Humans ; Osteonecrosis ; complications ; diagnosis ; Otitis Externa ; complications ; diagnosis ; Temporomandibular Joint ; pathology

Objective To make a preliminary study on self-perception of profile among orthodontic patients.Methods A total of 226 orthodontics patients (79 males and 147 females with average age of 19.3 years) were enrolled in this study.All the patients were treated in the Department of Orthodontics,Sun Yat-sen University Hospital of Stomatology during May to August 2016.Self-perception of profile was investigated in the patients.They were asked whether they had ever noticed their own profile and to choose from among various photos the one that most resembled their own profile.Then profile photos of patients were taken and measured.Differences between self-perception profile and actual profile were compared using paired t test.Results There were 147 patients (41 males and 106 females) answered that they had noticed their own profile.Difference was significant between patients' actual profile and self-perception profile (P＜0.05).Difference was significant between male patients' actual profile and self-perception profile (P ＜0.05).Difference was not significant between female patients' actual profile and self-perception profile (P ＞ 0.05).Compared with male patients,difference was smaller between female patients' actual profile and self-perception profile.Conclusions Compared with male patients,female patients put more emphasis on their own profile.Patients' self-perception of their own profile is not accurate.Female patients' self-perception of their own profile is more accurate than male patients.

BACKGROUNDThe multilineage differentiation potential ability of bone marrow stromal cells (BMSCs) showed great potential in tissue engineering, while vascular endothelial growth factor 165 (VEGF165) promotes vasculogenesis and further promotes tissue regeneration. This study aimed to assess the ability of rat BMSCs expressing human VEGF A165 (hVEGF165) to promote tissue repair in rat model of radiation-induced injury.

METHODSRat BMSCs were isolated from the tibia. Plasmid DNA expressing hVEGF165 was stably transfected into BMSCs using liposomes. The right hindlimb muscle of 40 rats was irradiated using a (60)Co γ source (total dose 30 Gy). The animals were divided into four groups (n = 10): not injected with BMSCs (control; group 1) or intramuscularly injected two times (once in 2 weeks) with pcDNA(TM)3.1-transfected BMSCs (group 2), untransfected BMSCs (group 3), or hVEGF165-transfected BMSCs (group 4). Angiography was performed 1 week after the last injection of BMSCs; samples of the hindlimb muscle were subjected to transmission electron microscopy, ultrastructural analysis, reverse transcription-PCR (RT-PCR), Western blotting, and immunohistochemistry.

RESULTSRat BMSCs with multipotent differentiation capacity were isolated. hVEGF165-transfected BMSCs overexpressed hVEGF165 mRNA and protein. Injection of BMSCs (groups 2-4) increased the average vessel number, density, diameter, and cross-sectional area; mRNA expression of the myogenic markers including myoblast determination protein, myogenin, and a-smooth muscle actin; and CD31 protein expression; and promoted the repair of blood vessels and myofibers after radiation-induced injury compared to group 1; each of these parameters and hVEGF165 mRNA or protein expression were markedly improved in rats injected with hVEGF165-transfected BMSCs compared to groups 2 and 3.

CONCLUSIONSBMSCs expressing hVEGF165 enhanced the repair of radiation-induced tissue injury by promoting vasculogenesis and muscle fiber regeneration. BMSCs expressing hVEGF165 may have potential clinical applications.

Animals ; Bone Marrow Cells ; metabolism ; physiology ; Cells, Cultured ; Humans ; Mesenchymal Stromal Cells ; metabolism ; physiology ; Microscopy, Electron, Transmission ; Osteogenesis ; genetics ; physiology ; Radiation Injuries ; therapy ; Rats ; Vascular Endothelial Growth Factor A ; genetics ; metabolism