System suitability tests are an integral part of liquid chromatographic technique. They are used to verify the resolution (R) and reproducibility of the analysis to be done. HPLC method for assaying the related substances test of Cefaclor in raw material as well as in pharmaceutical dosage forms introduced to some pharmacopoeia such as EP, USP need to use Delta- 3 cefaclor as the internal standard to verify the suitability of chromatographic system. In case of lacking it to use, Cephalexin can be used as the internal standard in place of Delta-3 cefaclor. cell
Chromatography, High Pressure Liquid ; Cefaclor

Phyllanthoideae is a primitive subfamily of Euphorbiaceae in Vietnam. This subfamily consists of 9 sorts, 17 genera and 192 species. Among which 46 species (23,96%) of the total 192 species used medicinally, most of which are in the Phyllanthus (10 species), Glochidion (9 species), Antidesma (7 species)...Among these, 60.87% are trees, the rest are shrubs and herbs, but no lianas. The most parts for treatment are leaves, and then the roots, the barks, the fruits and the whole trees.
Plants, Medicinal ; Euphorbiaceae ; Biochemistry

Background: Sciatic neuralgia syndrome is a quite common condition in clinical of internal medicine diseases. The syndrome is not life threatening but reduce the ability to work and activities of the patients. The disease is more common in men than women and most of working age.\r\n', u'Objectives: To define the clinical characteristics of sciatic neuralgia syndrome. \r\n', u'Subjects and methods: The cross sectional study was carried out on 48 sciatic neuralgia patients aged from 16 to 70, treated at Bach Mai hospital. The research indices were clinical and traditional medicine characteristics of sciatic neuralgia syndrome.\r\n', u'Results: Sciatic neuralgia was common in the 20-39 year old (87.5%) age group. The male to female ratio was 1.7 (p<0.01). The sciatic neuralgia syndrome rose after excessive exercise (50%). In almost hernia cases of vertebral disc on spinal column was detected by MRI (83.3%). The impaired nerve root was L5 (64.6%). Acupuncture sites were on gallbladder meridian (50.0%), bladder meridian (20.8%) and coordinated (29.2%)\r\n', u'Conclusion: The sciatic neuralgia syndrome causing hernia on spinal column was common at 20-59 year old age. The male number was higher than female. Almost sciatic neuralgia patients had noticed pain on the gallbladder meridian (p<0.05).\r\n', u'\r\n', u'
sciatic neuralgia syndrome ; clinical characteristic

Background: In many studies on aplastic anemic patients, hemorrhage syndrome is expressed in mainly subcutaneous hemorrhage, gum hemorrhage and menorrhagia in women. In traditional medicine, there are many remedies to treat the hemorrhage syndrome in aplastic anemic patients, such as \u201cBao nguyen thang\u201d, \u201cNhi tien thang\u201d, \u201cNhi chi hoan\u201d, \u201cO ke bach Phuong hoan\u201d, \u201cQuy ty thang\u201d herbal remedies\u2026 Objectives: To research the effects of supplemental treatment for hemorrhage syndrome by \u201cDuong quy bo huyet thang\u201d herbal remedy on aplastic anemic patients. Subjects and method: A study was carried out on 60 aplastic anemic patients at Bach Mai Hospital from October 2006 to October 2007. The subjects were divided into two groups: group I was treated with blood transfusion, corticoid and supplemental treatment by \u201cDuong quy bo huyet thang\u201d remedy; group II was only treated with blood transfusion and corticoid. This was a controlled, randomized clinical trial. Results: The average age of the patients was 40.57 +/- 16.77 years in group I and 41.73 +/- 17.14 years in group II (p>0.05). The male-female ratio was 1.07. The number of patients with hemorrhage syndrome decreased from 66.7% to 36.7% after 60 days of treatment. The platelet counts increased from 20.85 +/- 13.57 g/l to 28.94 +/- 16.92 g/l (p<0.05). Conclusions: The \u201cDuong quy bo huyet thang\u201d herbal remedy decreased hemorrhage syndrome in aplastic anemic patients.
\u201cDuong quy bo huyet thang\u201d remedy ; hemorrhage syndrome ; aplastic anemia

Background: Paracheck F.test is rapid diagnostic test that use to determine presence of histidine rich protein 2-HPR-2, a component of Plasmodium falciparum in total blood. Paracheck F.test producted by India\r\n', u'Objective: To study the sensitivity, specificity and diagnosed conformity of Rapid Diagnosis Test (Paracheck F test) compared with microscopic method for detecting P.falciparum \r\n', u'Subject and method: The study was conducted in 5 communes of Quang Tri province and 1 commune of Quang Binh province from September to November in 2006. A total of 470 blood samples were collected and examined with 2 methods for detecting P falciparum (Microscopy and Rapid Diagnosis Test- Paracheck). \r\n', u'Results:Among these blood samples, there were 438 samples having complete conformity in both two methods. 29 samples were negative with microscopy but positive with Paracheck. 3 samples were positive with Paracheck but negative with microscopy.\r\n', u'Conclusion: the sensitivity and specificity of Paracheck is 99% and 82%, respectively. Both microscopic and Paracheck methods have a high conformity in detecting P falciparum with the Kappa index of 0.84. \r\n', u'
Sensitivity ; specificity ; diagnosed conformity ; Paracheck F test ; microscopy

Background: Heparansulfate Interacting Protein (HIP) is up-regulated in various human cancer cell lines at both transcript and protein levels. HIP expression is related to the differentiation status and cancer development. Objectives: To determine HIP in benign prostatic hyperplasia, prostatic intraepithelial neoplasia and prostate cancer tissues. Materials and method: Western blot method was used to determine HIP expression in 3 different types of prostate tissue, including 11 prostate cancer samples, 2 benign prostatic hyperplasia samples and 11 prostatic intraepithelial neoplasia samples. Results. HIP was particularly up-regulated in prostate cancer and prostatic intraepithelial neoplasia, indicating that up-regulation of HIP expression may be an early event in tumorgenesis. Conclusion: The expression of HIP was different between cancer, prostatic intraepithelial neoplasia tissue and benign prostatic hyperplasia. HIP may serve as a prognostic marker for prostate carcinoma.
HIP expression ; Prostate cancer ; Prostatic hyperplasia.

