Objective To explore the effect of beraprost sodium (BPS) on hypoxia-induced pulmonary artery hypertension (HPH) in rats and the expression of oxygen-sensitive Kv channels in pulmonary artery smooth muscle (PASM).Methods The HPH model of rats was established by exposing rats to low-pressure and low-oxygen cabin which was auto-modulated for 8h every day.Rats in the BPS group were given an intragastric administration of BPS [300 μg/(kg · d)],while those in the control group and HPH group were given an intragastric administration of 3 ml/kg of 0.9％ saline.After 4 weeks,the mean pulmonary artery pressure (mPAP) was measured and right heart ventricle hypertrophy index (RVHI) was calculated;pulmonary artery remodeling was evaluated by HE staining;the expressions of Kv 1.2,Kv 1.5 and Kv2.1 in the pulmonary artery were detected by Real-time PCR and Western blot.Results The HPH model was successfully established in rats exposed to chronic hypoxia for 4 weeks.Compared with those in HPH group,mPAP,RVHI and pulmonary artery remodeling were decreased in BPS group [mPAP:(13.48±2.18)mmHg vs.(23.87±2.23)mmHg vs.(17.09±1.20)mmHg;RVHI:0.28±0.02 vs.0.46±0.03 vs.0.36±0.04;％ area of medial smooth muscle:35.72±6.58 vs.68.52±5.64 vs.46.58±8.43;P＜0.05],and the mRNA and protein expressions of Kv 1.2,Kv 1.5 and Kv 2.1 were increased (relative protein expression level:Kv1.2,0.78±0.10 vs.0.15±0.03 vs.0.57±0.13;Kv1.5,0.61±0.10 vs.0.31±0.05 vs.0.59±0.13;Kv2.1,0.29±0.05 vs.0.10±0.02 vs.0.28±0.07;P＜0.05).Conclusion BPS can improve pulmonary arterial hypertension induced by hypoxia,and upregulate the decreased mRNA and protein expressions of Kv 1.2,Kv 1.5 and Kv 2.1 in pulmonary artery.

Objective To reverse P-glycoprotein-mediated muhiple drug resistance using RNA interference(RNAi)in cultured rat astrocytes.Methods Astroeytes overexpressing P-glyeoprotein induced by coriaria lactone were transfected with the short-hairpin RNA expression vector-p-SIREN shuttle designed to target Mdrl mRNA.The mRNA level of Mdrl gene was evaluated by real-time PCR;the P-glycoprotein was examined by immunocytochemistry and image analysis,meanwhile the rhodamine efflux was assessed by flow cytometry.Results The astrocyte model overexpressing P-glycoprotein were established and successfully transfected with the short-hairpin RNA expression vector-p-SIREN shuttle.The mRNA level of Mdrl gene was knocked down by 67.70%(P

OBJECTIVETo verify the mechanism of the hepatitis B viral clearance using clinical data.

METHODSViral level and HBV marker in serum were analyzed in 12 patients with acute hepatitis B.

RESULTSThe clearance of hepatitis B virus occurred before the patients were hospitalized in 66.7% of patients. The viral level and the A value of HBsAg;HBeAg declined gradually during hospitalization.

CONCLUSIONSIn most of patients with acute hepatitis B in the study, the virus was cleared without destruction of infected cells.

Adult ; DNA, Viral ; blood ; Female ; Hepatitis B ; virology ; Hepatitis B Antibodies ; blood ; Hepatitis B Core Antigens ; blood ; Hepatitis B Surface Antigens ; blood ; Hepatitis B e Antigens ; blood ; Hepatitis B virus ; genetics ; immunology ; isolation & purification ; Humans ; Male ; Middle Aged ; Prognosis ; Retrospective Studies

Alanine Transaminase ; metabolism ; Animals ; Liver ; drug effects ; Male ; Mice ; Plant Extracts ; pharmacology

The effects of the feed attractants on Whitmania pigra were studied. The average weight of Wh. pigra were 5.0 g. Arginine was selected as feed attractants, xanthan gum was selected as feed substrate. The times of Wh. pigra going into the inducing room were recorded. The water temperature was 22-25 degrees C during the whole experiment. Arginine that had better inducing effect was chosen to carry on in the gradient experiment. The results showed that the best inducing effect was found when the added amount of arginine was 0.3%, which was close to the arginine content of the natural body fluid of Wh. Pigra and Bellamya purificata, 2.97 mg x g(-1).
Animal Feed ; analysis ; Animals ; Arginine ; analysis ; metabolism ; Body Weight ; Feeding Behavior ; Leeches ; growth & development ; physiology

Objective To develop diabetic nephropathy hemodialysis(DN HD) self-management scale and evaluate its reliability and validity,and to provide a measurement tool for the assessment of self management status in patients with DN HD.Methods The original item pool was formulated through literature review and the theory of chronic disease self-management.After specialist evaluation and pre-investigation,the preliminary scale was developed,then it was used to investigate 150 DN HD patients,and a series of statistic analysis was conducted to deduce the items to 30,the reliability and validity of the scale also were tested.Results The response rate of acceptance test was 90.7％ and effective rate was 94.1％.The total Cronbach's α coefficient was 0.836,and the Cronbach's α coefficient of each dimension of the scale ranged from 0.701 to 0.822 ; the retest reliability was 0.970 ; the content validity index (CVI) was 0.925.Self-efficacy for managing chronic disease scale was used as the criterion,positive correlation was found between self-efficacy and self-management (P ＜0.01).Six common factors were extracted by factor analysis,and the cumtlative contribution rate was 54.042％,these were consistency with the expected structure.Conclusions The DN HD self-management scale has good reliability,validity and feasibility,which can be applied to evaluate the self-management status of DN HD patients.

