Study on the erythrocyte metheglobine reductase activity in 30 patients with ages of 15 - 50 and essential marrow failure in B¹ch Mai hospital, 30 healthy men and 30 healthy women with ages of 17-50 and full standard of blood donor in the Institute of Hematology and Blood Transfusion. The criteria for diagnosis of marrow failure comprised medical history, clinical and hematological features. The results have shown that the metheglobine reductase activity in men was higher than this in women in the both two diseases and health groups. This enzyme activity in the patients with anemia due to marrow failure was higher 1.8 times than this in healthy people.
Oxidoreductases ; Erythrocytes ; anemia ; Hematologic Diseases ; Bone Marrow Diseases

The high level of Methemoglobinemia influences the combination of blood with oxygen and oxygen transfer to cells which can lead to the life in the normal condition. Methemoglobine is a component of erythrocyte in a very small amount(less than 1% of total Hemoglobine). An increased Methemoglobine leads to the disorder of oxygen delivery. The clinical symptoms are direct proportional with the methemoglobinemia level. The hypermethmoglobinemia includes the congenital- methemoglobinemia and the acquired methemoglobinemia. The congenital hypermethemoglobinemia attibuted to a partly or completed deficiency of Methemoglobine reductase in patients with the deficiency of genetic heterozygous - enzyme or abnormal methemoglobine: the acquired methemoglobinemia occurred after exposure with the chromatic compound, food, water with much nitrate or use of some drugs. The quantitative analysis of methemoglobinemia has a values in the diagnosis, treatment and prognosis
Methemoglobinemia ; Prognosis

The hyper-methemoglobinemia causes the oxygen transfer disorder. The clinical symptoms depends on the level of methemoylobinemia. Hypermethemoglobinemia can be congenital or acquired hypermethemoglobinemia. The congenital hypermethemoglobinemia caused by partial or total methemoglobine reeducates deficiency in patients with rare genetic heterozygous enzyme deficiency due to the abnormal hemoglobin. The acquired hypermethemoglobinemia occurred after exposure to colour compound, food and drinking water with nitrate or drugs. The determination of causative agent of hypermethemoglobin help treating more effectively
Methemoglobin ; Hemoglobins, Abnormal ; Therapeutics ; Methemoglobinemia

3 nitrogene derivatives had been synthetized successfully from 4’,5,6-trihydroxy 3,3’,7-methoxyflavone (chrysosplenol C) isolated from Miliusa balansae Fin.& Gagnep. 10 derivatives of chrysosplenol C were prepared. These 13 compounds were tested for cytotoxicity against Hepatoma-G2. Among them, 4 showed moderate activity (IC50<5microgram/ml )
Nitrogen ; chemical synthesis ; analogs & derivatives ;

Some enzymes of key in the metablism of red blood cell in human, rabbit and rat were studied. In these subjects, glucose, 6-phosphate dehydrogenase, aldolase, pyruvat kinase, methemoglobin activities were particulary but associated with one to other. In human red blood cell, G6PD, ALD, PK and MHR were 4.84  0.94; 65.58  31.60; 226.14  47.29 and 3.27  0.52 respectively. In rabbit, they were 7.61  00.4; 79.77  43.5; 164.62  61.9; 5.25  1.09, respectively and in rat: 23.17  2.18; 89.73  18.0; 49.9 30.23 and 3.9  0.58, respectively.
Enzymes ; Erythrocytes ; Humans ; Rabbits ; Rodentia

Background: There are many factors to assess face morphology and standard occlusion, in which sagittal relationship index is an important one. This index in cephalometric film can help to study developmental changes, to evaluate structures of bone and soft tissues, and to make decisions in orthodontic treatments. Objectives: Determination of the sagittal relationship index on cephalometric film in Vietnamese citizens aged 18 to 19 years. Subjects: 100 Vietnamese (42 men and 58 women) aged 18 to 19 years, who had 28 healthy and natural teeth. Method: Descriptive cross-sectional study. All participants were X-rayed on cephalometric film and then special points, angles, and planes were defined on these films. Data was analyzed by using one-way ANOVA test, t - Student test in Epi-Info software version 6.04 and SPSS version 10.0. Results and conclusion: Some sagittal craniofacial indexes on cephalometric film were defined as followed: in both sexes, mean SNA (degree) = 81.99 +/- 3.77 (82.81+/-4.40 in men, 81.40+/-3.16 in women, p> 0.05); mean SNB = 79.66 +/- 4.10 (82.81+/-4.36 in men, 78.40+/-3.40 in women, p< 0.05); mean ANB = 2.33 (1.40 in men, 3.00 in women, p< 0.05); mean l/SN = 109.36 +/- 6.46 (110.55+/-7.77 in men, 108.49+/-5.22 in women, p> 0.05); mean 1/ MP = 95.85 +/- 6.86(93.68+/-7.27 in men, 108.49+/-6.15 in women, p> 0.05); mean 1/1 = 120.02 +/- 13.06 (125.42+/-9.08 in men, 116.10+/-14.13 in women, p< 0.05). These findings showed that the Vietnamese populaces have alveolar protrusive tendency.
Sagittal relationship index ; cephalometric film

