2.Assessment of abstracts on randomized controlled trials in non-small cell lung cancer published in Chinese
Pan ZHANG ; Xia QIU ; Juan HE ; Long GE ; Cong MA ; Zhanjun MA ; Lei MAO ; Jinhui TIAN
Chinese Journal of Medical Library and Information Science 2016;(2):75-80
Objective To assess the abstracts on randomized controlled trials ( RCT) in non-small cell lung cancer ( NSCLC) published in Chinese and their influencing factors.Methods RCT in NSCLC published in Chinese were included according to the CONSORT statement and their influencing factors were analyzed by RevMan 5.3 soft-ware.Results The titles were identified as random, randomization, blinding, statistical method, recruited partici-pants, trial registry and fund-supported, respectively, in 20%of the 2677 abstracts included in this study.Con-clusion The titles are identified as random, randomization, blinding, statistical method, recruited participants, trial registry and fund-supported in RCT published in Chinese.Although the abstracts are improved after the publication of CONSORT, they need to be further brushed up.
3.Reconstruction of caprine mandibular segmental defect by tissue engineered bone reinforced by titanium reticulum.
Qing XI ; Rong-Fa BU ; Hong-Chen LIU ; Tian-Qiu MAO
Chinese Journal of Traumatology 2006;9(2):67-71
OBJECTIVETo investigate the feasibility of using natural poritos as scaffolds in bone tissue engineering (TE) and repair of caprine mandibular segmental defect with titanium reticulum reinforced.
METHODSNatural poritos with a pore of 190-230 microm in size and porosity of about 50percent-65percent was molded into the shape of granules 5 mm x 5 mm x 5 mm in size. Expanded autologous caprine marrow mesenchymal stem cells were induced by recombinant human morphogenetic protein-2 (rhBMP2) to improve osteoblastic phenotype. Then marrow derived osteoblasts were seeded into poritos in density of 4 x 10(7)/ml and incubated in vitro for 48 hours prior to implantation. Then osteoblastic cells/poritos complexes were implanted into mandibular defect and the defect was reinforced by titanium reticulum. Implantation of poritos alone acted as the control. Bone regeneration was assessed 4, 8, 16 weeks after implantation using roentgenographic analysis and histological observation was done after 16 weeks.
RESULTSNew bone could be observed histologically on the surface and in the pores of natural coral in all specimens in the cell-seeding group, whereas in the control group there was no evidence of osteogenesis process in the center of the construction. The results showed that new bone grafts were successfully restored 16 weeks after implantation.
CONCLUSIONSThis study suggests the feasibility of using porous coral as scaffold material transplanted with marrow derived osteoblasts by TE method. By means of titanium reticulum reinforcement, mandibular defect could be successfully restored. It shows the potentiality of using this method for the reconstruction of bone defect in clinic.
Animals ; Anthozoa ; Bone Marrow Cells ; Bone Morphogenetic Proteins ; Cell Culture Techniques ; Chondrogenesis ; Goats ; Mandible ; diagnostic imaging ; pathology ; surgery ; Mice ; Osteoblasts ; transplantation ; Osteogenesis ; Porosity ; Radiography ; Reconstructive Surgical Procedures ; Stents ; Tissue Engineering ; Titanium
4.Network meta-analysis on selecting Chinese medical injections in radiotherapy for esophageal cancer.
