Objective To investigate the value of ultrasound in diagnosis of fetal heart malformation at 11-13+6 weeks gestation.Methods A total of 70 pregnant women were selected,based on the results of induction of labor and postnatal imaging examination, the effect of ultrasound on fetal heart abnormalities in 11-13+6 weeks was observed.The display of cardiac malformations with different cuts was compared.Results In 70 fetuses with cardiac malformations, malformation types included tetralogy of Fallot, double outlet right ventricle, complete endocardial cushion defect, transposition of the great arteries, persistent truncus arteriosus, aortic arch, venous drainage.Ultrasound diagnosis of 64 cases of fetal cardiac malformations, the diagnostic accuracy rate was 91.4%.Among them, the highest proportion of tetralogy of Fallot (42.9%), venous drainage was the lowest (2.9%).The diagnostic rate of 4CV+3VVT ultrasound for fetal heart malformation was the highest(91.4%), which was significantly higher than 4CV+VOTV(72.2%), 4CV+(AR-SAV)(68.6%) and 4CV, the differences were statistically significant (x2=7.643,5.324,4.447,all P<0.05).The diagnostic accuracy of 4CV+3VV ultrasound for fetal heart malformation was 88.6%, which was significantly higher than 4CV+ (AR-SAV) and 4CV section, the differences were statistically significant (x2=6.343,4.011,all P<0.05).Conclusion Ultrasound can be used to detect fetal heart malformation at 11-13+6 weeks gestation, among them, the accuracy of 4CV+3VVT ultrasound section is the highest.

ObjectiveTo observe the effect of acupuncture plus language function training on the recovery of language function in patients with post-stroke aphasia.MethodNinety patients were divided into an acupuncture-rehabilitation group (intervened by acupuncture plus language function training) of 30 cases, an acupuncture group (intervened by acupuncture alone) of 30 cases, and a control group (intervened by language function training alone) of 30 cases. The Western Aphasia Battery (WAB) was adopted to evaluate the language function, and also taken as criteria for diagnosis and therapeutic efficacy. The aphasia quotient (AQ), and the 4 basic language functions including spontaneous speech, auditory comprehension, repetition and nomination were observed. ResultAfter intervention, AQ, and scores of spontaneous speech, auditory comprehension, repetition and nomination increased significantly in the three groups (P<0.05,P<0.01). there were significant differences in comparing the scores of spontaneous speech, repetition and nomination between the acupuncture-rehabilitation group and the control group (P<0.05). There were no significant differences in comparing the indexes between the acupuncture group and control group (P>0.05).ConclusionAcupuncture plus language function training can produce a positive effect on patients with post-stroke aphasia, showing therapeutic advantage in improvingspontaneous speech, repetition, and nomination.

Objective To investigate the systemic stress responses after laparoscopic resection in colorectal cancer patients.Methods Sixty patients were randomized into the laparoscopic resection group (30 cases) and open resection group (30 cases) from October 2001 to September 2002 in our hospital.Analgesic dosages, recovery time of intestinal peristalsis, postoperative temperatures, C reactive protein(CRP), IL 6 and white blood cell (WBC) counts were recorded after operation. Results The changes of postoperative temperatures and WBC counts showed no significant difference between two groups.But in the laparoscopic resection group, the recovery time of intestinal peristalsis, postoperative analgesic dosages, CRP and IL 6 significantly decreased ( P

Objective To evaluate the value of the use of fibrin glue in radical mastectorny for breast cancer. Method 52 patients with breast cancer were divided randomly into two groups. In one group (27 patients) fibrin glue was used during radical mastectomy, while in the other group (25 patients), fibrin glue was not used. The volume of drainage on the first postoperative day and total volume of drainage after the operation were collected and measured. The fluid accumulation underneath the flap was also observed. Result In patients with the use of fibrin glue, the amount of drainage was much less compared with those without the use of fibrin glue. Having marked diversity ( P

Objective To study effect of diclofenac sodium on content of prostaglandin E 2 KC/MC ratio and hyperpigmentation of the full-thickness skin autografts. Methods Content of PGE 2 was determined by radioimmune analysis method, and kinetic changes of KC/MC ratio were observed with histological and histochemical methods. Results In the operation control group, increase of KC/MC ratios was accompanied by of PGE 2 content at the early stage of the experiment; and the KMR became smaller than that in normal control group at the end of the experiment. While in the diclofenac soduim group, KMR was reduced due to the less damage of MC at the early stage, with decrease of PGE 2. Accordingly, KMR became larger at the late period of the experiment, and color of the autografts became lighter. Conclusion Application of diclofenac sodium on the grafts just after transplantation may reduce the damage of inflammation to the epidermal melanin units, control their proliferation and decrease the value of KMR, which lightens the color of the grafts.

