1.Variation in STAT4 is associated with systemic lupus erythematosus in Chinese Northern Han population.
Yin SU ; Yi ZHAO ; Xu LIU ; Jian-Ping GUO ; Quan JIANG ; Xiang-Yuan LIU ; Feng-Chun ZHANG ; Yi ZHENG ; Xiao-Xia LI ; Hui SONG ; Ci-Bo HUANG ; Yan-Hong HUANG ; Tian WANG ; Si-Si PAN ; Chun LI ; Xia LIU ; Lei ZHU ; Chun-Fang ZHANG ; Zhan-Guo LI
Chinese Medical Journal 2010;123(22):3173-3177
BACKGROUNDRecent studies have identified signal transducer and activator of transcription 4 (STAT4) as a susceptibility gene for systemic lupus erythematosus (SLE) in different populations. In order to examine whether the allele distribution of the single nucleotide polymorphism (SNP) in gene STAT4 rs7574865 in patients with SLE is different from those of healthy controls in Chinese Northern Han population, we investigated whether the variants of STAT4 rs7574865 were associated with any specific clinical features of SLE.
METHODSWe genotyped SNPs in STAT4 rs7574865 in 252 patients with SLE and 497 healthy controls. All subjects were from the Northern part of Chinese Han population. The genotypes in rs7574865 were determined by polymerase chain reaction (PCR) and consequence direct sequencing of PCR products in the DNA samples.
RESULTSThere was a significant difference in distribution of the SNPs in rs7574865 between the SLE patients and healthy controls. Compared with healthy controls, there was a significant correlation between TT genotypes in rs7574865 and the risk of SLE when GG genotype was used as a reference genotype after adjusting for gender and age. The frequency of T allele in the SLE patients was strongly significantly higher than that of healthy controls. Furthermore, there was a significant difference in the distribution of SNP in rs7574865 between male and female SLE patients, when compared with healthy controls. The frequency of T allele in rs7574865 in male patients was significantly higher than that of male healthy controls or female patients. There was no significant correlation between the frequencies of T allele in STAT4 rs7574865 and the clinical features of SLE.
CONCLUSIONSThe SNP rs7574865 in STAT4 is strongly associated with risk of SLE in the Chinese Northern Han population. The TT genotype and T allele in STAT4 rs7574869 are susceptibility factors for SLE, especially for male SLE patients.
Adult ; Asian Continental Ancestry Group ; genetics ; Female ; Genetic Predisposition to Disease ; genetics ; Genotype ; Humans ; Linkage Disequilibrium ; genetics ; Lupus Erythematosus, Systemic ; genetics ; Male ; Middle Aged ; Polymorphism, Single Nucleotide ; genetics ; STAT4 Transcription Factor ; genetics ; Young Adult
2.Mantle cell lymphoma: clinicopathologic features and prognostic factors of 102 cases occurring in Chinese patients.
Hong JI ; Gan-di LI ; Feng-yuan LI ; Yan-qiong BAI ; Yu CHEN ; Ming-zhong YANG ; Lian-jun WANG ; Yan TANG ; Pei ZHANG ; Tian XIA ; Ci LI ; Jiang FENG ; Zong-kai ZOU ; Jia-cuo YIXI
Chinese Journal of Pathology 2007;36(11):730-735
OBJECTIVETo study the clinicopathologic features and prognostic factors of Chinese patients with mantle cell lymphoma.
METHODSOne hundred and two cases of mantle cell lymphoma occurring in Chinese patients were studied by light microscopy and immunohistochemistry. The follow-up information was also analyzed. The cases were classified as mantle zone, nodular or diffuse patterns and as typical or blastoid variants. Age, Ann-Arbor staging, B symptoms, hematologic parameters, histologic variants, mitotic index and immunophenotype were assessed for possible prognostic implication.
