At present,SARS-CoV-2 is still constantly evolving and mutating,with weakened pathogenicity but faster transmission,which still threatens human health. Anti-SARS-CoV-2 biotechnological drugs are still the hotspot of research and development in the field of biomedicine,and the effectiveness evaluation is very important for screening these drugs. Traditional effectiveness evaluation of anti-SARS-CoV-2 biotechnological drugs requires the live virus of SARS-CoV-2 that is difficult to obtain,and the requirement of high experimental conditions has limited the research and development of anti-SARS-CoV-2biotechnological drugs. SARS-CoV-2 pseudovirus can simulate the infectivity of live virus,but it has no infectivity of live virus,and is safe and easy to prepare,which is a favorable tool for evaluating the effectiveness of SARS-CoV-2 biotechnological drugs. In this paper,the epidemiological characteristics of SARS-CoV-2,the packaging system of SARS-CoV-2 pseudovirus and the application of SARS-CoV-2 pseudovirus in the effectiveness evaluation of biotechnological drugs were reviewed,so as to provide a reference for the application of SARS-CoV-2 pseudovirus in the development and quality evaluation of biotechnological drugs.

Objective To develop and validate an evaluation method based on Design of Experiment(DOE) for the relative activity of SARS-CoV-2 neutralizing antibody, so as to provide a reliable detection method for the development and quality evaluation of SARS-CoV-2 neutralizing antibody.Methods A method for evaluating the relative activity of SARS-CoV-2neutralizing antibody was developed by using SARS-CoV-2 variant pseudovirus. The cell density [(3-5) × 10~5cells/mL],pseudovirus titer(3 750-6 250 TCID_(50)/mL), neutralization time of antibody to pseudovirus(0. 5-1. 5 h), and interaction time of antibody-pseudovirus complex and cells(48-96 h) were optimized by DOE. The optimized method was verified for the specificity, linear range, accuracy and precision, and used to detect the relative activity of two batches of SARS-CoV-2neutralizing antibodies.Results The optimum cell density was 4 × 10~5cells/mL, the titer of pseudovirus was 6 250 TCID_(50)/mL,the neutralization time of antibody to pseudovirus was 1. 5 h, and the interaction time of antibody-pseudovirus complex and cells was 96 h. Only SARS-CoV-2 neutralizing antibody showed a typical dose-response curve, while anti-VEGF monoclonal antibody and sample diluent did not show a dose-response curve. The theoretical titer of SARS-CoV-2 neutralizing antibody in the range of 50%-200% showed a good linear relationship with the detection value, and the linear equation was y = 1. 034 2 x-0. 013,r = 0. 958 8. The relative bias of the mean values of the three test results of the samples at the relative titer levels of 50%, 71%, 100%, 141% and 200% ranged from 6. 2% to 7. 3%, and the geometric coefficient of variation(GCV)ranged from 3. 2% to 16. 9%. The relative activity of the two batches of SARS-CoV-2 neutralizing antibodies was 91% and118% respectively.Conclusion The developed method has good specificity, accuracy and precision, and can be used for the detection of the relative activity of SARS-CoV-2 neutralizing antibody.

Objective:To investigate the clinical characteristic of gastritis verrucosa,and improving the recognition and treatment of this disease thereof.Methods:Six hundred and fifty three patients with gastritis verrucosa,who were diagnosed in Tianjin general hospital from March 2003 to February 2009,were studied.All of these patients were examined by electronic gastroscope and conducted biopsy.Patients were detected helicobacter pylori(HP)using 13C urea breath test.Fifty eight patients with HP-positive and fifty patients with HP-negative(moderate and serious grade),were collected and divided into two groups respectively.The patients in HP-positive groups were given lanseprazole(group A)or rabeprazole(group B).Furazolidone and amoxicilin were given in both groups.The patients in HP-negative groups were administered lanseprazole(group C)or cimetidine(group D).The effects of the treatment were observed in this study.Results:There was no specificity in clinical characteristic of gastritis verrucosa.The pathological changes of gastritis verrucosa were mostly located antrum.The pathological manifestations were chronic inflammation and chronic active inflammation with intestinal metaplasia or atypical hyperplasia.The positive rate of HP infection was 72.74%.There was significant different in the relationship between different levels of inflammation and HP infection(P ＜ 0.05).The effect of treatment was better in group A than that of group B(P ＜ 0.05);and better in group C than that in group D(P ＜ 0.05).Conclusion:There was a correlation between gastritis verrucosa and HP infection.The higher positive rate of HP was correlated with the degree of erosion.For patients with HP-positive,it is better to use the treatment of lansoprazole,amoxicillin and furazolidone.For patients with HP-negative,treatment with lanseprazole was better than cimetidine.

