Objective To evaluate the recent impact and long-term effects of recombinant human acidic fibroblast growth factor (rh-aFGF) of cesarean section on women's abdominal incision healing.Methods In 516 cases of cesarean section in women,randomly divided into placebo group (172 cases),spray group (172 cases) and flush group (172 cases).The placebo group received 0.9％ sodium chloride washing abdominal incision suture.The spray group incision suture direcdy after the administration of rh-aFGF spray,per square meter of 3-4 sprays,3-5 times/d.The flush group was given before 2 rh-aFGF dissolved in 10-20 ml of 0.9％ sodium chloride directly after washing the incision suture,then given spray treatment.The recent follow-up of 15 d observed maternal abdominal incision healing.Long term follow-up of 3 months to observe the incision scar formation.Results The abdominal incision heating time was (8.6 ±3.5) d,that was obviously shorter than that in spray group (10.4 ±3.7) d and placebo group (12.7 ± 4.9) d (P ＜ 0.05).The delay healing accounted for 4.2％ (7/166) in flush group,significantly lower than that in placebo group (34/168,20.2％) and spray group (16/165,9.7％) (P＜ 0.05).There was no significant difference in the incidence of fat liquefaction,postoperative infection among three groups (P ＞ 0.05).Flush group exudation,abdominal wall adhesions was lower than placebo group and spray group [2.4％(4/166) vs.10.1％(17/168),5.5％(9/165),3.6％(6/166) vs.17.3％(29/168),9.7％(16/165)](P＜0.05).Long term follow-up,flush group scar area than spray group reduced by 13.5％,than placebo group reduced by 27.2％.Conclusions A recent rh-aFGF can reduce the cesarean section abdominal incision healing time and reduce abdominal wall adhesions and exudation;rh-aFGF the best dosage is intraoperative irrigation treatment spray combined with postoperative.

Objective To investigate the concentration changes of vascular endothelial growth factor(VEGF), nitric oxide(NO) and endothelin (ET) in elderly patients with obstructive sleep apnea syndrome (OSAS) combined with eerebrovascular disease.Methods One hundred and thirty-two subjects were divided into four groups: OSAS with cerebrovaseular disease group (OC group), OSAS without cerebrovascular disease group (O group), cerebrovascular disease without OSAS group (C group) and normal control group (N group).The concentrations of VEGF, NO and ET in the plasma were compared in each subgroup.Results The concentration of VEGF was significantly increased in OC group and O group compared with C group and N group[(195.34±56.7)ng/L, (162.34±48.7)ng/L, (156.4 4±51.8) ng/L, (114.1 ± 54.2) ng/L, F= 21.02, P< 0.05].The concentration of NO was increased gradually in OC group, C group, O group and N group[(62.3±4.9)mmol/L,(64.7±5.1) mmol/L, (66.2± 4.2)retool/L, (77.5 ± 6.8) mmol/L, F= 17.35, P<0.05], and the concentration of ET was decreased gradually in OC group, C group, O group and N group[(59.8±9.6)ng/L, (56.5±4.3)ng/L, (54.7±7.9)ng/L, (37.2±8.5)ng/L, F= 4.27, P<0.05].Conclusions The concentrations of VEGF and ET are increased and the concentration of NO is decreased in patients with OSAS.Patients with OSAS combined with cerebrovascular disease have higher VEGF and ET concentrations and lower NO concentration than in patients with simple OSAS.The results indicate that vascular endothelial dysfunction may play an important role in the development of cerebrovascular disease in patients with OSAS.

Objective To evaluate the effect of music and mood relaxation training for the anxiety in patients with breast tumor before operation. Methods With case-contrast method, 108 patients with breast tumor (52 patients with intervention, 56 with routine treatment) week assessed continuously. All cases were investigated on their common information, physiological indexes and psychological scales, including State-Trait Anxiety Inventory (STAI-Form Y), Social Support Rating Scale (SSRS) and Eysenck Personality Questionaire (EPQ). Results There were significant statistical difference in systolic pressure and pulse rate between two groups on one day (t =3.114, P ＜0.01) and 30-60 minutes (t =3.03, P=0.01) before the operation. There was remarkable difference for anxiety between two groups and among different interventions periods by Repeated Measures ANOVA analysis (Periods: F=18.61, P ＜0.01; Groups: F =7.001, P ＜0.05). Results of Stepwise regression analysis: The influencing factors on anxiety in patients with breast tumor before operation were trait anxiety, breast-feeding history, family history of breast tumor, age, economy and EPQ-N. Conclusion Relaxation music and mood relaxation training can reduce the level of anxiety and related physiological indexes on patients with breast tumor before operation. The influencing factors on anxiety in patients with breast tumor before operation are multi level factors, such as trait anxiety, breast-feeding history and so on.

