87 children aged 7-15 years old were treated in Dong Thap General Hospital from October 1999 to April 2000 were diagnosed as typhoid fever. Patients underwent routine examinations, blood culture and bone marrow culture, and clinical care. Results showed that: Mab-based dot-blot ELISA technique for diagnosing typhoid fever on pediatric patient urine sample was a rapid technique, easy to collect sample, stable sensitivity and specificity, it could be combined with blood culture for early diagnosis of typhoid fever, especially in patients, which underwent treatment. The assay was easy to realized without complicated equipment, and easy to observe the result.
Diagnosis ; Typhoid Fever ; Child

Mentha piperita L.Mentha piperita L. was collected from Sapa, Lao Cai province and cultivated at Co Nhue – Tu Liem – Ha Noi. The content of the essential oil in fresh branches of this peppermint was about 0.16%. Its green yield is 1.85-2.10 Kg/m2. First time in Vietnam, the chemical composition of peppermint oil was analyzed by GC/MS and 43 constituents were identified. The main constituents were L-menthol (47.60%), menthol (24.10%), menthofuran (6.07%) and 1.8 cineol (5.55%), pulegone (4.22%). Base on these data. The quality of Sa Pa peppermint oil is good as one from native origin. Peppermint collected from Sa Pa enrich the gene source of medicinal plant in general and particularly the gene source of essential oil plant group of Vietnam
Oils, Volatile ; Chemistry ; Plants

This paper presented a study on the dynamic of essential oil accumulation in Japanese mint SK33 (M. arrvensis L.) and the influence of storage time on its quantity and quality. This mint was cultivated at Co Nhue experimental station, Tu Liem district, Ha Noi City between 2000 and 2002. The results showed that during the vegetation and development process, the essential oil content in the plant gradually increased and achieved maximum at first branch flowering stage and reduced to minimum at the end of flowering stage. The menthol content in essential oil of Japanese mint SK33 reached the highest value at full flowering stage (81.79%). The best harvest time for this mint is from the beginning flowering stage to full flowering period. After harvesting the whole plants can be stored during 20 days without the change in content and quality of essential oil
oils ; Conservation of Natural Resources ; Oils, Volatile

The definitive diagnosis of nasopharyngeal carcinoma (NPC) depends on the examination of tissue or cells obtained from either the primary site or a metastatic focus. 1120 patients suffering from NPC were reviewed. 880 patients (76.52%) had fine needle aspiration (FNA) in cervical lymph nodes (positive: 84.83%, Se: 95.52%, Sp: 100%, PV(+): 100%, PV(-):57.14%, A:94.37%) 132 patients (11.79%) performed on smears collected from biopsy tissue (positive rate: 63.64%, Se: 84%, SP: 100%, PV(+): 100%, PV(-): 66.67%, A: 87.88%.
Cross Infection ; Drug Resistance

A study on the structure and active mechanism of commission of the health consultation for joining in the army in the River Delta has shown that it was suitable to organize.1 or 2 commissions for each district. This depended on the area of district and number of consultation. An average number of commissioner were 13,47+/- 6,43 in which medical doctor(5,35+/-2,75), assistance doctor(4,25+/-2,56) and nurse (4,02+/-2,72). The intercommune consultation was a suitable way.
Referral and Consultation ; Pharmacology ; Military Personnel

5 flavonoids, named He,Hg (genin of He), A, C, and D were isolated from the peel of Citrus reticu-lata. By comparing with melting points, UV and IR spectra of reference substances, the structure of He, Hg, C and D were shown to be hesperidin, hesperetin, tangeretin and nobiletin
Flavonoids ; Plants, Medicinal ; Medicine, Traditional

This paper presents the study on Japanese mint SK-33 (M.arvensis L.) and its essential oil. After 3 years cultivated and observed this mint at CoNhue - TuLiem-Hanoi, we have some following notes: its essential oil content at full flowering stage is about 0.64-0.71% in fresh aerial part of plants, 1.38-1.55% in fresh leaves and flowers, 5.13-5.29% in absolute dry leaves and flowers. The chemical composition of this oil was analyzed by GC/MS, 32 constituents were identified. The main constituents are menthol (81.79%), menthone (7.86%), isomenthone (4.95%). Japanese mint SK-33 has rather high content of essential oil, good quality and can create a large quantity of underground stems. It is prospective new mint form for cultivation at large area in our country.
Plants, Medicinal ; Oils, Volatile

Berberine and Palmatine were extracted from the powdered Huong Lien pills with a mixture of methanol and hydrochloric acid (100:1) and subjected to HPLC to simultaneously determine the alkaloids. The HPLC technique was carried out on a Lichrosorb RP-8 (10 m, 250x4 mm) column using a UV detector at =345 nm and a mixture of 3,4g potassium dihydrogen phosphate and 1,7g sodium lauryl sulfate dissolved in 1000 ml of water-acetonitrile (1:1) as a mobile phase. The experimental results proved that the proposed HPLC method was rapid, specific, accurate and precise.
Berberine ; Medicine, Traditional ; Chromatography, High Pressure Liquid ; Biochemistry ; Berberine Alkaloids

French spearmint was imported into Vietnam in 1996 and cultivated in Ha Noi. In the first year, it was cultivated by seeds and since the second year it has been planted by stolons. Every year, planting is done in February – March and the crop reaches full blooming in June. The Russian cultivar is also cultivated in Sa Pa (Lao Cai province). Both samples of Mentha spicata essential oil were analyzed by GC/RI, GC/MS and 13C-NMR. In total, 39 compounds have been identified, 28 in the first sample, 31 in the second.
Plants ; Mentha spicata ; chemistry ; oils

Introduction: Successful cryopreservation of spermatozoa must ensure normal newborns after the preservation time. This method frequently can potentially contain cross-infected risks during the cryopreservation process in the liquid nitrogen environment (such as HCV, HIV). A number of researchers reveal that these risks can be eliminated by washing spermatozoa before cryopreservation. However, the problem is whether cryopreservation of washed spermatozoa still retains its morphology and function or not? \r\n', u'Objectives: To evaluate the change of sperm morphology characteristics after which washed sperm cryopreserved from normozoospermia. \r\n', u'Subjects and method: 30 normal semen samples; each sample was divided into two aliquots of washed and unwashed spermatozoa. All samples were cryopreserved in stages of 1, 2 and 30 days. We compared the percentage of spermatozoa with normal morphology before and after which was washed, pre - cryopreservation and post - cryopreservation between the groups. \r\n', u'Results: The percentage 0 spermatozoon with normal morphology after washing was more significantly increased than prior to washing. Post - cryopreservation, this percentage was reduced time by time but acceptable. There is no significant difference between the two ways of preparation before cryopreservation. The percentage of spermatozoa with abnormal head and neck increased significantly after cryopreservation. \r\n', u'Conclusion: The percentage of spermatozoa with normal morphology post - cryopreservation was reduced in both washed sperm and unwashed sperm samples. This percentage was reduced time by time, but there is no difference between the two groups studied. \r\n', u'\r\n', u'
Sperm washing ; Sperm cryopreservation ; Sperm morphology