The ultrasound devices have been widely applied in hospitals.The audio & video teaching system for ultrasonic diagnosis based on network was built for training ultrasonic diagnosis skill.The system should lay emphasis on the design of consulting room,network,server,grouping study and meeting teaching systems.The system construction and application are introduced in combination with actual experiences in hospital.

According to information interchange situation in our hospital,this article analyzes and discusses the PHS,GSM,CDMA and DECT technologies,selects the PHS technology to construct hospital mobile communication system for the better construction of hospital information interchange environment.

Objective:To analyze the value of cerebrospinal fluid (CSF) lactic acid level and lactic acid clearance rate in arterial blood in predicting the prognoses of patients with aneurysmal subarachnoid hemorrhage (aSAH).Methods:A total of 70 patients with aSAH admitted to our hospital from August 2018 to October 2019 were selected. The clinical data and biochemical indexes of cerebrospinal fluid were collected, and the study subjects were re-examined and followed up 3 months after operation. The patients were divided into good prognosis group and poor prognosis group according to modified Rankin scale (mRS) scores. Univariate analysis was used to compare the clinical data and biochemical examination indicators of patients from the two groups. Multivariate Logistic regression analysis was used to screen the independent factors affecting the prognoses of the patients.Results:There were 51 patients in the good prognosis group and 19 patients in the poor prognosis group. The patients in the good prognosis group had significantly younger age, significantly higher Glasgow coma scale (GCS) scores, and significantly better Hunt-Hess grading and modified Fisher classification distribution than those in the poor prognosis group ( P<0.05). The contents of CSF lactic acid at 0-24 h and 48-72 h after surgery in the good prognosis group were significantly lower than those in the poor prognosis group ( P<0.05); the percentages of patients with CSF lactic acid≤3.28 mol/L and patients with CSF lactic acid clearance≥10% were significantly higher than those of poor prognosis group ( P<0.05). Multivariate Logistic regression analysis showed that age ( OR=1.089, 95%CI: 1.000-1.186, P=0.049), GCS scores ( OR=5.610, 95%CI: 1.136-27.696, P=0.034), Hunt-Hess grading ( OR=3.921, 95%CI: 1.084-14.175, P=0.037), and CSF lactic acid clearance rate ( OR=9.821, 95%CI: 1.278-75.483, P=0.028) were independent factors affecting the prognoses of aSAH patients. Conclusion:CSF lactic acid clearance rate can predict the postoperative outcome of aSAH patients; patients with CSF lactic acid clearance rate≥10% have a good prognosis.

Objective To explore the microRNA-16 (miR-16) and nuclear-transcription factor-κB1 (NF-κB1) expressions in human brain gliomas and their correlations with cell invasion and growth of malignant gliomas SHG44,U87 and U373.Methods (1) Twenty-nine cases of human glioma tissue samples and 6 normal brain tissues,collected in our hospital from January 2000 to January 2011,were chosen in our study; quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the expressions ofmiR-16 and NF-κB1 in these tissues.(2) In vitro cultured U87,U373 and SHG44 cells were divided into blank-control group,nonsense sequence transfected group and miR-16 mimics transfected group; 48 h after the transfection,qRT-PCR was used to detect the expressions of miR-16 and NF-κB1; tmnswell assay was used to observe the cell invasion capability; 72 h after the transfection,Western blotting was employed to detect the protein expressions of NF-κB1,matrix metallopeptidase 9 (MMP-9) and MMP-2.(3) Luciferase reporter assay was used to detect the target regulating role of miR-16 in NF-κB1 gene.(4) U87 cells were used as negative control group,and U87 cells carried stably expressed miR-16 gene were implanted into intracranial and subcutaneous nude mice (U87-miR-16 group); immunofluorescence was used to detect the MMP-9 expression,and immunohistochemical staining was used to detect the protein expressions of Ki-67,NF-κ B1 and MMP-9; subcutaneous tumor volume was measured and the growth curve was drawn.Results (1) The qPCR results showed that the expression of miR-16 in human brain glioma tissues was significantly lower than that in normal brain tissues; and gradually decreased miR-16 expressions were noted in gliomas of graded Ⅰ,Ⅱ,Ⅲ and Ⅳ (P＜0.05); NF-κB1 expression in human brain glioma tissues was significantly higher than that in normal brain tissues; and gradually increased NF-κB1 expressions were noted in gliomas of graded Ⅰ,Ⅱ,Ⅲ and Ⅳ (P＜0.05).(2) As compared with those in the blank-control group and nonsense sequence transfected group,miR-16 mimics transfected group had significantly increased miR-16 expression,decreased NF-κB1 mRNA expression,decreased invasiveness,and decreased protein expressions of NF-κB1 and MMP-9 (P＜0.05).(3) Luciferase reporter assay showed that the fluorescence normalized ratio in the pMIR-NF-κB1 group was signfcaintly higher than that in the pMIR-NF-κB1+pre-miR-16 group (P＜0.05).(4) As compared with the negative control group,the U87-miR-16 group on the 24-42 d of implantation had significantly smaller volume of tumors (P＜0.05),and lower MMP9 expression,and NF-κB1,MMP-9 and Ki-67 expressions (the proliferation index of Ki-67:13.91％ and 32.98％).Conclusion MiR-16 inhibits glioma cell invasion and growth through down-mgulating NF-κB1 and MMP-9 expressions.

Objective:To explore the influences of times of venous thromboembolism (VTE) drug prophylaxis in formation of deep vein thrombosis (DVT) in patients with severe craniocerebral injury after surgical treatment.Methods:Ninety patients with severe craniocerebral injury, admitted to our hospital from February 2021 to December 2021, were chosen in our study; they were divided into early group ( n=47, less than 48 h from the time of admission) and late group ( n=43, more than 48 h from the time of admission) according to the times of initiation for VTE drug prophylaxis (low molecular weight heparin calcium injection [LMWH] 100 IU/Kg was injected subcutaneously once a d). One week after injection, the DVT formation in the lower limbs, intracranial rebleeding, gastrointestinal bleeding and death were compared in the two groups. Results:The times of initiation for drug prophylaxis in the early group and late group were (28.91±4.50) h and (71.56±8.89) h. The DVT formation in the early group was significantly lower than that in the late group (12.8% vs. 34.9%, P<0.05). There was no difference in the incidence of intracranial rebleeding, mortality or gastrointestinal bleeding between the two groups ( P>0.05). Conclusion:Early initiation of VTE drug prophylaxis can significantly reduce the incidence of DVT in patients with severe craniocerebral injury after surgical treatment, enjoying high safety.