No abstract available.
Amniotic Fluid* ; Female ; Humans ; Thiram*

PURPOSE: An attempt was made to determine if the pre-LASIK operative BAC-STAT LASIK Ring plays a role in reducing postoperative infection. METHODS: Candidates for this study were 72 eyes of 36 patients, scheduled to undergo LASIK operation. Eyes were divided into two groups: right eyes with preoperative BAC-STAT bacteriostatic LASIK Ring (BAC-STAT LASIK Ring: American Optisurgical Inc., Lake Forest, California, U.S.A.) and one minute of irrigation (experimental group), and left eyes with only preoperative BSS irrigation (control group). The authors tried to demonstrate a difference of identified pathogens between the two groups after a growth of bulboconjunctival lesion in blood agar plate (BAP), MacConkey agar plate (MCA), Thioglycolate medium broth (TG), and Ogawa egg medium. RESULTS: Among the patients receiving only BSS irrigation, pathogens were identified in eight eyes before surgery and in four eyes after surgery in BAP and MCA medium. Among the patients receiving BAC-STAT LASIK Ring and irrigation, pathogens were identified in nine eyes before surgery and in three eyes after surgery in BAP and MCA culture medium. In addition, among the control population, pathogens were found in 21 eyes both before and after surgery in TG culture medium. However, among the experimental population, pathogens were grown in 23 eyes before surgery but in only 14 eyes after surgery. No growth of pathogens was reported in all patients before and after surgery in a 4-week Ogawa egg culture medium. CONCLUSIONS: This study suggests that preoperative BAC-STAT LASIK Ring insertion followed by irrigation decreases the incidence of postoperative infection.
Agar ; California ; Humans ; Incidence ; Keratomileusis, Laser In Situ* ; Lakes ; Ovum ; Thiram

Benzoyl peroxide has been known to have bacteriostatic activity against Corynebacterium acnes and to have comedolytic action in patients with acne vulgaris. The present study was undertaken to evaluate the therapeutic effect of Renoxal, a 5% benzoyl peroxide lotion, in patients with acne vulgaris. A total of 35 patients entered this study at the Department of Dermatology, Seoul National University Hospital during the five months period from March to July, 1978. All patients were instructed to apply Benoxal on their affected sites once or twice daily; and the number of lesions on three fixed areas were counted before and every week for four weeks after the treatrnent. Two female patients stopped the application due to developrnent of contact dermatitis to Benoxal The results observed in the 33 patients were as follows: 1. The percentage decrease in total number of lesions in four weeks after treatment was 51.1% 2. Closed and open comedones were gradually eliminated; and their numbers were decreased in four weeks after treatment by 45. 8% and 55. 3% respectively. The papules were decreased. by 59. 1 @2 after the first week of treatment, but showed. a transient lag around tbe second week and then marked 61. 5% in four weeks after treatment. 3. Observed side effects included burning sensation,(3 cases), tightness (3 cases), itching sensation (2 cases) and scaling (1 case). All were tolerable without any specific measure or discontinuance of the application. The authors concluded througb this experiment that Benoxal (5% benzoyl peroxide lotion) is a very effective local therapeutic agent in the treatment of acne vulgaris patients.
Acne Vulgaris* ; Benzoyl Peroxide ; Burns ; Dermatitis, Contact ; Dermatology ; Female ; Humans ; Propionibacterium acnes ; Pruritus ; Sensation ; Seoul ; Thiram