ObjectiveTo observe the clinical efficacy of moxibustion plus acupoint autohemotherapy in treating allergic rhinitis due to lung-spleen qi deficiency.MethodTotally 120 eligible subjects were divided by using the random number table into a comprehensive group, a moxibustion group and a Western medication group. The comprehensive group was intervened by moxibustion plus acupoint autohemotherapy, the moxibustion group was by moxibustion,and the Western medication group was by Loratadine tablets. The acupoint autohemotherapy was give twice a week and the rest treatments were given once a day, 7 das a course, for 4 courses in total. A follow-up study was conducted 3 months later. The clinic efficacy was evaluated before and after intervention, as well as in the follow-up study.ResultThe three groups all achieved significant short-term efficacies after intervention, and the comprehensive group was equivalent to the moxibustion group, bothsuperior to the Western medication group(P<0.05). According to the follow-up study, the long-term efficacies of the comprehensive group and moxibustion group were both significantly higher than that of the Western medication group (P<0.01,P<0.05), and the moxibustion group was superior to the comprehensive group in comparing the long-term efficacy (P<0.01).ConclusionMoxibustion plus acupoint autohemotherapy and dry moxibustion both can produce significant short-term and long-term therapeutic efficacies in treating allergic rhinitis due to lung-spleen qi deficiency. The long-term efficacy of moxibustion is higher than that of moxibustion plus acupoint autohemotherapy in treating allergic rhinitis due to lung-spleen qi deficiency. Acupoint autohemotherapy requires strict aseptic operation, which restricts its application in family healthcare. Long-term use of moxibustion can activate yang qi, and thus plays a role in preventing diseases.

Objective To summarize the experience of assisted reproductive technology concurrent deep vein thrombosis, improve the level of nursing. Methods The clinical data of 5 cases of assisted reproductive technology concurrent deep vein thrombosis in Reproductive Medical Center of Peking University Third Hospital were retrospectively analyzed and the related literature was retrieved,the nursing experience and preventive measures were summarized. Results Through treatment and careful, all the patient were cured. Conclusions Strengthen the nursing care of patients with assisted reproductive technology concurrent deep vein thrombosis, actively prevent ovarian hyperstimulation syndrome occurred is the key to reduce thrombosis. Those patients with risks factors should be paid more attention, strengthen health education, reduce or alleviate complications.

Objective To assess risk factors in NSCLC with N2 metastasis.Methods A total of 177 NSCLC patients (109 males,68 females; age:(60.1 ± 10.6) years) who underwent both 18F-FDG PET/CT scan and surgery were enrolled.They were divided into two groups.One was with N2 metastasis (N2+) and the other was without (non-N2+).The gender,age,location,size,primary tumor SUVmax,histopathologic type and differentiation grade between N2+ group and non-N2+ group were compared with x2 test and two-sample t test.After assignment of significant factors and groups,correlations between each factor and group were analyzed with uni-and multivariate analyses,and then the risk factors were identified finally with logistic regression analysis.Results Significant difference among factors of tumor size ((36.1±19.7) mm vs (49.3±24.4) mm),SUVmax(8.81±6.23 vs 11.21±4.43) and differentiation grades (high:3 vs 0; moderate:74 vs 11; poor:65 vs 24) were observed between non-N2+ group and N2+ group (t =-2.969,-2.633,x2 =6.143,all P＜0.05).Besides,the univariate analysis showed significant correlations between tumor size,SUVmax,differentiation grade and N2 status (r=0.201,0.245,0.185,all P＜0.05).However,multivariate logistic regression revealed that only SUVmax had a predictive value (β=0.546,P＜0.01).The incidence of N2 metastasis went up along with the increase of SUVmax.There was no N2 metastasis in the patients with SUVmax ≤2.5,and the incidence rate of metastasis increased to 0.29 in those with SUVmax＞7.5.Conclusion SUVmax may be an independent risk factor to predict N2 metastasis in NSCLC patients.

