Objective To evaluate the recent impact and long-term effects of recombinant human acidic fibroblast growth factor (rh-aFGF) of cesarean section on women's abdominal incision healing.Methods In 516 cases of cesarean section in women,randomly divided into placebo group (172 cases),spray group (172 cases) and flush group (172 cases).The placebo group received 0.9％ sodium chloride washing abdominal incision suture.The spray group incision suture direcdy after the administration of rh-aFGF spray,per square meter of 3-4 sprays,3-5 times/d.The flush group was given before 2 rh-aFGF dissolved in 10-20 ml of 0.9％ sodium chloride directly after washing the incision suture,then given spray treatment.The recent follow-up of 15 d observed maternal abdominal incision healing.Long term follow-up of 3 months to observe the incision scar formation.Results The abdominal incision heating time was (8.6 ±3.5) d,that was obviously shorter than that in spray group (10.4 ±3.7) d and placebo group (12.7 ± 4.9) d (P ＜ 0.05).The delay healing accounted for 4.2％ (7/166) in flush group,significantly lower than that in placebo group (34/168,20.2％) and spray group (16/165,9.7％) (P＜ 0.05).There was no significant difference in the incidence of fat liquefaction,postoperative infection among three groups (P ＞ 0.05).Flush group exudation,abdominal wall adhesions was lower than placebo group and spray group [2.4％(4/166) vs.10.1％(17/168),5.5％(9/165),3.6％(6/166) vs.17.3％(29/168),9.7％(16/165)](P＜0.05).Long term follow-up,flush group scar area than spray group reduced by 13.5％,than placebo group reduced by 27.2％.Conclusions A recent rh-aFGF can reduce the cesarean section abdominal incision healing time and reduce abdominal wall adhesions and exudation;rh-aFGF the best dosage is intraoperative irrigation treatment spray combined with postoperative.

Objective To investigate the effect of Niaochangshu capsule on urodynamics in bladder filling period of patients with post-menopause urethral syndrome of renal deficiency and blood stasis type.Methods A total of 36 patients with post-menopause urethral syndrome of renal deficiency and blood stasis type were randomly assigned to two groups:the trial group(24 patients were treated with Niaochangshu capsule) and the control group(12 patients were treated with Nilestriol).All patients were treated for 8 weeks.The changes of the urodynamic indexes in bladder filling period(the capacity of the bladder,the compliance of the bladder and the sensitivity of the bladder) were compared between the two groups.Results After treatment,the urodynamic index,including the maximum cystic capacity,the volume at the first desire to void and the cystic compliance were evidently improved in both of the trial group and the control group(P0.05).Conclusion Niaochangshu capsule is effective in treating the patients with post-menopause urethral syndrome of renal deficiency and blood stasis type.It can increase the cystic capacity and compliance,step down the sensitivity of the bladder.Its effect is similar to Nilestriol.

An evaluation scale of medical services quality was developed on the basis of "patients' experience". The questionnaires were developed by, among others, searching relevant literature, collecting well-established assessment scales, measuring patients' experience and satisfaction, brain-storming, literature analysis. Delphi method was adopted for expert consultation. Scale items were screened and revised. The key indexes were converted. Field surveys were conducted for testing the reliability and validity of the scale. Our modified evaluation scale for measuring medical services quality based on "patients' experience" included 6 dimensions (tangibility, reliability, responsiveness, assurance, empathy and continuity), and 50 items. The novel scale based on "patients' experience" may better serve the purpose of assessing medical services quality.

Objective To evaluate laparoscopy assisted radio frequency ablation (LRFA) and percutaneous radiofrequency ablation ( PRFA ) for the treatment of hepatocellular carcinoma ( HCC ).Methods A total of 525 HCC patients were enrolled,including LRFA group of 78 cases with 106 tumor nodules,and PRFA group of 447 cases with 565 tumor nodules.Results ( 1 ) In LRFA group complete ablation rate was 97.17％ (103/106); in LRFA group complete ablation rate was 93.09％ (526/565)(x2 =2.523,P =0.112).(2)The 1,3,5 year overall survival rate was 96.15％,55.12％ and 38.46％in LRFA group and 93.73％,48.54％ and 31.54％ in PRFA group respectively ( x2 =0.699,1.151,1.447 ; P =0.403,0.283,0.229 ).The 1,3,5 year disease-free survival rate was 94.87％,43.58％ and 28.21％ in LRFA group and 93.73％,48.54％ and 31.54％ in PRFA group respectively (x2 =0.915,0.303,0.174; P=0.339,0.582,0.676).The average disease-free survival time was 22.25 months in LRFA group and 21.53 months in PRFA group respectively.(3)The serious complications was 0％ (0/78)in LRFA group and 1.34％ ( 6/447 ) in PRFA group respectively.( 4 ) Recurrence rate was 23.07％(18/78) in LRFA group,and 34.89％ (156/447) in PRFA group ( x2 =4.189,P =0.041 ).Conclusions The therapeutical effect of LRFA equals that of PRFA,while enjoying lower recurrence rate,no serious complications and higher treatment safety.

