Yersinia pestis is the cause for the acute infection and may be chosen for biological terrorism. Rapid diagnosis of this agent from infected soil - water is essential. Y. pestis habours 3 specific plasmids providing virulent factors to the bacterium. Objectives: (1) Testing the sensitivity and accurateness of PCR for Y. pestis. (2) Carrying out PCR using total genomic DNA serially diluted as a template. (3) Undertaking PCR on artificical experimentation by diluting Y. pestis in soil water as samples to test PCR based fast diagnostic approach. Methods: Yersinia pestis (inactivated) was used. Genomic DNA was extracted by DNeasy kit (Qiagen Inc). Using primer - pairs PLAF - PLAR (binding on pia gene of plasmid pPCP1) a specific product of PCR was 480 bp. After determination of the PCR sensitivity, a molecular based diagnostic kit was developed. Sensitivity and specificity of this kit was tested by PCR using diluted genomic DNA and bacterium itself; and mix of these templates in water and soil as samples. Results: With the diluted genomic DNA, it was successful to obtain specific PCR with 0.6ng template, which is equal to a single bacterium. Additionally, successful PCR amplification was obtained using the whole bacterium (without extraction of genomic DNA) and diluted quantity ranging from 101 to 102. Based on these results, the bacterium was artificially diluted with sample of soil - water as a natural isolate for PCR amplification. Conclusions: Evidently, approach for PCR-based diagnostic kit was successfully carried out from any template including soil - water samples with high fidelity, using the pia gene genetic marker of pPCR of Y. pestis.
Yersinia ; Water ; Soil

A portion of 652bp of mitochondrial-encoded cob gene was amplified by polymerase chain reaction (PCR) from different Taenia sp samples (tspVN1-10) of different forms of adult, cysticercus isolated in Vietnam. The nucleotide and amino acid sequences of Vietnamese Taenia sp samples were comparatively aligned with the known corresponding sequences of other Taenia species in GenBank. Results showed that TspVN1-3 is Taenia asiatica, TspVn4-6 is Taenia saginata, and TspVN7-10 is Taenia solium. The Vietnamese T.solium is clustered with the Asian T.solium species, while the Vietnamese T.asiatica is clustered with Taiwanese and T.saginata together with the Chinese T.saginata isolate
Taenia ; Parasitic Diseases ; Disease ; Molecular Biology ;

A region of 326 bp of mitochondrial cytochrome oxidase 1 (cox1) for Opisthorchis sp sample collected in An My, Tuy An, Phu Yen, was amplified using PCR. Nucleotide sequence of this cox1 fragment was used to comparatively analyzed with that of O. viverrini, strain Khon Kaen, Thailand, and C. sinensis sequences of Opisthorchis sp of Vietnam, China and Korea. The analysis revealed that nucleotide sequences of Opisthorchis sp of Vietnam has absolute homology to O. viverrini strain Khon Kaen, Thai Lan, but difference from C. sinensis originated from Vietnam, China and Korea. Opisthorchis sp of Vietnam isolated in Phu Yen is, thus, molecularly identified as Opisthorchis viverrini. This gives rise to establish a diagnostic approach and aspect of preventive/control for these species
Opisthorchis ; Molecular Biology ; epidemiology

By molecular identification and using mitochondrial genetic markers, the results showed that different forms of lung fluke in Vietnam, including adult fluke from human, dogs, cats and Potamicus sp. rock crab, have been identified as Paragonimus heteotremus. Molecular-based analysis on 390 nucleotides of the cytochrome oxidase gene revealed that Paragonimus heterotremus of Vietnam from all forms showed high identity to the Chinese and Thai strains. (99.0 -99.2% nucleotide and 97-100% amino acid). Phylogenetic analysis uniquely placed the Vietnamese Paragonimus sp to the group of P.heterotremus of Chinese and Thai origin. Thus, P.heterotremus is offically identified from most of the natural and experimental hosts in Vietnam
Molecular Diagnostic Techniques ; Paragonimus ; Genome, Mitochondrial

Pla sequence composes of 480 nucleodides of pPCP1 plasmide originated from Yersinia pestis isolated from Viet Nam and collected by the technique of PCR of chemical line into TA vector and demonstrated in turn. PCR reaction gave specific result from 3 diversified sources for mould: total extracted ADN, ADN extracted from plasmide isolated from bacterium complete cell. A fast and accurate method of diagnosis was created basing on this operation. By BLAST programme for accessing Gene Bank, the pla-gene sequence of Yersinia pestis in Viet Nam is analogue to other strains worldwide
Yersinia pestis ; Genetic Markers ; Biochemistry

Using polymerase chain reaction (PCR) and sequencing, 492 nucleotides of pra gene was obtained from clinical swabs of the Vietnamese patients for comparative analysis with the corresponding sequences of the global strains of Mecobacterium leprae. As entry data for searching in Genbank, the Vietnamese strains of M.leprae showed very high homology level to the other global strains. In comparision to the standard TN strain, absolute homology (100%) for QH1 (designated as MLVN1) and 98-99% homology for QH2 strain (designated as MLVN2) of the Vietnamese M. leprae was observrd. There may be a number of variants among the M. leprae population in Vietnam. For final conclusion, thus, investigation must be done for many other isolates of patients and geographic origins. This is the first molecular-based identification for M. leprae in Vietnam. Our results afford establishing diagnostic and identification methodologies/techniques for M. leprae from clinical swabs from patients in Vietnam
Mycobacterium leprae ; Molecular Biology ; Polymerase Chain Reaction

