1.In vivo alternative testing with zebrafish in ecotoxicology.
Seung Hyeok SEOK ; Min Won BAEK ; Hui Young LEE ; Dong Jae KIM ; Yi Rang NA ; Kyoung Jin NOH ; Sung Hoon PARK ; Hyun Kyoung LEE ; Byoung Hee LEE ; Jae Hak PARK
Journal of Veterinary Science 2008;9(4):351-357
Although rodents have previously been used in ecotoxicological studies, they are expensive, time-consuming, and are limited by strict legal restrictions. The present study used a zebrafish (Danio rerio) model and generated data that was useful for extrapolating toxicant effects in this system to that of humans. Here we treated embryos of the naive-type as well as a transiently transfected zebrafish liver cell line carrying a plasmid (phAhREEGFP), for comparing toxicity levels with the well-known aryl hydrocarbon receptor (AhR)-binding toxicants: 3,3',4,4',5-pentachlorobiphenyl (PCB126), 2,3,7,8-tetrachlorodibenzo-p-dioxin, and 3-methylcholanthrene. These toxicants induced a concentration-dependent increase in morphological disruption, indicating toxicity at early life-stages. The transient transgenic zebrafish liver cell line was sensitive enough to these toxicants to express the CYP1A1 regulated enhanced green fluorescent protein. The findings of this study demonstrated that the zebrafish in vivo model might allow for extremely rapid and reproducible toxicological profiling of early life-stage embryo development. We have also shown that the transient transgenic zebrafish liver cell line can be used for research on AhR mechanism studies.
Animals
;
Benz(a)Anthracenes/toxicity
;
Cell Line
;
Green Fluorescent Proteins
;
Hepatocytes/cytology/physiology
;
Larva/drug effects/growth & development
;
Lethal Dose 50
;
Polychlorinated Biphenyls/toxicity
;
Tetrachlorodibenzodioxin/toxicity
;
Water Pollutants, Chemical/*adverse effects
;
Zebrafish/*physiology
2.In Utero Exposure to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin Affects the Development of Reproductive System in Mouse.
Mei Hua JIN ; Hae Kyung KO ; Chang Hee HONG ; Sang Won HAN
Yonsei Medical Journal 2008;49(5):843-850
PURPOSE: Exposure of male reproductive organs to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD) has been reported to cause developmental changes. In this study, we evaluated the effects of in utero TCDD exposure on male reproductive development. MATERIALS AND METHODS: Pregnant C57BL/6 mice were administered a single intraperitoneal injection of TCDD (1microgram/kg) on gestation day (GD) 15. The offspring were examined in the immature stage on postnatal day (PND) 30 and in the mature stage on PND 60. The testes were examined for histological changes, androgen receptor (AR), proliferating cell nuclear antigen (PCNA) and apoptosis following the measurement of morphological changes. RESULTS: Anogenital distance (AGD) and testis weights were reduced by TCDD exposure both on PND 30 and PND 60 while body weights and length of male offspring were not affected by TCDD. The regular sperm developmental stage was impaired with TCDD treatment on PND 30. However, no difference was found between the control group and TCDD groups on PND 60. Simultaneously, the expression of AR was also reduced on PND 30, while it was increased on PND 60 compared with the control group. The expression of PCNA was decreased whereas apoptosis was not affected by TCDD both on PND 30 and PND 60. CONCLUSION: These results suggest that in utero exposure to TCDD influences the development of testes by inhibiting the expression of AR and PCNA. Moreover, the adverse effects of TCDD on male offspring reduced over time.
Animals
;
Apoptosis/drug effects
;
Cell Proliferation
;
Embryonic Development/*drug effects
;
Environmental Pollutants/*toxicity
;
Female
;
Male
;
*Maternal Exposure
;
Mice
;
Mice, Inbred C57BL
;
Organ Size/drug effects
;
Pregnancy
;
Receptors, Androgen/metabolism
;
Testis/*drug effects/embryology/pathology
;
Tetrachlorodibenzodioxin/*toxicity
3.Effects of Benzoapyrene, 2-Bromopropane, Phenol and 2,3,7,8-Tetrachlorodibenzo-p-Dioxin on Proinflammatory Cytokines Gene Expression by Mice Spleen Cells.
