Complete surgical resection is very important for the outcome of cancer treatment and prognosis.Radionuclide imaging and optical imaging are effective methods for intra-operative guidance of tumor exploration and resection because of their capability in accurate tumor localization and margin delineation.With the development of more specific molecular probes and more sensitive detectors,advanced imaging methods that combine the advantages of both optical and radionuclide imaging,such as Cerenkov luminescence imaging,will be expected to play a more important role as intra-operative navigating agents for surgical oncology in the near future.

OBJECTIVE:To establish the quality criteria of fever and cough syrup for children.METHODS:Thin-layer chromatography(TLC) was employed to make qualitative determination of herbba ephedrae,radix scutellariae,radix glycyrrhi-zae in fever and cough syrup for children,and high performance liquid chromatography(HPLC) was used to determine the con-tent of amygdaloside in its chief medicinal material,almond.RESULTS:The spots in the TLC of herbba ephedrae,radix scute-llariae,radix glycyrrhizae were in the same color with those in the chromatograms of control articles.The linear range of amygdaloside was 0.198?g～4.954?g(r=1.0 000).CONCLUSION:The present method is simple,accurate sensitiveness and highly reproductive,which can be used for the quality control of this preparation.

1 ? 25-dihydroxy-vitamin D3(1? 25 (OH)2 D3)induced differentiation of human promyelocytic leukaemia cells ( HL-60 ) into mature myeloid cells in vitro. The ratio of nuclei to cytoplasma decreased; Their nucleoli reduced; Cells became irrgular and extended the pseudopods. 1 ? 25 (OH ) 2 D3 caused significant increase of nitroblue tetrazolium ( NBT ) reduction and ?-non-specific acid esterase (?-NAE ) and acid phosphatase (ACP ) activities. These data and morphological characteristics suggest that HL-60 cells were conclusively identified as monocytes/macrophages. The histogram of DNA distribution ahylyzed by flow cytometry demonstrated G0 + G1 phase increased and S phase increased and S phase decreased remarkably after treatment with l?, 25 ( OH ) 2 D3 as compared with untreated cells.

MicroRNAs (miRNAs) are a novel class of small noncoding RNAs that regulate gene expression by targeting mRNAs for either cleavage or translational repression.Accumulated studies show that miRNAs aberrantly expressed in tumors are closely related with tumorogenesis and could be potential tumor biomarkers.It will be helpful in the diagnosis,prognosis and(or) predicting treatment response of tumor by imaging miRNAs expression.Several molecular imaging methods,including the optical imaging,radionuclide imaging and multimodality imaging,have been successfully used to visualize miRNAs,miRNAs,a new molecular imaging target,may explore a new field for tumor diagnosis and therapy.

Objective To investigate the effect of high-flux hemodialysis (HFHD) on advanced oxidation protein products (AOPPs) in maintenance hemodialysis (MHD) patients.Methods 40 MHD patients (MHD group),50 non-dialytic chronic kidney disease (CKD) patients (CKD group) and 31 healthy subjects undergoing health examination survey received AOPPs detection.After lowflux hemodialysis (LFHD) for 3 months,HFHD was performed in MHD group for 3 months.Plasma AOPPs and other biochemical indicators were tested at the end of 0,3 and 6 months.AOPPs levels were detected in HFHD and LFHD dialysates.Results Plasma AOPPs level was higher in MHD group than in CKD and control groups,and plasma AOPPs level was higher in CKD group than in control group.Compared with before LFHD in MHD group,AOPPs and triglyceride (TG) levels had no significant differences after LFHD for 3 months(t=0.831,-0.191,both P＞0.05),while plasma cholesterol (CHO) and β2 MG levels were increased in MHD group after LFHD for 3 months(t＝-2.697,10.39,P＜0.05 or 0.001).There were significant differences in plasma AOPPs,TG,β2-MG and CHO levels before versus after HFHD for 3 months (t=4.292,2.271,8.297 and 2.295,P ＜0.001 or 0.05).No AOPPs were detected in HFHD and LFHD dialysates.Conclusions Plasma AOPPs levels are increased in CKD patients.LFHD can not effectively eliminate AOPPs,while long term HFHD can decrease the plasma levels of AOPPs,TG and CHO,and reduce β2-MG accumulation.

Adult ; Femur Head Necrosis ; chemically induced ; drug therapy ; Glucocorticoids ; therapeutic use ; Humans ; Male ; Paraquat ; poisoning

Objective To analyze the clinical characteristics, short-term and long-term outcome, overall survival and prognosis of nasal-type,natural killer T-cell lymphoma(NK/TCL) patients. Methods Clinical data of 45 patients with nasal NK/T cell lymphoma from Jan 2001 to Dec 2008 were analyzed retrospectively. Results The complete remission rate was 53.3% (24/45)and the partial remission rate was 17. 8% (8/45), the overall response rate was 71.1% (32/45). At a median follow-up of 15 months, the 1-year,3-year and 5-year survival rates were 68. 3% ,57. 1% ,and 43. 7% respectively. Multivariate analysis showed that B symptoms was independent prognostic factors of NK/TCL( P = 0. 001 ). Conclusions NK/TCL showed specific characteristics, and the long-term survival rate is still poor. Investigation of optional treatment is needed.

Objective To explore potential related factors of left ventricular hypertrophy in maintenance hemodialy?sis (MHD) patients. Methods Sixty patients were enrolled in the study. Echocardiography was performed to exam LVH in?dexes. There are forty-three patients with LVH (LVH group), and seventeen patients without LVH (non-LVH group). Clini?cal data were collected to exam the levels of hemoglobin (HGB), creatinine (Cr), parathyrin (iPTH), albumin (ALB), triglycer?ide (TG), cholesterol(CHO), calcium(Ca), phosphorus(P), advanced oxygen protein products (AOPP) and C reactive protein (CRP). Results Compared with non-LVH group, age, BP, iPTH, AOPP were higher in LVH group patients while BMI and other biochemistry index does not show any statistical difference. Multiple linear stepwise regressions analysis shows that SBP, iPTH and AOPP are correlated factors to left ventricle mass index (LVMI). Conclusion LVH is prevalent in MHD pa?tients. BP, iPTH and AOPP are the risk factors for LVH.

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Aim: To establish the HPLC and LC-MC methods for the quality analysis of the different species of Radix Paeoniae Alba cultivated in Bozhou. Methods: The LC-MS method was applied to analyze the chemical composition of the cultivated species, the HPLC method was applied to determine the contents of paeoniforin, ben-zoic acid and paeonivayin in crude drugs and processing products. Results: 10 characteristic peaks were identified by LC-MS with reference standards. The significant difference was observed in the chemical compositions of 3 cultivated species. The significant difference was observed in the chemical compositions of 3 cultivated species. The content of paeoniflorin was increased significantly after traditional processing. The results indicated the 4-year-old pubang and 5-year-old xiantiao are the best cultivated species. Conclusion: The methods are simple, rapid and precise to assess and control the quality of different species of Radix Paeoniae Alba cultivated in Bozhou.