Objective To discuss postoperative analgesia effect of patient automatic controlled epidural analgesia (PCEA) and cocktail therapy (CT) after total hip replacement. Methods Eighty-eighty patients of selective total hip replacement were selected, and the patients were divided into PCEA group and CT group by random digits table method with 44 cases each. The postoperative resting state and active state visual analogue score (VAS), dosage of opioid, discharge time, postoperative complications, postoperative sleep quality score and hip joint mobility were compared between 2 groups. Results The resting state and active state VAS in PCEA group 12, 24 and 48 h after operation were significantly lower than those in CT group, resting state: (3.68 ± 1.45) scores vs. (4.23 ± 1.14) scores, (2.61 ± 1.04) scores vs. (3.92 ± 1.23) scores and (2.31 ± 0.97) scores vs. (3.56 ± 1.21) scores, active state:(4.54 ± 1.63) scores vs. (5.87 ± 2.11) scores, (3.51 ± 0.94) scores vs. (4.34 ± 1.07) scores and (3.01 ± 0.95) scores vs. (4.05 ± 1.17) scores, and there were statistical differences (P<0.05). But there were no statistical differences in VAS 72 h after operation and at discharge between 2 groups (P>0.05). The dosage of opioid in PCEA group was significantly lower than that in CT group:(9.58 ± 5.35) mg vs. (11.27 ± 4.48) mg, and there was statistical difference (P<0.05). The incidences of nausea vomiting and headache dizziness in CT group were significantly lower than those in PCEA group: 20.45% (9/44) vs. 45.45% (20/44) and 4.55% (2/44) vs. 13.64% (6/44), and there were statistical differences (P<0.05). There were no statistical differences in length of hospital stay, postoperative sleep quality score and postoperative hip joint mobility between 2 groups (P>0.05). Conclusions Choice of analgesic regimen of choice should not be made only according to the length of hospital stay. Individualized treatment is recommended. According to specific circumstances and needs of patients, patients with less anesthetics and a tendency to nausea should use PCEA, and for chronic pain patients CT may be more effective.

Objective:To investigate the protective effects of Ruanmai Capsules on cerebral ischemia-reperfuse injury in rats.Methods:A cerebral ischemia-reperfuse model in rats was established by ligaturing bilateral arterial carotis communis and vagus nerve. NO, 6-keto-PGF 1a and TXB 2 in serum were examined. Results: Ruanmai Capsule (0.81g/kg and 2.43g/kg,po) increased the levels of NO, 6-keto-PGF 1a. It also had the tendency to decrease TXB 2 in serum. Conclusions: Ruanmai capsules can alleviate brain injury caused by cerebral ischemia reperfusion. The effect is achieved perhaps by inhibiting platelet aggregation and increasing NO.

Gas Chromatography-Mass Spectrometry ; methods ; Humans ; Trichloroacetic Acid ; urine

objective: To study the effects of induction chemotherapy via subcutaneous implantable drug pump on local immune status. Methods: 47 cases with node negative oral cancer were treated with MTX, CDDP, PYM via subcutaneous implantable drug pump(in 27 cases), or via vein for case control trial(in 20 cases). Local immune status of tumor tissue was detected by immunohistochemical technique before and after treatment. Results: The effectiveness of treatment with the drugs via subcutaneous implantable drug pump (DSIP) was observed in 21 out of 27 cases (77.8%) and that with the drugs via vein(DV) was in 13 out of 20(65.0%) respectively( P 0.05), while the number of positive cells of CD 4 and CD 4/CD 8 in the cases treated with DV was decreased( P

Objective To explore the feasibility of Lugol's iodine staining in association with P53 oncoprotein test to detect early esophageal cancer and precancerous lesions through endoscopy. Methods Lugol's iodine was sprayed on esophageal mucosa under endoscopy in 78 patients with suspected early malignancy. Severe dysplasia which taken from unstained position by iodine were prepared immunohistochemically for P53 oncoprotein study. Then endoscopic mucosal resection (EMR) was performed to these severe dysplasia with P53 oncoprotein－ positive mucosa. Results There were 31 unstained areas (25 patients) including squamous cell carcinomas (n=3) and severe dysplasias (n=16). Expression of P53 was found in 7 severe dysplasia (43.8％ ),and 1 carcinoma in situ was found by EMR. Conclusion This modified method seemed feasible not only to detect early esophageal cancer,but also to resect severe dysplasia mucosa selectively by EMR.