Ho Jun KIM ; Bit Na KANG ; Sung Whan CHO ; Hwa Young SON ; Kyu Shik JEONG ; Sang Joon PARK ; Sung Ho KIM ; Se Ra KIM ; Tae Hwan KIM ; Mi Young AN ; Si Yun RYU
Journal of Veterinary Science 2002;3(4):247-254
The detrimental effects of environmental pollutants on the health of the individual are generally accepted, although the mechanisms of these effects remain to be incompletely understood. In the present study, we examined the effects of B[a]P, 2-BP, phenol and TCDD on proinflammatory cytokine gene expression in mice spleen cells which were stimulated with anti-CD3. 10-9M TCDD increased IFN gammar and TNF alpha gene expression, but suppressed IL-1 gene expression. 10-6M phenol inhibited IL-1, IL-6 and TNF alpha gene expression, and 10-6M of 2-BP downregulated TNF alpha gene expression. However, 10-6M of B[a]P did not influence on IL-1, IL-6, IFN gammar and TNF alpha gene expression. These findings suggest that TCDD may impair the immune functions of mice by enhancing proinflammatory cytokines production, whereas phenol and 2-BP may impair the functions by inhibiting the production of these cytokines.
Animals
;
Antigens, CD3/immunology
;
Apoptosis/drug effects
;
Benzo(a)pyrene/*toxicity
;
Cells, Cultured
;
Cytokines/*biosynthesis/genetics
;
Environmental Pollutants/*toxicity
;
Gene Expression/drug effects
;
Hydrocarbons, Brominated/*toxicity
;
Male
;
Mice
;
Mice, Inbred C3H
;
Phenol/*toxicity
;
RNA/chemistry/genetics
;
Reverse Transcriptase Polymerase Chain Reaction
;
Spleen/*drug effects/metabolism
;
Tetrachlorodibenzodioxin/*toxicity
4.Teratological effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD): induction of cleft palate in the ddY and C57BL/6 mouse.
Byung Il YOON ; Tohru INOUE ; Toyozo KANEKO
Journal of Veterinary Science 2000;1(2):113-119
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a highly toxic halogenated aromatic hydrocarbon, is a teratogen to induce cleft palate when exposed during the pregnancy. There are inter-strain differences in the sensitivity to cleft palate induced by TCDD and other chemicals including polychlorinated terphenyls (PCTs). The C57BL/6 mouse and the ddY mouse had been shown to be different in the induction of cleft palate following the treatment of PCTs, which attempts us to evaluate the TCDD-induced cleft palate in two mouse strains to understand the mechanism through which TCDD and PCTs induce cleft palate. This study evaluated the induction of cleft palate in the fetuses of ddY and C57BL/6 mice after subcutaneous treatment of TCDD on gestation day (GD) 10.5-14.5 or oral treatment on GD 8.5-13.5. Our results clearly showed that ddY mice, a susceptible strain to PCTs-induced cleft palate, are resistant to the induction of cleft palate by TCDD comparably to the high susceptibility of C57BL/6 mice, suggesting a different teratological mechanism between TCDD and PCTs. In addition, at the low doses, our study supported the concept of "window effect" of TCDD on around GD 12 for the induction of cleft palate in C57BL/6 and ddY mice.