Long GE ; Lei MAO ; Jin-hui TIAN ; Fang-yu SHI ; Lou LI-LI ; Xia QIU ; Jin-long LI ; Ke-hu YANG
China Journal of Chinese Materia Medica 2015;40(18):3674-3681
To assess the clinical effect and safety of Chinese traditional medicine injection combined with radiotherapy for esophageal cancer. The relative randomized controlled trials (RCTs) of Chinese medical injections (CMI) combined with radiotherapy as well as simple radiotherapy for esophageal cancer were searched from PubMed, Cochrane Library, EMBASE, Chinese Biomedical Literature Database(CBM), China National Knowledge Infrastructure (CNKI), Wanfang Database and VIP Database as at September 2014. Two researchers completed the data extraction and quality evaluation independently. The data were analyzed by GeMTC 0.14.3 and Stata 12. 0 software. Finally, 43 RCTs involving 3 289 patients were finally included. The star network was constructed by different comparison groups. The results of network meta-analysis showed that the seven CMIs combined with radiotherapy was superior to simple radiotherapy in the treatment of esophageal cancer in efficacy, quality of life, and reduction in the incidence of nausea and leucopenia, but with no significant difference among the seven CMIs. Probability ranking result showed a great possibility for Shenqi Fuzheng and astragalus polysaccharide injections in improving the overall response rate and quality of life, which were followed by cinobufagin and kangai injections. However, only one study was included for Shenqi Fuzheng and astragalus polysaccharide injections. Therefore, cinobufagin or kangai injections were preferred in improving the overall response rate and quality of life. Aidi or compound sophora injections were better than other CMIs in reducing? the incidences of nausea (III-IV) and leukopenia. More RCTs of Shenqi Fuzheng and astragalus polysaccharide injections combined with radiotherapy for patients with esophageal cancer were expected in the future to confirm our results. Moreover, study findings will be reported, particularly for the adverse events in radiotherapy for esophageal cancer.
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Child
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Combined Modality Therapy
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Drugs, Chinese Herbal
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administration & dosage
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Esophageal Neoplasms
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drug therapy
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radiotherapy
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Female
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Humans
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Male
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Middle Aged
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Young Adult
5. The axillary vein puncture can reduce the incidence of central venous catheter-related bloodstream infection
Liquan HUANG ; Tian QIU ; Zizhuo LIU ; Shi Hao MAO ; Zheqi CHEN ; Rong Lin JIANG
Chinese Journal of Emergency Medicine 2019;28(10):1305-1308
Objective:
To compare the effects of various interventions on the incidence of central line-associated bloodstream infection (CLABSI) .
Methods:
The clinical data of 218 patients with central venous catheterization were retrospectively analyzed. Infected patients were treated as CLABSI group and non-infected patients as control group.
Results:
Of the 218 patients, 24 patients were developed CLABSI. There was no significant difference in sex, age, primary infection status and puncture site between CLABSI group and control group. Univariate analysis showed that axillary vein puncture could significantly reduce the incidence of CLABSI (
6.Constructing tissue engineered trachea-like cartilage graft in vitro by using bone marrow stromal cells sheet and PLGA internal support: experimental study in bioreactor.
Jun-rui ZHANG ; Fu-lin CHEN ; Wei WU ; Jian-hua WEI ; Xing-hua FENG ; Tian-qiu MAO
Chinese Journal of Plastic Surgery 2009;25(2):124-128
OBJECTIVETo explore the feasibility of constructing tissue engineered trachea-like cartilage graft in vitro by using bone marrow stromal cells (BMSCs) sheet and PLGA internal support.
METHODSRabbit BMSCs were expanded and induced by transforming growth factor-1 to improve chondrocyte phenotype of BMSCs. BMSCs sheets were obtained by continuous culture and wrapped the PGLA scaffold in the shape of cylinder. The constructs were incubated in spinner flask for 8 weeks and cartilage formation was investigated by gross inspection, histology, glycosaminoglycan and mechanical strength content.
RESULTSAfter in vitro culture, cartilage like tissue in cylindrical shape had been regenerated successfully. Stiff, shiny, pearly opalescence tissues were observed. Histological analysis showed engineered trachea cartilage consisted of evenly spaced lacunae embedded in matrix, cells stationed in the lacunae could be noticed clearly. Safranin-O staining on the sections showed homogenous and positive red staining, which demonstrated that the engineered tissue was rich in proteoglycans.