Objective To investigate the influence of different concentrations of allicin in apoptosis of A549 cells induced by porphyromonas gingivalis (P.gingivalis). Methods The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of allicin in inhibiting P.gingivalis were investigated by broth dilution methods. The tetrazolium salts (MTT) assay was used to detect the viability of A549 cells infected by P.gingivalis and treated with different concentrations of allicin (64 mg/L, 96 mg/L and 128 mg/L). The flow cytometry FITC/PI staining was used to detect apoptotic rates of A549 cells treated by P.gingivalis and/or allicin for 24 h. Results The values of MIC and MBC of allicin for inhibiting P.gingivalis were 64 mg/L and 128 mg/L respectively. MTT assay showed that the cell viability was significantly increased with the increased concentration of allicin in a concentration-dependent manner (P < 0.05). There was no significant difference in cell viability between allicin (128 mg/L) group and control group, which showed that allicin was no significant cytotoxicity in A549 cells. Flow cytometry assay showed that the apoptotic rates from high to low were P. gingivalis group>P. gingivalis+allicin group>allicin group>control group with significant differences (P<0.01). The apoptosis of A549 cells induced by P.gingivalis was significantly inhibited by allicin (P<0.01). Conclusion Allicin can inhibit P.gingivalis infection in lung epithelial cells. There is a good prospect in the application of allicin in the treatment of pulmonary infection in patients with periodontitis.

OBJECTIVE:To investigate the feature and tendency of the application of antihypertensive drugs in our hospital.METHODS:The utilization of antihypertensive drugs in our hospital from Jun.2004 to Dec.2006 was analyzed statistically in respect of the consumption sum and DDDs etc.RESULTS:The first line drugs used most frequently in our hospital were beta receptor antagonists,AGT Ⅱ antagonists,ACEI,a calcium antagonist etc.Leading the first place on the list of DDDs was indapamide because of its good safety and efficacy.Mesylate amlodipine,felodipine,irbesartan,perindopril,metoprolol tartrate,indapamide,bisoprolol fumarate,losartan/hydrochlorothiazide ranked among the first 10 places in either DDDs or consumption sum.CONCLUSION:The antihypertensive drugs are characterized by complicated categorization,wide variety,and widespread clinical application,and the use of which in our hospital is basically rational.

ObjectivesTo investigate the effect of thyroid hormone on the expression of deiodinase and myelin basic protein in brain tissue of neonatal rats with germinal matrix-intraventricular hemorrhage (GM-IVH), and its nerve protective effect.Methods Sixty premature rabbits of gestational age 29 days were randomly divided into control, model and intervention groups. The prema-ture rabbits in model and intervention groups were given intraperitoneal injection of 50% glycerol to establish GM-IVH animal model and the premature rabbits in control group were given the same dose of saline. The GM-IVH was screened by cranial ultrasound. The premature rabbits with GM-IVH in intervention group were given intraperitoneal injection of triiodothyronine and the other two groups were given the same dose of saline. At 3 days, 7 days and 14 days after birth, the premature rabbits were killed and the brain tissue were quickly separated. The expression of type 2 iodothyronine deiodinase (D2) and type 3 iodothyronine deiodinase (D3) of periventricular zone tissue were detected by western blot method. The expression of myelin basic protein (MBP) were detected by immunohistochem-ical method.ResultsThe expression of D2 in brain tissue increased gradually in three groups. At the same time point, the expression of D2 was the lowest in the model group, the highest in the control group, and the middle in the intervention group. The differences of D2 among three groups were statistically signiifcant (allP<0.001). The expression of D3 increased gradually in the control and model groups, while it was the highest in the intervention group at day 7. At the same time point, the expression of D3 in the control group was the lowest, it was higher in intervention group than that in the model group at 3 days, then it was higher in the model group than that in the intervention group. The differences of D3 among three groups were statistically signiifcant (allP<0.001). The expression of MBP increased gradually in the control and intervention groups, and it decreased gradually in the model group. At the same time point, the MBP was the lowest in the model group, it was the highest in the control group, and it was the middle in the intervention group. The differences of MBP among three groups were statistically signiifcant (P<0.001).ConclusionsThe expressions of D2 and MBP in the brain tissues of premature rabbit with the GM-IVH increased while the expression of D3 decreased in some extent after thyroxine intervention.

