Objective To analyze the deep venous thrombosis incentives.Methods The pathogenic factors of 80 cases with deep vein thrombosis were retrospectively analyzed,and risk factors for deep vein thrombosis were explored.Results The incidence of women over the age of 40-70 years old,male age over 60-80 years of age,the incidence rate in women under the age of 50 males,50 females over the age of incidence than men.The incidence of deep venous thrombosis in patients with parts of the left lower limb 55 cases,28 cases of right lower extremity,lower extremity seven cases.80 patients incentives can be found in 76 cases,35 cases of surgery-induced,accounting 43.75％,of which 19 cases of trauma,tumor surgery in 5 cases,10 cases of other diseases surgery;trauma induced 21 cases,accounting for 26.27％ ; higher BP 9 patients,accounting for 11.25 ％ ;4 patients had a history of previous thrombotic,accounting for 5.00％ ;3 cases of infectious diseases,accounting for 3.75 ％ ;4 cases of diabetes,accounting for 5％.There were 4 cases of unexplained,accounting for 5.00％.Conclusion Surgery,trauma,cancer,hypertension,diabetes,infectious diseases and past history of thrombosis are the important risk factors of deep vein thrombosis.Surgery and trauma patients in case of wound healing is not affected,getting out of bed is needed as soon as possible.

let-7e is one member of let-7 miRNA family, a class of endogenous noncoding RNA which consists of 22 nucleotides. The recent miRNA profiles investigation and analysis results showed that let-7e could be involved in embryonic stem cells differentiation, tumorigenesis in cancer, temporal lobe epilepsy and sleep loss, lipopolysaccharide induced inflammation by regulating its target mRNA expression. This paper gives a review of the important biological function of let-7e.

Objective To study the application value of laparoscopic-assisted radical gastrectomy in elderly patients.Methods 114 elderly patients with gastric cancer were randomly divided into observation group and control group.55 patients in the observation group received laparoscopic-assisted radical gastrectomy ,59 patients in the con-trol group received traditional open radical gastrectomy .The operation time,blood loss,postoperative ambulation time, postoperative hospital stay and other information were compared .Results The operative time ,blood loss and postop-erative ambulation ,postoperative hospital stay in the observation group were significantly better than those in the con -trol group(t=12.195,8.562,8.072,10.252,all P<0.05).In the observation group,18 patients had marked effect, effective in 33 cases,ineffective in 4 cases,the total effective rate was 92.7%.In the control group,21 cases had marked effect,effective in 34 cases,ineffective in 4 cases,the total effective rate was 93.2%,the difference between the two groups was not significant (χ2 =0.069,P>0.05).Conclusion The efficacy of laparoscopic-assisted radical gastrectomy is similar with traditional open radical gastrectomy ,and laparoscopic-assisted radicalgastrectomy has ad-vantages of minimally invasive ,faster recovery ,especially suitable for elderly patients with gastric cancer .

Objective To explore the effects of repetitive transcranial magnetic stimulation (rTMS) on hippocampus metabolic changes in rats with temporal lobe epilepsy. Methods A chronic temporal lobe epilepsy model was established by use of lithium-pilocarpine in rats. The hippocampus metabolism of the rats were detected by 1H-MRS method before and after the rTMS and compared with that of the normal controls. Relative ratios of N-acetylaspartate (NAA), choline (Cho), glutamate (Glu) and ?-aminobutyric acid (GABA) over creatine (Cr) were calculated. Results Compared with normal control rats, NAA/Cr and GABA/Cr ratios decreased significantly and Cho/Cr ratios increased significantly in hippocampus of the rats with temporal lobe epilepsy. After rTMS NAA/Cr and GABA/Cr ratios increased significantly and Cho/Cr ratios decreased significantly in hippocampus of rats with temporal lobe epilepsy. However, rTMS did not significantly affect the Glu/Cr ratio in hippocampus of the rats with temporal lobe epilepsy. Conclusion rTMS could improve the hippocampus metabolism and exert a neuroprotective effect against brain damage induced by epilepsy.

OBJECTIVE To investigate clinical status,distribution of the genera,and risk factors of pneumonia caused by fungi in hospitalized senile patients.METHODS The notices of hospitalized senile patients from Jan 2000 to Apr 2003 were analyzed according to Diagnosis Standard of Nosocomial Infection published by National Ministry of Health.RESULTS Totally 135 cases were collected.The isolating ratio of Candida albicans ranked first(54.8%),followed by C.tropicalis(20.0%).The risk factors included underlying diseases,invasive surgical procedure,and prolonged hormones and antibiotics therapy.CONCLUSIONS To prevent and control pneumonia caused by fungi in hospitalized senile patients,the measures such as curing the underlying diseases,reducing invasive surgical procedures,and using antibiotics reasonably should be adopted.

