1.Several ways of actively promoting harmonious health
Chinese Journal of Hospital Administration 1996;0(10):-
That at present medical and health services are both inaccessible and expensive is an issue that health departments in charge are predominantly concerned with and must provide a solution to. For this reason, the Health Bureau of Nanjing has devoted major efforts to the following aspects: rational allocation of health resources; vigorous development of urban community health services; earnest improvement of rural health work; active promotion of medical benefits to the masses of people; full adoption of drug purchases via bidding; energetic support for nongovernmental medical institutions; vigorous crackdown on corruptive trade practices; active promotion of highly civilized health industries and speedy improvement of the medical service environment.
2.Study Status of Quality Control and Analysis Methods for Vitamin C Injections
Chunyan SUN ; Tianming DING ; Ninglin CHEN
China Pharmacist 2014;(5):868-870
Vitamin C injections are a national essential drug with wide clinical applications and instable property. In order to pro-vide the reference for further analysis on the quality status of vitamin C injections and improve the quality,the research on the stability, quality control and analysis methods was summarized in the study.
3.Experimental Study on Treatment of Acute Radiation Sickness in Dogs
Yongtang YAN ; Tianming CHEN ; Qingjia HE
Journal of Third Military Medical University 1984;0(01):-
Seventy dogs were irradiated -with 300. 325, or 350 rad of gamma rays from a 60Co sourse. Half of them were used as controls and the other 35 animals were divided randomly into four groups and were experimentally treated with different regimes.In the first group there were 9 treated, animals and an equal number of control animals 5 all the animals were irradiated with 300 rad. The treatment consisted of a blood transfusion on the 16th day after radiation exposure as well as several antibiotics and other medications. 8 out of the treated animals and one of the controls survived the gamma irradiation.In the second group, ten animals were exposed to 350 rad and 8 to 325 radand they were divided equally into treated and control groups. In spite of early use of blood transfusion and antibiotics, all the treated animals as well as those of the control group died of severe mycotic infections.In the third group, 325 rad was given to the dogs. All 7 dogs of the controls died and two out of the 7 treated dogs survived. The treatment was the same as that of the first group but no blood transfusion was used.In the fourth grou'p?ten animals were irradiated with 350 rad and another ten with 325 rad. They were divided equally into control and treated groups. All the ten controls died. The treated group was given the same treatment as the first group. In addition, tetracycline was given in case there was fever in 'the critical phase of the sickness, and the dosage of tetracycline was doubled whenever fever persisted or recurred. All the treated animals survived.Early blood transfusion was found to be better in its therapeutic effect than late transfusion.
4.NEW DISCOVERY: REPORT OF A HUMAN SCHISTOSOME
Guoyu XU ; Tianming CHEN ; Jiequa LI
Chinese Journal of Schistosomiasis Control 1989;0(02):-
Objective To delineate the m orp hology and ecology of a new human schistosome species. Methods (1)Adult schistosomes were obtained from infected mice and rabbi ts with cercariae shedding from snails which were infected with miracidia devel oping from the eggs in feces of acute schistosomiasis patients. (2)T he rabbits were dissected during 60-100 d infection period of cecariae, and thei r bladders were taken out for detecting eggs in the urine. (3)Schistosome eggs i n the feces of patients were examined with microscopy. Results (1)There were two types of male worms: one was with an average o f size 8.76 mm long and 0 43 mm wide while the other with an average size of 2 41 mm long and 0 091 mm wide and with undeveloped gynecophoral canal which ap peared in front of ventral sucker (2)There were two types of female worms: one was with an average of size 10 73 mm long and 0 74 mm wide while the other wi th an average of size 3 43 mm long and 0 12 mm wide and was fine, semitranspar ent and tape like with undeveloped ovary and vitellaria. Less than 10 eggs or none were found in t he uterus. (3)There were a lot of degenerated and black eggs in the feces of 2 p atients. (4) Schistosome eggs were detected in the urine and miracidia were hatc hed out from 8 out of 16 infected rabbits.(5) Others: 8 worms were detected in 1 mouse out of 12 dissected mice for evaluation of infectious water with sentry m ice in spite of that no Oncomelania snails were found in the area. Cercariae stayed on water surface in lying position like a c omma. The eggs returned forwardly a fter growing behind the ovary in female worm. ConclusionAccording to the primary investigation in morphology an d ecology, the new schistosome obtained is distinct from the 6 well defined ty p es of human schistosome. It is considered that a new schistosome species should be foun d and temporarily nominated as Schistosoma nanjingi( S.n.)
