1.The influence of EBV infection on p53 expression in gastric carcinoma patients from Tangshan area
Shuying LI ; Jinlan GAO ; Qingan XIA ; Tianji ZHOU
Cancer Research and Clinic 2001;0(02):-
Objective To investigate the influence of EBV infection on the expression of tumor suppressor gene p53. Methods EBER1 in 217 cases of gastric carcinoma were detected with in situ hybridization. Then, EBV positive and negative cases were selected for analysis of the expression of p53 by immunohistochemistry. Results In 217 cases of gastric carcinoma, there were 23 cases with EBER1 positive. The average area (AA), mean absorbency and integral absorbency of p53 expression were higher in EBV positive gastric carcinoma than those in EBV negative. Conclusions EBV infection of gastric carcinoma is closely related to the expression of p53 in this study.
2.Anti-cytomegalovirus (CMV) effect of antisense oligodeoxynucleotides phosphorothioates (AS S OND)
Tianji ZHOU ; Xueyi ZHANG ; Hao ZHANG ; Lijun WANG ; Zhongyuan RENG ;
Chinese Pharmacological Bulletin 1986;0(04):-
AIM To study anti CMV effect of S ODNs complementary to the initial code region of major immediate early (MIE) mRNA, and to the intron1/exon2 boundary of MIE mRNA precursor. METHODS To evaluate the anti CMV effect by determining viral antigen on infected 2BS cells by in situ ELISA. RESULTS Both of 2 AS S ODNs and their sense sequence control showed anti CMV effect. The medium effective concentration (EC 50 ) were 4 53, 10 2, 26 2 and 30 1 ?mol?L -1 . The secretion of CMV antigens were delayed by 3 d～4 d by 8 0 ?mol?L -1 AS 1. It showed increased effect by administrating earlier postinfection, by supplementing the S ODN at intervals, by conbinating with ganciclovir, and by packaging with liposome. A slight cytotoxicity was observed at the concentration of 64 0 ?mol?L -1 . Northern blot analysis indicated that the MIE mRNA decreased after the treatment of AS 1. It suggests that disintegration of the mRNA in the hybridized duplex by activated RNase H played an important role as the mechanism of specific action, and "time and effect" analysis suggested that interference of viral adsorbtion and penetration may be the important mechanism of nonspecificity. CONCLUSION AS S ODNs targeted to MIE gene are effective anti CMV agents which can be developed as a new type of chemotherapy drugs against CMV infection.
3.Epidemiological characteristics of human plague in different age groups in China from 1950-2012.
Cheng JU ; Zhencai LIU ; Guijun ZHANG ; Cheng XU ; Xiaoheng YAO ; Tianji DUAN ; Lei CHEN ; Xiaolei ZHOU ; Xianbin CONG
Chinese Journal of Epidemiology 2014;35(1):101-103
Adolescent
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Adult
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Age Distribution
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Aged
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Aged, 80 and over
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Child
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Child, Preschool
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China
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epidemiology
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Humans
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Middle Aged
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Plague
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epidemiology
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Yersinia pestis
4.A Case Report of Aicardi-Goutières Syndrome Type 7 Caused by IFIH1 Gene Mutation and a Literature Review
Min ZHAO ; Zhou SHU ; Tongxin HAN ; Yanhua FU ; Tianji GAO ; Huawei MAO
JOURNAL OF RARE DISEASES 2024;3(4):453-460
To explore the clinical and genetic features of Aicardi-Goutières syndrome (AGS) caused by We analyzed the clinical features and genetic mutation results of a boy with AGS type 7 and conducted a retrospective review of the literature of the characteristics and clinical features of In the case of this report, the patient, 13-year-old boy, exhibited gait abnormalities at age 3. As the condition was progressive, the boy has paraplegia of the lower limbs. The first brain MRI showed no lesions.Rehabilitation therapy in the past several years has shown no improvement.Recent brain CT revealed multiple intracranial calcifications. The whole-exome sequencing identified a heterozygous mutation in the AGS7 is a type of I interferonopathy. Growth retardation and nervous system involvement are the most prevalent.The condition usually involve the skin, blood system, digestive system, kidney, heart, and other organs. JAK inhibitors prove effective for this disease.
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