No abstract available.
Thyroglobulin*

No abstract available.
Thyroglobulin* ; Thyroid Gland*

No abstract available.
Thyroglobulin* ; Thyroid Gland*

The human thyroglobulin (hTG) level was quantified in 31 serum samples taken from healthy people who aged from 18-55 years old. The standard curve, accuracy of technique and the serum hTG level of healthy people was determined. The results showed that the hTG quantified technique using immunoradiometric assay (IRMA) kits of CIS Bio International provided high accuracy. The average serum hTG level of healthy people is 7.35+/-6.99ng/ml; with common rage is 0.00 to 2ng/ml.
Thyroglobulin ; Immunoradiometric Assay

The quantification of thyroid oringinated hormone and thyroglobulin by immunoradiometry assay (IRMA) in healthy people (newborn to elderly) and patients with thyroid diseases has shown that T3,T4 and TSH in healthy people changed clearly with age (maximal level after birth 24 hours and reduction after birth 72 hours. T3 and T4 were increased but TSH was reduced in patient with basedow when compared with these in healthy people. In the contrary, T3 and T4 were reduced and TSH was increased obviously in patients with hypothyroidism comparing with the healthy people.
Thyroid Diseases ; Thyroglobulin

This study aimed to investigate the thyroglobulin tablet formulation produced from hog thyroid glands. This formulation were designed and optimized by MODDE 5.0. High performance liquid chromatogaphy (HPLC) was used to measure thyroid hormone concentration in the thyroglobulin tablets. The hormone in thyroglobulin tablets have got required stability after accelerated test at the temperature of 40 + 2oC and the relative humidity of 75 + 5% for 3 months
Thyroglobulin ; Tablets ; Pharmaceutical Preparations

This study related to a process for the production of thyroglobulin from hog thyroid glands. Ground hog thyroid glands were subjected to the saline digestion to produce a thyroglobulin extract. The extract was precipitated by pH adjustment and then heated to a denaturing temperature. The exctract process was designed and was optimized by MODDE 5.0. Using high performance liquid chromatogaphy (HPLC) to measure thyroid hormon concentration in the thyroglobulin. The product had a T4 to T3 ratio is 5. The hormon of the thyroglobulin have got required stability after accelerated test at the temperature of 40 + 2oC and the relative humidity of 75 + 5% for 3 months
Thyroglobulin ; Thyroid Gland

Healthy normal adult volunteers without endocrinological diseases, aged 18-45 and menopausal subjects (>45 years old of age) diagnosed definitly as osteoporosis, Results showed: blood level of calcitonin in healthy adults, male subjects with n=30, median 75, min.30, max 125pg/ml female subjects n=30, median 30, min 10, max 50pg/ml respectively. Calcitonin in male is higher than in female (p<0,05). In female subjects: menopausal patients with osteoporosis, calcitonin level is lower than normal woman in reproductive age (p<0,01). Thyroglobuline level in healthy adult is 13,58 4,2 ng/ml, with no cosiderable difference between male and female
Osteoporosis ; Menopause ; Blood ; Thyroglobulin ; Calcitonin

BACKGROUND: Serum thyroglobulin(Tg) is a valuable and sensitive tool needed in the follow-up of patients with differentiated thyroid cancer(DTC), but antithyroglobulin antibody(Anti-Tg), common in patients with DTC, can interfere with the assay for Tg. In this study, we evaluated the influence of Anti-Tg on the measurement of Tg using the immunoradiometric assay(IRMA). METHODS: In using ELSA-hTg in vivo test(CIS international, Schering, France), a solid phase two-site IRMA was used to measure Tg(23.5ng/mL, 62.5ng/mL) under the absence or presence of three concentrations of Anti-Tg(25U/mL, 50U/mL, 100U/mL). We also performed Tg measurement using patients serum that was mixed with patients serum containing high Anti-Tg. ANOVA and Scheffe tests were performed to evaluate the effect of Anti-Tg on Tg IRMA, and an inverse regression was made to calculate the level of Tg from measured Tg and used Anti-Tg levels and also to assess the degree of effect of anti-Tg on Tg IRMA. RESULTS: In measuring Tg using the standard solution, the presence of Anti-Tg resulted in a falsely suppressed Tg value. The IRMAs for 23.5ng/mL of the standard Tg solution resulted in 24.5+/-.1 ng/mL under no Anti-Tg, 11.8+/-.4ng/mL under 25U/mL of Anti-Tg, 7.7+/-.1ng/mL under 50U/mL of Anti-Tg, and 4.5+/-.4ng/mL under 100U/mL of Anti-Tg. IRMAs 62.5ng/mL of the standard Tg solution resulted in 65.9+/-.7ng/mL under no Anti-Tg, 36.3+/-.2ng/mL under 25U/mL of Anti-Tg, 23.7+/-.7ng/mL under 50U/mL of Anti-Tg, and 14.0+/-.0ng/mL under 100U/mL of Anti-Tg. (ANOVA test, p=0.000). The degree of suppression of the measured Tg value was positively correlated with the Anti-Tg level (Quadratic model regression, Sig T=0.000). The presence of Anti-Tg also resulted in a falsely suppressed Tg value for the Tg measurement using patient's serum. CONCLUSION: The presence of Anti-Tg could consist of the use of Tg as a tumor, therefore Anti-Tg should be measured in all patients diagnosed with DTC. The interpretation of the Tg level must be performed with extreme caution in patients with Anti-Tg.
Humans ; Immunoradiometric Assay* ; Thyroglobulin* ; Thyroid Gland

PURPOSE: Periods of L-T4 withdrawal and low iodine diet, which are required prior to the treatment and tracking tests that take place after a thyroidectomy, can be of a long duration and cause suffering for patients. The purpose of this study, conducted in South Korea, was to confirm if periods of L-T4 withdrawal and low iodine diet can be shortened by using TSH level prediction. By inquiring into the correlation between TSH level and serum Tg level, and measurement of the amount of iodine present in urine during the low iodine diet period, a thyroglobulin (Tg) cutoff level can be predicted. METHODS: total of 168 patients were included as research subjects. In each case, L-T4 was suspended 3-4 weeks prior to conducting radioactive iodine ablation and 131I scan, and then a low iodine diet was carried out for 2-4 weeks. Serum TSH, Tg and anti-Tg antibodies were measured on the second week of L-T4 withdrawal, and the spot urine Iodine/Creatinine ratio was measured on the second and fourth week after treatment. RESULTS: Three weeks after L-T4 withdrawal, TSH levels increased to over 30μIU/ml in 97.2% of the patients, and serum thyroglobulin levels also increased with TSH level to over 30μIU/ml. There was no measured difference in the amount of iodine in the subject's urine during the low iodine diet period. CONCLUSION: It was found that L-T4 withdrawal can be reduced to 3 weeks or less, and that the Tg cutoff level differs according to TSH level. Based on these results, we suggested that the low iodine diet period can be reduced to 1-2 weeks.
Antibodies ; Diet ; Humans ; Iodine* ; Korea ; Research Subjects ; Thyroglobulin* ; Thyroidectomy ; Thyrotropin*