Objective To assess volume state precisely and rapidly by ultrasonography of internal jugular vein (IJV) in healthy blood donor.Methods The values of the sonographic IJV collapse index and corrected IJV longitudinal length (cIJVLL) of 46 healthy blood donors were compared before and after blood donation.The correlations between IJV collapse index and cIJV LL were analyzed.Results The value of cIJV LLs before and after blood donation were significantly difference (6.56 ± 0.32 vs.6.11 ± 0.41,P ＜ 0.01).IJV collapse index before blood donation was not differently significant after blood donation (33.12 ± 2.21 vs.39.01 ± 3.83,P＞ 0.05).There was correlation between the value of cIJV LLs before and after blood donation (r =0.81).The value of IJV collapse index before and after blood donation,as well as cIJVLL was not well correlated (r =0.24,r =0.13,respectively).Conclusion The IJV collapse index is not a useful parameter for evaluation of hypovolemia,cIJV LL is more valuable marker for the detection of blood loss in emergency.

Objective To evaluate the long term results of coral bone used in anterior cervical interbody fusion. Methods The 180 patients (126 males and 54 females) who had undergone anterior interbody fusion in our hospital were followed up for 6 years. Their long-term imageological data, including changes in interbody height, interbody angle and range of movement, were analyzed and compared. Results In all the cases a solid fusion was achieved between coral reef and vertebra. The fusion was evaluated as successful in all the 180 patients. Conclusion The coral bone spacer provides the same solid fusion as autografting with iliac crest does.

Objective To study the clinical value of preserving intercostobrachial nerve(ICBN) during axillary lymph node dissection.Methods The clinical data of 146 cases of operated breast cancer of stage Ⅰ、Ⅱ、Ⅲa were analyzed.All cases were divided into twe groups randomly:preserved group(n=67) preserved ICBN in axillary lymph node dissection operation;control group(n=79) resected ICBN regulary in the operations.The number of lymph node dissection and the incidence of abnormal sense on the skin of inside upper arm were compared between groups.Results In one month,three months and six months followed up:the incidence of ahnormal sense on the skin of inside upper arm was 17.9%,11.9%,7.4% in preserved group,which was lower than that (74.9%,60.7%,59.5%) in control group significantly (P＜0.01).The diflrence between the two groups was significant.No local recurrence was occurred after opreations from eight months to five years examined.It was none difference that period of operation and the number of lymph node dissection between the groups.Conclusions Preserving ICBN in axillary lymph node dissection for breast cancer of stage Ⅰ、Ⅱ、Ⅲa could decrease the incidence of abnormal sence on the skin of inside upper arm.It eouldnt increase the incidence of local recurrence.It can improve the life quality of the patients after operations.

Objective To assess the diagnostic value of diffusion-weighted echo-planar MR Imaging (DWI) in ring-enhancing intracerebral lesions. Methods Magnetic resonance diffusion-weighted images of ninty-three patients presenting with ring-ehancing intracerebral lesions diagnosed by clinical or histopathologic findings were studied retrospectively, including 21 gliomas, 26 metastases, 13 pyogenic abscesses, 18 neurocysticercoses and 15 subacute intracerebral hematomas. The signal intensity ratio on diffusion-weighted images and exponential diffusion coefficient images was calculated respectively in ring walls, central contents, and perilesional edemas of ring-enhancing lesions, and normal contralateral cerebral parenchyma was used for comparison. ADC values of interest of lesions, contralateral cerebral parenchyma and CFS were calculated as well. Results In pyogenic abscesses and subacute intracerebral hematomas, the central content was always extremely hyperintense on diffusion-weighted images, and showed low ADCs[(0.56?0.20)?10~ -3 mm~2/s, (0.69?0.16)?10~ -3 mm~2/s, respectively]. On the other hand the central content of gliomas, metastases and neurocysticercoses was hypointense, and showed high ADCs [ (2.76?0.41)?10~ -3 mm~2/s, (2.31?0.39)?10~ -3 mm~2/s, (2.10?0.32)?10~ -3 mm~2/s, respectively]. The ADCs of the first two lesions were significantly lower than of the last three lesions (P

Objective:This study aims to analyze the differences in the alternative splicing pattern of ADAR2 among glioma cell lines U87, U251, A172, and normal human astrocyte HA1800. Methods:A-to-I editing level at the Q/R-Site of GluR-2 was analyzed by RT-PCR and sequencing. Real-time PCR was performed to detect the expression level of each alternatively splicing variant using a specific primer that was confirmed to amplify only the targeted template and not other alternatively spliced variant fragments. Results:We verified that the Q/R-Site of GluR-2 is under-edited in glioma cell lines. Real-time PCR revealed that the ADAR2 pre-mRNA splic-ing pattern has no significant difference at exons 1a and 2 between glioma cell lines and normal human astrocyte. We also detected that the amount of alternative splicing variants, including exon 5a, was higher than that of alternative splicing variants not including exon 5a in human glioma cell lines. However, the expression of alternative splicing variants, including exon 5a, was lower than that of alterna-tive splicing variants not including exon 5a in human astrocyte. Conclusion:Evident differences in splicing were observed at the site of exon 5a between glioma cell lines and normal human astrocytes. The difference in the alternatively splicing pattern at exon 5a may be attributed to the decreased activity of ADAR2.