AIM: Based on the principle of ergonomics theory, to propose the hypothesis of rehabilitation medicine to promote standardization of rehabilitation medicine. METHODS: Through survey, observation, and experiment, human physiological and psychological factors were analyzed. Scientific ergonomics design, planning, and management were used to enhance the clinical value of rehabilitation medicine efficacy in evidence-based medicine. RESULTS: Ergonomics-based rehabilitation can resolve common defects in rehabilitation clinical work and management, and promote the standardization of medical rehabilitation objectively and scientifically. CONCLUSION: Ergonomics-based rehabilitation medicine is more objective, scientific and practical, and the repeatability of rehabilitation treatment is improved. It is worth further clinical study.

Objective To investigate the altered lipid in systemic sclerosis(SSc),and the relationship between the altered lipid and inflammation.Methods A total of 58 SSc patients were recruited into SSc group,and 58 healthy persons were recruited into control group from 2012 to 2014.Triglyceride(TG),total cholesterol(TC),low density lipoprotein-cholesterol(LDL-c)and high density lipoprotein-cholesterol(HDL-c),lipoprotein a[LP(a)],C-reactive protein(CRP)and erythrocyte sedimentation rate(ESR) were assessed using standard techniques in all cases,antinuclear antibody(ANA)and anti-Scl-70 antibody were assessed only in SSc patients.The levels of lipids between SSc patients and healthy controls,patients with antibody and patients without antibody were compared by using t test,corrections between lipid and inflammation were analyzed by Spearman′s correlation test.Results The clinical manifestations of SSc were mostly Raynaud′s phenomenon(91.4%),large area of black hard shin(87.9%),gastrointestinal manifestations(75.9%).The rates of an increased level of TG and decreased level of HDL-c were 29.3%,89.7% respectively.The level of TG in SSc was significant higher than in healthy controls(P =0.021),and the level of HDL-c in SSc was significant lower than in healthy controls(P =0.033).The levels of lipids in patients with ANA and anti-Scl-70 antibody had no significant difference with the patients without ANA and anti-Scl-70 antibody (P >0.05 ).Spearman′s correlation test demonstrated that HDL-c level correlated negatively with serum CRP (r=-0.285,P =0.039)and ESR (r =-0.271,P =0.043)in SSc.Conclusion Dyslipi-demia is a common feature in SSc patients that are characterized by an increase in TG and a decrease in HDL-c,inflammation might partly account for the changes.

Enterovirus 71 (EV71) is the main causative agent of hand, foot, and mouth disease (HFMD). This article presented the inhibitory activity of pentapeptides on the EV71 infection in rhabdomyosarcoma (RD) and suckling mice. The EV71 VP1 capsid protein expression levels and mRNA levels were analyzed by Western blotting and real-time PCR. The antiviral activity of pentapeptides in vivo was evaluated by weight changes and EV71 VP1 protein expression levels in intestines of suckling mice. Results revealed that the pentapeptide P010157 was able to inhibit EV71 replication in RD cells. After being incubated with the P010157 at a concentration of 100 microg x mL(-1) for 48 h, the level of EV71 vp1 mRNA in RD cells decreased by (92.0 +/- 6.3)%. The estimated EC50 was 2.2 microg x mL(-1). P010157 was able to inhibit EV 71-induced cytopathic effect (CPE) in RD cells. The cytotoxic activity of the compound was evaluated against RD cells by MTS assay. The results showed that P010157 had no obvious toxicity. In addition, the treated mice with P010157 did not exhibit weight loss, as was observed in untreated mice. EV71 replication reduced significantly as revealed by Western blotting. These findings suggest that P010157 could prevent EV71 proliferation in vitro and in vivo. P010157 is a novel compound for antiviral therapies against EV71, which merited further investigation.
Animals ; Antiviral Agents ; pharmacology ; Capsid Proteins ; genetics ; metabolism ; Enterovirus A, Human ; physiology ; Enterovirus Infections ; metabolism ; Humans ; Mice ; Oligopeptides ; pharmacology ; RNA, Messenger ; metabolism ; Rhabdomyosarcoma ; metabolism ; pathology ; virology ; Tumor Cells, Cultured ; Virus Replication ; drug effects

Aim To study whether or not the green tea catechins(EGCG)has physiological benefits and the underling protective mechanism.Methods The subunit protein levels of ?4、?7 of nAChR were detected by BCA protein assay,Dot Blot assay and MTT assay.Results The results showed that the green tea catechins can significantly reduce the subunit protein levels of nAChR and decrease the cell activity induced by A?1-40.Conclusions EGCG can provide neuroprotection in vitro by up-regulating nAChR sununit levels and inhibiting the neurotoxin of A?1-40.

