Environmental Pollutants ; Flame Retardants ; Hydrocarbons, Brominated

This article reports, for the first time in China, the study on the change of glucocorticoid receptor (GCR) in peripheral leukocytes from 23 patients with chronic nephritis. The number of GCR was measured by the single point analysis using ~3H-Dexamethasone (~3H-Dex) as the ligand. It was found that the number of ~3H-Dex specific binding was 8825 ? 2429 sites/cell, much higher than that in normal subjects (5154 ? 1635 sites/cell, n = 22) P0.05). The concentration of plasma cortisol was determined simultaneously. It was within the normal range. There seems to be no relationship in these patients between the number of GCR and the concentration of plasma cortisol statistically (r = 0.074, n = 25). The results showed that the level of GCR in leukocytes during chronic nephritis was changed. This change had no relationship with the concentration of plasma cortison. The possible mechanism and clinic significance of the results have been discussed.

We proposed a multi-resolution-wavelet-transform based method to extract brainstem auditory evoked potential (BAEP) from the background noise and then to identify its characteristics correctly. Firstly we discussed the mother wavelet and wavelet transform algorithm and proved that bi-orthogonal wavelet bior5. 5 and stationary discrete wavelet transform (SWT) were more suitable for BAEP signals. The correlation analysis of D6 scale wavelet coefficients between single trails and the ensemble average of all trails showed that the trails with good correlation (> 0. 4) had higher signal-to-noise ratio, so that we could get a clear BAEP from a few trails by an average and wavelet filter method. Finally, we used this method to select desirable trails, extracted BAEP from every 10 trails and calculated the I-V inter-waves' latency. The results showed that this strategy of trail selection was efficient. This method can not only achieve better de-noising effect, but also greatly reduce the stimulation time needed as well.
Algorithms ; Brain Mapping ; Evoked Potentials, Auditory, Brain Stem ; Humans ; Wavelet Analysis

Sepsis is systemic inflammatory response syndrome associated with infection.Early recognition of severe sepsis or septic shock,early hemodynamic resuscitation and the immediate initiation of effective intravenous anti-infective therapy are the cornerstones in the management of patients with sepsis.The infectious microorganisms associated with sepsis in pediatric patients vary with the patient's age and immune status.Initial empirical antimicrobial therapy should include one or more drugs that have activity against all likely pathogens and that penetrate in adequate concentrations into the presumed source of sepsis.Empirically therapy should be de-escalated to the most appropriate single therapy as soon as the susceptibility agent is known.

The professional skill schools started an activity,which is the important measure to popularize the strategy of“the science and education making our country prosper”,that is,the definition of professional skill and implement of institution of professional certificates.It is the development direction of professional education that implementing of the institution of double-certificates',creating the new mode of cultivating the individuals,and strengthening the tuition of students'skill to make the students to acquire the education diplomas and the pro- fessional certificates.This issue points out the way and some opinions in this work,in the terns that imploring practice of the identification of the professional skill of microorganism inspecting worker' and the joint of microorganism education reform.

Organophosphate pesticides(OPs)are used extensively in agriculture,it became the most heavily used insecticides in China after the use of organochlorine pesticide was restricted in 1980s in China.Recent years,with an increasing usage of organophosphate pesticides in China,much concern has been showed for the level of OPs in human body and the adverse effects of OPs on human health especially on fetuses and children have raised a great.The level of OPs in children and the adverse effects of OPs exposure on neurobehavioral development of children were reviewed in the present article.

