired renal function.

Objective To evaluate the relationship between the Coronary Artery Score (CAS) and pulse pressure index (PPI). Methods Coronary angiography was performed in 711 patients, of whom 470 were male and 241 female, and whose age ranged from 30-82 (61.28?8.44) years. Brachial systolic blood pressure (SBP), diastolic blood pressure (DBP), pulse pressure (pp) and PPI were measured. The severity of coronary artery lesions was analyzed by quantitive computer analysis system (QCA) to acquire CAS. Results Age, sex, BMI, SBP, PP, PPI, Ao, the history of essential hypertention, the history of type 2 diabetes, HDL-C were significantly related to CAS. In a Logistic multivariate analysis, PPI and Ao were significantly related to CAS (?=0.041～0.149, P=0.000). Conclusion PPI and Ao are closely related to CAS. They are predictors for patients with coronary artery disease and the results can be used in clinical indication.

The rules of acupoints combination of ancient acupuncture for Xiaoke are mainly explored. By retrieval on ancient literature, the database of acupuncture and moxibustion for Xiaoke is established; based on the database, association analysis between acupoints and symptoms is performed. According to the association analysis in 5 databases of Xiaoke database, Xiaoke database of kidney deficiency, Xiaoke-database of dry mnouth and thirst, Xiaoke database of difficult urination, Xiaoke database of drinking addiction, the results are mainly characterized with symptom differentiation combination, distal-local combination, local combination and front-back combination, which can nourish yin and clear heat. It is believed that establishment of TCM ancient literature database and exploration of data mining technology is a potential research orientation.
Acupuncture Points ; Acupuncture Therapy ; history ; Data Mining ; Databases, Factual ; history ; History, Ancient ; Humans ; Medicine in Literature ; Moxibustion ; history

Objective To observe the clinical efficacy of grain-sized moxibustion in treating cancer-related fatigue and improving the quality of life of patients with malignant tumor. Method Forty-six patients with malignant tumor were randomized into a grain-sized moxibustion group and a control group. The grain-sized moxibustion group received grain-sized moxibustion plus regular treatment, while the control group received the regular treatment only. The cancer-related fatigue intensity [Karnofsky Performance Status (KPS), European Organization for Research on Treatment of Cancer Core Quality-of-life Questionnaire (EORTC QLQ-C30), Piper Fatigue Scale (PFS)], white blood cell count, hemoglobin level, lymphocyte count, and neutrophil count were compared before and after treatment between the two groups. The correlation between cancer-related fatigue and the observed indexes was also analyzed. Result The grain-sized moxibustion group was better than the control group in mitigating the cancer-related fatigue, up-regulating hemoglobin level, and improving the symptoms (P<0.05). Conclusion Grain-sized moxibustion can effectively mitigate cancer-related fatigue via improving anemia condition and the symptoms of malignant tumor patients, and it can also enhance the quality of life of the patients.

OBJECTIVE:To compare the efficacy and safety of Leonurus artemisia cream and Dan’e fukang cream in the treat-ment of primary dysmenorrhea. METHODS:80 patients with primary dysmenorrheal were randomly divided into L. artemisia cream group (40 cases) and Dan’e fukang cream group (40 cases). L. artemisia cream group received L. artemisia cream 10 g, orally,twice a day. Dan’e fukang cream group received Dan’e fukang cream 10 g,orally,twice a day. The treatment was stated before 10 days of menstruation. 10 days was regarded as 1 treatment course,and it lasted for 6 courses. Cold drink and food were forbid during medication,washing face and feet with warm water,soaking feet with hot water if they can,paying attention to keep the lower abdomen and legs warm. If patients felt unbearable pain in menstruation,0.3 g Ibuprofen sustained release capsule was given,orally twice a day. Clinical efficacy,symptom score(dysmenorrhea degree,abdominal pain,lumbosacral pain)before and af-ter treatment,dysmenorrhea duration,frequency of taking painkillers and the incidence of adverse reactions in 2 groups were ob-served. RESULTS:There were no significant differences in the total effective rate and the incidence of adverse reactions in 2 groups (P>0.05). Before treatment,there were no significant differences in dysmenorrhea degree,abdominal pain,lumbosacral pain score,dysmenorrheal duration and frequency of taking painkillers in 2 groups (P>0.05). After treatment,the dysmenorrhea degree,abdominal pain,lumbosacral pain score in 2 groups were significantly lower than before,and Dan’e fukang cream group was lower than L. artemisia cream group with statistical sighificance(P<0.05),and dysmenorrheal duration and frequency of taking painkillers were significantly lower than before,with statistical significance(P<0.05),while there was no significant difference in 2 groups(P>0.05). CONCLUSIONS:Both L. artemisia cream and Dan’e fukang cream have good efficacy and safety in the treat-ment of primary dysmenorrhea,while Dan’e fukang cream is superior to L. artemisia cream in terms of improving clinical symp-toms.