Objective: To evaluate the antitumor activity both in vitro and in vivo of saponin–phospholipid complex of Panax notoginseng. Methods: The in vitro cytotoxic effect of saponins extract and saponin–phospholipid complex against human lung cancer NCI-H460 and breast cancer cell lines BT474 was examined using MTS assay. For in vivo evaluation of antitumor potential, saponin and saponin–phospholipid complex were administered orally in rats induced mammary carcinogenesis by 7,12-dimethylbenz(a)anthracene, for 30 days. Results: Our data showed that saponin–phospholipid complex had stronger anticancer effect compared to saponin extract. The IC50 values of saponin–phospholipid complex and saponin extract for NCI-H460 cell lines were 28.47μg/mL and 47.97μg/mL, respectively and these values for BT474 cells were 53.18μg/mL and 86.24μg/mL, respectively. In vivo experiments, administration of saponin, saponin–phospholipid complex and paclitaxel (positive control) effectively suppressed 7,12-dimethylbenz(a) anthracene-induced breast cancer evidenced by a decrease in tumor volume, the reduction of lipid peroxidation level and increase in the body weight, and elevated the enzymatic antioxidant activities of su-peroxide dismutase, catalase, glutathione peroxidase in rat breast tissue. Conclusions: Our study suggests that saponin extract from Panax notoginseng and saponin–phospholipid complex have potential to prevent cancer, especially breast cancer.

Background: Recently, microplate almar blue assay has been used commonly in detecting gen related to tuberculosis drug resistance, which provides results after 5-7 days with lower cost compared to traditional methods. Objective: To evaluate the application of microplate almar blue assay for rapid detection of drug resistance of M. tuberculosis strains. Subject and Method: A microplate-based assay which uses Alamar blue reagent - an oxidation reduction dye (MABA), was used for the determination of the anti-tuberculosis drug (isoniazid-INH, rifampicine-RIF, streptomycine-STR and ethabuton=EMB) resistance of 96 M. tuberculosis strains isolated from Vietnamese patients in comparison to those obtained by conversional method. Result: MABA showed to have high sensitivity and specificity in testing the sensitivity to individual anti-tuberculosis drugs (from 82.4% for STR to 93.3% for - INH and from 82.5% for EMB to 98.4% for STR; respectively), as well as for the multi-drug resistant M.tuberculosis (86.4% of sensitivity), highly correlated with the result determined by proportion method. Conclusion: MABA reveals the advantage in shortening test time, in simple performance and lower cost compared with the conversional culture based methods.
Microplate almar blue assay ; M. tuberculosis ; drug resistance

Background: With the help of Japan, the Center for Research and Production of vaccines and biologicals, Hanoi has received a WHO standard measles vaccine production technology, including techniques in the examination of vaccine quality. Therefore, it is needed to be initiative on production of measles antibody. Objective: Study on production of measles antibody in rabbits and selecting the appropriate antibody for production of high titre antibody, which meets the standard of vaccine quality control in Vietnam. Subject and methods: Using the measles antigen from Edmonston and AIK-C strains, which were provided by the Kitasato Institute, to produce measles antibody. Making immunoreaction in rabbits and determination of neutralization antibody titre. Results and Conclusion: Measles antigen of Edmonston Vero 7/P2 strain used in the production of measles antibody in rabbit created the highest antibody titre in comparison with AIK-C strain from vero cell and FL cell supplied by the Kitasato Institute of Japan. Antibody titre of Edmonston Vero 7/P2 strain reached up to 1/8192 and met the sera standard required for measles vaccine quality control, it is similar to the measles sera to be produced from the Kitasato Institute.
measles antibody ; antibody titre

Background:\r\n', u'There are two virus known as Nam Dinh Virus, and Colti group B be found in Viet Nam. These viruses have appeared in the South, the Middle and the Highland. They haven\u2019t been reported in the Southern provinces and Can Thoas well. \r\n', u'Objectives: \r\n', u'To identify the circulation of Nam Dinh virus strain, and coltivirus group B strain in Can Tho, Southern Viet Nam, and their existence in nature.\r\n', u'Subjects and method: \r\n', u'Thirty-four mosquito samples (7, 453 individual mosquitoes) from Culex quinque faciatus and Culex pseudovishnui were collected in Can Tho provice, southern Vietnam 2005.\r\n', u'Isolatingviruses on Aedes albopictuc clone C6/36, Vero cells, and using PT- PCR and ELISA Sandwich for identification. \r\n', u'Results:\r\n', u'2 Nam Dinh virus strains, 2 coltivirus group B strains and 1 flavivirus strain (insect flavivirus) were isolated from Culex quinque faciatus, and no virus was isolated from Culex pseudovishnui.\r\n', u'Conclusion: \r\n', u'The identification of the transmission of Nam dinh Virus, and coltivirus group B in Can Tho province by isolating virus from Culex quinque faciatus has shown the evidence for natural vertical transmission of these viruses.\r\n', u'
Viruses ; Coltivirus ; Flavivirus ; Arboviruses ; Culex ;