Pingyangmycin was produced by Pingyangnesin sp No. 74 which was isolated from mud at Pingyang, Zhejiang China. The structure and properties of Pingyangmycin are the same as those of bleomycin A-g. Pingyangtnycin-induced cellular DNA breakage was observed in this experiment. DNA breakage in vitro was found by agarose gel electrophoresis and UV fluoresene photography after ethidium bromide staining 0. 5ug cellular DNA incubated with a series of drug concentrations for 2h and with a series of drug exposure times at a dose of 200 ug/ml. The mininal concentration and exposure time of DNA breakage is 5 ug/ml and 10 min. DNA molecule was observed under electron microscope with basic protein mounting technique. The natural spiral DNA long strand (X =5.95 um+1.95)was cut down into short DNA fragments ("X =0.75 n.m+0.24)by 10 ug/ml drug for 2h_ The length of untreated DNA is significantly longer than that of the drug treated one(P

Objective To explore the effect of contract learning on rehabilitation training to patients with stroke . Methods According to the random number table, 120 stroke patients were randomly divided in equal number into control group and observation group. The patients in the control group were treated with conventional nursing during hospitalization and health guidance during follow-up. The patients in the observation group were given rehabilitation training according to contracted learning during the period of hospitalization and follow-up. The time was 6 months. The quality of life was compared between the two groups of patients before and after the intervention, 1 months after discharge, 6 months after discharge. Results After six months of intervention, the 7 dimensions of quality of life, including daily life ability, hand function, mobility, strength, emotion, communication and participation in the intervention group were significantly better than those of the control group (P<0.001). The 6 dimensions of quality of life, including daily life ability, hand function, mobility, strength, emotion, and communication of the control group were significantly better than before the intervention (P<0.001). Conclusion For stroke patients, the rehabilitation training under the learning contract can improve the effect of rehabilitation training so as to improve the quality of life and the prognosis.