Objective To evaluate the effect of PKC? antisense oligonucleotide (ASODN) administered intrathecally on the hyperalgesia induced by chronic constructive injury (CCI) and to investigate the underlying mechanism.Methods Twenty four female SD rats weighing 150-180 g were used. CCI was produced by 4 loose ligatures placed on the right sciatic nerve. A catheter was inserted into subarachnoid space at L3-4 for intrathecal drug or normal saline(NS) administration. Three days after intrathecal catheter implantation when the function of the animal's lower limbs recovered, NS or drug was injected through the catheter every day for 6 days. Then the animals were decapitated and the lumbar segment (L2-6 ) of the spinal cord was removed. The animals were randomly divided into 4 groups of 6 animals : Ⅰ CCI + NS (group C); Ⅱ CCI + PKC? sense oligonucleotide (SOON) 20 ?g (group S); Ⅲ CCI + ASODN 5 ?g (group A1) and Ⅳ CCI + ASODN 20 ?g(group A2). The mechanical withdrawal threshold was assessed by Von Frey hair stimulation. The expression of PKC? and PKCa protein in the spinal cord was determined using Western blot. Results The threshold to Von Frey hair stimulation was significantly reduced after sciatic nerve ligation. Intrathecal ASODN administration significantly reduced the hyperalgesia induced by CCI in group A1 and A2 in a dose-dependent manner as compared with group C. The expression of PKCy protein in lumbar spinal cord was significantly lower in group A1 and A2 than in group C. There was no significant difference in PKCa protein expression among the four groups. Conclusion The hyperalgesia induced by CCI can be decreased by intrathecal administration of PKCy antisense oligonucleotide. The reduction in expression of PKCy protein may be involved in the mechanism.

The retrieval of the existing literature on the types of fungi polysaccharides, chemical structure and anti-canceractivity, anti-cancer mechanism was outlined, providing scientific information for further study on anti-cancer action of polysaccharides of fungi.

In a PACS system, doctors can avoid the mistakes in filing chest radiographs by comparing the new image with the old ones from the same patient. An automatic method with digital image processing technology is introduced in the paper, which is realized by general location of anatomical structures, local image registration and BP artificial neural network, so as to achieve good results for identity recognition.
Neural Networks (Computer) ; Patient Identification Systems ; Radiographic Image Interpretation, Computer-Assisted ; methods ; Radiography, Thoracic ; methods

OBJECTIVE：To pro vide reference for reducing the error of Markov model in pharmacoeconomic evaluation. METHODS：Referring to foreign literatures ，the errors in Markov model were explained. The commonly used correction methods were introduced ：half-cycle correction ，trapezoidal rule ，Simpson’s 1/3 rule，Simpson’s 3/8 method and life table method and their implementation in Excel and TreeAge software. RESULTS & CONCLUSIONS ：The error of Markov model was caused by the discretization process and could be corrected by the within-cycle correction methods. Half-cycle correction ，was the most commonly used correction method and could be corrected by adding half of the results of the first cycle and subtracting half of the results of the last cycle. By trapezoidal rule ，the interval was represented by the mean value of the first end point value of the interval ，and the area of right trapezoid was the cummulative result. Simpson’s 1/3 rule and Simpson ’s 3/8 rule took another point in the interval on the basis of trapezoidal correction and the continuous curve where these three points were represented the whole curve. By life table method ，the person-years of survival was the product of the group distance of the age group and the mean value of the number of survivors of the age group and the next age group ，and the total person-years of survival was the sum of person-years of survival of each age group. In Excel ，the fuction was set according to the method principle to realize the correction ；in Tree Age software，the function expression of Init Rwd ，Inor Rwd and Final Rwd were set to realize the correction. When using Markov model for pharmacoeconomic evaluation ，if the selection of periodic correction method is based on the ease of modeling and the universality of application ，the trapezoid rule method is recommended ；based on the accuracy of results ，it is suggested to use Simpson’s 1/3 method，so as to improve the accuracy of Markov model.

