Objective To observe the articular cartilage injuries complicated with the rupture of anterior cruciate ligament(ACL)in 21 athletes and 43 non-athletes,and investigate the cause and the pattern of cartilage of knee following the ACL rupture in those patients.Methods The pathologic change,location,degree of cartilage lesion were observed. The relationship between the incidence, occurrent time,degree of cartilage injury and ACL rupture,injured degree of cartilage and the course of ACL rupture were studied.Results The incidence of cartilage injury were 75% in all patients, 66.7% in athletes and 79%in non-athletes. Incidence of cartilage injury in non-athletes was significantly higher than that in athletes (P<0.01).Incidence of serious injury of cartilage in the course more than 1 year was significantly higher than that less than 1 year (P<0.01),but there were no statistical difference between the two groups.Conclusion The incidence of articular cartilage injury following ACL rupture were significantly raised in athletes and non-athletes. The longer the ACL ruptured, the more serious the cartilage injured. Results indicated that articular cartilage injuries in the ACL rupture knee were mainly caused by the instability of knee.

Objective To explore the diagnostic and therapeutic value of arthroscopy for ankle joint sports injury. Methods 52 patients (53 ankles) with ankle sports injury treated by arthroscopy were reviewed retrospectively from December 1992 to December 2001. The modified McGuire Scorting for ankle (1988) was used as efficacy criteria. Results The mean recovery time of daily activities and special sports for the athletes was 10 days and 2.5 months respectively. All of the athletes returned to their previous optimal athletic level except that 1 case recovered slowly due to reinjury. Complications occurred in 3 cases (5 8%). The preoperative score was (60 4?9 1) points and postoperative score (89 0?5 7) points ( t =24 1, P

Objective To study the reasons, patterns of the injury and methods of early reconstruction of the articular cartilage injuries associated with the rupture of anterior cruciate ligament(ACL). Methods The pathologic change, location, degree of articular cartilage lesion were observed in 64 old ACL ruptures and 23 fresh ones by arthroscopy. The relationship between the incidence， duration of the injury, degree of cartilage injury and ACL ruptures, the correlation between the cartilage injury of femoral condyles and meniscus injury were studied. Results The incidence of articular cartilage injury in old ACL rupture group(75％ ) was significantly higher than that in fresh group(26％ )(P

OBJECTIVE To investigate the effect of TiO2 nanoparticles on overcoming cancer multidrug resistance(MDR). METHODS: Doxorubicin-TiO2 nanoparticles(DTN) were prepared, the K562/DOX cells were chosen as the model cells. And doxorubicin solution(F-DOX) and doxorubicin liposomes(DOX-L) were also prepared as the control. The MTT assay were measured, and the amount of doxorubicin in the K562/DOX cells at different time were determined by HPLC. The P-gp expression were detected by flow cytometry. RESULTS: The MTT assay shows that IC50 of group DTN were lower than that of group F-DOX. The uptake test shows that amount of doxorubicin in K562/DOX cells of group DTN was 1.23 times of group DOX-L when in 4 h, and the efflux test shows that amount of doxorubicin in K562/DOX cells was 1.18 times of group DOX-L. The flow cytometry result revealed that the effect of TiO2 nanoparticles on overcoming MDR maybe through down-regulating the expression of P-gp in K562/DOX cells. CONCLUSION: The TiO2 nanoparticles are a new inorganic materials-based nanoparticles which promising approach to overcome MDR.

OBJECTIVE: To investigate the synergetic effect of schisandrin B and liposomes on overcoming multidrug resistance(MDR). METHODS: Co-delivery of doxorubicin and schisandrin B by liposome(DS-L) were prepared, and doxorubicin solution(F-DOX), schisandrin B and doxorubicin mixture(Sch B+DOX), doxorubicin liposomes(D-L), doxorubicin liposomes and schisandrin B mixture(D-L+Sch B), verapamil and doxorubicin mixture(Ver+DOX) were also prepared as the control. The MTT test were measured, and the amount of doxorubicin in the K562/DOX cells at different time were determined, and time course of uptake and efflux were drawn. RESULTS: The MTT test shows that the resistance factor(RF) of group DS-L were 1.68, 14.52 and 1.42 times of group Sch B+DOX, group D-L and group D-L+Sch B respectively. The uptake test shows that amount of doxorubicin of K562/DOX cells in group DS-L was 1.30 and 1.21 times of that in group D-L and group Sch B+DOX respectively. And the efflux test shows DS-L could delay doxorubicin efflux from K562/DOX cells. CONCLUSION: The co-delivery of chemotherapeutics and P-gp inhibitors schisandrin B by liposome is a promising approach to overcome MDR. And there is a synergistic effect between liposome and schisandrin B to overcome MDR.

Multidrug resistance (MDR) is one of the major obstacles for successful chemotherapy in cancer. Inorganic material-based nanoparticles provide a novel choice to effectively circumvent the intrinsic drawbacks of traditional organic materials in overcoming the multidrug resistance (MDR) of cancer cells due to their unique structural and compositional characteristics, for example, high stability, large surface area, tunable compositions, abundant physicochemical multifunctionalities, and specific biological behaviors. In this work, the recent advances of inorganic materials-based nanoparticles to overcome MDR of cancer cells were reviewed. And the advantage and mechanism of inorganic materials-based nanoparticles to overcome MDR were summarized. The recent development of inorganic materials-based nanoparticles (mesoporous SiO2, Au, TiO2, magnetic Fe3O4, Ag, combinations of inorganic materials-based nanoparticles with traditional overcoming MDR strategy etc.), to overcome the MDR were also discussed. And the future developments of these inorganic materials-based nanoparticles are suggested. These elaborately designed inorganic materials-based nanoparticles offer an unprecedented opportunity and show the encouraging bright future for overcoming the MDR of tumors.