RESULTSThe median age of the patients was 59 years (range: 30 to 79 years) and the male-to-female ratio was 2.92:1. Seventy-one patients (87.65%) presented with advanced stage disease (Ann Arbor stage III to IV). B symptoms were present in 45.45% of patients. The commonest site of involvement was lymph node (100%). The other involved sites included bone marrow (64.44%), spleen (63.16%), Waldeyer's ring (31.25%), peripheral blood (29.41%), liver (22.64%) and gastrointestinal tract (14.71%). All cases expressed B-cell markers but were negative for T-cell marker. Majority of cases were positive for cyclin D1 (94.12%) and CD5 (71.43%). Blastoid variant accounted for 24.51% of cases. Amongst the 68 cases with follow-up data available, the median survival was 10 months. Parameters associated with shorter survival included diffuse pattern, blastoid variant, high mitotic index, high proliferative activity and presence of bone marrow involvement.
CONCLUSIONSThe clinicopathologic features and prognostic factors of mantle cell lymphoma occurring in Chinese are similar to those in Caucasians. Diffuse pattern, blastoid variant, high mitotic index, high proliferative activity and involvement of bone marrow indicate poor prognosis.
Adult ; Aged ; Antigens, CD20 ; metabolism ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; CD5 Antigens ; metabolism ; CD79 Antigens ; metabolism ; Combined Modality Therapy ; Cyclin D1 ; metabolism ; Cyclophosphamide ; therapeutic use ; Doxorubicin ; therapeutic use ; Female ; Follow-Up Studies ; Humans ; Lymphoma, Mantle-Cell ; metabolism ; pathology ; therapy ; Male ; Middle Aged ; Prednisone ; therapeutic use ; Prognosis ; Vincristine ; therapeutic use
3.Study on the integrated monitoring program regarding mouse and main mouse-borne disease in Zhejiang province
Zhen-Yu GONG ; Qi-Yong LIU ; Juan HOU ; Gui-Ming FU ; Rong-Fu CHEN ; Jin-Bao LEI ; Zhi-Ping CHEN ; Tian-Ci YANG ; Liang LU ; Zhang-Yao REN ; Yu-Qing LAN ; Zhong-Bing CHEN ; Yu-Hong GUO ; Feng LING ; Ji-Min SUN
Chinese Journal of Epidemiology 2011;32(5):494-498
Objective To study the integrated monitoring program regarding mouse and plague, hemorrhagic fever of renal syndrome(HFRS)and leptospirosis. Methods Integrated monitoring plan was used. A designated office coordinated 5 departments' actions within the Zhejiang Provincial Center for Disease Control and Prevention(CDC). Cage-trapping method was conducted to monitor the density of mice from June to October, respectively. Results Lishui municipal CDC had finished the integrated monitoring program on mouse and mouse-borne disease while the Longyou CDC had finished the field investigation, using the integrated monitoring program.Specimens were sent to provincial CDC. The integrated monitoring program needed more number of personnel and better coordination. Lishui reported 3 leptospirosis cases and 58 HFRS cases in 2009,with the incidence rates as 0.13 and 2.44 per 100 000, respectively. Longyou reported 2 leptospirosis case and 1 HFRS cases in 2009, with the incidence rates as 0.49 and 0.25 per 100 000, respectively.Lishui and Longyou had no plague case. Lishui caught 91 mice in 2009 and the density was 4.17%.Longyou caught 37 mice in 2009, with the density as 1.18 percent. Most mice caught from Lishui were Apodemus agrarius and the next was Mus musculus. In Longyou the Rattus tanezumi ranked the first, followed by Apodemus agrarius. The positive rate of HFRS antigen in Lishui and Longyou were 10.42% and 4.59% respectively. The positive rate of HFRS antibody in Longyou was 3.70%. The culture positive rate of leptospirosis in mouse renal of Lishui and Longyou were 0 and 0.98%respectively. The culture positive rate of leptospirosis in pig renal, duck renal, frog renal and cattle urine of Longyou was 0. The culture positive rate of leptospirosis in duck blood of Longyou was 80%.Conclusion The integrated monitoring program on mouse and mouse-borne disease seemed to be feasible and could promote the integrated surveillance and control program on mouse and mouse-borne diseases in China.
