Objective: To observe the immediate effect of small-angle Tui-Pushing and An-Pressing anti-rotation bone-setting manipulation in improving the correction of braces for adolescent idiopathic scoliosis. Methods: A total of 50 cases of adolescent idiopathic scoliosis were selected and given brace correction first. The whole spine anteroposterior and lateral radiographs were taken, the Cobb angle was measured, and the visual analog scale (VAS) score of pain caused by brace wearing was recorded. After removal of the brace, small-angle Tui-Pushing and An-Pressing anti-rotation bone-setting manipulation was performed once. After treatment, the same brace was put on again to take a whole spine anteroposterior radiograph, the Cobb angle was measured, and the VAS score was recorded. The changes in Cobb angle and VAS score after manipulation were compared, and the immediate efficacy was evaluated. Results: After the manipulation, the Cobb angle was significantly smaller than that before treatment (P<0.01) and the VAS score was significantly lower than that before treatment (P<0.01). Conclusion: Small-angle Tui-Pushing and An-Pressing anti-rotation bone-setting manipulation can improve the immediate efficacy of brace in treating adolescent idiopathic scoliosis and relieve the pain caused by brace wearing at the same time.

Objective To evaluate the effect of different restoration methods on fracture resistance of endodontically treated teeth. Methods Fifty intact extracted maxillary first premolars were randomly divided into 5 groups. Medial-occlusal cavity models were established in all the test groups (B-E) according to the same standard, followed by treatments with defect exposure only, defect filling with light cured composite resin, indirect resin inlays, or light cured composite resin combined with Biosplint fiber. Each specimen was tested using a universal test machine at 1.00 mm/min until fracture and the fracture load was recorded. The load angle was 45 degree to the long axis of the teeth, and the load was pointed to the middle of the lingual surface on the buccal cusp. The fracture resistance was analyzed statistically. Results The mean load to cause fracture of the samples in each group group A to E was 1.27 ± 0.41, 0.23 ± 0.17, 0.55 ± 0.31, 0.89 ± 0.40, and 0.98 ± 0.34 kN, respectively, showing significant differences between the groups. Conclusion The fracture resistance of the teeth is reduced after endodontic therapy, but can be increased significantly by restoration with composite resin inlay or light cured composite resin combined with Biosplint fiber.

Objective To evaluate the effect of different restoration methods on fracture resistance of endodontically treated teeth. Methods Fifty intact extracted maxillary first premolars were randomly divided into 5 groups. Medial-occlusal cavity models were established in all the test groups (B-E) according to the same standard, followed by treatments with defect exposure only, defect filling with light cured composite resin, indirect resin inlays, or light cured composite resin combined with Biosplint fiber. Each specimen was tested using a universal test machine at 1.00 mm/min until fracture and the fracture load was recorded. The load angle was 45 degree to the long axis of the teeth, and the load was pointed to the middle of the lingual surface on the buccal cusp. The fracture resistance was analyzed statistically. Results The mean load to cause fracture of the samples in each group group A to E was 1.27 ± 0.41, 0.23 ± 0.17, 0.55 ± 0.31, 0.89 ± 0.40, and 0.98 ± 0.34 kN, respectively, showing significant differences between the groups. Conclusion The fracture resistance of the teeth is reduced after endodontic therapy, but can be increased significantly by restoration with composite resin inlay or light cured composite resin combined with Biosplint fiber.

OBJEVTIVETo evaluate the effect of different restoration methods on fracture resistance of endodontically treated teeth.

METHODSFifty intact extracted maxillary first premolars were randomly divided into 5 groups. Medial-occlusal cavity models were established in all the test groups (B-E) according to the same standard, followed by treatments with defect exposure only, defect filling with light cured composite resin, indirect resin inlays, or light cured composite resin combined with Biosplint fiber. Each specimen was tested using a universal test machine at 1.00 mm/min until fracture and the fracture load was recorded. The load angle was 45 degree to the long axis of the teeth, and the load was pointed to the middle of the lingual surface on the buccal cusp. The fracture resistance was analyzed statistically.

RESULTSThe mean load to cause fracture of the samples in each group group A to E was 1.27∓0.41, 0.23∓0.17, 0.55∓0.31, 0.89∓0.40, and 0.98∓0.34 kN, respectively, showing significant differences between the groups.

CONCLUSIONThe fracture resistance of the teeth is reduced after endodontic therapy, but can be increased significantly by restoration with composite resin inlay or light cured composite resin combined with Biosplint fiber.

