Objective To evaluate the recent impact and long-term effects of recombinant human acidic fibroblast growth factor (rh-aFGF) of cesarean section on women's abdominal incision healing.Methods In 516 cases of cesarean section in women,randomly divided into placebo group (172 cases),spray group (172 cases) and flush group (172 cases).The placebo group received 0.9％ sodium chloride washing abdominal incision suture.The spray group incision suture direcdy after the administration of rh-aFGF spray,per square meter of 3-4 sprays,3-5 times/d.The flush group was given before 2 rh-aFGF dissolved in 10-20 ml of 0.9％ sodium chloride directly after washing the incision suture,then given spray treatment.The recent follow-up of 15 d observed maternal abdominal incision healing.Long term follow-up of 3 months to observe the incision scar formation.Results The abdominal incision heating time was (8.6 ±3.5) d,that was obviously shorter than that in spray group (10.4 ±3.7) d and placebo group (12.7 ± 4.9) d (P ＜ 0.05).The delay healing accounted for 4.2％ (7/166) in flush group,significantly lower than that in placebo group (34/168,20.2％) and spray group (16/165,9.7％) (P＜ 0.05).There was no significant difference in the incidence of fat liquefaction,postoperative infection among three groups (P ＞ 0.05).Flush group exudation,abdominal wall adhesions was lower than placebo group and spray group [2.4％(4/166) vs.10.1％(17/168),5.5％(9/165),3.6％(6/166) vs.17.3％(29/168),9.7％(16/165)](P＜0.05).Long term follow-up,flush group scar area than spray group reduced by 13.5％,than placebo group reduced by 27.2％.Conclusions A recent rh-aFGF can reduce the cesarean section abdominal incision healing time and reduce abdominal wall adhesions and exudation;rh-aFGF the best dosage is intraoperative irrigation treatment spray combined with postoperative.

Objective: To evaluate the effect of RBa-I, an extract of Rosa bracteata Wend1, against withdrawa1 syndrome in morphine-dependent rats and to explore its mechanism. Methods: The mode1 rats of morphine dependent were established. The scores of withdrawa1 syndrome,?-EP level in hypotha1amus and pituitary body were observed to evaluate the effect of RBa-I in rats. Results: RBa-I can antagonize the urging withdrawa1 syndrome in morphine-dependent rats (ora1ly, 5 mg?kg -1 ), and the effect was inferior to chlonidine (oral1y, 0.2 mg?kg -1 ). It can a1so antagonize the natural withdrawal syndrome, decrease the tota1 scores of withdrawa1 syndrome and inhibit the decreasing of body weight. After a seven-day treatment course, the tota1 scores of withdrawa1 syndrome were decreased remarkab1y. The content of ?-EP in hypotha1amus and pituitary body were increased. Conclusion: RBa-I can antagonize the withdrawa1 syndrome of morphine-dependent rats and this may be related to the increase of ?-EP level in hypothalamus and pituitary body in rats.

The completion of sequencing human genome creates a new era of biological science and technology. Although the sequence of the human genome has been known, it is still hard to rapidly explore the whole functional genes, especially, their interaction with each other and the meaning to the body. However, the "reverse biology" which comes into being in the recent years provides us a series of novel ideas and technologies for discovering new functional gene, among which the immune-related genes have attracted more attentions, clarifying how functional gene works and their potential value in application.

Objective To investigate the effects of tea polyphenol on oligodendrocyte(OLG) in the cerebral ischemic rats. Methods Male Sprague Dawley rats were randomly divided into sham-operated group, control group and tea polyphenol group. The models of whole-brain ischemia were established . Then the rats of tea polyphenol group were divided another 3 groups and injected intraperitoneally with tea polyphenol at diffenrent dose (25 mg/kg, 50 mg/kg, 100 mg/kg, respectively) once a day for seven days . The expressions of precursor OLG cells, pedo-OLG cells and mature OLG cells in the cortex were examined by immunohistochemistry and the positive cells were counted.Results Compared with sham-operated group, the number of precursor OLG cells increased and the number of pedo-OLG cells and mature OLG cells decreased significantly in the control group (all P

[Objeetive]To report the repairing offect of cancellous bone defect with calcium sulphate.[Method]A defect 5mm in diameter and 12 mm in deep,which was drilled in the lateral aspect of rabbit lateral femoral condyle,was filled with CCaS(Citrated Calcium Sulphate)cylinder or Osteoset tablets respectively.Rabbits were killed 3,6,13 weeks after surgery.And then general oberservations and mechanical analyzing were conducted.[Result]After surgery,rabbits had good movements,spirits and eating,good healing of incision.There was no arthroedema and inflammatory response around the joints.Mechanical analyzing reports:both anticompressive strength and elasticity coefficient in the artificial bone repair bone defect area 3 weeks after surgery,were higher than in that of normal femur condyle;Anti-compressive strength and elasticity coefficient in the defect area 6 weeks,which drop comparing to 3 weeks,was lower than that of normal femur condyle;Anti-compressive strength and elasticity coefficient 13 weeks,which is higher than 6 weeks,is approaching normal.Mechanics intensity of two groups in the different time had no significant difference,but was significantly higher than one of the same time blank group.[Conclusion]To some extent,Calcium Sulphate has mechanical supportive effects on cancelloues bone defect in spite of the absorption and degradation of Calcium Sulphate.There is no significant difference between Citrated Calcium Sulphate and Osteoset tablets.