4.Simultaneous Bilateral Pulmonary Resections by Uniportal Video-assisted Thoracic Surgery for Bilateral Multiple Pulmonary Nodules
Guang-wen XU ; Ming-ran XIE ; Gao-xiang WANG ; Tian-ci ZHANG ; Jie-yong TIAN ; Tian LI
Journal of Sun Yat-sen University(Medical Sciences) 2022;43(6):1012-1019
ObjectiveTo explore the application of simultaneous bilateral uniportal video-assisted thoracic surgery in the treatment of bilateral multiple pulmonary nodules. MethodsThe clinical and pathological characteristics , and perioperative data were analyzed in thoracic surgery from August 2021 to August 2021 at Department of Thoracic Surgery, the First Affiliated Hospital of University of Science and Technology of China. During the study period, 40 patients were included in the study, of which 12 were male, 28 were female, the average age was (52±8.8) [52.5(47.25~58)] years. ResultsA total of 107 nodules were resected, with 85 malignancy nodules, including 30 patients with bilateral primary lung cancer, 6 patients with primary lung cancer on one side. All patients underwent bilateral uniportal video-assisted thoracoscopic surgery (Uni-portal VATS), the average intraoperative blood loss was (48.87±19.29) [40(30~67.5)] mL, the average operation time was (147.70±54.24) [145(113.5~170)] min, the average number of resected lymph nodes was (6.20±9.13) [3(0~12)],the average NRS score in the 1th postoperative day was (5.08±2.23), the average pleural drainage was (375.95±243.69) [292.5(215.0~517.5)] mL. the average thoracic drainage time was(3.38±1.49) [3(3~4)] days, and the average postoperative hospital stay was (4.08±1.81) [4(3~5)] days. Postoperative complications including: 2 cases of infection, 3 cases of atrial fibrillation, 1 case of hemoptysis for more than 1 week and 1case of persistent air leakage for more than 3 days. All of them improved after treatment, and there were no serious complications and deaths in perioperative period. ConclusionSimultaneous bilateral pulmonary resections via uniportal VATS is a safe and feasible minimally invasive procedure for patients with bilateral multiple pulmonary nodules.
5.Clinical Efficacy of Chonglian Oral Liquid on COVID-19 Patients
Tong ZHOU ; Xi'an ZHANG ; Yuli TIAN ; Huimin CI ; Xinru WANG ; Caiyun ZHU ; Zegeng LI ; Jiabing TONG
Chinese Journal of Experimental Traditional Medical Formulae 2023;29(17):142-149
ObjectiveTo observe the effect of Chonglian oral liquid on inflammatory and immune markers as well as the clinical outcomes of patients with mild-to-moderate corona virus disease 2019(COVID-19) and comprehensively evaluate its efficacy and safety. MethodA clinical randomized controlled trial (RCT) was conducted, involving 120 confirmed cases of mild-to-moderate COVID-19. The patients were randomly divided into two groups, with 55 cases in the observation group and 56 cases in the control group. According to the updated diagnosis and treatment protocol, the control group received standard western medical treatment, while the observation group received Chonglian oral liquid in addition to standard western medical treatment. Both groups were treated continuously for 10 days. The traditional Chinese medicine (TCM) syndrome scores, syndrome efficacy, fever abatement time, nucleic acid negative conversion time, inflammatory and immune markers, improvement in imaging findings, clinical outcomes, and occurrence of adverse events were compared between the two groups. ResultBoth groups showed a significant decrease in TCM syndrome scores after treatment (P<0.01). Compared with the control group after treatment, the observation group exhibited a more significant improvement in cough, dry throat, sore throat, fatigue, and muscle pain (P<0.05). The total effective rate in the observation group was 100% (55/55), significantly higher than 98.21% (55/56) in the control group (Z=3.707, P<0.01). The observation group also showed a significantly shorter duration of fever abatement and nucleic acid negative conversion compared with the control group after treatment (P<0.05). Both groups had a significant increase in lymphocyte count (LYM), lymphocyte percentage (LYM%), mature T lymphocytes (CD3+), and helper/inducer T lymphocytes (CD4+) after treatment (P<0.01). Compared with the control group after treatment, the observation group showed greater improvement in these markers (P<0.05). In terms of inflammatory markers, both groups had a significant decrease compared with those before treatment (P<0.01). The observation group exhibited lower levels of high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), and procalcitonin (PCT) than the control group after treatment (P<0.05). There was no statistically significant difference in imaging efficacy evaluation and clinical outcomes between the two groups. No adverse events were reported in either group during the treatment period. ConclusionChonglian oral liquid combined with standard western medical treatment significantly improves clinical symptoms, shortens fever abatement and nucleic acid negative conversion time, regulates immune function, and inhibits inflammatory responses in patients with mild-to-moderate COVID-19, leading to improved clinical efficacy.