The completion of sequencing human genome creates a new era of biological science and technology. Although the sequence of the human genome has been known, it is still hard to rapidly explore the whole functional genes, especially, their interaction with each other and the meaning to the body. However, the "reverse biology" which comes into being in the recent years provides us a series of novel ideas and technologies for discovering new functional gene, among which the immune-related genes have attracted more attentions, clarifying how functional gene works and their potential value in application.

insufficiency of both the spleen and the kidney (type E). The serum levels of ALT and AST in type B were significantly more higher than the other syndromes (P0.05). Conclusion The distribution of TCM types of syndrome of the patients with HBeAg-negative chronic hepatitis B has its characteristic. There are some relationships between TCM types of syndrome and the levels of transaminases, HBVDNA, the abnormal rates of four hepatic fibrosis markers.

The social reputation-based incentive mechanisms in preventing the free riding behaviors were put for-ward according to the analysis of personal digital library and P2P technology. Since the reputation value of personal digital library was calculated according to the maximum flow algorithm, personal digital library must increase its reputation value by sharing the resources when it is going to obtain the necessary resources it wants.

[Objeetive]To report the repairing offect of cancellous bone defect with calcium sulphate.[Method]A defect 5mm in diameter and 12 mm in deep,which was drilled in the lateral aspect of rabbit lateral femoral condyle,was filled with CCaS(Citrated Calcium Sulphate)cylinder or Osteoset tablets respectively.Rabbits were killed 3,6,13 weeks after surgery.And then general oberservations and mechanical analyzing were conducted.[Result]After surgery,rabbits had good movements,spirits and eating,good healing of incision.There was no arthroedema and inflammatory response around the joints.Mechanical analyzing reports:both anticompressive strength and elasticity coefficient in the artificial bone repair bone defect area 3 weeks after surgery,were higher than in that of normal femur condyle;Anti-compressive strength and elasticity coefficient in the defect area 6 weeks,which drop comparing to 3 weeks,was lower than that of normal femur condyle;Anti-compressive strength and elasticity coefficient 13 weeks,which is higher than 6 weeks,is approaching normal.Mechanics intensity of two groups in the different time had no significant difference,but was significantly higher than one of the same time blank group.[Conclusion]To some extent,Calcium Sulphate has mechanical supportive effects on cancelloues bone defect in spite of the absorption and degradation of Calcium Sulphate.There is no significant difference between Citrated Calcium Sulphate and Osteoset tablets.

Objective To construct small RNAi plasmid vectors which can suppress the expression of TITIN in Wistar rat. Methods A recombinant plasmid vector involving shRNA, which matched the base pair of rat TITIN N2B region mRNA perfectly, was constructed and transfected into normal neonatal cardiomyocytes and mesenchymal stem cells (MSCs) induced by 5-aza cytidine respectively. The expression of TITIN Z band was investigated by immunofluorescence. Results The expression of TITIN was weakened after recombinant plasmid was transfected into neonatal cardiomyocytes and MSCs induced by 5-aza cytidine. Conclusion The recombinant plasmid was constructed successfully, and it actually can block the expression of TITIN.

Objective To adopt a three-dimensional limited element analytic method to research into the cause and mechanism of the complications taking place after resection of head radius.Method To adopt a three-dimensional limited element analytic method to research into the stress transmission action of the head radi-us and the distribution alteration of the stress transmission after resection of head radius impacting on the stability of elbow and wrist.Result In normal cases,the stress value transmitted via head of radius takes 58% of the stress placed,36.85% via humeroulnar joint.The head of radius places a leading role in the stress transmission of the elbow joint.After rasection of the head of radius,the lateral of humeroulnar joint bears about 43% of the stress placed while the medial part bears about 21.6% of the stress.To compare with that before the resection of the head of radius the stress on the lateral is obviously increased more than the medial part.Conclusions The head of radius places a leading role in the stress transm ission of the el-bow joint.The resection of the head radius breaks the normal distribution of the stress on the elbow joint and produces great effect on the stability of the elbow joint and wrist joint.Therefore,in clinics we should pay great attention to the indication of rasection of the head of radius.

OBJECTIVE:To establish the method for preparation of compound fluconazole eye drops and to determine the content of fluconazole in the preparation METHODS:Detection was performed by second order derivative spectrophotometry RESULTS:Linear relationship was found in the fluconazole concentrations ranging from 80?g/ml to 400?g/ml The regression equation was D=0 0 094+0 002C,r=0 9 998 The recovery of fluconazole was 99 83%(RSD=0 69%,n=5) CO_NCLUSION:The eye drops was stable The method of determination of the content is simple,rapid and reliable