Methods: First, this paper establishes a dataset of abnormal morphology for Chinese medicine diagnosis, with images from public resources and labeled with category labels by several Chinese medicine experts, including three categories: normal, shoulder abnormality, and leg abnormality. Second, the key points of human body are extracted by Light-Atten-Pose algorithm. Light-Atten-Pose algorithm uses lightweight EfficientNet network and polarized self-attention (PSA) mechanism on the basis of AlphaPose, which reduces the computation amount by using EfficientNet network, and the data is finely processed by using PSA mechanism in spatial and channel dimensions. Finally, according to the theory of TCM inspection, the abnormal morphology standard based on the joint angle difference is defined, and the classification of abnormal morphology of Chinese medical diagnosis is realized by calculating the angle between key points. Accuracy, frames per second (FPS), model size, parameter set (Params), and giga floating-point operations per second (GFLOPs) are chosen as the evaluation indexes for lightweighting. Results: Validation of the Light-Atten-Pose algorithm on the dataset showed a classification accuracy of 96.23%, which is close to the original AlphaPose model. However, the FPS of the improved model reaches 41.6 fps from 16.5 fps, the model size is reduced from 155.11 MB to 33.67 MB, the Params decreases from 40.5 M to 8.6 M, and the GFLOPs reduces from 11.93 to 2.10. Conclusion The Light-Atten-Pose algorithm achieves lightweight while maintaining high robustness, resulting in lower complexity and resource consumption and higher classification accuracy, and the experiments prove that the Light-Atten-Pose algorithm has a better overall performance and has practical application in the pose estimation task.

Objective To observe the effect of aerobics exercise on myocardial fibrosis after acute myocardial infarction (AMI) in rat.Methods Twenty-four AMI Sprague Dawley (SD) rats were randomly assigned to sham-operated group (Sham),AMI group and aerobics exercise treatment group (ET).Except Sham,other groups of rats were underwent anterior wall myocardial infarction.After ten weeks,the myocardial mRNA level of connective tissue growth factor (CTGF),collagen Ⅰ (COL1 a1),collagen Ⅲ (COL3a1) detected by real time-quantitative polymerase chain reaction (PCR) analysis and myocardial collagen volume fraction (CVF) was determined on Masson stained sections.Results Campared to Sham-operated group,AMI group the level of CTGF,COL1a1,COL3a1,and CVF were increased (P ＜0.05,P ＜ 0.01).Campared to AMI and ET groups,the levels of CTGF,COL1a1,COL3a1,and CVF were decreased (P ＜ 0.05,P ＜ 0.01).Conclusions Aerobics exercise can reduced the myocardial fibrosis after AMI in rat.

Conditional forebrain-specific presenilin-1 and presenilin-2 double knockout mice (dKO mice) exhibit several neurodegenerative phenotypes of Alzheimer's disease (AD) pathology, such as tau hyperphosphorylation, neuron loss, forebrain cortical shrinkage and memory impairment. By using capillary electrophoresis assay, monoamine neurotransmitters in forebrain cortex, hippocampus and other forebrain region of dKO mice aged at 6, 9 and 12 months were measured to illustrate the relationship among presenilins function deficiency, neurodegenerative phenotypes and monoamine neurotransmitters. Data showed that levels of monoamine neurotransmitters in forebrain cortex of dKO mice were significantly decreased at 6 months when compared to controls, while as mice getting older, levels of monoamine neurotransmitters increased to that of controls, or even higher. In hippocampus, 5-hydroxytryptamin and epinephrine in dKO mice had a significant increase at 6 months, followed with a significant increase of each monoamine neurotransmitter at 12 months age. In other forebrain region, 5-hydroxytryptamin and dopamine had a similar level between control and dKO mice at 6 and 9 months but a significant decrease at 12 months; however, level of norepinephrine and epinephrine were significantly decreased at 6 and 12 months except epinephrine of 6 months. These results demonstrated that knockout of presenilins genes could lead to the variation of monoamine neurotransmitters, and the variation profiles were different among forebrain cortex, hippocampus and other forebrain region. However, whether presenilins deficiency caused the variation of monoamine neurotransmitter directly or not, and how about the effects of variation of monoamine neurotransmitters on AD-like pathology need to be further analyzed.