BACKGROUND: Because there is difficulty in the addition of known preservatives to oil in water emulsion such as propofol, ethylenediaminetetraacetic acid (EDTA) added to this may formulate for the antimicrobial activity; however, this formulation has side effects such as hyperlipidemia and pain on injection. We have developed a newly formulated poloxamer-solutol propofol which is considered to be free from hyperlipidemia. The aim of this study was to evaluate the possibility of bacterial growth in poloxamer-solutol formulated propofol compared to original propofol and EDTA added propofol. METHODS: Broth cultures (100nl) of four standard preservative efficacy test organisms (Staphylococcus Aureus, Pseudomonas Aeruginosa, Escherichia Coli, Candida Albicans) were added to 9.9 ml of four test formulations. Subjected formulations were original propofol (AstraZeneca Co, 1% solution, UK), EDTA added propofol (0.0055% EDTA added propofol), Poloxamer-Solutol formulated propofol (poloxamer 188/407 and solutol mixture), and normal saline at approximately 200 colony forming units/ml. The test formulations were incubated at 25degreesC and 32.5degreesC (Tryptic soy agar medium for bacteria and Sabrouraud dextrose agar medium for fungus) and tested for viable counts after 24 and 48 hours. RESULTS: Original propofol supported the growth of all microorganisms at both temperature and time. EDTA added propofol inhibited the growth of microorganisms more than the original propofol, but not so much as the poloxamer-solutol formulated propofol. Saline showed a similar pattern as EDTA added propofol. CONCLUSIONS: Poloxamer-solutol formulated propofol possesses more bacteriostatic activity against all four microorganisms than the original and EDTA added propofol.
Agar ; Bacteria ; Candida ; Edetic Acid* ; Escherichia coli ; Glucose ; Hyperlipidemias ; Propofol* ; Pseudomonas aeruginosa ; Thiram

The authors carried out the cystoplasty by regeneration to a 24 year-old female of tuberculous contracted bladder with artificial bladder. The artificial bladder was prepared by using nobecutane-sprayed paper, which was designed by repetition of Nobecutane spraying and drying. The artificial bladder was subjected to a crucial incision on the dome and a No. 10 porous catheter was inserted from the urethra into the bladder. The artificial bladder was sutured with the original incised one with a margin of 1 Cm using 3-0 chromic catgut. The authors observed for 2 months postoperatively. The following results were obtained. 1. The artificial bladder fell off completely in the bladder in 34 days after the cystoplasty, which was extirpated transurethrally. 2. The bladder capacity was about 30 cc before the operation. Following the cystoplasty, it increased up to 180-220 cc by the 50th postoperative day, and the frequency of urination was 8 times in day and 3 times in night.
Catgut ; Catheters ; Female ; Humans ; Regeneration ; Thiram ; Urethra ; Urinary Bladder* ; Urination ; Young Adult

The purpose of this study was to provide a basis for studying the molecular mechanism of pharmacological action of chlorhexidine digluconate. Large unilamellar vesicles (OPGTL) were prepared with total lipids extracted from cultured Porphyromonas gingivalis outer membranes (OPG). The anthroyloxy probes were located at a graded series of depths inside a membrane, depending on its substitution position (n) in the aliphatic chain. Fluorescence polarization of n- (9-anthroyloxy)stearic acid was used to examine effects of chlorhexidine digluconate on differential rotational mobility, while changing the probes' substitution position (n) in the membrane phospholipids aliphatic chain. Magnitude of the rotational mobility of the intact six membrane components differed depending on the substitution position in the descending order of 16- (9-anthroyloxy)palmitic acid (16-AP), 12, 9, 6, 3 and 2- (9-anthroyloxy)stearic acid (12-AS, 9-AS, 6-AS, 3-AS and 2-AS). Chlorhexidine digluconate increased in a dose-dependent manner the rate of rotational mobility of hydrocarbon interior of the OPGTL prepared with total lipids extracted from cultured OPG, but decreased the mobility of membrane interface of the OPGTL. Disordering or ordering effects of chlorhexidine digluconate on membrane lipids may be responsible for some, but not all of its bacteriostatic and bactericidal actions.
Chlorhexidine* ; Fluorescence Polarization ; Liposomes ; Membrane Lipids ; Membranes* ; Phospholipids ; Porphyromonas gingivalis* ; Porphyromonas* ; Thiram ; Unilamellar Liposomes

