1.Analysis of diagnosis and treatment of 18 patients with primary presacral tumor
Tianfu WANG ; Danian HUANG ; Bin LIU
Chinese Journal of Postgraduates of Medicine 2017;40(5):438-441
Objective To investigate the diagnosis and treatment of primary presacral tumor. Methods The clinical data of 18 patients with primary presacral tumor were retrospectively analyzed. Results Preoperative diagnosis of primary presacral tumor depended on digital rectal examination, endorectal ultrasound, CT, MRI, et al. The surgical approaches of 18 cases included posterior approach (14 cases) and abdominoperineal approach (4 cases). All tumors were completely resected. Postoperative complications were rectal injury (1 case) and wound infection (2 cases), which were cured by symptomatic treatment. Postoperative pathological results showed that 15 cases had benign lesions and 3 cases had malignant lesions. Sixteen patients were followed up from 0.5 to 5.0 years, with recurrence in 2 cases and death in 2 cases. Conclusions Primary presacral tumor should be treated with operation. Sufficient preoperative examination, personalized operative planning, subtle manipulation operative procedures with an experienced multidisciplinary team, are the important points in preventing or reducing recurrence. Endorectal ultrasound plays an important role in the diagnosis and treatment of primary presacral tumor.
3.Bronchoscopy in the application of emergency treatment of infantile acute fibrinous laryngotracheobronchitis.
Tianfu LIU ; Shubei REN ; Xu ZHU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(16):1484-1485
Bronchitis
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therapy
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Bronchoscopy
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Emergency Treatment
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Humans
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Infant
4.Construction of LAT1 eukaryotic expression vector of C57 mouse and its effect on the Neuro-2a cell
Bingyan WEI ; Tianfu LIU ; Linhua FAN ; Maolin LIU
Chinese Journal of Comparative Medicine 2014;(5):25-30,9
Objective To construct the lamino acid transporter 1 eukaryotic expression vector of C 57 mouse and to express the gene inNeuro-2atumor cells,and explore the effect of LAT1on proliferation and apoptosis of Neuro-2a cell. Methods The full-length LAT1 cDNA was synthesized by RT-PCR and cloned into pcDNA3.1vector to construct recombinant plasmid.The constructed pcDNA3.1-LAT1vector was verified by Enzyme digestion and sequencing and then transfected intomurine Neuro-2acellsby liposome.The transfected cells were selected with G418 and stably expressed strain was constructed .The expression of LAT1 was detected by RT-PCR and western blot .Proliferation was analyzed by MTT , cell cycle and apoptosis were detected by flow cytometric analysis .Results The full-length LAT1 cDNA was amplified successfully and pcDNA3.1-LAT1eukaryoticvector was constructed successfully .Enzyme digestion and sequencing confirmed the sequence was correct .Neuro-2acells were transfected and Stably expressed strain was constructed successfully.MTT showed that the group of transfected restructuring plasmid could significantly affect Neuro -2a cell proliferation more than the control groups ( P <0.05 ) .From the flowcytometric analysis , LAT1 could promote cell proliferation and inhibit Neuro-2a cell apoptosis.Conclusion LAT1 can express successfully inNeuro-2acells which were transfected with recombinantpcDNA3.1-LAT1plasmid.LAT1 in Neuro-2a cells can promote cell proliferation and inhibit the cell apoptosis which provides a basis for the study of LAT 1.That lays the foundation for studying biological effects of LAT 1.
5.Comparison of stress distribution in abutment and alveolar ridge between two kinds of distal-extention absence dentures
Wei WANG ; Tianfu ZHANG ; Ying LIU ; Xuxi QIN
Journal of Jilin University(Medicine Edition) 2006;0(03):-
Objective To analyze the stress distribution of abutment and alveolar ridge between two kinds of distal-extention absence dentures,and provide evidence for the application of different dentures in clinic.Methods Attachment denture and cantilever fixed bridge were used to restore the distal-extention absence respectively,the stress distribution of abutment and alveolar ridge was compared by three-dimensioal finit element method.Results The stress peak values of abutmen root,periodontal membrance,alveolar ridge were(1.42E+5)MPa,(1.33E+4)MPa and(3.49E+5)MPa,respectively,in attachment denture;while in cantilever fixed bridge,the stress peak values of them were(1.45E+7)MPa and(2.25E+6) MPa,(1.45E+3)MPa,respectively.And the stress peak values of these two kinds of dentures were all located in the mid-upper 1/3 and the cervical of abutment.Conclusion Compared with cantilever fixed bridge,the attachment denture could decrease the stress of abutment and be helpful to its protection,the hight of residual alveolar ridge should be taken into account when the two kinds of dentures are used for distal-extention absence.