Objective To detect expression of PRKACB protein in non-small cell lung cancer ,thus to study its clinical significance and to analyze the relationship between PRKACB expression and prognosis .Methods We detected the PRKACB protein using immunohistochemistry in 109 lung cancer tissues and corresponding adjacent 20 normal tissues of non-small cell lung cancer patients .Results Compared with normal tissues , the level of PRKACB significantly decreased in lung cancers (χ2 =25.217,P<0.05),and was associated with differentiation (χ2 =9.664,P<0.05),lymph node metastasis(χ2 =4.430,P<0.05),and TNM staging(χ2 =4.430,P<0.05). Furthermore,the low expression of PRKACB protein was correlated with poor prognosis ( overall survival and disease-free survival ) of the patients .Conclusion PRKACB was lowly expressed in non-small cell lung cancer ,and associat-ed with clinical features ,which indicated its important role in clinical diagnosis of non-small cell lung cancer .

Long-term potentiation(LTP)is an important form of synaptic plasticity and an objective indicator to investigate learing and memory synaptic mechanisms.With the development of brain slice technology,more and more experiments associated with LTP are carried out on brain slices,which aim to investigate the mechanism in biology and the change in physiology or biochemistry are carried out on the brain slice.This paper gives an overview of recent advances in research of LTP with technology of brain sliceby suchexamples as follows:The regulated expression mechanisms of long-term potentiation at CA1 synapses,the characteristics of LTP induced in hippcampal slices and its relation with the slice-recovery conditions,the enhancement of the magnitude of early longterm potentiation at CA1 hippocampal synapse by the activation of dopamine receptor,and the enhancement of associative long-term potentiation by the activation of β-adrenergic receptors at CA1 synapses in rat hippocampol slices.

Objective To investigate the prevalence of benign prostatic hyperplasia (BPH) in Beijing. Methods A cross-sectional study was performed at Beijing communities in men aged over 50 years. The International Prostate Symptom Score (IPSS) questionnaire was used to evaluate the symptoms. Transabdominal ultrasonography was used to measure prostate size and the residual urine volume(RUV). Uroflowmetry was performed to record the maximum flow(Q_(max)). One-way ANOVA was used to analysis the differences. Results 1644 subjects were enrolled and the mean age was 64. 5 years (range 50 - 93 years). The mean IPSS, prostate weight andQ_(max) were 9. 9±8. 2, (30. 8± 19.4) and (14. 7 ± 7. 5)ml/s, respectively. They had correlation with age (r=0. 388,0. 262, - 0.371). The prevalence of moderate-severe lower urinary tract symptoms (LUTS) was 50. 8% (835/1644). Prostate volume was greater than 20 ml in 69. 7%(1146/1644) of the subjects.Q_(max) was less than 15 ml/s in 53. 8% (883/1642) of the subjects. Conclusions In men age over 50 years in Beijing, there is positive correlation between LUTS, prostate size and age, and negative correlation between Q_(max) and age. The prevalence of BPH, defined as IPSS greater than 7, maxium flow rate less than 15 ml/s and prostate size greater than 20 ml, is 26. 8%(441/1644).

0.05).DBP and DEHP obviously induced a decrease in organ body weight ratios of testis and epididymis and an increase in organ body weights ratios of liver.Obvious decrease in the sperm counts,spermatozoon survival rate and significant increase in the rate of the sperm deformation were observed.There was synergism between DBP and DEHP on the testis,epididymis and liver organ body weight ratio,as well as sperm counts,sperm survival and deformation rates.Pathological examination showed the seminiferous tubules were irregular shape,degenerative atrophy and interstitial substance broadening,and the seminiferous epithelium were degeneration.Epididymis epithelium was damaged and few of mature sperm was seen.Conclusion DBP combined with DEHP can cause obvious toxic effects on reproductive function in male rats.DBP and DEHP mixture can strongly affect the sperm quantity and quality.Also,some toxic effects to epididymis are observed.

Objective: To investigate the postoperative change of concentration of CD_(41) in peripheral platelet following intra-operative polygeline administration. Method: Sixty-eight patients were randomly divided into studied and control groups. The patients in studied group were infused with 1 000ml polygeline whereas 1 000ml ringer's solution in control group. The concentration of CD_(41) in peripheral platelet was measured with immunocytometry before surgery, one hour, one day, two and five days after surgery. Result: There was no statistical difference in CD_(41) level between the two groups before surgery, CD_(41)values one hour and one day after surgery were obviously decreased in studied group compared with these in control group (P