T-2 toxin is a secondary fungal metabolite that belongs to the trichothecene mycotoxin family,it is the most toxic in class A trichothecene mycotoxin.T-2 toxin can induce apoptosis in cells bearing high proliferating activity.The mycotoxin readily passes the placenta and is distributed to embryo tissues,which results in fetal brain damage,bone malformation,thymic atrophy and even death.This article reviewed the effects of T-2 toxin on immune system,blood system and cell toxicities in pregnant mice and generation mice.

A method to assess library-holding journals according to their weighted citations was proposed in light of the actual needs of users for core journals, the use of library-holding journals and references by authors in different orders of precedence.The Western journal of rheumatology, highly cited by authors of Second Military Medical U-niversity, were assessed using this method, showing that this method is better than the citation analysis-based tra-ditional method in assessment of journals.

Objective: To study the biological characteristics of tumor cells infected with recombinant adenovirus expressing hTNF-?, investigate the antitumor effect of recombinant adenovirus. Methods: Human lung adenocarcinoma cell line Anip973 was infected with recombinant adenovirus expressing hTNF-?. Cell growth assay, colone formation test, flowcytometry assay and morphology were used to observe the effects on tumor cells. The hTNF-a gene, which was transduced into cancer cells mediated by recombinant adenovirus, was detected by PCR and agarose gel electrophoresis and its products were detected by ELISA assay. The intratumoral injection of rAd-LacZ and rAd-hTNF-? was carried out to evaluate their antitumor effects. Results: The liter of rAd reached 1010 PFU/ml and more than 90% Anip973 cells could be infected by 30MOI rAd. Except the surface structure and ultrastructure of tumor cells infected with rAd had a light change, cell growth abillity assay, colone formation test, flow cytometry assay showed no significant difference compared with that of the control cells. The TNF-? gene expression at 24 h increased greatly. Antitumor study indicated that on the tumor-bearing mice treated with rAd the tumor grew slowly. Tumor volume was significantly smaller and survive time was prolonged than that of controls. Conclusion: There was no significantly changes occurred on tumoral cells after infected with recombinant adenovirus expressing hTNF-?. The intratumoral injection of rAd-LacZ and rAd-hTNF-? could inhibit the growth of solid tumor.

Objective To investigate the possibility and accuracy of predicting malignant middle cerebral artery infarction (mMCAI) with bedside electroencephalography (EEG). Methods Thirty-five patients with massive hemispheric infarction (MHI) underwent bedside EEG monitoring within 48 h of onset. The EEG indicators were interpreted blindly, and the clinical, laboratory and imaging parameters were analyzed. The patients were divided into mMCAI group and non-mMCAI group according to whether they had occurred mMCAI or not within 7 days of onset. The differences of EEG indicators, clinical, laboratory and imaging parameters between the 2 groups were compared. When the parameters of significant difference and statistical significance appeared the odds ratio (OR) of occurring mMCAI were analyzed, and their accuracy of predicting mMCAI was calculated. Results Of the 35 patients with MHI, 20 were in the mMCAI group and 15 were in the non-mMCAI group. There were significant differences in the EEG indicators (infarction on the contralateral side, including disintegration of occipital α rhythm, generalized slow-wave, dominant frequency wave low amplitude, regional attenuation without delta [RAWOD]pattern, and absence of EEG reactivity), clinical parameters (nausea accompanied with vomiting), and imaging parameters (the infracted area more than the entire MCA territory, and midline shifting 3 to 5 mm at the level of septum pellucidum) between the 2 groups (P < 0. 05). Of those, the risk of mMCAI was the highest in patients with disintegration of occipital a rhythm on the contralateral side of infarction (P = 22. 67, 95% CI 3. 89-132. 10). The sensitivity of predicting mMCAI was 85. 0%, the specificity was 80.0%, the positive predictive value was 85.0%, and the negative predictive value was 80. 0%, which were superior to other EEG indicators and clinical or imaging parameters. Conclusions Bedside EEG indicators can early predict mMCAI, moreover, the predictive accuracy is superior to the clinical and imaging parameters.