Objective To study the protective effect of Shenkang injection on peritoneal mesothelial cells (PMCs) of continuous ambulatory peritoneal dialysis (CAPD) mice, and explore the possible mechanism. Methods Forty ICR mice were randomly divided into blank control (A) group, peritoneal dialysis (B) group, low dose of Shenkang (C) group ( 2.5%dialysate+5 mL/kg Shenkang injection), medium (D) group (2.5% dialysate+10 mL/kg Shenkang injection) and high (E) group (2.5% dialysate+ 20 mL/kg Shenkang injection). Mice were observed for 4 weeks. Fasting blood glucose, total cholesterol, triglyceride and C-reactive protein (CRP) were detected by biochemical assay. Tumor necrosis factor-alpha (TNF-α), transforming growth factor-beta 1 (TGF-β1), vascular endothelial growth factor (VEGF) and connective tissue growth factor (CTGF) levels of serum and dialysate were detected by ELISA. Pathological changes of peritoneal tissue were observed by HE staining. Expression and mRNA transcription levels of these four cytokines in the peritoneal tissue were detected by immunohistochemical staining and real-time PCR respectively. Results There were no significant differences in body weight, fasting blood glucose, total cholesterol and triglyceride between 5 groups of mice (P>0.05). Compared with group B, there was no significant difference in CRP level between group C and group E, but which was significantly decreased in group D (P<0.05). The serum and dialysate levels of TNF-α, TGF-β1, VEGF and CTGF were decreased in group C and group D. The serum and dialysate levels of TNF-αand TGF-β1 were significantly increased in group E (P<0.05), but there was no significant difference between VEGF and CTGF in group E. Compared with group E, except for CTGF in dialysate of group C, the serum and dialysate levels of TNF-α, TGF-β1, VEGF and CTGF were significant decreased in group C and group D (P<0.05). Damaged PMCs were found in group B, which were improved in various degrees in group C, group D and group E. Compared with group B, the protein expression and mRNA relative transcription levels of TNF-α, TGF-β1, VEGF and CTGF tended to decrease gradually in group C, group D and group E (P<0.05). Conclusion A certain concentration of Shenkang injection can protect PMCs by inhibiting the expression of TNF-α, TGF-β1, VEGF and CTGF in CAPD mice, so as to control the occurrence and development of peritoneal fibrosis.

Objective To study the spinal neurocyte apoptosis and the changes of p53 in chronic fluorosis rats,and the improvement after drinking no fluoride water.Methods One hundred twenty Wistar rats were divided into 4 groups by random number table method according to body mass,30 rats in one group fed with high concentration NaF water (200 mg/L) to make fluorosis model and classified as high fluoride group;other 30 rats were fed with distilled water as control group;another 30 rats were fed with high concentration NaF water (200 mg/L) for 12 weeks,then fed with distilled water for 12 weeks and classified as defluorination group;the rest 30 rats were classified as defluorination control group.The content of fluoride in urine was tested after the 4th,8th,and 12th weeks.Then the content of fluoride in urine of defluorination group and defluorination control group was tested.The high fluoride group rats and control group rats were killed after 12th week.Defluorination group rats and defluorination control group rats were killed after 24th week.Their spinal cord was collected.The expression of p53 protein in spinal cord was detected by immunohistochemistry and Westem blotting.Apoptosis of the neurocyte was quantitatively analyzed by flow cytometry (FCM).Results By FCM,apoptosis of neurocyte was increased in both high fluorosis group rats and defluorination group rats compared with those in control group rats [(3.36 ± 0.71)％ vs.(0.78 ± 0.65)％;(3.47 ± 0.56)％ vs.(0.83 ± 0.64)％,t =14.680,17.003,all P ＜ 0.01)],but no difference was found between these two groups [(3.47 ± 0.56)％ vs.(3.36 ± 0.71)％,P ＞ 0.05)].Immunohistochemistry and Western blotting revealed that p53 expression in spinal cord of high fluorosis group rats was increased compared with those in control group rats (422.69 ± 12.35 vs.177.82 ± 14.16;253.37 ± 10.42 vs.87.14 ± 7.39,t =77.212,72.988,all P ＜ 0.01).And p53 expression in spinal cord of defluorination group rats was increased compared with those in control group rats (418.75 ± 11.84 vs.163.47 ± 8.57;248.29 ± 10.23 vs.98.74 ± 11.52,t =95.663,53.167,all P＜ 0.01).But the differences were not statistically significant (418.75 ± 11.84 vs.422.69 ± 12.35;248.29 ± 10.23 vs.253.37 ± 10.42,t =1.261,1.906,all P ＞ 0.05).Conclusions There is apoptosis of neurocytes in the spinal cord of chronic fluorosis rats;overexpression of p53 probably plays an important role in the mechanism of damage induced by excessive fluorine.Apoptosis can not be recovered after defluorination for a short time,and persistent overexpression of p53 may be one of the reasons that apoptosis of neurocytes in the spinal cord can not decrease.