Objective:To optimize the extraction process of total flavonoids from Chloranthus japonicus sieb by Box-Behnken re-sponse surface methodology. Methods:The independent variables were the ethanol concentration ( X1 ) , liquid-solid ratio ( X2 ) and extraction time ( X3 ) , the dependent variable was the extraction amount of total flavonoids ( Y) , and the extraction process was opti-mized by Box-Behnken response surface methodology. Results: The results showed that the optimal extraction conditions for total fla-vonoids from Chloranthus japonicus sieb were as follows: the ethanol concentration was 54. 8%, the liquid-solid ratio was 13. 6, and the extraction time was 2. 0 h. The verified extraction process parameters of three batches showed that the relative error between the ex-perimental values and those predicted from the regression model was -2. 34%. Conclusion:The optimal extraction process parameters are simple and more convenient with higher precision for the extraction of total flavonoids from Chloranthus japonicus sieb.

OBJECTIVE： To establish the quality standard of Tricyrtis maculata. METHODS： The character and microstructure of T. maculata were identified according to the method stated in 2015 edition of Chinese Pharmacopoeia. The contents of moisture， ash and extract were determined. TLC was used for qualitative identification of quercetin and nicotifiorin in samples. The contents of puerarin， p-hydroxybenzoic acid， nicotiflorin， ferulic acid， quercetin and kaempferol were determined by HPLC. RESULTS： The characters and microscopic identification had specificity. TLC spots of quercetin and nicotifiorin were clear and well-separated without interference from negative control. The water content， total ash content， acid insoluble ash content， alcohol soluble extract and water soluble extract for 10 batches of samples were 0.61％-1.89％，6.28％-8.88％，0.76％-1.79％，1.31％-2.00％ and 9.39％-14.27％， respectively. The linear range of puerarin， p-hydroxybenzoic acid， nicotiflorin， ferulic acid， quercetin and kaeniphenol were 0.031 92-0.111 7 μg （r＝0.999 6）， 0.085 3-0.298 5 μg （r＝0.999 5）， 0.010 76-0.037 66 μg （r＝0.999 8）， 0.070 08- 0.245 3 μg （r＝0.999 8），0.058 56-0.205 0 μg （r＝0.999 4），0.009 860-0.033 88 μg （r＝0.999 4）， respectively. The quantitation limits were 1.06， 0.47， 0.75， 1.40， 1.20， 0.74 ng， and the detection limits were 0.36， 0.12， 0.30， 0.53， 0.60， 0.31 ng， respectively. RSDs of precision， stability and repeatability tests were all less than 2％. The average recoveries were 101.54％， 102.10％， 101.46％， 103.35％， 99.36％ and 96.85％， respectively； RSDs were 1.76％， 1.68％， 1.56％， 1.26％， 0.91％ and 1.96％， respectively （n＝6）； the results of the content were 0.017-0.047， 0.042-0.140， 0.003 8-0.015 0， 0.049-0.180， 0.024- 0.091， 0.003 9-0.011 0 mg/g. CONCLUSIONS： The established quality standard can be used for the quality control of T. maculata.