Objective To observe the effect of treating Diabetic Peripheral Neuropathy with Acupoint Injection. Methods 65 patient with DPN were randomly recruited into a control group and a treatment group after adjusting stage. The control group was treated with acupuncture, while the treatment group was treated with the Safflower inoculation fluid acupuncture point injects. Nerve function parameter (MDNS、 NCV) and clinical symptoms were observed after 3 therapeutic courses. Results Nerve function parameter (MDNS、 NCV) and clinical symptoms in the treatment group were apparently improved after treatment (P<0.05) . Conclusion Acupuncture point injected with Safflower inoculation fluid may improve clinical symptoms and nerve functions of DPN patients.

Objective: To study the effect of Musashi-2 ( MSI2 ) overexpression on the imbalance of proliferation and apoptosis of human ovarian granulosa cell line (KGN) induced by dihydrotestosterone (DHT) ． Methods: The gene expression profiles of human ovarian granulosa cells ( GCs) in primary culture were statistically analyzed to screen the differentially expressed genes．pcDNA3. 1-MSI2 eukaryotic expression plasmid was constructed and transiently transfected into the KGN cells，and the overexpression effect of MSI2 was detected by Quantitative Real-time PCR (RT-qPCR) and Western blot．After overexpressing MSI2 in DHT induced KGN cells，MTT colorimetry and Edu staining were used to detect the proliferation of cells in each group，and flow cytometry ( FCM) was further used to detect the apoptosis of cells in each group. Results: The mRNA expression level of MSI2 gradually decreased during the primary culture of human ovarian GCs．And pcDNA3. 1-MSI2 was successfully constructed and transfected into KGN cells to improve the mRNA and protein expression levels of MSI2．Then MTT，EdU and FCM results showed that compared with the blank group，DHT induction could significantly reduce the proliferation rate and increase the apoptosis rate of KGN cells (P ＜0. 05) ．However，after MSI2 overexpression，the proliferation rate of KGN cells increased and the apoptosis rate decreased (P ＜0. 05) ，which were close to the blank group. Conclusion Overexpression of MSI2 can effectively alleviate the imbalance of proliferation and apoptosis of KGN cells induced by DHT，indicating that MSI2 plays an important role in GCs growth and follicle development.

Objective : To investigate the clinical effects and pregnancy outcomes of using luteal phase long protocol and GnRH antagonist protocol in patients with polycystic ovary syndrome (PCOS) who have failed their first GnRH antagonist protocol therapy. Methods : The clinical data of 163 PCOS patients who underwent IVF/ICSI⁃ET were retrieved. After the failure of their first GnRH antagonist protocol treatment , they were divided into two groups in the second controlled ovarian hyperstimulation (COH) cycle : Luteal phase long protocol group ( n = 95) and GnRH antagonist protocol group (n = 68) . A retrospective analysis and comparison of basic clinical data , clinical and laboratory indicators , and pregnancy outcomes between two groups were conducted. Results : ① There was no statistically significant difference in basic clinical indicators between two group except LH. ② Compared the first and second cycle treatments of patients in the luteal phase long protocol group , the initiation dose of gonadotropin (Gn) , total number of Gn days , total Gn usage , estradiol (E2 ) on the day of hCG injection , number of retrieved eggs , oocyte maturation rate , 2PN fertilization rate , 2PN cleavage rate , blastocyst formation rate , high⁃quality blastocyst formation rate , and moderate to severe OHSS rate were significantly higher than those in the first GnRH antagonist cycle (P < 0. 05) . The GnRH antagonist protocol group also showed similar improvements. ③ The comparison of the second COH cycle between two groups showed that the total number of Gn days , total Gn usage , and total Gn cost in the luteal phase long protocol group were significantly higher (P < 0. 05) , while the E2 and LH on the day of hCG injection , and the maturation rate of eggs were significantly lower than those in the GnRH antagonist protocol group ( P < 0. 05) . However, there was no statistically significant difference in the number of retrieved eggs , 2PN fertilization , 2PN cleavage , blastocyst formation rate , high⁃quality blastocyst formation rate , and OHSS rate between the two groups ; ④ The comparison of fresh transplantation cycles for the second COH cycle between the two groups showed that the luteal phase long protocol fresh transplantation rate , implantation rate , clinical pregnancy rate , and live birth rate were slightly higher than those of the GnRH antagonist protocol group , but the difference was not statistically significant. Comparing the outcomes of pregnancy following the initial frozen⁃thawed embryo transfer (FET) between two groups , the biochemical pregnancy rate and clinical pregnancy rate of the GnRH antagonist protocol group were higher than those of the luteal phase long protocol group (P < 0. 05) . However, no significant statistical variations were found in implantation rate , live birth rate , neonatal gestational age , and birth weight. Conclusion For PCOS patients who fail the first GnRH antagonist protocol , an appropriate increase in the initiating dose and usage of Gn can achieve satisfactory pregnancy outcomes with both protocols. Compared with change to a luteal phase long protocol , reusing the GnRH antagonist protocol still maintains its long⁃standing advan⁃ tages , such as shorter total Gn days , lower costs , and better patient compliance.