6.Short Term Complications and Safety Analysis of Retrosternal Reconstruction Pathway Combined with Postoperative Adjuvant Radiotherapy in Minimally Invasive Mckeown Esophagectomy
Jie-yong TIAN ; Xin-yu MEI ; Tian-ci ZHANG ; Guang-wen XU ; Ran XIONG
Journal of Sun Yat-sen University(Medical Sciences) 2022;43(4):653-660
ObjectiveTo compare and analyze the effect of tubular gastric transsternal route and posterior mediastinal route combined with postoperative adjuvant radiotherapy on the short-term complications and safety of minimally invasive Mckeown esophageal carcinoma resection. MethodsRetrospective analysis of 89 patients who underwent minimally invasive Mckeown esophagectomy in the Department of thoracic surgery of the First Affiliated Hospital of China University of science and technology from January 2020 to June 2021. Some patients received postoperative adjuvant radiotherapy. According to the tubular gastric lifting pathway, they were divided into two groups: retrosternal route group and posterior mediastinal route group, including 39 cases in the retrosternal route group and 50 cases in the posterior mediastinal route group. The clinical characteristics and perioperative complications of the two groups were compared. ResultsThere was no significant difference in clinical characteristics between the two groups (P>0.05); there was no significant difference in operation time, intraoperative bleeding, postoperative drainage and postoperative hospital stay between the two groups (P>0.05). In addition, the incidence of pulmonary infection in the posterior mediastinal pathway group was higher than that in the retrosternal pathway group, and the difference was not statistically significant (P>0.05). The incidence of gastroesophageal reflux in the posterior mediastinal route group was higher than that in the retrosternal route group, and the difference was statistically significant (P<0.05). A total of 30 patients received adjuvant radiotherapy (10 cases in the retrosternal route group and 20 cases in the posterior mediastinal route group). The incidence of radioactive gastritis in the retrosternal route group was lower than that in the posterior mediastinal route group (1 case had acute radioactive esophagitis, grade 1; In the posterior mediastinal route group, 6 patients developed acute radiation esophagitis (gastritis), there were 2 cases in grade 1 and 4 cases in grade 2). ConclusionThe short-term efficacy and safety of tubular gastric retrosternal route and posterior mediastinal route in minimally invasive Mckeown esophagectomy are consistent, but the incidence of gastroesophageal reflux after retrosternal route is lower.
8.Effect of active component compound of Epimedii Folium,Astragali Radix,and Puerariae Lobatae Radix on expression of ADAM17 in HT22 cells by mediating hepcidin.