5.The pathological change in transitional mucosa adjacent to the rectal cancer and its clinical significance
Pan CHI ; Tianming HU ; Daliang CHEN
Chinese Journal of General Surgery 1993;0(02):-
Objective To investigate the biopathologic characteristics of transitional mucosa (TM) adjacent to rectal cancer and its relationship to the anastomotic recurrence after curative surgery of rectal cancer. Method The expression of p53,p21 ras and PCNA proteins was determined immunohistochemically in primary rectal adenocarcinomas (n=67), the proximal margins (n=67), the distal margins (n=67) and rectal mucosa 1~3?cm distant to the anastomosis in patients one year after radical resection (n=33), and two years after (n=31).Results The expression of TM, p21 ras and p53 proteins,and PCNA index in primary rectal adenocarcinomas was higher than that in two resection margins (P0.05). Positive TM was often found in the area of chronic inflammation. conclusion TM adjacent to the rectal cancer is a nonspecific, not cancer precursor event.
6.Advantages of cemented dynamic hip screw for osteoporotic intertrochanteric fracture
Yuanshi SHE ; Guangxiang CHEN ; Guangdong CHEN ; Yijin WANG ; Tianming ZOU
Chinese Journal of Tissue Engineering Research 2014;(26):4158-4162
BACKGROUND:Dynamic hip screws have been considered as a classic method for intertrochanteric fracture. However, migration of the hip screw resulting in cut-out of the femoral head remains the most common mechanical failure, which requires secondary surgery. OBJECTIVE:On the basis of the traditional dynamic hip screw, we modified the lag screw, designed new internal fixation system as cemented dynamic hip screws to explore the feasibility and the biomechanical properties for the treatment of osteoporotic intertrochanteric fracture. METHODS:A total of 24 hip samples were harvested from fresh cadavers with osteoporosis. Bone density was measured. According to the standards, the specimens were made into intertrochanteric fracture models of AO31-A1 type. Of each specimen, one side implanted with cemented dynamic hip screws served as experimental group, and another side implanted with traditional dynamic hip screw served as control group. Axial compression, twisting and fatigue load biomechanics were tested. Their biomechanical functions were compared. RESULTS AND CONCLUSION:Stress intensity, axial stiffness and horizontal shear stiffness were higher in the experimental group than in the control group (P<0.05). At torsion angle of 3°, torsional moment was higher in the experimental group than in the control group (P<0.05). Fatigue load was higher in the experimental group than in the control group (P<0.05). Results suggested that cemented dynamic hip screw internal fixation system in the treatment of senile osteoporotic intertrochanteric fracture can effectively enhance the hip screw holding force and prevent femoral cutting. Biomechanical property, stiffness, endurance resistance and stability of cemented dynamic hip screw are better than traditional dynamic hip screw.
7.Impact of supine recumbency duration on the complications after lumbar puncture in children
Weiling YAN ; Zhenzhen DOU ; Tianming CHEN ; Huili HU ; Gang LIU
Chinese Journal of Applied Clinical Pediatrics 2014;29(12):914-918
Objective To evaluate the post-puncture complications in children,and to evaluate the difference in the occurrence rate of headache and backache between patients who had 1 hour of supine recumbency and those who had 4 hours of supine recumbency,to provide evidence for the standardized lumbar puncture (LP) procedure of children.Methods Inpatients who were older than 3 and had diagnostic LP in the course of their treatment between Nov.2012 and Apr.2013 were enrolled.The LP was performed by the same investigator under the standardized institutional guideline for LP and the information of number of LP attempts,duration of LP and cerebrospinal fluid (CSF) drainage volume were recorded.After the procedure,all the children were randomly assigned into 2 groups:the test group had supine recumbency for 1 hour after LP,and the control group had it for 4 hours.For the first 5 days following LP,reports of headache and backache were recorded by another physician who didn't participate in this study.The difference in the occurrence rate of headache and backache between test group and control group was assessed by the chi-square test or Fisher's probabilities in 2 × 2 table.And a Logistic regression analysis was performed to assess the risk factors for the occurrence of the headache and backache.Results The overall frequency of headache was 4.6% (4/87 cases).The frequency of headache was not significantly different between the test group (2.4%) and control group (6.7%) (P =0.617).And the overall frequency of backache was 19.5 % (17/87 cases),and it was not significantly different between the 2 groups (test group:21.4%,control group:17.8 %,P =0.668).In a Logistic regression analysis,age (P =0.011,OR:6.884,95% CI:1.398-33.906) and the previous history of lumbar puncture (P =0.018,0R:0.126,95 % CI:0.026-0.618) were significant risk factors for the occurrence of backache.The risk of backache in children with more than 2 times of LP was decreased.And the risk was higher in the children older than 6 years than those younger than 6 years.Conclusions Headache and backache were the most frequent post-puncture complications in children.There was no difference between short duration (1 hour) of supine recumbency and long duration (4 hours) in preventing the occurrence of headache and backache after LP.
