Objective:To develop a Self-rating Scale for Scientific Research Ability of Nursing Staff and conduct reliability and validity test.Methods:Based on literature review, qualitative interview and expert letter consultation, the first draft of the Self-rating Scale for Scientific Research Ability of Nursing Staff was formed. By the convenient sampling method, a total of 924 nursing staff from Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology were selected as the research objects from February to March 2021 for questionnaire survey to test the reliability and validity of the scale.Results:Two common factors were extracted from exploratory factor analysis, including 13 items, and the cumulative variance contribution rate was 82.088%. Confirmatory factor analysis suggested that the model fit well. Content validity index at item level was 0.833~1.000, and content validity index at scale level was 0.865. The Cronbach's α coefficient of the total scale was 0.971, and the Cronbach's α coefficient of each dimension was 0.913 and 0.974. The total split-half reliability coefficient of the scale was 0.941, and split-half reliability coefficient of each dimension was 0.932 and 0.964.Conclusions:The self-rating scale for scientific research ability of nursing staff has good reliability and validity, which can be used as an evaluation tool of research ability of nursing staff.