Process of multiply NS4 gene of hepatitis C virus by PCR technique used with cDNA supplied from ARN. Couple of specific preys for NS4 gene and catalytic enzyme was Deep Vent ADN polymerase. Suitable MgSO4 level to multiply NS4 was 4mM. Fasten prey temperature was factor affecting to specificity of product. Result of electrophoresis showed that the most suitable temperature was 55oC and 57OC. In 200 µM dNTPs level, PCN product was sharp and correct to primary predictable size, in 50 or 100 µM dNTPs level, PCR wasn’t viewed, in 150 µM level, PCR was more blear than using 200 µM level. So dNTPs level was 200 µM
Hepacivirus ; Polymerase Chain Reaction ; Genes ; Enzymes

Study on 80 patients from 16 to 55 years old with chronic rhinitis at 108 Military Central Hospital and Central Otorhinolaryngology Hospital and control groups including 40 healthy people from 20 to 50 years old. 60% of these patients had individual allergic history. 61.25% patients have family allergic history. The common diseases were urticaria, allergic eczema, asthma, drug allergy, food allergy. All patients also had 2 or more rhinitis symptoms. The rate of prick test positive at level 1+ was highest. Total IgE level in patients was higher than that in control group. Most of patients had specific IgE level in different limits
Rhinitis ; Diagnosis ; Hypersensitivity

A cross-sectional study on actual situation of using of borax and colour additive in 390 traditional food samples (Lean pork paste, roast pork, fermented pork roll, noodles, green rice flake cake, pyramidal rice dumpling) has been conducted in Ha Tay province during 2002-2003. The results have shown that 95.2% of lean pork paste sample, 79.3% of fermented pork roll, 10.9% of noodles, 60.0% of cake samples were found with borax-an unpermitted chemical in food. Color additive was found in almost examined samples
Food ; Borates ; Epidemiology

Retrospective study included 6894 babies were born at Bach Mai Hospital from 1995 to 1999. Sex ratio was 51.1% boys versus 48.9% girls. Over-term fetal rate tended to increase (5.53%). Rate of cesarean section increased (19.38%). Average weight of infants was 3037 +/- 566g. Risk factors related to abnormalies of reproductive system: rate of congenital malformation was 0.88%, age of parents were under 20 years old at higher risk factor for having premature newborns with congenital malformation and low birth weight than older group. Parents who work in agricultural field have higher risk of having premature newborns with congenital malformation and low birth weight than those work in other fields. Women who had 3 deliveries or more wer more likely to have children with abnomalies than others
Infant, Newborn ; Diagnosis

Health status of 1288 employees working at 9 paint, rubber, paper and and processing wood factories where chemicals were used in production in Ha Noi, Hai Phong and Da Nang was investigated. 93.9% of these workers exposure to chemicals. 72.2% to poison gas. 50% of factories had chemical level higher than allowed standards. 83.3% workers had chemical poison signs in 2 months previous. 54.8% had diseases related to using chemical. Rate of workers had been ill in year 2000 in these factories was 34.4%. Among them, 97.3% exposure directly with chemicals. Chemical-related disease prevalence was 89.6%. 44.4% factories did not hold health examination for employees
Health ; Chemistry

A qualitative study on health workers, pharmacists working at Health Center of Thanh Oai district, Ha Tay province to identify standard time and needs about doctors for this center in 2005. The highest need was that having doctors working at examination department (50%), surgery department (74%), tuberculosis - infectious disease department (81%), pediatric department - emergency and intensive care department (90%). The number of doctors at internal medicine department, traditional medicine department, imaging diagnosis department exceeded the need from 17-142%
Health Facilities ; Time Factors

Study reaction of pancreas in 35 patients (14 males, 21 females, mean age: 49.12) underwent endoscopic retrograde cholangiopancreatogaphy with and without sphinterotomy at Bach Mai Hospital from February 2007 to February 2008. The result showed that: f pure serum amylase increased immediately in 77.14 % patients by fourth hour after surgery. Increasing in pure serum amylase returned by 96 hours after procedure without treatment. Increasing in pure serum amylase depended on some factors as contrast enhanced agents, sphinterotomy or not. Acute pancreatitis was a complication after endoscopic retrograde cholangiopancreatogaphy
Cholangiopancreatography, Endoscopic Retrograde ; Pancreas

A cross-sectional household survey was conducted in February 2005. There were 1.359 households with 91 persons who get sick during last two weeks and 149 mothers who have children less than 5 year of age or have pregnant and 336 householder enrolled in survey. The results indicated that prevalence of diseases was 25.3% (equivalent to 6.7% of the population). Average illness frequency was 1.7times/person/year. Children group under 1 year of age had the highest health care needs (6.2 times/person/year), followed by the elderly group (2.7 times/person/year); children 1-5 years of age (2.5 times/person/year). The use rate of health services was 62.6%. Some factors found to be related to the use of health services were income, age and health care expenditure. Health care services in terms of the availability, convenience, human relations between users and providers should be strengthened in public health facilities. In addition, promote care activities, a proper referral system and strengthening of village health workers should be focused
Health Services ; Family

A cross-sectional survey was carried out on 29 patients with AIDS before death at Dong Da hospital. Two common opportunity infections were tuberculosis (44.8%), Candida infections (10.4%). There was a rather high rate of patient died in condition of fever, diarrhea, weight loss (24.1%) or unknown reason pneumonia (10.4%). Tests indicated several hematological, biochemical and immune disorders: hematocrite level reduced in 100% patients, SGOT increased in 44% patients, SGPT increase in 32% patients, the number of TCD4 reduce under 200 TB/mm3 in 96.6% patients
Acquired Immunodeficiency Syndrome ; Diagnosis ; Death

A study of intervention solutions for the exhaust from 4 thermo-electrical kilns, from synthetic process of in NH3 and dust due to NPK fertilizer production was carried out to evaluate the efficacy of technological improvement solutions in reducing environment pollution. Results: concentration of dust and toxic fumes was reduced, concentration of the exhaust components eliminated to environment through the chimneys of gases-made kilns reduced significantly after changing technology. NH3, CH4, H2 from producing NH3 decreased clearly, dust from producing NPK reduced 1.63-1.92 times
Environmental Pollution ; Gases ; Waste Products ; Fertilizers