Administration, Oral
;
Animals
;
Cleft Palate/chemically induced/genetics/pathology/*veterinary
;
Female
;
Fetal Diseases/chemically induced/mortality/veterinary
;
Incidence
;
Injections, Subcutaneous
;
Male
;
Mice
;
*Mice, Inbred C57BL
;
Polychloroterphenyl Compounds/toxicity
;
Pregnancy
;
Rodent Diseases/*chemically induced/genetics/pathology
;
Teratogens/*toxicity
;
Tetrachlorodibenzodioxin/administration & dosage/*toxicity
5.Evaluation of biological monitoring markers using genomic and proteomic analysis for automobile emission inspectors and waste incinerating workers exposed to polycyclic aromatic hydrocarbons or 2,3,7,8,-tetracholrodedibenzo-p-dioxins.
Meyoung Kon KIM ; Sangnam OH ; Ji Hye LEE ; Hosub IM ; Yeon Mi RYU ; Eunha OH ; Joohyun LEE ; Eunil LEE ; Donggeun SUL
Experimental & Molecular Medicine 2004;36(5):396-410
In this study, we investigated the effects of PAHs and dioxin on mRNA and plasma protein expression using genomic and proteomic analysis for automobile emission inspectors and waste incineration workers. About 54 workers from automobile emission inspection offices, 31 workers from waste incinerating company and 84 unexposed healthy subjects were enrolled in this study. Urine and air samples were collected and analyzed by HPLC and GC/MS. Comet assays were carried out to evaluate any DNA damage in mononuclear and polynuclear cells. A significant difference in Olive tail moments in mononuclear cells was observed between exposed and control subjects (P <0.0001). To examine the differences of the gene expression profile in automobile emission inspectors and waste incineration workers, radioactive complementary DNA microarrays were used to evaluate changes in the expression of 1,152 total genes. The gene expression profiles showed that 11 genes were up-regulated and 4 genes were down-regulated in waste incinerating workers as compared with controls. Plasma proteins were analyzed by 2-dimentional electrophoresis with pH 3-10 NL IPG Dry strip. The protein expression profiles showed that 8 proteins were up- regulated and 1 protein, haptoglobin, was down- regulated in automobile emission inspectors and waste incineration workers. Serum paraoxonase/ arylesterase was found only in the plasma of waste incineration workers. The expression of genes and proteins involved in oxidative stress were up-regulated in both automobile emission inspectors and waste incineration workers. Several proteins, such as transthyrethin, sarcolectin and haptoglobin, that were highly up- or down-regulated, could serve as biological monitoring markers for future study.
Adult
;
Aged
;
Biological Markers/analysis
;
DNA Fragmentation
;
Environmental Monitoring/*methods
;
Gene Expression Profiling
;
Genetic Markers
;
Genomics
;
Humans
;
*Incineration
;
Middle Aged
;
Naphthols/urine
;
Occupational Exposure/analysis
;
Oligonucleotide Array Sequence Analysis
;
Polycyclic Hydrocarbons, Aromatic/analysis/*toxicity
;
Proteomics
;
Pyrenes/analysis
;
Research Support, Non-U.S. Gov't
;
Tetrachlorodibenzodioxin/analysis/*toxicity
;
*Vehicle Emissions
6.Signaling pathway for 2,3,7,8-tetrachlorodibenzo- p-dioxin-induced TNF-alpha production in differentiated THP-1 human macrophages.
