The sample of faeces of 279 cases at Thanh Luu- Thanh Luong commune, Ha Nam province, especially the children under 15 years old, were collected, in order to identify the rate of intestinal helminthiasis and the effect of mebendazole from October 10-17, 1998. Results: the rate of helminthiasis was 85.3% and all participants were infected only Ascaris lumbricoides and trichirura. The rate of multiinfection was 52.1%. There was no difference of infection by age, sex. Single dose of mebendazole 400 mg eliminated 65% of cases of infection. Vomiting with worm occurred in 1.67% of cases.
Helminthiasis ; Mebendazole

Implementation "the month for the quality of hygiene and safe foods" during 3 years from 2001 to 2003, Ha Nam province gained results at the same time realized some difficulties that should overcome and consolidate. Some evaluations on examined results in "the month for the food hygiene and safety " should contribute to the application of norms for better food safety, as well as contribute to planning, investment and allocation of resources that limit the food poisoning on the areas, protect people health in this province for the coming years
Food Inspection ; Hygiene ; Food ; epidemiology

Background: Nonoperative treatment of blunt splenic injury in children has become routine standardization in Viet Nam. Objectives:This study aims to study the efficacy of nonoperative management (NOM) for blunt splenic injury (BSI) in children. Subjects and method: The records of all the patients treated in National Hospital of Pediatrics with final diagnosis blunt splenic injury between January 2000 and December 2006 were reviewed. The clinical signs, investigations, imaging studies, methods of treatment and results were analyzed. The splenic injuries were graded according to the American Association for the Surgery of Trauma (AAST). Results:There were 15 patients from one day to 12 years of age, with average injury grade 2.2. From 13 patients who were attempted NOM, only 2 patients (blunt liver injury - BLI grade 3 and 4) were operated thereafter because of continuing bleeding or worsening clinically, the remains of 12 recovered well with average hospitalized duration wasf 6 days. The NOM for BLI was successful in 11/13 (84.6%) in our series. Conclusion:NOM was highly efficient method and should be the standard initial approach for all the children with BLI. These patients must be closely monitored for prompt surgical treatment in case of NOM failure.
Spleen/ injuries ; Infant

Background: Nonoperative management (NOM) is presently considered the treatment modality of choice for hemodynamically stable patients sustaining blunt liver trauma, especially in children. Objective: To evaluate role of NOM of blunt liver injuries (BLI) in children at National Hospital of Pediatrics. Subjects and method: Therecords ofallthe patientstreatedin National HospitalofPediatricswithfinaldiagnosisBLI betweenJanuary2000 and December 2006 were reviewed. The clinical signs,investigations,imagingstudies, methods oftreatmentandresultswereanalysed.Theliverinjuriesweregradedaccordingtothe American AsociationfortheSurgeryofTrauma(AAST).Results: There were15patients from one dayto 12 years of age with average BLI grade 2.5; 14 patients with precise diagnosis BLI were atempted NOM, 1 neonate was operated with diagnosis intraabdominal haemorrhage (BLI grade II found intraoperatively). Two patients (BLI grade II and V) from the atempted NOM group were operated thereafter because of hemodynamical instability or continuing bleeding, the remained 12recovered well with average hospital stay of 7.5 days. The rate of succesful NOM for BLI in our series was 12/14 (85.7%). Conclusions: NOM can be applied safely for BLI in children with high successful rate. Patients\ufffd?hemodynamic status may be more important for treatment decision-making than the injury grade according to the AAST based on ultrasound or CT.
Liver/ injuries ; Child ; Role ;

