1. In vitro and in vivo trypanocidal action of aescin and aescin liposomes against Trypanosoma evansi in experimental mice
Matheus Dellaméa BALDISSERA ; Thirssa Helena GRANDO ; Carine Eloise PRESTES ZIMMERMAN ; Janio Morais SANTURIO ; Silvia Gonzalez MONTEIRO ; Matheus Dellaméa BALDISSERA ; Nathieli Bianchin BOTTARI ; Roberto Christ VIANNA SANTOS ; Roberto Christ VIANNA SANTOS ; Ana Júlia FIGUEIRÓ DALCIN ; Patrícia GOMES ; Renata Platcheck RAFFIN ; Aleksandro Schafer DA SILVA
Asian Pacific Journal of Tropical Biomedicine 2014;4(12):947-951
Objective: To verify the trypanocidal effectiveness of aescin and aescin liposomes against Trypanosoma evansi in vitro and in vivo. Methods: Aescin and aescin liposomes were used in vitro on trypomastigotes at different concentrations (0.5%, 1.0% and 2.0%) and exposure times (0, 1, 3, 6 and 9 h). In vivo tests were performed using mice as the experimental model. Trypanosome evansi infected mice were treated with aescin and aescin liposomes with doses of 60 and 100 mg/kg during 4 d. Results: The three concentrations tested in free form and nanoencapsulated showed trypanocidal activity in vitro, completely eliminating the parasites in small concentration after 6 h of assay. Animals treated with aescin (100 mg/kg) and aescin liposomes (100 mg/kg) showed increase in longevity, however without curative effect. Conclusions: Active compounds present in natural products, such as aescin, may potentiate the treatment of trypanosomosis when used in association with other trypanocidal drugs.
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