CONCLUSIONSBased on the cell sheet and internal support strategy, trachea-like cartilage in cylindrical shape could be successfully fabricated which provided a highly effective cartilage graft substitute and could be useful in many situations of trachea-cartilage loss encountered in clinical practice.
Animals ; Biocompatible Materials ; Bone Marrow Cells ; cytology ; Cartilage ; Feasibility Studies ; Female ; Lactic Acid ; Male ; Polyglycolic Acid ; Rabbits ; Stromal Cells ; cytology ; Tissue Engineering ; methods ; Tissue Scaffolds ; Trachea ; surgery
7.Current status of cerebral glioma surgery in China.
Jin-song WU ; Jie ZHANG ; Dong-xiao ZHUANG ; Cheng-jun YAO ; Tian-ming QIU ; Jun-feng LU ; Feng-ping ZHU ; Ying MAO ; Liang-fu ZHOU
Chinese Medical Journal 2011;124(17):2569-2577
The treatment of gliomas is highly individualized. Surgery for gliomas is essentially for histological diagnosis, to alleviate mass effect, and most importantly, to favor longer survival expectancy. During the past two decades, many surgical techniques and adjuvants have been applied to glioma surgery in China, which lead to a rapid development in the field of cerebral glioma surgery. This article broadly and critically reviewed the existing studies on cerebral glioma surgery and to portrait the current status of glioma surgery in China. A literature search was conducted covering major innovative surgical techniques and adjuvants for glioma surgery in China. The following databases were searched: the Pubmed (January 1995 to date); China Knowledge Resource Integrated Database (January 1995 to date) and VIP Database for Chinese Technical Periodicals (January 1995 to date). A selection criterion was established to exclude duplicates and irrelevant studies. The outcome measures were extracted from included studies. A total of 3307 articles were initially searched. After excluded by abstracts and full texts, 69 studies conducted in the mainland of China were included and went through further analysis. The philosophy of surgical strategies for cerebral gliomas in China is undergoing tremendous change. Nowadays Chinese neurosurgeons pay more attention to the postoperative neurofunctional status of the patients. The aim of the glioma surgery is not only the more extensive tumor resection but also the maximal safety of intervention. The well balance of longer overall survival and higher quality of life should be judged with respect to each individual patient.
China
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Glioma
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pathology
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surgery
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Humans
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Magnetic Resonance Imaging
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Neurosurgery
8.A study of injectable autogenous tissue-engineered bone.
Wei-dong YANG ; Qiang CAO ; Qi-chun QIAN ; Li-hui TANG ; Lian-jia YANG ; Tian-qiu MAO
Chinese Journal of Stomatology 2003;38(5):393-395
OBJECTIVETo investigate the utilization of carrier for delivering osteoblasts and creating autogenous bone tissue in ectopic site of animal via injection.
METHODSBone marrow cells harvested from iliac bone of New Zealand rabbits were induced to differentiate into marrow stromal osteoblasts. The osteoblasts were mixed with 1.5% alginate sodium solution to generate osteoblasts/alginate composites with final cellular density of 4 x 10(9)/L. Calcium chloride was used as cross-linking agent to gel aqueous alginate solution. The marrow stromal osteoblasts/alginate composites were injected into the dorsal subcutaneous tissue of rabbits with autogenous cells transplantation. The samples were examined with X-ray and histological analysis.
RESULTSFour, eight and twelve weeks after injection, the hard knobbles were easily palpated under the dorsal skin of animals. On X-ray photograph the samples showed calcified image with more density than surrounding soft tissue, new bone formation was observed in the osteoblasts/alginate composites in histological analysis. The osteogenesis was in association with regenerated hematopoietic bone marrow.
CONCLUSIONSThese results demonstrate that new bone tissue could be created through the injection of alginate sodium treated with autogenous marrow stromal osteoblasts.