Background: Angiomyolipoma (AML) is a benign renal neoplasm. Patients were often hospitalized in two situations: Renal AML unilateral or AML with Tuberous Sclerosis Complex. Objectives: To discover prospectively the clinical symptoms and evaluate the therapeutic of 5 renal angiomyolipoma unilateral operated in Viet Duc and Huu Nghi hospital from 2004 January to 2006 April. Subject and Methods: The record of 3 men and 2 women with a mean age of 46.2 years (maximum: 86, minimum: 32); flank pain: 3 patients, heamaturia: 2 patients. The mean lesion diameter was 7.9cm (minimum: 5.4 cm, maximum: 12cm). The mean follow up was 6.6 months. Result: All of the patients symptomatic with a mean giant diameter (7.9cm). One patient was operated on urgently, because of a tumor rupture post-traumatism. Two patients were operated on as diagnostic doubt. Conclusion: Renal angiomyolipomas are almost benign. Total nephrectomy on the size of tumor, their complications and the special conditions.
Angiomyolipoma

Background: Urinary bladder sarcomas are rare in adults, accounting for 0.38-0.64% of bladder tumors. The most recognized treatment for malignant tumors is chemotherapy followed by radiotherapy, but there have been no large scale study to provide a definitive regimen for this condition. Objectives: To determine the outcome of patients with urinary bladder sarcomas. Subjects and method: Between January 2002 and December 2006, 6 patients diagnosed with urinary bladder sarcomas were examined at the Viet Duc Hospital. We retrospectively reviewed the records of these patients for information on clinical features, treatment and outcomes. Results: The mean age of patients was 39.6 years; male/female ratio was 3/1; 66% was leiomyosarcomas, 16.7% was rhabdomyosarcomas, 16.7% was myxosarcomas (n=6). Among these malignant tumors, 3/5 patients were in the advanced stage. Conclusion: Bladder sarcomas were rare in adults. Leiomyosarcomas were more common than rhabdomyosarcomas and had worst outcomes if it is in an advanced stage.
Bladder sarcoma ; Surgery

Background: \r\n', u'In the antituberculosis chemotherapy, shortening treatment course, and applying new high effective methods are top prioritized. Of which,the immunotherapy with M vaccea an environmental saprophyte, combined with the antituberculosis medicines all over the world has been applied since 1985, aimed to enhance the host immune responses.\r\n', u'Objectives:\r\n', u'To evaluate the results after 6 months of shortened course anittuberculosis chemotherapy by combining the immunotherapy withM vaccae.\r\n', u'Subjects and method: \r\n', u'233 newly diagnosed pulmonary tuberculosis patients with AFB smear (+) selected from Centers for Tuberculosis Control in some districts of Ha Tay and Ha Noi. They are 15 above, and not the pregnant, breastfeeding, diabetes, HIV infections, and hepatitis B (Unnecessary) \r\n', u'Dividing randomly into 2 groups, group A was in treatment of the standard 8 month chemotherapy regimen, and group B was treated in a shortned 6 month chemotherapy regimen in combination with immunotherapy by using M. vaccae vaccine. \r\n', u'Results:\r\n', u'The rates of AFB (-) sputum of group B were 98,2% and 100% respectively, the rates of the group A were 97,4% and 99% respectively after 2 and 6 months of treatment. There are 88.5% and 100% for group B, 85.6% and 98.8% for group A at the same time to achieve a negative sputum culture for M. tuberculosis. IgM responses against M. tuberculosis sonicated antigen before and after the follow-up time in Group A are not changed, whereas this level of the immunotherapy group had reduced significantly at the 6th moth (P<0.05). There is no responses in IgC to M. tuberculosis of both groups during the same follow-up period. The mean weight of patients in group B was higher than that of group A after 2 and 6 months of treatment.\r\n', u'Conclusion:\r\n', u'M. vaccae vaccine brings good benefits when be combined with the chemo-immunotherapy for newly diagnosed pulmonary tuberculosis patients with AFB smear (+). The negative sputum indicators and clinic improvements in the chemo- immunotherapy group of treatment are better than that of chemotherapy only. This study created more effective method for treating tuberculosis infection.\r\n', u'
Tuberculosis ; Pulmonary ; Tuberculosis ; Mycobacterium tuberculosis ;