8.Effects of matrine on apoptosis and related protein expression in human medulloblastoma D341 cells
Kaiyu ZHOU ; Tianming MAO ; Xi CHEN ; Yongkang LUO
Chinese Journal of Pathophysiology 2014;33(4):629-634
AIM:To investigate matrine-induced apoptosis of human medulloblastoma D 341 cells and the ex-pression of Bax , Bcl-2, serine/threonine kinase Akt and phosphorylated Akt ( p-Akt) in vitro.METHODS: D341 cells were divided into experimental groups ( added with matrine at different concentrations ) and control group ( under the same conditions without matrine).The proliferation of D341 cells was analyzed by CCK-8 assay.Apoptosis was detected by An-nexin V-FITC/PI double staining and the expression of Bax , Bcl-2, Akt and p-Akt was detected by Western blotting .RE-SULTS:Matrine significantly inhibited the proliferation of D 341 cells and increased the apoptosis in a dose-and time-de-pendent manner .The cell apoptosis was characterized by chromatin condensation with margination of chromatin to the nu -clear membrane , increased when and larger cytoplasmic vacuoles , and formation of apoptotic body after treatment with ma-trine.The expression of Bax increased , while the expression of Bcl-2 and p-Akt decreased when the drug concentration gradually increased .CONCLUSION:Matrine induces the apoptosis of human medulloblastoma D 341 cells in vitro by acti-vation of Bax, down-regulation of Bcl-2 and reduction of p-Akt expression level in the PI3K/Akt signaling pathway.
9.Extraction of fish scale collagen and its thermal stability:research progress
Tianming DU ; Zihao CHEN ; Xiangyu TANG ; Jimin WU
Military Medical Sciences 2015;(1):75-78
In recent years, researchers have become more interested in fish scrap collagen collection .Fish collagen, thanks to its low antigenic and hypoallergenic properties , is superior to that of higher animals .Meanwhile , fish collagen derives from a wealth of sources and has a moderate price .Based on recent fish collagen extraction and modification , this article summarizeds extraction technologies , such as hot-water, chemistry, bio-enzyme, compound extraction and such modification methods as physical and chemical processes to improve the thermal stability .
10.Nitric Oxide Inhibits Delayed Rectifier Potassium Currents in Cultured Hippocampal Neurons via S-Nitrosylation
Chunfei WANG ; Ming CHEN ; Manlung FUNG ; Tianming GAO
Progress in Biochemistry and Biophysics 2006;0(03):-
The modulating action and mechanism of endogenous nitric oxide (NO) on the delayed rectifier potassium currents in cultured hippocampal neurons were examined using whole-cell patch clamp techniques. L-arginine (L-Arg, 2 mmol/L), a substrate of NO synthases, significantly suppressed the delayed rectifier K+ currents in hippocampal neurons, while its isomer D-arginine (D-Arg, 2 mmol/L) exerted no effect. Moreover, pretreatment with NO synthase inhibitor L-NAME (0.5 mmol/L) completely blocked the suppressing effect by L-Arg, indicating that L-Arg exerted its modulation by producing NO but not by itself. No effect was found on the L-Arg-induced inhibition by 10 min pretreatment of 10 |?mol/L ODQ (a specific inhibitor of guanylate cyclase). In contrast, thiol-alkylating agent N-ethylmaleimide (1 mmol/L) completely precluded L-Arg-induced inhibition on the whole K+ currents. The results indicate that endogenous NO modulates the delayed rectifier K+ currents in cultured hippocampal neurons mostly through S-nitrosylation.