1.Protective Effects of Epigallocatechin Gallate After UV Irradiation of Cultured Human Lens Epithelial Cells.
Jun HEO ; Byung Rae LEE ; Jae Woong KOH
Korean Journal of Ophthalmology 2008;22(3):183-186
PURPOSE: To evaluate the protective effects of epigallocatechin gallate (EGCG) against UV irradiation of cultured human lens epithelial cells. METHODS: We irradiated cultured human lens epithelial cells with a 30-second pulse from a UV lamp with an irradiance of 0.6 mW/cm2. Five minutes and 1 hour after UV irradiation, we administered 0, 5, 10, 15, 25, 50, or 100 uM EGCG. The cell number was measured with a microscopic counting chamber and cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. RESULTS: Compared to untreated cells, the total number of cultured human lens epithelial cells was markedly higher after UV irradiation. In a dose-dependent manner, viability was also higher in EGCG-treated cells. CONCLUSIONS: EGCG increased the cell count and cell viability after UV irradiation of cultured human lens epithelial cells, indicating that EGCG can protect lens epithelium against UV damage.
Antioxidants/*pharmacology
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Catechin/*analogs & derivatives/pharmacology
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Cell Count
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Cell Survival/drug effects
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Cells, Cultured
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Coloring Agents/diagnostic use
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Dose-Response Relationship, Drug
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Epithelial Cells/radiation effects
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Humans
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Lens, Crystalline/cytology/*radiation effects
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Radiation Injuries/*prevention & control
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Radiation-Protective Agents/*pharmacology
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Tetrazolium Salts/diagnostic use
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Thiazoles/diagnostic use
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*Ultraviolet Rays