Carboxysomes are extremely efficient microcompartments committed to CO2 fixation due to tailored CO2-concentrating mechanism (CCM). In cyanobacteria and some chemoautotrophs, carboxysomes as organelle-like microbodies encapsulate ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and carbonic anhydrase (CA). Together with active inorganic carbon uptake transporters, carboxysomes accumulate HCO3(-) in the cytoplasm, leading to high efficiency of carbon fixation. Based on the elucidation of structures and functionalities, heterologous production of carboxysomes has been achieved so far. In fact, the genes encoding either vacant carboxysome shell or only interior components have been characterized. This review summarizes the discovery along with types, showcases molecular structures and roles of carboxysomes in CCM, and presents their broad applications in metabolic engineering.
Biological Transport ; Carbon ; metabolism ; Carbon Cycle ; Carbon Dioxide ; metabolism ; Cyanobacteria ; metabolism ; Metabolic Engineering

Objective To evaluate the clinical factors associated with pathological complete response (pCR) after preoperative neoadjuvant chemoradiotherapy for rectal cancer.Methods A retrospective analysis was performed on the clinical data of 116 patients with rectal cancer,who underwent neoadjuvant chemoradiotherapy followed by radical surgery from January 2009 to December 2012.All patients received pelvic intensity-modulated radiotherapy (50 Gy/25 fractions) with concurrent fluorouracilbased chemotherapy and then underwent radical surgery 4-8 weeks later.The clinical factors associated with pCR or non-pCR were analyzed by Logistic regression.Results Of the 116 patients,20 (17.2％) achieved a pCR after neoadjuvant chemoradiotherapy.The univariate analysis showed that percentage of circumference of the rectal tube invaded by the tumor,preoperative serum carcinoembryonic antigen (CEA) level,T stage,N stage,distance from the anal verge,degree of tumor differentiation,and maximum tumor diameter were associated with pCR or non-pCR after neoadjuvant chemoradiotherapy for rectal cancer.The multivariate analysis revealed that percentage of circumference of the rectal tube invaded by the tumor,preoperative serum CEA level,and T stage were predictive factors for pCR or non-pCR after neoadjuvant chemoradiotherapy for rectal cancer.Conclusions Non-circumferential tumor (percentage of circumference of the rectal tube invaded by the tumor ＜ 75 ％),low CEA level,and early T stage before treatment may be associated with pCR after neoadjuvant chemoradiotherapy for rectal cancer.

Aged ; Electrocardiography ; Female ; Humans ; Hyperkalemia ; complications ; physiopathology ; Myocardial Ischemia ; etiology

[Objective]To discuss the relationship between partial upper limb edema in the cervical spondylosis patients and the decompression operation.[Method]Ten cervical spondylosis cases with partial upper limb edema were reviewed,including 7 cases of cervical spondylotic myelopathy,2 cases of cervical radiculopathy and 1 case of OPLL(ossification of the posterior longitudinal ligament).Seven cases were treated with subtotal vertebrectomy and anterior internal fixation,one with subtotal vertebrectomy and anterior discectomy,2 cases with posterior decompression and fusion.All the patients were available for follow-up to evaluate the decay of the edema.[Result]All the 10 patients' edema disappeared with the duration from one day to over half a year.The treatment of anterior surgery made patients' edema disappear quicker than that of posterior surgery.[Conclusion]The occurrence of the upper limb edema in the cervical spondylosis patients is related to do with the irritated sympathetic nerve.The edema can be reduced in short term after the decompression operation.

OBJECTIVE:To make clear the present status and developing trend of drugs used in enterprise hospitals and municipal hospitals in Chongqing between2001～2003to offer reference information for the departments concerned.METHO_ DS:The data of drugs used in enterprise and municipal hospitals in Chongqing were retrospectively analysed.RESULTS:In enterprise hospitals and municipal hospitals,the annual increasing rates of sales volumn of drugs were8.24%and18.29%;the anti-infectives accounted for40%～41%and36%～40%of annual sales volumn and sales volumn of imported drugs amounted to4.40%and14.78%of total drugs consumed respectively.CONCLUSION:The sales volumn of drugs in enterprise hospitals is decreasing year by year,while that in municipal hospitals is increasing.

The executive supervision mechanism of IRBs in U.S.A and China are compared and analyzed.Based on the specific situation and realities in China,the plan of adopting good experiences of American peers to perfect the current Chinese IRBs' executive mechanism is also conceived.