There were 48 strains of bacilli obtained from 20 Chinese traditional medicines. Twenty-five strains had antagonistic effect against at least one of ten plant pathogens. Seven strains had antibiosis to more than four pathogens and the best strain had antibiosis to nine pathogens. After physiological and biochemical experiments,eight strains of 25 antagonistic bacilli were proved to be Bacillus subtilis,three were Bacillus cereus,one were Bacillus natto and one were Bacillus licheniformis. At the same time,two kinds of Chinese traditional medicines,which probably had antibacterial effect,were found.

Objective To evaluate the initial clinical application value of placing the Freka R Trelumina guided DSA. Methods The Freka(R) Trelumina was placed guided by DSA in 10 patients with disease of digestive tract post operation complicated with stomal leak or/and stomal stegnosis or various kinds of patients with gastric emptying disorder. Results The Freka(R) Trelumina could be put 20 cm away from Treize anadesma guided by DSA. The achievement ratio was 90%. The putting time was 1 to 3 weeks. The localization of the Freka(R) Trelumina was well. The process of feeding was successfully. The effectiveness of decompression of stomach intestine was also good. Stomal leak healed up quickly. The appearance of gastric emptying disorder disappeared. Conclusion Placing the Freka(R) Trelumina guided by DSA is a simple, safe and reliable method for jejunum nourishment and gastrointestinal decompression.

Objective To investigate the protective effects of left ventricular myocardial perfusion after delivery of acidic fibroblast growth factor (aFGF) in rats with diabetic cardiomyopathy (DCM) by using ultrasound‐targeted microbubble destruction ( UTMD ) with real‐time myocardial contrast echocardiography( RT‐MCE) . Methods Among 64 male SD rats ,52 rats were randomly selected and were induced DCM by streptozotocin through intraperitoneal injecting ,the other rats as normal control group . DCM rats were randomly divided into the DCM model group ,aFGF only group ,SonoVue‐aFGF group and the SonoVue‐aFGF+ UTMD group in this study . The aFGF only group rats were injected with aFGF solution through tail vein ,the SonoVue‐aFGF group were injected with SonoVue‐aFGF solution through tail vein and SonoVue‐aFGF+ UTMD group rats were injected with SonoVue‐aFGF solution through tail vein and using UTMD simultaneously . All rats underwent conventional echocardiography and RT‐MCE exams before and 4 weeks after intervention . Left ventricular ejection fraction ( LVEF) and left ventricular fraction shortening( LVFS ) were measured by conventional echocardiography . The plateau intensity ( A ) ,initial slope of the curve (β) and myocardial blood flow ( A ×β) of left ventricular anterior wall at the papillary muscle level were measured in left ventricular short‐axis view by RT‐MCE . Results Before intervention , LVEF and LVFS in the DCM model group ,aFGF only group ,SonoVue‐aFGF group and the SonoVue‐aFGF+UTMD group were significantly lower than in the normal control group ( P <0 .01) .During 4 weeks after intervention ,LVEF and LVFS in the aFGF only group ,SonoVue‐aFGF group and the SonoVue‐aFGF+UTMD group were slightly increased than those in the DCM model group ,but no statistically significant differences were found ( P > 0 .05) ,however A and A × β in the SonoVue‐aFGF+ UTMD group were significantly increased than those in the DCM model group( P < 0 .01) . Compared with the same group before intervention ,A and A ×βin the SonoVue‐aFGF+UTMD group were higher ( P <0 .05) and these in the DCM model group were lower during four weeks after intervention ( P < 0 .05) . Conclusions Acidic fibroblast growth factor delivered by using UTMD can improve the left ventricular myocardial perfusion in diabetic cardiomyopathy rats .

AIM: To investigate the effect of problem - based learning ( PBL) used in the teaching of medical students'evidence-based medicine ( EBM) .
METHODS: Five classes ( total 147 students ) were randomly selected as experimental ( PBL ) group, at the same time, another 5 classes ( total 149 students ) were also randomly selected as control group, using traditional teaching method ( lecture-based learning, LBL ) in 2010 grade. The final examination scores of the experimental group were compared with control at the end of term. In addition, all students were interviewed using self -administered questionnaire to obtain their evaluation for PBL practice. SPSS13. 0 software was used for statistical analysis.
RESULTS: The homogeneity test in baseline survey showed that the basic characteristics between the two groups of students were no significant differences, and were comparable (P>0. 05). Final exam results showed that in addition to the scores of the EBM basic knowledge indicated no significant difference between two groups of students (P>0. 05), for the 5 steps of EBM procedure, namely, asking questions, finding the best evidence, evaluating the evidence, using and practicing the evidence, re - evaluating the evidence, and the total scores between the two groups, there were significant statistically differences (P<0. 05). The results to student learning evaluation showed that there was no significant difference between the two groups (P>0. 05) in aspects of better understanding classroom knowledge, improving language expression ability, and writing skill exercises. And other residual items had a significant difference ( P<0. 05), especially in aspects of improving enthusiasm for learning, self - study ability, improving learning efficiency, information analysis and utilization ability, team collaboration, and communication between teachers and students, however, there was a very significant difference (P<0. 001) between the two groups.
CONCLUSION: PBL teaching mode can effectively improve teaching effectiveness and the quality of EBM teaching, so the this teaching mode is worth further popularizing.