Objective: To study review the experience of the preparation method of total nutrient admixture(TNA).Methods: 273 patients receive TNA in 2005.More than 4 200 bags of TNA were prepared.Results: There were no infusion reaction and complication in clinical application because of unsuitable preparation.100 bags of TNA were detected for bacteris and all were negative.Conclusion: The ingredients of parenteral nutrition are various and the preparation is important.Strict aseptic technique and preparation procession are wanted.

Electret filtration material is a kind of promising new air filtration material due to the advantages of high filtration efficiency,low flow resistance,power saving and anti-bacteria.In the paper the concept of electret,primary raw material of electret filtration material,the characteristics of electret filter material,charging method to make electret and the influence of charging technology on the charge storage characteristic of electret filter material were introduced,the application of electret filtration material on air purification was described also.

Under stimulation of PHA,PHA + TPA,or PHA + PTS the time ke-netics of DNA,RNA,protein synthesis and lymphokine secretion by human lymph node cell expressed different responses. The PTS did not increase the DNA and RNA synthesis, but increased the protein synthesis ( 60% ) . TPA increased the RNA synthesis ( 18% ) , therefor increased the protein synthesis ( 40% ) . Both of TPA and PTS increased the production of 5 kinds of lymphokines (IL-1 , IL-2 , IL-3, BCGF, TFNr). We infer that PTS and TPA increase the lymphokine production through different gene regulation. TPA Promotes the translation of lymphokine mRNA。

Promoting effect of panaxatriol ginsenoside ( PTGS ) on inter-leukine-3(IL-3 ) induction of phyto-hemagglutinin ( PHA ) stimulated human lymph node cells was observed. The results showed that PTGS enhanced the IL-3 production on each time of IL-3-induction kinetics, maximally by 30% ( 72h ) . Furthermore, it was observed that IL-3 mRNA from PHA+PTGS-stimulated lymph node cells translated more IL-3 than PHA-stimulated lymph node cells on each time of IL-3 mRNA-induction kinetics, maximally by 40% ( 60h ) , using the methods of wheat germ cell-free in vitro translation system and IL-3 bioassay.

Objective To investigate the comparability of test seven tumor marks results of two electrochemiluminescence immu-noassay analyzer between Roche Cobas E411 and Cobas E601 .Methods According to United States Clinical and Laboratory Stand-ards Institute (CLSI) EP9-A2 document ,E411 was acted as comparison method (X) and E601 was as the experimental method (Y ) .Alpha fetoprotein (AFP ) ,carcinoembryonic antigen (CEA ) ,carbohydrate antigen125 (CA125 ) ,carbohydrate antigen199 (CA199) ,carbohydrate antigen153(CA153) ,total prostate specific antigen(TPSA) and free prostate specific antigen(FPSA) levels of patient samples were tested and the results were analyzed .Results The correlation between the two systems was good and the bias was acceptable in the medical decision level .Conclusion Two systems have good concordance in detecting the seven tumor markers ,w hich can meet the clinical need .

Objective To observe effects of 17?-estradiol on phenylephrine-induced hypertrophy in cultured neonatal rat cardiomyocytes and to explore its mechanisms. Methods Using cultured neonatal rat cardiomyocytes treated with different factors as a model,surface area of cardiomyocytes was measured by computer photograph analysis software;the protein synthesis rate was assayed with leucine intake method;the proto-oncogene c-fos protein expression was assessed with immunocytochemistry technique;the markers of cardiomyocyte hypertrophy,embryonic genes such as ?-MHC\,?-skA and ANP mRNA expressions were assessed with semiquantitative reverse transcription-PCR. Results 17?-estradiol inhibited obviously phenylephrine-induced increase of surface area and the protein synthesis rate of cardiomyocytes,reduced c-fos protein expression of hypertrophic cardiomyocytes,and down-regulated ?-MHC、?-skA mRNA expressions and up-regulated ANP mRNA expression.Conclusion 17?-estradiol inhibits hypertrophy of cardiomyocytes and its mechanisms might be related with the suppression of c-fos protein expression,the reversion of the embryonic switching of contractile protein genes(?MHC and ?-skA),the increase of ANP mRNA expression.