Bicuspid ; physiopathology ; Composite Resins ; chemistry ; Dental Materials ; chemistry ; Dental Restoration, Permanent ; methods ; Dental Stress Analysis ; Humans ; Inlays ; Maxilla ; Tooth Fractures ; physiopathology ; Tooth, Nonvital ; physiopathology ; therapy

Objective To study the significance of leptin and its receptor(OB-R) in the occurrence and development of pulmonary arterial hypertension(PAH) induced by monocrotaline(MCT).Methods Fifteen SD rats were divided into the control group(n=5) and two experimental groups(n=10).The experimental groups were intraperitoneally injected by MCT for constructing the PAH model and the control group was injected by the same dose of solvent groups.The venous blood was extracted at 2,4 weeks after MCT injection in the two experimental groups.The mean pulmonary artery pressure(mPAP) and right ventricular hypertrophy index(RVHI) were measured and then the lung tissue was removed.The pathological change of lung blood vessels was observed.The expression of serum leptin was detected by ELISA.The expression of OB-R in lung tissue was tested by Western blot.Results Compared with the control group,mPAP and RVHI in the experimental groups were significantly increased(P＜0.05);the expression levels of serum leptin and lung tissue OB-R were increased significantly(P＜0.05),moreover,which were positively correlated with mPAP(r=0.912,P＜0.05;r=0.861,P＜0.05).Conclusion Leptin and OB-R may play an important role in the occurrence and development of PAH induced by MCT.

TNFR-Fc is an important fusion protein that has great potential in therapeutic and diagnostic applications. We developed an efficient fed-batch process for GS-CHO cells to produce TNFR-Fc. The rationale of this fed-batch process relies on the supply of sufficient nutrients to meet the requirements of cell metabolism. The optimal feed medium was designed through ration design. A metabolically responsive feeding strategy was designed and dynamically adjusted based on the residual glucose concentration determined off-line. In this process, the maximal viable cell density and antibody concentration reached above 9.4x10(6) cells/mL and 207 mg/L, respectively. Compared with the batch process, the newly developed fed-batch process increased the cell yield by 3.4 fold and the final antibody concentration by 3 fold. This fed-batch process would therefore facilitate the production of therapeutic antibody by GS-CHO cells.
Animals ; CHO Cells ; Cell Culture Techniques ; methods ; Cricetinae ; Cricetulus ; Culture Media ; Etanercept ; Glucose ; analysis ; Immunoglobulin G ; biosynthesis ; genetics ; Receptors, Tumor Necrosis Factor ; biosynthesis ; genetics ; Recombinant Fusion Proteins ; biosynthesis ; genetics

The velocity distribution,vortex evolution,viscous shear stress distribution and Reynolds shear stress distribution downstream of the aortic bileaflet mechanical heart valve are investigated under different physiological conditions using computational fluid dynamics method.At peak systole,the blood jet is more intense in the exercise state than in the other two states,with a maximum blood flow rate of 2.1 m/s.The analysis on vortex evolution shows that the shear layer is the obvious flow feature in the 3 physiological states and that the blood interacts more strongly with the aortic sinus at peak systole.For viscous shear stress,when the valve leaflets are fully open,the leaflets impede blood flow across the valve in the valvular region.The interaction between the blood flow and the valve leaflets results in high shear stress,and the maximum viscous shear stress observed in the 3 physiologic states is less than 8 N/m2.The maximum Reynolds shear stress observed in the 3 physiologic states is 700 N/m2.The study provides a theoretical basis for surgical valve selection and postoperative rehabilitation.

Objective To analyze the radiation exposure of medical personnel, and to provide real-time radiation dose monitoring data for medical personnel in interventional surgeries. Methods A total of 96 person-times who participated in cardiac surgeries in interventional operating rooms from September 2019 to March 2022 were selected as subjects. The subjects were divided into two groups according to the time progress, with 43 person-times in intervention group and 53 person-times in control group. The subjects in the intervention group were provided with radiation dose monitoring data during surgeries, while the subjects in the control group performed surgeries with routine procedures. The average intraoperative radiation dose rates of the two groups were compared. Results The median radiation dose rate was significantly lower in the intervention group than in the control group (88.80 μSv/h vs 188.40 μSv/h, U = 637.000, P = 0.000). Conclusion Providing real-time radiation dose monitoring data for medical personnel in interventional surgeries can effectively reduce their occupational radiation exposure and improve their occupational radiation protection.

Compared with the global average, the incidence rate of nasopharyngeal carcinoma (NPC) in China is higher, particularly in the southern regions where the mortality rate has remained persistently high. Nimotuzumab, a targeted therapy that acts on the epidermal growth factor receptor, has prompted continuous progress in NPC treatment. The combination of nimotuzumab with traditional radiotherapy and chemotherapy can enhance treatment efficacy, reduce adverse reactions, and improve patients’ quality of life. This article summarizes current research findings from this perspective to provide diagnostic and therapeutic strategies for NPC treatment.