Background: The intracranial hemorrhage (lCH) in infant due to vitamin K deficiency were been informed a lot at all over the world since the year of 80's. In Viet Nam, in recent years, this disease still most occurs which account for 110-130 children/100000 alive infant. The intracranial hemorrhage (lCH) in infant causes severe neurological, psychiatric sequelae. Objectives: This study aims to analyze the characteristics of epilepsy, changing of electroencephalogram and lesions of brain after intracranial hemorrhage in infant. Subjects and method:A descriptive, cross sectional study was conducted on 97 infant patients with the age ranged from 8 days to 3 months years old whom treated in National Hospital for Peadiatric. Results: Epilepsy was common (45.3%), with generalized seizures in 24.7% , partial seizures in 20.6%. The main intracranial lesions were cerebral cyst and atrophy in right or left hemisphere or both sides. Electroencephalic activity was found impaired in most of patients: Disorder of background waves in 19.6 %, low localized waves in 31%, proxystic ativity with spike in 45.3% and decrease in electroencephalographic amplitude in cystic areas. Conclusion: The epilepsy, abnormal electroencephalographic activities, severe lesions of brain were common after intracranial hemorrhage in infant.
Intracranial Hemorrhages/ diagnosis ; pathology ; therapy ; Infant

Background: \r\n', u'Japanese encephalitis virus (JE) is the most prevalent in Asia, Pacific and in mainland northern Australia; and considered to be the leading cause to the acute encephalitis. The case- fatality and sequela rates in children stay rather high. There were some medical technologies for the JE diagnosis, of which is the application of the expensive MAC- ELISA and PEN TAX kits.\r\n', u'Objectives: \r\n', u'To evaluate and compare the light sensitivity of MAC- ELISA and PENTAX Kits in JE diagnosis.\r\n', u'Subjects and method: \r\n', u'48 pairs of sera samples obtained from the patients with clinically manifested Japanese encephalitis (JE) in 2003- 2005, in some northern provinces, they were applied and tested into MAC- ELISA Kits.\r\n', u'Results:\r\n', u'The sensitivity of MAC- ELISA and PENTAX kits detecting IgM against JE virus were 95.71%, and 98.57%, respectively. In addition to that, the sensitivity of these two kits used to detect JE IgM within the first 7 days of the disease was very high (around 92.31%-96.15%).\r\n', u'Conclusion: \r\n', u'The sensitivity of MAC- ELISA and PENTAX kits used to detect IgM against JE virus was very high. \r\n', u'
Encephalitis ; Japanese/ diagnosis

Dravet syndrome is a rare and catastrophic type of epilepsy in infants. Acute encephalopathy has been sporadically reported in patients with Dravet syndrome; however, the risk factors for this serious complication have not been identified. We report two patients with a clinical diagnosis of Dravet syndrome who experienced acute encephalopathy initiated by refractory status epilepticus. SCN1A mutational analysis revealed a previously reported nonsense mutation in one patient and a novel missense mutation in the other. Analysis of our cases and previously published cases revealed that patients with Dravet syndrome who have a more severe phenotype have an increased likelihood of developing acute encephalopathy compared with patients with less severe phenotypes.
Epilepsies, Myoclonic

Background: Recently Japanese Encephalitis (JE) virus type 1 has surfaced and is co-circulated with JE virus type 3 in the northern areas of Viet Nam, so a sensitivity of JE viral antigen genotype 3 to detect IgM is required. Objectives: To compare the sensitivity of JE viral antigen genotype 1 and 3 to detect IgM against the JE virus. Materials and method: 783 cerebrospinal fluid and serum samples from viral encephalitis cases from 1999-2005 were collected and examined by MAC-ELISA for JE viral antigen genotype 1 and 3. Results: The agreement on the diagnosis of these kinds of antigen was 99.7% and the sensitivity of JE viral antigen genotype 3 was higher than that of genotype 1. Thus, JE viral antigen genotype 3 could be considered as the selected antigen for JE diagnosis in Viet Nam. IgM titer determined by JE viral antigen genotype 1 was higher than that of genotype 3 in 2003 and 2005 and lower in 1999, 2000, 2001, 2002 and 2004. Conclusion: The dominant phenomenon of JE viral genotypes differing over the years might be due to the interaction of the virus and its vectors. Further study is required to clarify this observation.
Japanese Encephalitis ; antigen