The total sequence of 18S rRNA and the neighbours including 1950 pairs of nucleotide (N) was received by PCR and expressed orderly. The examinated sample was 1 of 8 adult worms collected from 12.5 years old male patient. The worm was determined morphologically as Fasciolopsis buski. The comparison showed that in this intestinal fluke worm there is an almost absolutely analogous coefficient concerning nitrogen components of 18S rRNA in only 2 differences on 1950 N (0.01%) with the gene sequence of 18S rRNA kept in the bank of genes. In Viet Nam, this is the first molecular determination realized on human
Fasciolidae ; Trematoda ; RNA, Ribosomal, 18S

Background: Hepatitis C Virus (HCV) is one of thecauses resulting in chronic hepatitis, cirrhosis and primary hepatocarcinoma. In Viet Nam, HCV-infected people are on the increase. The co-existence of HCV and G (HGV) in Viet Nam and their accurate genotyping needs to be clarified. Objective: To detect and molecularly genotype HCV and HGV from 4 serum isolates. Materials: This study consisted of 4 anti-HCV positive [HCV(+)] serum isolates. Method: To extract genomic RNA, perform RT-PCR, 5\u2019UTR fragment of 295 nucleotides for HCV, genomic RNAs from HCV(+) (confirmed by RT-PCR) as samples for RT-PCR for HGV to obtain 260 bp 5\u2019UTR. All of them were cloned and sequenced for analysis. Results: HCV products and HCV + HGV products obtained from samples, respectively; suggested that the co-existence of HCV and HGV could occur in a patient. Base on Gen Back and analysis, we showed that three nucleotide strains of HCV (HCV-H1VN, HCV-H2VN, HCV-H3VN) belongs to genotype 1a of the group 1a/1b, commonly found in East and Southeast Asia. HGV of Viet Nam [HGV-(Han9)VN strain] was identified to belong to type 2; one of five HGV types existing in the world. Conclusions: Three HCV isolates were genotype 1a; one HGV isolate belonged to genotype 2. Detection and genotyping of co-existence of HCV and HGV contributed the development of a multiplex-PCR/RT-PCR for screening blood-transmitted viral hepatitis.
Viral hepatitis ; genotype

Sequences of the complete protein-coding portions of the mitochondrial (mt) genome were analysed for 6 species of cestodes (including hydatid tapeworms and the pork tapeworm) and 5 species of trematodes (blood flukes and liver- and lung-flukes). A near-complete sequence was also available for an additional trematode (the blood fluke Schistosoma malayensis). All of these parasites belong to a large flatworm taxon named the Neodermata. Considerable variation was found in the base composition of the protein-coding genes among these neodermatans. This variation was reflected in statistically-significant differences in numbers of each inferred amino acid between many pairs of species. Both convergence and divergence in nucleotide, and hence amino acid, composition was noted among groups within the Neodermata. Considerable variation in skew (unequal representation of complementary bases on the same strand) was found among the species studied. A pattern is thus emerging of diversity in the mt genome in neodermatans that may cast light on evolution of mt genomes generally.
Amino Acid Sequence ; Animals ; Base Composition ; Base Sequence ; Codon/genetics ; Comparative Study ; DNA, Mitochondrial/*analysis ; Genome ; Molecular Sequence Data ; Platyhelminths/*genetics ; Research Support, Non-U.S. Gov't ; Sequence Alignment ; Species Specificity

Background: People were infected with Cysticercus by either eating Taenia solium eggs from the environment or from proglottids in intestines. These eggs hatched to larvae in muscular and cerebral tissue, which causes specific symptoms. \r\n', u'Objectives: To determine symptoms of human cysticercosis. \r\n', u'Subjects and methods: Clinical and para-clinical processes were conducted on 30 cysticercosis patients, of which 21 were male (70%) and 9 female (30%).\r\n', u'Results and conclusion: The main symptoms were headache in 29 patients (96.7%), subcutaneous cysts in 28 patients (93.3%), epilepsy in 14 patients (46.7%), positive ELISA (antigen of T.solium) with samples in 28 (93.3%) and positive ELISA with CSF samples (73.3%), living cysts in the brain were discovered in 25 patients (83.3%) by CT scanner and eosinophylia in 24 patients (80%). Cysticercus nodules were collected from 28 patients in this study; the species were identified by molecular method. A portion of 652 bp of mitochondrial-encoded cytochrome oxidase b (cob) and 217 amino-acid was amplified by Polymerase Chain Reaction (PCR) and sequenced. The nucleotide sequence was comparatively aligned with the known corresponding sequences of Taenia solium Chinese (TsoCN1). Molecular-based analysis revealed that the Cysticercus from the patients in this study was identified as Taenia solium. There is absolute nucleotide and amino-acid similarity between Taenia solium Chinese (hemogeny 99.1-99.8% of nucleotide and 100% of amino acid).\r\n', u'
Cysticercosis ; diagnosis