Background: Heparan-sufate interacting protetin (HIP) has been known to be up-regulated and expressed in various human cancer cell lines at both transcript and protein levels. HIP\u2019s expression was related to the differentiation status and cancer development. Objective: Using a semi-quantitative PCR method to determine HIP transcript levels in benign prostatic hyperplasia (BPH), prostatic intraepithelial neoplasia (PIN) and prostate cancer tissues. Subjects and methods: 30 samples of BPH, 12 samples of high-grade PIN, and 40 samples of prostate cancer were collected from patients at Viet Duc Hospital and Friendship Hospital. Total RNA was extracted from BPH, PIN and prostate cancer tissues; cDNA synthesis by reverse transcript - polymerase chain reaction (RT - PCR); HIP transcript determination using semi-quantitative PCR. Results: There was significant difference in HIP transcript levels. HIP transcript was very highly up-regulated in the prostate cancer tissues. The up-regulation of HIP transcript was lower in PIN, and lowest in BPH. HIP transcript levels in benign samples were 1/2 and 2/3 compared with cancer and PIN samples, respectively (P< 0.05). These indicated that up-regulation of HIP transcript may be an early event in tumorigenesis. Conclusions: Levels of HIP transcript were different between tissues of prostate cancer, PIN, and BPH. HIP may be a marker for pre-cancer of the prostate.
HIP/L29 ; prostate cancer ; Transcript

Background: Dementia is a common pathological condition that affects older people. Most causes of dementia are Alzheimer\u2019s disease and vascular dementia. Diagnosing these conditions mostly relied on clinical patterns, but some biomarkers have been mentioned as the indicators of this condition. Objectives: 1) To evaluate the alteration of some biomarkers in cerebrospinal fluid (CSF) samples from Alzheimer\u2019s patients. 2) To compare the concentration of biomarkers in CSF samples from patients with vascular dementia and Alzheimer\u2019s disease. Subjects and method: Case group involved 41 patients who were diagnosed as AD and vascular dementia based on DSM-IV criteria. 31 matched healthy people were included in control group. All subjects were given neuro-psychological tests and thorough clinical examination. Brain CT scan and MRI were done for both groups. CSF samples were taken from patients in the study group to measure levels of some biomarkers. Results. The levels of total taurine (T-tau) and phosphorylated taurine (P-tau) 181 proteins are higher in the dementia group. The concentration of Abeta-42 is significantly different between case and control groups, but similar between vascular dementia and Alzheimer\u2019s disease patients. Conclusion: Changes in biomarkers are valuable in different diagnosis of Alzheimer\u2019s disease and other types of dementia. However, findings of CSF studies have to be considered with findings from imaging studies and clinical examination.
Biomarker ; Dementia ; Alzheimer\u2019s disease

Background: The method of immunoelectron microscopy has been found more than 20 years. It is widely applied to detect and identify some types of virus in medical waste samples.\r\n', u'Objectives: To identify antigen location of Rota virus in organelle of the Vero cell and primary monkey kidney cells after infecting and to study the interaction between the virus and host cells.\r\n', u'Subjects and methods: The study was conducted on Rota virus G1P8 (KH0118) isolated from patients with symptoms of acute diarrhea, primary monkey kidney cells collected from Macaca mulatta monkey and the Vero cell of WHO. \r\n', u'Results: Gold particles (10nm) coated protein A and polyclonal antibodies were used to interact directly with Rotavirus proteins \r\n', u'These gold particles with high electron density revealed the antigen location of the Rota virus in the lysosome, pouch and other compartments of the cytoplasm.\r\n', u'Newly assembled viral particles could be identified only after 18-20hours post-infection. It is also noteworthy that viral particles and empty capsides (virus like particles) were comprised into cytoplasmic vesicles associated with the endoplasmic reticulum (ER)-Golgi system.\r\n', u'Conclusion: In order to better understand the interaction mechanism of virus and host cells, the use of this method together with specific monoclonal antibodies for each protein component of viruses and cells is essential.\r\n', u'\r\n', u'\r\n', u'
Rotavirus ; Vero Cells ;