Animals ; Cell Division ; drug effects ; Cells, Cultured ; Drug Delivery Systems ; Glycyrrhetinic Acid ; administration & dosage ; pharmacology ; Humans ; Liver ; cytology ; metabolism ; Liver Cirrhosis ; drug therapy ; Pilot Projects ; Rats ; Receptors, Cell Surface ; metabolism

Objective To compare short-term clinical outcomes and patient satisfaction between robotic-assisted total knee arthroplasty(TKA)and conventional TKA.Methods A retrospective analysis was conducted on data of 93 patients who underwent primary unilateral TKA for knee osteoarthritis from June 2022 to April 2023.Among them,46 patients underwent Mako robot-assisted TKA(robot group)and 47 patients underwent traditional manual TKA(traditional group).There was no statistical difference in general data between the two groups(P＞0.05).Follow-up data were collected to compare the differences in surgical indicators,postoperative pain,range of motion(ROM),Hospital for Special Surgery(HSS)score,and Forgotten Joint Score(FJS)between the two groups.Results The operation time of the robotic group was longer than that of the traditional group[(105.8±26.7)min vs.(77.0±14.9)min,P＜0.001].There were no significant differences in total blood loss,hip-knee-ankle(HKA)angle deviation value and deviation rate(HKA angle＞3°)between the two groups at 3 days after surgery(P＞0.05).The robotic group had lower Visual Analogue Scale(VAS)scores for pain than the traditional group at 3 days,2 weeks,and 6 months after surgery(P＜0.001,P＜0.001,P=0.021),and had better knee joint ROM at 3 days,2 weeks,and 3 months after surgery(P＜0.001,P=0.011,P＜0.001).The HSS score(P=0.001)and FJS score(P＜0.001)of the robotic group were better than those of the traditional group at 6 months after surgery.Conclusion Compared with conventional TKA,robot assisted TKA has milder early pain,better mobility,and higher overall postoperative satisfaction.

OBJECTIVETo analyze retrospectively the roles of different factors, especially the factors in the anastomotic cicatricial, stenosis after esophagectomy.

METHODSThe clinical data of 1111 patients, undergone esophagectomy with immediate reconstruction for cancer in the Sun Yat-sen Cancer Center from December 1997 to December 2003, were analyzed retrospectively. Up to 19 factors were evaluated by univariate and multivariate Logistic regression analysis with SPSS 11.5 for windows. Spearman rank correlation was used to evaluate the factors that were statistically significant in Logistic regression analysis.

RESULTSThe overall incidence of anastomotic cicatricial stenosis was 6.84%. By using univariate Logistic regression, sustained-hypoxemia (P=0.003), chest complications (P=0.000), anastomotic leakage (P=0.000), a history of diabetes (P=0.019), a history of chronic obstructive pulmonary disease (P=0.046) and cardiovascular complications (P=0.015) came out to be significantly related to anastomotic stenosis. Multivariate Logistic regression showed that sustained-hypoxemia (P=0.044), chest complications (P=0.009), anastomotic leakage (P=0.001) and a history of diabetes (P=0.036) were statistically significant. Spearman rank correlation estimation revealed that sustained-hypoxemia was positively correlated with chest complications (r=0.105) and anastomotic leakage,(r=0.106), and chest complications were positively correlated with anastomotic leakage (r=0.228).

CONCLUSIONSustained-hypoxemia is one of the significant factors for anastomotic cicatricial stenosis after esophagectomy.

Aged ; Anastomosis, Surgical ; Esophageal Stenosis ; etiology ; Esophagectomy ; adverse effects ; Esophagus ; surgery ; Female ; Humans ; Hypoxia ; Logistic Models ; Male ; Middle Aged ; Postoperative Complications ; Prognosis ; Retrospective Studies ; Risk Factors

OBJECTIVETo study the role of a pathologic niche inducing mouse embryonic stem cells (ESC) to express hepatic cell functions in vitro.

METHODSEmbryoid bodies were developed from 5 to 7 day hanging-drop culture of mouse ESC, and their dissociated cells were planted in three differential systems: nothing added; with 20 ng/ml hepatocyte growth factor (HGF); and 5% rat cholestatic serum plus 20 ng/ml HGF added. Their differentiation was observed with inverted microscopes daily, and their hepatic functions were analyzed against their synthesis of glycogen, triglycerides, albumin, and urea nitrogen, and by their staining of indocyanine green (ICG) and fluorescein diacetate (FDA).

RESULTSESC spontaneous differentiation was hardly being controlled to form three germ layers. HGF prompted the ESC to develop further into visceral endoderm and mesoderm (myocardium), but both of them only expressed a low level of hepatocyte-specific metabolic functions. With cholestatic serum added into the HGF-induced system, differentiated cells grew into similar angular cells, and had a higher level synthesis of glycogen, triglycerides, albumin and urea nitrogen with positive ICG and FDA staining.

CONCLUSIONSSpontaneous or HGF-induced ESC differentiation has only limited hepatic functions expressed. A pathologic niche in vitro induces ESC to develop into hepatic lineages, with a higher level of hepatic metabolic functions.

Animals ; Cell Differentiation ; physiology ; Cells, Cultured ; Cholestasis ; blood ; Culture Media ; pharmacology ; Embryo, Mammalian ; Hepatocytes ; cytology ; Mice ; Serum ; Stem Cells ; cytology