6.Effect of abnormal glucose metabolism on the expression of macrophage migration inhibitory factor and JNK in the rat liver
Linhua FAN ; Jianxin LIU ; Dan LI ; Bingyan WEI ; Maolin LIU ; Chunfang WANG ; Tianfu LIU
Acta Laboratorium Animalis Scientia Sinica 2015;(2):165-170
Objective To investigate the expression changes of macrophage migration inhibitory factor( MIF) and C-Jun N-terminal kinase ( JNK) in the liver of rat with abnormal glucose metabolism and explore the pathological mecha-nism of abnormal glucose metabolism with non-alcoholic fatty liver disease ( NAFLD) .Methods Sixty SD rats were ran-domly divided into impaired glucose tolerance (IGT) model group (n=20), type 2 diabetes mellitus (T2DM) model group (n=20), IGT control group (n=10) and T2DM control group (n=10).IGT models were produced by feeding high-fat diet, T2DM models were produced by feeding high-fat diet for 4 weeks and intraperitoneally injected streptozotocin. Liver cell apoptosis was detected by TUNEL staining.The expression of MIF mRNA in liver tissue was determined by real-time PCR.The expressions of MIF, caspase-3, JNK proteins and phosphorylated JNK(p-JNK)in the liver tissue were de-termined by Western blotting.Results Apoptotic cells were obviously increased in the IGT and T2DM groups.Expression of MIF mRNA in the IGT and T2DM groups was markedly higher than that in the control group, respectively (P<0.01). The expressions of MIF, caspase-3, JNK proteins and phosphorylated JNK were markedly higher than that of the control group, respectively (P<0.05 or P<0.01).The expressions of MIF, caspase-3, JNK proteins in the T2DM group were significantly decreased compared with those of the IGT group, while the expression of phosphorylated JNK was significantly higher ( P<0.01) .Conclusions Abnormal glucose metabolism accompanied with NAFLD is probably related to the in-crease of MIF, Caspase-3, JNK and phosphorylated JNK expression.
7.Expression of perilipin and ADRP in rat liver tissues with abnormal glu-cose metabolism
Linhua FAN ; Maolin LIU ; Bingyan WEI ; Tianfu LIU ; Chunfang WANG ; Wenbiao PANG ; Yongchang GUO ; Jianxin LIU
Chinese Journal of Pathophysiology 2015;(3):534-538
[ ABSTRACT] AIM:To observe the changes of perilipin and adipose differentiation-related protein ( ADRP) du-ring the development of diabetes mellitus and to explore the effect of perilipin and ADRP on abnormal glucose metabolism with non-alcoholic fatty liver disease ( NAFLD) .METHODS:The rat model of impaired glucose tolerance ( IGT) was in-duced by feeding high-fat diet, and the type 2 diabetes mellitus (T2DM) model was induced by feeding high-fat diet for 4 weeks and intraperitoneally injecting streptozotocin.The morphological change of the liver tissue was observed under optical microscope.The serum contents of perilipin and ADRP were measured by ELISA.The mRNA expression of perilipin and ADRP in the liver tissues was detected by real-time PCR.The protein expression of ADRP in the liver tissues was deter-mined by Western blotting.RESULTS:HE staining showed steatosis in the liver of the rats in IGT group was more serious than that in T2DM group.The biochemical and the pathological processes of rat models were consistent with the clinical feature of related diseases.The serum content of perilipin had no difference among various groups.The mRNA expression of perilipin in IGT group and T2DM group was significantly higher than that in control group.Compared with IGT group, the mRNA expression of perilipin in T2DM group was significantly increased.The serum content of ADRP in T2DM group was significantly lower than that in control group.The mRNA and protein expression of ADRP in model groups was signifi-cantly lower than that in control group.Compared with IGT group, the mRNA expression of ADRP in T2DM group was sig-nificantly reduced.CONCLUSION: The serum content of ADRP plays a role in the development and progression of T2DM.It is negatively correlated with HOMA-IR.NAFLD occurs during progression of abnormal glucose metabolism in-duced by feeding high-fat diet.The development of abnormal glucose metabolism with NAFLD is probably related to the in-creased expression of perilipin and the reduced expression of ADRP.