Neonatal respiratory distress syndrome (RDS) is most common in premature infants,the smaller the gestational age,the higher the incidence.Continuous positive airway pressure (CPAP) started in the delivery room has been shown in multicentre randomized controlled trials to reduce the need for mechanical ventilation (MV) and surfactant.The European Consensus Guidelines on the Management of Neonatal Respiratory Distress Syndrome in Preterm Infants-2013 Update recommended that CPAP should be started from birth in all babies at risk of RDS and a starting pressure of at least 6 cmH2O(1 cmH2O =0.098 kPa)should be applied.CPAP with early rescue surfactant should be considered the optimal management for babies with RDS.Using CPAP immediately after birth with subsequent selective surfactant administration may be considered as an alternative to routine intubation with prophylactic or early surfactant administration in preterm infants.If endotracheal intubation is needed,early administration of surfactant is preferable.Nasal intermittent positive pressure ventilation may reduce the risk of extubation failure in babies failing on CPAP.Several strategies have been employed specifically to improve the success of noninvasive ventilation and shorten the duration of MV.Caffeine should be used to facilitate weaning from MV and to reduce bronchopulmonary dysplasia.A short tapering course of low-or very low-dose dexamethasone should be considered to facilitate extubation in babies who remain on MV after 1 ～ 2 weeks.Very early steroid treatment and treatment with high doses cannot be recommended.

Objective To establish an immortalized rat astrocyte strain(IAST) expressing enkephalin regulated by doxycycline(Dox) and observe its analysesic effect on rat chronic neuropathic pain. Methods Retrovirus infection method was employed to develop an immortalized rat astrocyte strain expression enkephalin regulated by doxycycline.hPPE gene expression level of IAST/Tet-On/hPPE strain was detected by Real time-PCR and radioimmunoassay.Its analgesic potential was investigated by mechanical paw withdrawal thresholds after these cells were implanted into the subarachnoid space of chronic constrictive injury(CCI) rats.The expression of Fos protein in the dorsal horn of spinal cord was determined by immunohistochemistry. Results An immortalized rat astrocyte strain secreting enkephalin under the control of doxycycline was established successfully.After transplantation of IAST/Tet-On/hPPE cell into the subarachnoid space of chronic constrictive injury(CCI) rats, the sensitivity of mechanical allodynia and the expression of Fos protein were significantly decreased(P

Objective To construct a recombinant retroviral vector with human preproenkephalin gene regulated by tetracycline. Methods Human preproenkephalin gene was amplificated by polymerase chain reaction (PCR) and was cloned into retrovirus tetracycline responsive plasmid. Then this recombinant plasmid and regulatory plasmid pRev Tet-On were transferred into packaging cell PT67 respectively. The transfected PT67 / Tet-On and PT 67 / TREhPPE cells were selected by the corresponding antibiotics and identified by RT-PCR. The selected cells were enriched and virus liter was assayed using NIH3T3. Results The restriction endonuclease digestion, PCR analysis and DNA sequencing confirmed that the recombinant RevTRE/hPPE vector was constructed successfully. The virus liter of PT 67/TREhPPE was 3.2 ? 103 CFU?ml-1 and the virus titer of PT67/Tet-On was 2.6?105 CFU?ml-1.Conclusion A recombinant retroviral vector with human preproenkephalin gene regulated by trtracycline and stable virus producing lines have been successfully constructed, providing a good basis for further research on regulated cell therapy of chronic pain.

Objective To establish and identify tetracycline controlled gene inducible system in immortalized rat astrocyte strains. Methods The PT67 cells were transfected with pRevTet-On vector. The transfected cells were selected in medium containing G418. RT-PCR assay was used to confirm that Rev Tet-On virus was packed correctly. The viral titer was assayed by infecting NIH3T3 cells. The immortalized astrocyte was infected by RevTet-On virus and individual clones were selected. All individual clones were transiently transfected with pRev TRE-Luc, which carried luciferase gene. The clone with low background and high induction expression was selected and its inducibility was tested. Results The RT-PCR assay showed that RevTet-On virus was packed successfully. The highest viral titer of RevTet-On was 7.4?05 CFU/ml. Rev Tet-On infected immortalized astrocyte and 48 individual clones were isolated. Clone 6 was selected for its highest induction of the luciferase activity in response to doxycycline and the lowest leakiness (activity in the absence of doxycycline) and its inducible fold was 20.6. The expression of luciferase was induced in a dose-dependent manner by doxycycline at the concentrations between 100 and 2 000 ng@ml-1. The expression of luciferase began 1h after doxycycline administration (1000 ng?ml-1) and reached the maximum level 48 h later. Conclusion The tetracycline controlled inducible system is established successfully in immortalized rat astrocyte strains, which is useful for the study of control exogenous gene expression.