Tibial dyschondroplasia (TD) cases has not been reported in Tibetan chickens (TBCs), but it is commonly seen in commercial broilers characterized by lameness. The underlying mechanism remains unclear. Hypoxia-inducible factors (HIFs) are important regulators of cellular adaptation to hypoxic conditions. In this study, we investigated the role of HIF-1α,
Anoxia ; Blotting, Western ; Chickens ; Growth Plate ; Osteochondrodysplasias ; Poultry ; Reverse Transcriptase Polymerase Chain Reaction ; RNA ; Thiram

The aim of this study was to provide a basis for studying the molecular mechanism of pharmacological action of chlorhexidine digluconate. Fluorescence polarization of n- (9-anthroyloxy) stearic acid was used to examine the effect of chlorhexidine digluconate on differential rotational mobility of different positions of the number of membrane bilayer phospholipid carbon atoms. The six membrane components differed with respect to 2, 3, 6, 9, 12, and 16- (9-anthroyloxy) stearic acid (2-AS, 3-AS, 6-AS, 9-AS, 12-AS and 16-AP) probes, indicating different membrane fluidity. Chlorhexidine digluconate increased the rate of rotational mobility of hydrocarbon interior of the cultured Porphyromonas gingivalis outer membranes (OPG) in a dose-dependent manner, but decreased the mobility of surface region (membrane interface) of the OPG. Disordering or ordering effects of chlorhexidine digluconate on membrane lipids may be responsible for some, but not all of its bacteriostatic and bactericidal actions.
Carbon ; Chlorhexidine* ; Fluorescence Polarization ; Membrane Fluidity ; Membrane Lipids ; Membranes* ; Porphyromonas gingivalis ; Porphyromonas* ; Thiram

Minocycline is a semi-synthetic, broad-spectrum, antimicrobial agent that was first introduced into clinical practice in 1967. Its primary indication is for the treatment of acne vulgaris, where its success has been attributed to a combination of its bacteriostatic and anti-inflammatory activities. There has been recent interest in minocycline use in the treatment of various other chronic inflammatory and immune-mediated diseases such as Behcet's disease and recurrent apthous ulceration. A well-recognized side effect of minocycline treatment is pigmentation, which has been reported in multiple tissues including skin, nail beds, sclera, bone, the thyroid, and teeth. However, pigmentation of the tongue caused by minocycline is very rare. We report four cases of actual pigmented lesions on the tongue and finger nails due to minocycline therapy, followed by a discussion of minocycline-induced hyperpigmentation.
Acne Vulgaris ; Fingers ; Hearing Loss, Sensorineural ; Hyperpigmentation* ; Minocycline ; Pigmentation ; Sclera ; Skin ; Thiram ; Thyroid Gland ; Tongue* ; Tooth ; Ulcer

PURPOSE: Intravesical instillation of bacillus Calmette-Guerin(BCG) is an established and effective therapy for the superficial bladder carcinoma. The viability of BCG is crucial for the induction of a local immune response as well as effective therapy of recurrent superfical bladder carcinoma. Lubricants are used to facilitate catheterization during intravesical instillation of BCG. Moreover bacteriostatic components contained in them have potential to reduce the viability of the BCG. To verify this assumption, inhibitory effect of four commercially available lubricants on the BCG growth was analyzed. MATERIALS AND METHOD: Four different lubricants and their components were co-incubated with Connaught strain BCG and the resultant growth of BCG was assessed. RESULTS: Significant impairment of BCG viability with lubricants was noted. Chlorhexidine digluconate which is the component of lubricant was considered as responsible for this inhibition. CONCLUSIONS: During intravesical BCG, lubricants might reduce the number of viable BCG in clinical use. For this reason, during intravesical immunotherapy with BCG small amounts of lubricants should be used for urethral catheterization and use of lubricant which does not contain bacteriostatic agent should be considered.
Administration, Intravesical ; Bacillus* ; Catheterization ; Catheters ; Chlorhexidine ; Immunotherapy ; Lubricants ; Mycobacterium bovis ; Thiram ; Urinary Bladder ; Urinary Catheterization ; Urinary Catheters