Xian-Hui DONG ; Xiao-Ping HE ; Tian-Ci ZHANG ; Dong-Xue MA ; Jia-Qi LI ; Xiao-Xiao LIU ; Hao LI ; Wei-Juan GAO
China Journal of Chinese Materia Medica 2021;46(23):6224-6230
Alzheimer's disease(AD) patients in China have been surging, and the resultant medical burden and care demand have a huge impact on the development of individuals, families, and the society. The active component compound of Epimedii Folium, Astragali Radix, and Puerariae Lobatae Radix(YHG) can regulate the expression of iron metabolism-related proteins to inhibit brain iron overload and relieve hypofunction of central nervous system in AD patients. Hepcidin is an important target regulating iron metabolism. This study investigated the effect of YHG on the expression of a disintegrin and metalloprotease-17(ADAM17), a key enzyme in the hydrolysis of β amyloid precursor protein(APP) in HT22 cells, by mediating hepcidin. To be specific, HT22 cells were cultured in vitro, followed by liposome-mediated siRNA transfection to silence the expression of hepcidin. Real-time PCR and Western blot were performed to examine the silencing result and the effect of YHG on hepcidin in AD cell model. HT22 cells were randomized into 7 groups: control group, Aβ25-35 induction(Aβ) group, hepcidin-siRNA(siRNA) group, Aβ25-35 + hepcidin-siRNA(Aβ + siRNA) group, Aβ25-35+YHG(Aβ+YHG) group, hepcidin-siRNA+YHG(siRNA+YHG) group, Aβ25-35+hepcidin-siRNA+YHG(Aβ+siRNA+YHG) group. The expression of ADAM17 mRNA in cells was detected by real-time PCR, and the expression of ADAM17 protein by immunofluorescence and Western blot. Immunofluorescence showed that the ADAM17 protein expression was lower in the Aβ group, siRNA group, and Aβ+siRNA group than in the control group(P<0.05) and the expression was lower in the Aβ+siRNA group(P<0.05) and higher in the Aβ+YHG group(P<0.05) than in the Aβ group. Moreover, the ADAM17 protein expression was lower in the Aβ+siRNA group(P<0.05) and higher in the siRNA+YHG group(P< 0.05) than in the siRNA group. The expression was higher in the Aβ+siRNA+YHG group than in the Aβ+siRNA group(P<0.05). The results of Western blot and real-time PCR were consistent with those of immunofluorescence. The experiment showed that YHG induced hepcidin to up-regulate the expression of ADAM17 in AD cell model and promote the activation of non-starch metabolic pathways, which might be the internal mechanism of YHG in preventing and treating AD.
ADAM17 Protein
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Alzheimer Disease/genetics*
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Amyloid beta-Peptides
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Drugs, Chinese Herbal/pharmacology*
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Hepcidins/genetics*
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Humans
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Pueraria
9.Association between brain glucose metabolism and cardiac dysfunction in patients with ischemic heart disease undergoing (18)F-FDG PET/CT imaging.
Xia LU ; Bin Bin NIE ; Ming Kai YUN ; Zi Wei ZHU ; Xiao Fen XIE ; Tian Tian MOU ; Hong Zhi MI ; Yong Xiang WEI ; Xiang LI ; Bao Ci SHAN ; Xiao Li ZHANG
Chinese Journal of Cardiology 2020;48(3):211-216
Objective: To evaluate the relationship between the brain glucose metabolism and left ventricular function parameters, and to explore the cerebral glucose metabolism reduction regions in patients with ischemic heart disease (IHD). Methods: A total of 110 consecutive IHD patients who underwent gated (99)Tc(m)-sestamibi (MIBI) SPECT/CT myocardial perfusion imaging, gated (18)F-fluorodeoxyglucose (FDG) PET/CT myocardial and brain glucose metabolic imaging within three days in Beijing Anzhen Hospital from April 2016 to October 2017, were enrolled in this study. Left ventricular functional parameters of SPECT/CT and PET/CT including end-diastolic volume (EDV), end-systolic volume (ESV) and left ventricular ejection fraction (LVEF) were analyzed by QGS software. Viable myocardium and myocardial infarction region were determined by 17-segment and 5 score system, and the ratio of viable myocardium and scar myocardium was calculated. According to the range of viable myocardium, the patients were divided into viable myocardium<10% group (n=44), viable myocardium 10%-<20% group (n=36) and viable myocardium≥20% group (n=30). Pearson correlation analysis was used to analyze the correlation between the range of viable myocardium and scar myocardium and the level of cerebral glucose metabolism. Brain glucose metabolism determined by the mean of standardized uptake value (SUV(mean)) was analyzed by SPM. The ratio of SUV(mean) in whole brain and SUV(mean) in cerebellum were calculated, namely taget/background ratio (TBR). Differences in cerebral glucose metabolism among various groups were analyzed by SPM. Results: There were 101 males, and age was (57±10) years in this cohort. The extent of viable myocardium and the extent of scar, LVEF evaluated by SPECT/CT and PET/CT were significantly correlated with TBR (r=0.280, r=-0.329, r=0.188, r=0.215 respectively,all P<0.05). TBR value was significantly lower in viable myocardium<10% group, compared with viable myocardium 10%-<20% group (1.25±0.97 vs. 1.32±0.17, P<0.05) and viable myocardium≥20% group (1.25±0.97 vs. 1.34±0.16, P<0.05). Furthermore, in comparison with viable myocardium≥20% group, the hypo-metabolic regions of viable myocardium<10% group were located in the precuneus, frontal lobe, postcentral gyrus, parietal lobe, temporal lobe, and so on. Conclusions: There is a correlation between impaired left ventricular function and brain glucose metabolism in IHD patients. In IHD patients with low myocardial viability, the level of glucose metabolism in the whole brain is decreased, especially in the brain functional areas related to cognitive function.
