Rapid prototyping (RP) technology can be used to translate the CAD model of the product into the physical one, thus the rationalization of the design project and product structure is identified. This paper introduces the basic principle of RP technology and typical prototyping system. The application of RP technology to the development of first-aid kit is also mentioned. RP technology proves effective to reduce the cost and time of the development of medical equipment.

BACKGROUND:How to improve the stability of artificial cornea in the host and reduce the complications is the current key issues to be solved.Therefore,looking for an ideal biocompatible scaffold material is still the focus of the study.OBJECTIVE:To explore the biocompatibility of nano-hydroxyapatite (nHA)/polyvinyl alcohol (PVA) porous composite hydrogel and pure PVA hydrogel as artificial corneal materials.METHODS:Inverted microscope was used to observe cell growth of rabbit corneal stromal fibroblasts when cultured with nHA/PVA composite hydrogel extract or PVA extract for 48 hours.MTT method was used to detect the relative proliferation rate of rabbit corneal stromal fibroblasts cultured with nHA/PVA composite hydrogel extract or PVA extract.Systemic toxicity,allergies,pyrogen reaction and deaths were observed in New Zealand white rabbits at 48 hours after artificial corneal implantation.ELISA and hematoxylin-eosin staining were used to detect changes in serum inflammatory factors at 4 weeks after artificial corneal implantation.Slit-lamp examination was performed to observe corneal or conjunctival hyperemia/edema and corneal neovascularization at 1-4 weeks after corneal implantation.Corneal neovascularization time and neovascularization area were detected after the two materials were implanted.RESULTS AND CONCLUSION:The cells were sparse and grew slowly at 48 hours after culture in nHA/PVA composite hydrogel or PVA extract as compared with the blank control group.The cell growth in the nHA/PVA composite hydrogel group was better than that in the PVA group.The relative cell proliferation rate was significantly decreased at different time after culture in nHA/PVA composite hydrogel or PVA extract compared with the blank control group (P ＜ 0.05),and the relative cell proliferation rate in the nHA/PVA composite hydrogel group was significantly higher than that in the PVA group (P ＜ 0.05).There were two rabbits appearing to have allergic reaction,but no one presenting with pyrogen reaction and death in the nHA/PVA composite hydrogel group;and there were two rabbits appearing to have allergic reaction,and two appearing to have pyrogen reaction,but no death in the PVA group at 48 hours after implantation.The inflammatory factor levels were increased significantly in the nHA/PVA composite hydrogel group and PVA group compared with the control group (P ＜ 0.05),and inflammatory infiltration was milder in the nHA/PVA composite hydrogel group than the PVA group at 4 weeks after implantation (P ＜ 0.05).Corneal neovascularization appeared later in the nHA/PVA composite hydrogel group than the PVA group,and the neovascularization area was also smaller in the nHA/PVA composite hydrogel group than the PVA group (P ＜ 0.05).Taken together,the biocompatibility of nHA/PVA composite hydrogel is superior to that of pure PVA when they are used as artificial cornea materials,which provides a scientific basis for artificial cornea material innovation and extensive clinical application.

Objective To find out evidence for the identification of the aerial roots of Ficus microcarpa Linn. f. and F. altissima Bl.. Methods The fresh aerial roots of the two kinds of plants were collected. The appearance traits were observed by stereoscopy. The velamen surface, cross section of root tip, cross section of the posterior root and powder of two kinds of aerial roots were observed under microscope. Results The number of aerial roots of F. microcarpa Linn. f. was more than that of F. altissima Bl., while the diameter was less than that of F. altissima Bl.. Differences were shown in primary xylems of the transverse section of root tip and posterior root of the two kinds of aerial roots, there were 5-7 vascular bundles in F. microcarpa Linn. f. and 7-10 vascular bundles in F. altissima Bl.. As for the powder, the powder of F. microcarpa Linn. f. was yellowish brown, while that of F. altissima Bl. was reddish brown; F. altissima Bl. had more fibers and longer diameter than F. microcarpa Linn. f. and had cluster crystals in order. Conclusion The appearance and microstructure features showed by the results can be used to distinguish the aerial roots of F. microcarpa Linn. f. and F. altissima Bl..

Objective To analyze the complication rates in 210 patients undergoing transrectal ultrasound-guided 24-core transperineal prostate biopsy.Methods Clinical data of 210 patients who underwent transrectal ultrasound-guided 24-core transperineal prostate biopsy from 2010 to 2012 were collected.A retrospective analysis of complications after prostate biopsy was conducted.Results The main postoperative complications were hematuria,bloody stool,dysuria,urinary retention,fever (＞38℃),urinary infection,vasovagal syncope,and their complication rates were 43.3 ％ (91/210),10.9％ (23/210),23.3％ (49/210),25.2％ (53/210),14.8％ (31/210),21.9％ (46/210),0.48％ (1/210) respectively.In addition,5 patients (2.5％) were hospitalized due to biopsy related complications.Conclusions Transrectal ultrasound guided 24-core transperineal prostate biopsy is an relatively safe and effective puncture method with common minor complications and less severe complications.Adequate attentions should be paid to its complications.