Objective To analyse the graft impingement after anterior cruciate ligament (ACL) reconstruction based on three-dimensional graft and tunnel images of dual-source computer tomography (DSCT).Methods From November 2012 to November 2014,a consecutive 134 cases of ACL reconstruction with various techniques had their knees scanned by DSCT.Graft and femoral as well as tibial tunnel images were three-dimensionally reconstructed and carefully inspected if there was any impinging on the graft at knee extension,meanwhile the femoral and tibial tunnel location were measured.Of these 134 cases,a serial of 118 patients had been single-bundle reconstructed with same type of graft material.There were 83 males and 35 females aged from 15 to 64 years old (mean,32 years old).Special attention had been paid to examine if there was any graft impinging,and their origin and finally impingement types were identified and classified according to the position and origin of impingement.Bernard and Lorenz method were used to measure the relative locations of femoral and tibial tunnel respectively.Difference was statistically compared between impingement-free and impingement groups.Results Patients were divided into two groups,impingement-free group and impingement group according to graft impinging based on graft visible 3D-CT imaging postoperatively.Of 118 cases,39 (33％) were impingement-free and 79 (67％) existed various types of impingement.Impingement group was further divided into two subgroups according to different impingement location,outlet impingement of intercondylar notch group (77 cases,account for 65％ of total and 97％ of impingement group,respectively) and mid-way impingement of intercondylar notch group (2 cases,account for 2％ of total and 3％ of impingement group,respectively).The outlet impingement group was more further classified into three subtypes,bird beak impingement,10 cases (13％,10/77),tibial plateau impingement,46 cases (60％,46/77) and clamp impingement,21 cases (27％,21/77).Statistic analysis with NAONA revealed that no significant difference between impingement group and impingement-free group regardless of femoral or tibial tunnel placement.Conclusion Three new types of impingements are found based on visible 3D-CT graft imaging after ACL reconstruction which are mid-way impingement of intercondylar notch,tibial plateau impingement and clamp impingement.

Background and purpose: Uridine diphosphoglucu-ronosyl transferase 1A1 (UGT1A1) is an important enzyme for metabolism of irinotecan. The activity of UGT1A1 enzyme was significantly affected by the gene polymorphism. This study aimed to investigate the correlation of UGT1A1*28 and *6 gene polymorphisms with irinotecan-based chemotherapy in colorectal cancer(CRC). Methods: Analysis of UGT1A1*28 and *6 gene polymorphisms was performed in 160 gastrointestinal cancer patients admitted to Zhongshan Hospital Fudan University from Apr. 2013 to Dec. 2013 by amplifying the gene fragments using PCR, STR and Sanger sequencing. Eighty-two cases with CRC treated with irinotecan were chosen to observe the adverse events during chemotherapy. The incidence of different genotypes was compared. Results:The distribution of the genotypes in 160 gastrointestinal cancer patients was as followed:UGT1A1*28 wild-type genotype TA6/6 (124, 77.5%), heterozygous genotype TA6/7 (33, 20.5%), and homozygous genotype TA7/7 (3, 2.0%);UGT1A1*6 wild-type genotype GG (105, 65.6%), heterozygous genotype GA (48, 30.0%), and homozygous genotype AA (7, 4.4%). In the 82 CRC cases, the incidences of grade 3 and 4 neutropenia in the patients carrying UGT1A1*28 (TA6/7+TA7/7 ) were higher than those in the WT genotype (TA6/6) (58.3%vs 0.0, P<0.001), and increased the total incidence of adverse events (76.0%vs 45.6%, P<0.001). There was no signiifcant relevance between UGT1A1*6 genotype, age, gender chemotherapy and adverse events. Conclusion:In the CRC cases with irinotecan-based chemotherapy, the UGT1A1*28 (TA6/7+TA7/7) genotype signiifcantly increased the risk of grade 3 and 4 neutropenia. Detecting UGT1A1 gene polymorphisms may guide individualized treatment and predict adverse events.