OBJECTIVE：To p rovide reference for th e development and application of the pediatric quality of life inventory （PedsQL）. METHODS ：Using“PedsQL”as keyword ，retrieved from foreign databases as Medline ，ScienceDirect，Cochrane Library，ISI Web of Science ，SpringerLink and Embase ，Chinese databases as CNKI ，Wanfang database ，VIP and SinoMed ， research literatures about the application of PedsQL at home and abroad were collected during Jan. 1999-Dec. 2019. RESULTS & CONCLUSIONS：A total of 2 117 literatures were included ，including 1 836 foreign literatures and 281 domestic literatures （242 in Chinese，39 in English ）. The number of published literatures was increasing year by year. PedsQL had a wide range of applications and divided into 4 main areas according to purpose ，including analysis of health influencing factors （63 items），assessment of diseases burden （1 720 items），evaluation of intervention measures （285 items），theoretical study of other scales （49 items）. The application of PedsQL in clinical trials had made fast progress ，but there were still some problems in China ，such as improper selection and use of the scale ，less application in pediatric clinical practice ，and insufficient evaluation of intervention measures. It is suggested that future researcher should consider both universality and disease specificity ，self-administered and parental surrogate version of PedsQL when selecting tools ，and apply PedsQL to perform routine clinical health-related quality of life screening to optimize the utilization efficiency of pediatric health 一 resources，and construct PedsQL mapping function based on Chinese population preference to realize the economic evaluation of drug intervention measures.

Exons ; genetics ; Female ; Genetic Predisposition to Disease ; Humans ; Male ; Membrane Proteins ; genetics ; Middle Aged ; Mutation ; Pedigree ; Spastic Paraplegia, Hereditary ; etiology ; genetics

BACKGROUNDParoxysmal kinesigenic dyskinesia (PKD) is a rare movement disorder characterized by recurrent dystonic or choreoathetoid attacks triggered by sudden voluntary movements. Under the condition of psychological burden, some patients' attacks may get worsened with longer duration and higher frequency. This study aimed to assess nonmotor symptoms and quality of life of patients with PKD in a large population.

METHODSWe performed a cross-sectional survey in 165 primary PKD patients from August 2008 to October 2016 in Rui Jin Hospital, using Symptom Check List-90-Revised (SCL-90-R), World Health Organization Quality of Life-100 (WHOQoL-100), Self-Rating Depression Scale, and Self-Rating Anxiety Scale. We evaluated the differences of SCL-90-R and WHOQOL-100 scores in patients and Chinese normative data (taken from literature) by using the unpaired Student's t-test. We applied multivariate linear regression to analyze the relationships between motor manifestations, mental health, and quality of life among PKD patients.

RESULTSCompared with Chinese normative data taken from literature, patients with PKD exhibited significantly higher (worse) scores across all SCL-90-R subscales (somatization, obsessive-compulsive, interpersonal sensitivity, depression, anxiety, hostility, phobic anxiety, paranoid ideation, and psychoticism; P= 0.000 for all) and significantly lower (worse) scores of five domains in WHOQoL-100 (physical domain, psychological domain, independence domain, social relationship domain, and general quality of life; P= 0.000 for all). Nonremission of dyskinesia episodes (P = 0.011) and higher depression score (P = 0.000) were significantly associated with lower levels of quality of life. The rates of depression and anxiety in patients with PKD were 41.2% (68/165) and 26.7% (44/165), respectively.

CONCLUSIONSDepression, anxiety, and low levels of quality of life were prevalent in patients with PKD. Co-occurrence of depression and anxiety was common among these patients. Regular mental health interventions could set depression and anxiety as intervention targets. Considering that the motor episodes could be elicited by voluntary movements and sometimes also by emotional stress, and that symptoms may get worsened with longer duration and higher frequency when patients are stressed out, intervention or treatment of depression and anxiety might improve the motor symptoms and overall quality of life in PKD patients.