Aged
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Brain
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Fluorodeoxyglucose F18
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Glucose
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Humans
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Male
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Middle Aged
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Positron Emission Tomography Computed Tomography
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Radiopharmaceuticals
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Stroke Volume
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Tomography, Emission-Computed, Single-Photon
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Ventricular Function, Left
10.Prevalence and risk factors of Giardia lamblia infections among colorectal cancer patients in Henan Province
Hui-hui CHEN ; Yan DENG ; Zhi LI ; Zhen-lei WANG ; Zeng-ci RUN ; Ting ZHANG ; Yu-chun CAI ; Hong-wei ZHANG ; Zhu-hua HU ; Jun-hu CHEN ; Li-guang TIAN ; Jian LI
Chinese Journal of Schistosomiasis Control 2022;34(4):370-377
Objective To investigate the epidemiological characteristics and identify the risk factors of Giardia lamblia infections among patients with colorectal cancer in Henan Province. Methods A cross-sectional study was performed for questionnaire surveys among colorectal cancer patients in Henan Cancer Hospital during the period from March to July, 2021. Patients’ stool samples were collected, and the triosephosphate isomerase (tpi) gene of G. lamblia was amplified in stool samples using nested PCR assay to characterize the parasite genotype. Univariate analysis and multivariate logistic regression analyses were employed to identify the risk factors of G. lamblia infections among colorectal cancer patients. Results A total of 307 colorectal cancer patients were investigated, including 176 males (57.3%) and 131 females (42.7%). PCR assay detected 8.1% [95% confidential interval (CI): (0.056, 0.117)] prevalence of G. lamblia infections among the study subjects, and there was no significant difference in the prevalence between men [9.1%, 95% CI: (0.057, 0.143)] and women [6.9%, 95% CI: (0.037, 0.125)] (χ2 = 0.495, P = 0.482). In addition, there was no age-specific prevalence of G. lamblia infections among the participants (χ2 = 1.534, P = 0.675). Multivariate logistic regression analysis identified use of septic tanks [odds ratio (OR) = 3.336, 95% CI: (1.201, 9.267)], daily use of well water [OR = 3.042, 95% CI: (1.093, 8.465)] and raising livestock [OR = 3.740, 95% CI: (1.154, 12.121)] as risk factors of G. lamblia infections among colorectal cancer patients, and the prevalence of abdominal pain was significantly greater in colorectal cancer patients with G. lamblia infections than in those without infections (P = 0.017). Among the 25 patients with G. lamblia infections, assemblage A was characterized in 24 (96.0%) cases and assemblage B in one case (4.0%). Conclusions The prevalence of G. lamblia is high among colorectal cancer patients in Henan Province, and assemblage A is the dominant genotype of G. lamblia. Use of septic tanks, daily use of well water and raising livestock are risk factors of G. lamblia infections among patients with colorectal cancer.