Alginates ; administration & dosage ; Animals ; Glucuronic Acid ; administration & dosage ; Hexuronic Acids ; administration & dosage ; Osteoblasts ; transplantation ; Osteogenesis ; Rabbits ; Tissue Engineering
9.Single-stage posterior spondylectomy, circumferential decompression and reconstruction using mesh cage for spinal tumors.
Yong ZHU ; Hong ZHAO ; Giu-Xing QIU ; Jian-Guo ZHANG ; Ye TIAN ; Shu-Gang LI ; Suo-Mao YUAN
Chinese Medical Sciences Journal 2009;24(3):172-177
OBJECTIVETo explore the feasibility and clinical results of circumferential decompression and three-column reconstruction through single-stage posterior transpedicular approach for spinal tumor treatment.
METHODSTotally, 24 patients with spinal tumor underwent tumor resection and spinal reconstruction through single-stage posterior transpedicular approach. Preoperatively, according to the Frankel classification, 12 patients were grade E, 9 grade D, and 3 grade C. Anterior column was reconstructed with non-expandable titanium cages. Posterior segmental instrumentation was used to maintain the stability of spine in all cases. Anterior and posterolateral fusion was performed with autograft and allogenic bone. The following data were followed up in these patients: deformity angle, local recurrence, neurological function, and spinal bony fusion.
RESULTSThe average operating time and blood loss was 5.6 hours and 3,400 ml respectively. No intraoperative and postoperative complications were observed in this group. Postoperatively, 21 patients were Frankel grade E, 2 grade D, and 1 grade C. Four patients reported significant functional restoration and twenty patients reported complete resolution of pain. At follow-up (range, 6-42 months), implant failure or recurrent neurological symptoms was not found.
CONCLUSIONSThe tumor resection and spinal reconstruction through single-stage posterior transpedicular approach is a safe and effective technique for the treatment of spinal tumor. It can fully decompress the neurological structures, correct the kyphosis, and achieve early weight-bearing. This technique can improve life quality for the patients with spinal tumor.
Adult ; Aged ; Decompression, Surgical ; methods ; Female ; Humans ; Laminectomy ; methods ; Male ; Middle Aged ; Orthopedic Procedures ; methods ; Reconstructive Surgical Procedures ; methods ; Spinal Neoplasms ; surgery ; Surgical Mesh ; Young Adult
10.Repair of peripheral nerve defect by a scroll of amnion derivative compound with cultured autogenous Schwann cell in a rat model.
Qi ZHANG ; Xiao-ming GU ; Guang-yan YU ; Tian-qiu MAO ; Jing-chen ZHENG ; Qing-ying TONG
Chinese Journal of Stomatology 2006;41(2):98-101
OBJECTIVETo test a nerve bridge substitute for peripheral nerve repair by tissue-engineering approach.
METHODSAn artificial nerve fabricated with a scroll of amnion derivative (ZQ membrane) and cultured autogenous Schwann cell was sutured to bridge sciatic nerve defect of 2.5 cm in length in rats. The specimens were assessed with tracking study, histology, electrophysiological technique, NF200, and synaptophysin-38 (SYP) immuno histochemical staining 3 months postoperatively.
RESULTSThe regenerated nerve sprouted 3 months after the operation. The regenerated nerve fibers were plentiful and could grow into the recipient nerve and target muscle's motor end plate (MEP) areas to reinnervate target muscle, and reconstruct function of nerve-muscle junction. Functional recovery could reach to 40%-60% of normal control. Nerve-muscle conduction velocity (N-MCV) arrived at 21.77 +/- 1.15 m/s.
CONCLUSIONSA tissue engineering material fabricated with a scroll of ZQ membrane and cultured autologous Schwann cell may be a useful substitute for nerve repair.
Amnion ; cytology ; Animals ; Cells, Cultured ; Female ; Male ; Nerve Regeneration ; physiology ; Rats ; Rats, Sprague-Dawley ; Schwann Cells ; cytology ; Sciatic Nerve ; injuries ; surgery ; Tissue Engineering ; methods