OBJECTIVE To investigate clinical status,distribution of the genera,and risk factors of pneumonia caused by fungi in hospitalized senile patients.METHODS The notices of hospitalized senile patients from Jan 2000 to Apr 2003 were analyzed according to Diagnosis Standard of Nosocomial Infection published by National Ministry of Health.RESULTS Totally 135 cases were collected.The isolating ratio of Candida albicans ranked first(54.8%),followed by C.tropicalis(20.0%).The risk factors included underlying diseases,invasive surgical procedure,and prolonged hormones and antibiotics therapy.CONCLUSIONS To prevent and control pneumonia caused by fungi in hospitalized senile patients,the measures such as curing the underlying diseases,reducing invasive surgical procedures,and using antibiotics reasonably should be adopted.

let-7e is one member of let-7 miRNA family, a class of endogenous noncoding RNA which consists of 22 nucleotides. The recent miRNA profiles investigation and analysis results showed that let-7e could be involved in embryonic stem cells differentiation, tumorigenesis in cancer, temporal lobe epilepsy and sleep loss, lipopolysaccharide induced inflammation by regulating its target mRNA expression. This paper gives a review of the important biological function of let-7e.

Objective To analyze the deep venous thrombosis incentives.Methods The pathogenic factors of 80 cases with deep vein thrombosis were retrospectively analyzed,and risk factors for deep vein thrombosis were explored.Results The incidence of women over the age of 40-70 years old,male age over 60-80 years of age,the incidence rate in women under the age of 50 males,50 females over the age of incidence than men.The incidence of deep venous thrombosis in patients with parts of the left lower limb 55 cases,28 cases of right lower extremity,lower extremity seven cases.80 patients incentives can be found in 76 cases,35 cases of surgery-induced,accounting 43.75％,of which 19 cases of trauma,tumor surgery in 5 cases,10 cases of other diseases surgery;trauma induced 21 cases,accounting for 26.27％ ; higher BP 9 patients,accounting for 11.25 ％ ;4 patients had a history of previous thrombotic,accounting for 5.00％ ;3 cases of infectious diseases,accounting for 3.75 ％ ;4 cases of diabetes,accounting for 5％.There were 4 cases of unexplained,accounting for 5.00％.Conclusion Surgery,trauma,cancer,hypertension,diabetes,infectious diseases and past history of thrombosis are the important risk factors of deep vein thrombosis.Surgery and trauma patients in case of wound healing is not affected,getting out of bed is needed as soon as possible.

Objective To study the application value of laparoscopic-assisted radical gastrectomy in elderly patients.Methods 114 elderly patients with gastric cancer were randomly divided into observation group and control group.55 patients in the observation group received laparoscopic-assisted radical gastrectomy ,59 patients in the con-trol group received traditional open radical gastrectomy .The operation time,blood loss,postoperative ambulation time, postoperative hospital stay and other information were compared .Results The operative time ,blood loss and postop-erative ambulation ,postoperative hospital stay in the observation group were significantly better than those in the con -trol group(t=12.195,8.562,8.072,10.252,all P<0.05).In the observation group,18 patients had marked effect, effective in 33 cases,ineffective in 4 cases,the total effective rate was 92.7%.In the control group,21 cases had marked effect,effective in 34 cases,ineffective in 4 cases,the total effective rate was 93.2%,the difference between the two groups was not significant (χ2 =0.069,P>0.05).Conclusion The efficacy of laparoscopic-assisted radical gastrectomy is similar with traditional open radical gastrectomy ,and laparoscopic-assisted radicalgastrectomy has ad-vantages of minimally invasive ,faster recovery ,especially suitable for elderly patients with gastric cancer .

Objective To explore the effects of repetitive transcranial magnetic stimulation (rTMS) on hippocampus metabolic changes in rats with temporal lobe epilepsy. Methods A chronic temporal lobe epilepsy model was established by use of lithium-pilocarpine in rats. The hippocampus metabolism of the rats were detected by 1H-MRS method before and after the rTMS and compared with that of the normal controls. Relative ratios of N-acetylaspartate (NAA), choline (Cho), glutamate (Glu) and ?-aminobutyric acid (GABA) over creatine (Cr) were calculated. Results Compared with normal control rats, NAA/Cr and GABA/Cr ratios decreased significantly and Cho/Cr ratios increased significantly in hippocampus of the rats with temporal lobe epilepsy. After rTMS NAA/Cr and GABA/Cr ratios increased significantly and Cho/Cr ratios decreased significantly in hippocampus of rats with temporal lobe epilepsy. However, rTMS did not significantly affect the Glu/Cr ratio in hippocampus of the rats with temporal lobe epilepsy. Conclusion rTMS could improve the hippocampus metabolism and exert a neuroprotective effect against brain damage induced by epilepsy.