Objective To give a dose reference to the researcher who study prevention of restenosis using endovascular brachytherapy Methods The dose distributions around a 3 mm diam by 20 mm long balloon filled with 90 Y , 32 P and 186 Re separately have been calculated by a computer program based on a dose point kernel Results The peak value of the absorbed dose is found inside the balloon, the inflexion of the dose curve at the endothelial surface of the vessel In the vessel tissue the dose decreases with distance in an approximately exponential manner The lower the nuclide energy is, the faster the dose falls with distance, and the longer the region of uniform dose distribution along vessel wall is The dose rate at the surface of the vessel wall is approximately 74, 71, and 45 mGy/min per mCi/ml, with the dose rate decreasing to 53%, 48% and 28% at 0 5 mm for 90 Y , 32 P and 186 Re separately Conclusion The fast falling of the dose in an approximately exponential manner in the vessel tissue makes for focusing ? radionuclide energy to the vessel wall target and reducing the injury of the normal tissue around the vessel, and especially 186 Re with lower energy has a more obvious advantage in this aspect

Objective To construct small RNAi plasmid vectors which can suppress the expression of TITIN in Wistar rat. Methods A recombinant plasmid vector involving shRNA, which matched the base pair of rat TITIN N2B region mRNA perfectly, was constructed and transfected into normal neonatal cardiomyocytes and mesenchymal stem cells (MSCs) induced by 5-aza cytidine respectively. The expression of TITIN Z band was investigated by immunofluorescence. Results The expression of TITIN was weakened after recombinant plasmid was transfected into neonatal cardiomyocytes and MSCs induced by 5-aza cytidine. Conclusion The recombinant plasmid was constructed successfully, and it actually can block the expression of TITIN.

Objective To evaluate the effect of music and mood relaxation training for the anxiety in patients with breast tumor before operation. Methods With case-contrast method, 108 patients with breast tumor (52 patients with intervention, 56 with routine treatment) week assessed continuously. All cases were investigated on their common information, physiological indexes and psychological scales, including State-Trait Anxiety Inventory (STAI-Form Y), Social Support Rating Scale (SSRS) and Eysenck Personality Questionaire (EPQ). Results There were significant statistical difference in systolic pressure and pulse rate between two groups on one day (t =3.114, P ＜0.01) and 30-60 minutes (t =3.03, P=0.01) before the operation. There was remarkable difference for anxiety between two groups and among different interventions periods by Repeated Measures ANOVA analysis (Periods: F=18.61, P ＜0.01; Groups: F =7.001, P ＜0.05). Results of Stepwise regression analysis: The influencing factors on anxiety in patients with breast tumor before operation were trait anxiety, breast-feeding history, family history of breast tumor, age, economy and EPQ-N. Conclusion Relaxation music and mood relaxation training can reduce the level of anxiety and related physiological indexes on patients with breast tumor before operation. The influencing factors on anxiety in patients with breast tumor before operation are multi level factors, such as trait anxiety, breast-feeding history and so on.

OBJECTIVE:To establish the method for preparation of compound fluconazole eye drops and to determine the content of fluconazole in the preparation METHODS:Detection was performed by second order derivative spectrophotometry RESULTS:Linear relationship was found in the fluconazole concentrations ranging from 80?g/ml to 400?g/ml The regression equation was D=0 0 094+0 002C,r=0 9 998 The recovery of fluconazole was 99 83%(RSD=0 69%,n=5) CO_NCLUSION:The eye drops was stable The method of determination of the content is simple,rapid and reliable

Objective To adopt a three-dimensional limited element analytic method to research into the cause and mechanism of the complications taking place after resection of head radius.Method To adopt a three-dimensional limited element analytic method to research into the stress transmission action of the head radi-us and the distribution alteration of the stress transmission after resection of head radius impacting on the stability of elbow and wrist.Result In normal cases,the stress value transmitted via head of radius takes 58% of the stress placed,36.85% via humeroulnar joint.The head of radius places a leading role in the stress transmission of the elbow joint.After rasection of the head of radius,the lateral of humeroulnar joint bears about 43% of the stress placed while the medial part bears about 21.6% of the stress.To compare with that before the resection of the head of radius the stress on the lateral is obviously increased more than the medial part.Conclusions The head of radius places a leading role in the stress transm ission of the el-bow joint.The resection of the head radius breaks the normal distribution of the stress on the elbow joint and produces great effect on the stability of the elbow joint and wrist joint.Therefore,in clinics we should pay great attention to the indication of rasection of the head of radius.