Objective To evaluate the clinical outcomes of a reformed endoscope assisted ureteral stripping technique in post renal transplant patients with renal pelvic tumor of the graft homonymy primitive kidney.Methods Seven post renal transplant patients with renal pelvic tumor of the graft homonymy primitive kidneys(2 males and 5 females)with average age of 54 years old were recruited.Standard retroperitoneal laparoscopic nephrectomies were performed for all patients after placement of a 5 F ureteral stent as the ureteral stripper. After the closure of the ureter at the lower kidney pole level with metal clips, the distal ureter was separated and the ureteral muscle layer and serous membrane layer were split. The ureter muscle layer was then tied tightly to the ureteral stent tip. The ureter and the stent were pulled out through urethra. Transurethral resection around the everted ureteral orifice was performed and the ureter was removed afterwards. The graft function, operation time,complication and estimated blood loss were recorded.Results All the 7 patients successfully underwent the operations and no major complication such as ureteral disruption, stripping embarrassment and converting to open operation happened. The mean operation time was 126 min (ranging from 105 to 160 min) and the mean blood loss was 124 ml (ranging from 80 to 160 ml). Introvesical chemotherapy with farmorubine hydrochloride was performed 3 weeks after surgery. The mean preoperation and 6 months post-operation creatinine and urea nitrogen levels were 136.5μmol/L, 138. 6μmol/L and 7.42 mmol/L, 7.80 mmol/L respectively and there was no statistical difference. There was no tumor recurrence during 6 month follow-up except one case having simultaneous bladder cancer had bladder cancer relapse 3 months after operation and required another TURBt.Conclusion The reformed endoscope assisted ureteral stripping technique is minimally invasive and convenient in the treatment of post renal transplant patients with renal pelvic tumor of the graft homonymy primitive kidney.

Objective To observe the impact of hyperbaric oxygen (HBO) therapy on blood pressure and heart rate in adults with spinal cord injury (SCI). Methods 48 adult SCI patients who accepted HBO therapy from March to November, 2014, were observed. Other 48 non-SCI patients matched sexes and ages were as the controls. Their blood pressure and heart rate were measured before chambering, before compression, HBO for 30 minutes, before decompression and at the end of decompression. The patients were divided into high SCI group and low SCI group based on the injury levels above or below T4. Results The blood pressure was lower in the high SCI patients than their controls (t>6.337, P<0.001), and increased as taking in the oxygen. There was no significant difference between the high SCI patients than their controls as they took in for 30 minutes and before the decompression (P>0.05). No difference was found between both groups in heart rate (P>0.05). For the low SCI patients, neither the blood pressure nor the heart rate was different from their controls (P>0.05). Conclusion The blood pressure may increase during HBO intaking in the high SCI patients, which need be paid attention to.

Objective To investigate effects of laparoscopic repair of abdominal wall incisional hernia using polypropylene and expanded polytetrafluoroethylene(e-PTFE) composite mesh. Methods Forty-one patients with abdominal wall incisional hernia(4~25 cm in length and 3~18 cm in width) were treated in this hospital from October 2004 to August 2005. The patients received laparoscopic mesh herniorrhaphy after complete dissection of adhesion using an ultrasonic scalpel.A polypropylene and(e-PTFE) composite mesh(Bard Composite Mesh) was used and fixed using the Ethicon Endopath Multifeed Stapler(EMS).Results The laparoscopic mesh herniorrhaphy was successfully completed in all the 41 patients without conversions to open surgery.The operative time was 60~182 min(mean,85 min).Postoperatively,the patients felt slight pain and began to take food on the second day.The time to first passing flatus was 25~41 hours(mean,32 hours).The postoperative hospital stay was 5~7 days(mean,6 days).No recurrence occurred during a follow-up period of 6~16 months(mean,9 months) in the 41 patients. Conclusions The laparoscopic incisional hernia repair using the Bard Composite Mesh is a safe and effective method.

Objective To optimize the method for total DNA extraction and RAPD analysis of Nervilia fordii (NF),and to study the genetic diversity of different breeds of NF,the substitute of NF and the fake of NF at molecular level.Methods We used low-pH extraction medium with high salt to extract total DNA,and used randomly amplified polymorphic DNAs (RAPD) to select polymorphism primer from 49 random primers.Twenty-two kinds of NF samples were analyzed by RAPD,and classified by SPSS.Genetic diversity were estimated by Shonnon's index and Nei's index.Results A higher puritiy of DNA can be obtained from fresh HF than that from medical materials.We selected 19 polymorphism primers for the cluster analysis of fresh NF and dried medical material.For medical materials of NF,the distance of amplification band of small-leave breed is close to that of middle-size leave breed,but is far away from that of,big-leave breed and Nervilia plicata.For the fresh materials of NF,there breeds of Nervilia fordii can be classified into one kind,the distance of fresh NF band is far from that of Nervilia plicata,Pachyrhizus erosus and the cultured breed,and more far awary from that of Plantago asiatica and Centella asiatica.Shonnon's genetic diversity is 0.463,Nei's genetic diversity is 0.267.Intra-population genetic variation is obvious compared to iner-population genetic variation.The estimated gene flow from Gst (Nm) is 0.94.Conclusion The molecular difference in different breeds of Nervilia Fordii can be used to identify Nervilia Fordii.The genetic diversity of Nervilia fordii is mainly caused by the geography environment.

Fluorescence in situ hybridization (FISH) is a kind of technique that uses fluoreseently la-beled DNA probes to assess cells for genetic alterations. UroVysion is a technique that uses FISH to detect bladder cancer in the voided urine by four fluorescently labeled DNA probes to the pericentromeric regions of chromosomes 3 ( CEP3 ) ,7 (CEPT), and 17 (CEP17) and to the 9p21 locus ( LSI 9p21 ) location of the p16 tumor suppressor gene. It is reported that sensitivity of FISH was higher than cytology for the detection of all stages and grades of bladder cancer. FDA has approved UroVysion for the detection of recurrent bladder cancer in voided urine specimens from patients with a history of bladder cancer in the year 2001 and from pa-tients with gross or microscopic hematuria but no previous history of bladder cancer in the year 2005. Fur-thermore, UroVysion can benefit the assessment of bladder cancer patients receiving BCG treatment.