Objective To detect expression of PRKACB protein in non-small cell lung cancer ,thus to study its clinical significance and to analyze the relationship between PRKACB expression and prognosis .Methods We detected the PRKACB protein using immunohistochemistry in 109 lung cancer tissues and corresponding adjacent 20 normal tissues of non-small cell lung cancer patients .Results Compared with normal tissues , the level of PRKACB significantly decreased in lung cancers (χ2 =25.217,P<0.05),and was associated with differentiation (χ2 =9.664,P<0.05),lymph node metastasis(χ2 =4.430,P<0.05),and TNM staging(χ2 =4.430,P<0.05). Furthermore,the low expression of PRKACB protein was correlated with poor prognosis ( overall survival and disease-free survival ) of the patients .Conclusion PRKACB was lowly expressed in non-small cell lung cancer ,and associat-ed with clinical features ,which indicated its important role in clinical diagnosis of non-small cell lung cancer .

Objective To evaluate the curative effect of laparoscopic radical gastrectomy combined with Roux-en-Y reconstruction of the digestive tract in treatment of advanced gastric cancer complicated with type 2 diabetes mellitus.Methods 73 advanced gastric cancer complicated with type 2 diabetes mellitus patients between May 2014 to May 2016 underwent distal partial gastrectomy or total gastrectomy with Rouxen-Y GI reconstruction.Results Compared with preoperative data,postoperative level at after 1 month and 3 months were respectively for FPG (14.9 ± 1.9 vs.8.7 ± 0.9,8.8 ± 0.9) mmol/L,OGTT 2 h PG (15.4±1.8 vs.8.68±0.9,8.7 ±0.9) mmol/L,HbA1 (10.4％ ±0.8％ vs.7.4％ ±0.6％,7.4± 0.6),BMI (29.9±1.2vs.25.4±0.7,25.2±0.6) kg/m2,TC (8.4±0.6 vs.6.7±0.6,6.7±0.4) mmol/LandTG (2.53±0.53 vs.1.86±0.27,1.91±0.33) mmol/L(withallP＜0.05).Conclusion Laparoscopic radical gastrectomy combined with Roux-en-Y reconstruction of the digestive tract for advanced gastric cancer significantly improves patients' co-occurring.

Objective To construct eukaryotic cell expression vector of human frangible histone triad (FHIT) gene. Methods A 456 bp cDNA fragment was amplified from the total RNA of normal human thyroid tissue by RT PCR method and cloned into plasmid pcDNA3. The cloned insert was identified by double digestion of the recombinant plasmid with restriction enzymes Kpn Ⅰ and Bst XⅠ and sequenced by Sanger dideoxy mediated chain termination. The expression of FHIT gene was detected by immunocytochemical methods. Results The results showed that the cDNA fragment included 456 bp entire coding region. The recombinant eukaryotic cell expression vector of pcDNA3 FHIT was constructed, and the sequence of the insert was identical to the published sequence. MM96L cells transfected with the pcDNA3 FHIT plasmid expressed high level of Fhit protein in cytoplasm. Conclusion The recombinant plasmid pcDNA3 FHIT can provide a strong molecular tool for the studies of neoplasm pathogenesis.

Objective To study the regulating roles of PAI 2 in the differentiation mechanism of the human epidermis. Methods Human skins were take from the early, middle and late human embryos respectively and observed with immunocytochemistry and in situ hybridization techniques. The cell culture and dot blot were also used in the observation of the materials from late embryo. Results 1 PAI 2 exhibits a very high experssion in the development of embryonic period, with the highest level in the middle embryonic phase while the transcripts of PAI 2 still keep a rather high level in the late human embryonic stage. 2 PAI 2 is mainly localized in the superficial, more differentiatied layers of the epidermis.3 PAI 2 is localizated in peripheral cytoplasm of the vitro or vivo keratinocyte.Conclusion PAI 2 is involved in the regulation of the keratinocyte differentiation. [

By interpreting the connotation of physical factors and its significance in the syndrome differentiation of traditional Chinese medicine (TCM), ideas and methods of the combination of physique differentiation and syndrome differentiation of coronary heart disease (CHD) were proposed. The physique classification research was promoted and standardized. Research methods of physique differentiation were improved. The connotation of relationship between constitution and syndrome was mined. The essence of different syndromes was explored on different syn-dromes of CHD. The physique differentiation, disease differentiation and syndrome differentiation were integrated with the viscera-state theory and syndrome factor differentiation theory. The difference of proteomics technology was used as the means to reveal the pathogenesis of same disease with different syndrome of different CHD constitution. Multidisciplinary crossover study was developed on CHD with constitution theory of TCM and clinical actively, so as to make it better to direct the clinical scientific research practice of CHD.