Objective: To observe the immediate effect of small-angle Tui-Pushing and An-Pressing anti-rotation bone-setting manipulation in improving the correction of braces for adolescent idiopathic scoliosis. Methods: A total of 50 cases of adolescent idiopathic scoliosis were selected and given brace correction first. The whole spine anteroposterior and lateral radiographs were taken, the Cobb angle was measured, and the visual analog scale (VAS) score of pain caused by brace wearing was recorded. After removal of the brace, small-angle Tui-Pushing and An-Pressing anti-rotation bone-setting manipulation was performed once. After treatment, the same brace was put on again to take a whole spine anteroposterior radiograph, the Cobb angle was measured, and the VAS score was recorded. The changes in Cobb angle and VAS score after manipulation were compared, and the immediate efficacy was evaluated. Results: After the manipulation, the Cobb angle was significantly smaller than that before treatment (P<0.01) and the VAS score was significantly lower than that before treatment (P<0.01). Conclusion: Small-angle Tui-Pushing and An-Pressing anti-rotation bone-setting manipulation can improve the immediate efficacy of brace in treating adolescent idiopathic scoliosis and relieve the pain caused by brace wearing at the same time.

OBJECTIVE To invest igate the therapeutic effects of music combined cognitive behavioral therapy on subjective tinnitus and the changes in spectral characteristics of resting electroencephalogram(EEG) before and after treatment under eyes closed condition. METHODS 8 patients with subjective tinnitus were enrolled as experimental group including 4 males and 4 females, with a mean age of 33.57±8.52 years from 18 to 40 years, 6.00±3.63 months of illness and right-handedness. Music combined cognitive behavioral therapy included a total of 6 sessions of treatment. The initial session of the treatment aimed to establish basic trust with the patient and understand the patient's personality, tinnitus symptoms, medical history, and other basic conditions. The light music which was specially processed by mactching the frequency and intensity to the patient's tinnitus were selected as combined audio of cognitive behavioral therapy. 7 minutes of closed-eye resting state EEG, THI scale, VAS scale, and SAS scale of the patients with subjective tinnitus were collected. The last 5 sessions was a tailored relaxation therapy including training and counseling patients to understand the treatment feedback after each meeting. After 3 months of the therapy, 7 minutes of EEG, THI, VAS and SAS scales of the 8 patients were asessed. The results of 7min-EEG, THI, VAS, and SAS scales were compared in patients with tinnitus before and after treatment. In addition, 8 patients with normal hearing and without tinnitus were enrolled as control group including 4 males and 4 females, with a mean age of 21.26±7.56 years from 20 to 45 years and right-handedness. The control group underwent 7 minutes of EEG examination. RESULTS The difference between the two groups before treatment was statistically significant(t =4.404, P <0.05). The full-band(0.5-44 Hz) energy of EEG before and after treatment in 8 patients with subjective tinnitus was statistically significant(t =3.705, P <0.05), and the average power of EEG after treatment in the experimental group and the control group was similar. The THI scale(t =3.664, P <0.05) and VAS scale(t =3.265, P <0.05) were compared before and after treatment and the difference was statistically significant. The difference of SAS scales between the two groups was not statistically significant(P >0.05). CONCLUSION Music combined with cognitive behavioral therapy has a significant effect on subjective tinnitusand the closed-eye resting state of brain power can show the recovery of subjective tinnitus of patients objectively which has significance for rehabilitation.

Enzymes are widely used in chemical and pharmaceutical industries because of their advantages of high efficiency and specificity. However， the shortcomings of the free enzymes， such as poor stability and difficulty in recycling， limit their application. Therefore， the immobilization and application of enzymes have become one of the research hotspots. The selection of the immobilization carriers is a critical step in the process of enzyme immobilization. Metal-organic frameworks（MOFs）， a kind of porous materials， are formed by the coordination of metal ions or metal clusters with organic ligands. As an emerging immobilization carrier， its advantages such as high porosity， strong stability， and surface modifiability make it ideal for immobilized enzyme carriers. By immobilizing the free enzyme on MOFs， the above mentioned deficiencies of the free enzymes can be effectively solved， which greatly broaden the applicable condition. Ligand fishing is a method to find receptor-specific ligands from complex components， which has the advantages of high efficiency， simple sample pretreatment and high specificity. The MOF-enzyme complex formed by enzyme immobilization can act as a "fishing rod" for ligand fishing， which can screen out the targets from the complex system of components. The complex chemical composition and various active ingredients of traditional Chinese medicine（TCM） make the ligand fishing technology to play a big role in the screening of enzyme inhibitors from TCM. And the screened enzyme inhibitors are expected to be further developed into the lead compounds with good efficacy and low adverse effects， so the immobilized enzymes of MOFs have a wide application in the screening of active ingredients from TCM. Based on this， this paper summarized the methods of immobilized enzymes of MOFs in recent years， analyzed the characteristics， advantages and disadvantages of each method， and summarized the laws of preparation conditions and mechanisms. Meanwhile， the application and future development of immobilized enzymes of MOFs in the field of enzyme inhibitor screening from TCM were also summarized and prospected， with a view to providing a reference for the development of natural ingredients and the modernization of TCM.