8.Predictive value of cumulative body mass index on new-onset cholelithiasis
Tong LIU ; Yiming WANG ; Tianfu SI ; Wanchao WANG ; Liying CAO ; Siqing LIU
Chinese Journal of Digestive Surgery 2017;16(2):188-194
Objective To investigate the predictive value of cumulative body mass index (cumBMI) on new-onset cholelithiasis.Methods The retrospective cohort study was conducted.The data of 31 794 subjects who participated health examination at the Kailuan Hospital,Kailuan Linxi Hospital,Kailuan Zhaogezhuang Hospital,Kailuan Tangjiazhuang Hospital,Kailuan Fan'gezhuang Hospital,Kailuan Lyujiatuo Hospital,Kailuan Jinggezhuang Hospital,Kailuan Linnancang Hospital,Kailuan Qianjiaying Hospital,Kailuan Majiagou Hospital and Kailuan Branch Hospital in 2006,2008,2010,2012 and 2014 were collected.All the subjects were allocated into 4 groups according to squartiles of cumBMI:7 949 with cumBMI< 140.81 kg/m2 ×year in the Q1 group,7 946 with 140.81 kg/m2×year≤ cumBMI< 159.69 kg/m2 ×year in the Q2 group,7 949 with 159.69 kg/m2×year≤cumBMI< 180.49 kg/m2 ×year in the Q3 group and 7 950 with cumBMI ≥ 180.49 kg/m2×year in the Q4 group.All the subjects received respectively the five health examinations in 2006,2008,2010,2012 and 2014 at the same place.Epidemiological investigation,anthropometric parameters and biochemical indicators were collected.Observation indicators:(1) incidence of cholelithiasis in the 4 groups;(2) risk factors analysis affecting newonset cholelithiasis:sex,age,cumBMl,BMI,drinking,smoking,physical exercise,hypertension,diabetes,C-reactive protein (CRP),triglyceride (TG) and total cholesterol (TC).Measurement data with normal distribution were represented as-x±s and comparisons among groups were analyzed using the one-way ANOVA.Pairwise comparison and homogeneity of variance were done using the LSD test.Heterogeneity of variance was done using the Dunnett's T3 test.Measurement data with skewed distribution were described as M (Q) and comparisons among groups were analyzed using the nonparametric test.Count data were analyzed by the chi-square test.The incidence of cholelithiasis in the 4 groups were calculated by the Kaplan-Meier method and comparisons of incidence were done by the Log-rank test.The univariate analysis and multivariate analysis were done using the COX regression model.Results (1) Incidence of cholelithiasis in the 4 groups:31 794 subjects were observed for (2.1 ± 0.4) years,and 236 had new-onset cholelithiasis with an incidence of 7.42‰.Incidences of cholelithiasis in the Q1,Q2,Q3 and Q4 groups were respectively 4.03‰,7.17‰,7.93‰ and 10.57‰,with a statistically significant difference among the 4 groups (x2 =72.39,P<0.05).(2) Risk factors analysis affecting new-onset cholelithiasis:results of univariate analysis showed that sex,age,cumBMI,BMI,hypertension and CRP were independent risk factors affecting new-onset cholelithiasis of subjects [HR =1.61,1.75,1.64,1.36,1.39,1.39,95% confidence interval (CI):1.23-2.10,1.49-2.05,1.45-1.86,1.21-1.53,1.07-1.79,1.18-1.62,P<0.05].Results of multivariate analysis showed that female,age between 50 years and 60 years,age≥60 years,140.81 kg/m2×year ≤cumBMI <159.69 kg/m2×year,159.69 kg/m2×year≤cumBMI< 180.49 kg/m2 ×year,cumBMI ≥ 180.49 kg/m2 × year were independent risk factors affecting new-onset cholelithiasis of subjects (HR=1.59,1.78,2.33,2.04,2.42,3.66,95%CI:1.21-2.09,1.31-2.44,1.63-3.34,1.29-3.24,1.47-3.95,2.15-6.25,P<0.05).Conclusion Female,advanced age and increasing cumBMI are independent risk factors affecting new-onset cholelithiasis,and the incidence of cholelithiasis rises as cumBMI increases.
9.Study of mitochondrial genomes and molecular genetic marker of microsatellite in chinese hamster
Guohua SONG ; Zhaoyang CHEN ; Tianfu LIU ; Wenbiao PANG ; Jiping GAO ; Ruihu ZHANG ; Maolin LIU ; Linhua FAN
Chinese Journal of Comparative Medicine 2017;27(5):4-5,15
Chinese hamster is an important laboratory animal in medical and biological researches,but the molecular genetic markers research was rarely reported.In our study the base composition,gene structure,genetic evolution and other characteristics of mitochondrial genome of Chinese hamster were analyzed using the methods of bioinformatics and comparative genomics,genetic quality detection system of Chinese hamster were also established.These results would supply genome data for animal models of human diseases,and lay the foundation for scientific evaluation and reasonable utilization.
10.Curative effect of minimally invasive plate osteosynthesis for complex fractures of the distal femur
Guanglin WANG ; Gang WU ; Tianfu YANG ; Leiting CHI ; Yao FANG ; Lei LIU
Chinese Journal of Trauma 2008;24(9):715-717
Objective To evaluate the clinical results of minimally invasive plate osteosynthesis (MIPO) in treatment of complex fractures of the distal femur. Methads From July 2005 to December 2006, 41 patients with complex distal femur fractures induced by high energy trauma were treated with MIPO plus locking compression plate(LCP). There were 31 males and 10 females, at average age of 38 years(17-65 years). According to the AO/OTA fracture classification system, there were 11 patients with type A3 fractures, 22 with type C2 and 8 with type C3. There were 16 patients with open fractures. The clinical result was evaluated by using function examination and radiographic assessment. Results All patients were followed up for average 8 months(2-18 months), which showed fracture healing, with mean healing time of 13 weeks(7. 16 weeks). There was only 1 patient with superficial infection but no fixation failure or implant breakage were found. The average knee range of motion was 110°(45°-135°). Hospital for special surgery knee rating scale(HSS) showed excellent or good results in 36 patients (88%). No patient with greater than 5°difference in either varus or valgus was detected when compared 2 days after operation with latest follow-up. Conclusion In treatment of complex distal femur frac-tures, MIPO plus LCP have advantages of minor trauma, stable fLxation, slight interference on soft tis-sues, early fracture healing and few complications.