Hyeon Joo CHEON ; Young Seok WOO ; Ji Young LEE ; Hee Sook KIM ; Hyun Jin KIM ; Sungwon CHO ; Nam Hee WON ; Jeongwon SOHN
Experimental & Molecular Medicine 2007;39(4):524-534
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a prototypic halogenated aromatic hydrocarbon (HAH), is known as one of the most potent toxicants. At least a part of its toxic effects appears to be derived from its ability to induce TNF-alpha production. However, the signaling pathway of TCDD that leads to TNF-alpha expression has not been elucidated. In this study, we investigated the signaling mechanism of TCDD-induced TNF-alpha expression in PMA-differentiated THP-1 macrophages. TCDD induced both mRNA and protein expression of TNF-alpha in a dose- and time-dependent manner. Alpha-Naphthoflavone (NF), an aryl hydrocarbon receptor (AhR) inhibitor, prevented the TCDD-induced expression of TNF-alpha at both mRNA and protein levels. Genistein, a protein tyrosine kinase (PTK) inhibitor, and PD153035, an EGFR inhibitor, also blocked the increase of TNF-alpha expression by TCDD, indicating the role of EGFR in TCDD-induced TNF-alpha expression. On the other hand, PP2, a c-Src specific inhibitor, did not affect TCDD-induced TNF-alpha expression. EGFR phosphorylation was detected as early as 5 min after TCDD treatment. TCDD-induced EGFR activation was AhR-dependent since co-treatment with alpha-NF prevented it. ERK was found to be a downstream effector of EGFR activation in the signaling pathway leading to TNF-alpha production after TCDD stimulation. Activation of ERK was observed from 30 min after TCDD treatment. PD98059, an inhibitor of the MEK-ERK pathway, completely prevented the TNF-alpha mRNA and protein expression induced by TCDD, whereas inhibitors of JNK and p38 MAPK had no effect. PD153035, an EGFR inhibitor, as well as alpha-NF significantly reduced ERK phosphorylation, suggesting that ERK activation by TCDD was mediated by both EGFR and AhR. These results indicate that TNF-alpha production by TCDD in differentiated THP-1 macrophages is AhR-dependent and involves activation of EGFR and ERK, but not c-Src, JNK, nor p38 MAPK. A signaling pathway is proposed where TCDD induces sequential activation of AhR, EGFR and ERK, leading to the increased expression of TNF-alpha.
Animals
;
Benzoflavones/pharmacology
;
Cell Differentiation
;
Cell Line, Tumor
;
Enzyme Activation
;
Genistein/pharmacology
;
Hazardous Substances/*toxicity
;
Humans
;
MAP Kinase Signaling System/drug effects/physiology
;
Macrophages/*metabolism
;
Mice
;
Phosphorylation
;
Pyrimidines/pharmacology
;
Quinazolines/pharmacology
;
RNA, Messenger/metabolism
;
Receptor, Epidermal Growth Factor/antagonists & inhibitors/metabolism
;
Receptors, Aryl Hydrocarbon/antagonists & inhibitors
;
Signal Transduction
;
Tetrachlorodibenzodioxin/*toxicity
;
Tumor Necrosis Factor-alpha/*biosynthesis
;
src-Family Kinases/antagonists & inhibitors/metabolism
7.Neurotoxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin in cerebellar granule cells.
Experimental & Molecular Medicine 2005;37(1):58-64
An environmental pollutant, tetrachloro dibenzo dioxin (TCDD) is known to illicit the cognitive disability and motor dysfunction in the developing brain. TCDD induced effects leading to neurodevelopmental and neurobehavioral deficit may have been defined, however underlying molecular mechanism and possible intracellular targets remain to be elucidated. In this study, we attempted to analyze TCDD-induced neurotoxic effects in the granule cells from cerebellum where certain cognitive abilities and motor function command are known to be excuted. [3H]PDBu, (phorbol 12,13-dibutyrate) binding assay indicated that TCDD induced a dose-dependent increase of total PKC activity and its induction was the aryl hydrocarbon receptor (AhR) dependent and N-methyl-D-aspartate receptor (NMDAR) independent. TCDD also caused the translocation of both PKC-alpha and -epsilon in a dose-dependent manner but associated with different receptors; PKC-alpha via AhR but not PKC-epsilon indicating an isozyme-specific pattern of the induction. Increase of the ROS formation was also observed in the cells treated with TCDD in a dose-dependent and an AhR-dependent manner. The treatment of the cells with the diamino dicyano-bis(2-aminophenylthio) butadiene (U0126, MEK-1/2 inhibitor), dizocilpine maleate (MK-801, non-competitive N-methyl-D-aspartate glutamate receptor antagonist) and vitamin E attenuated the TCDD-induced ROS production indicating that TCDD-induced ROS formation may be associated with activation of ERK-1/2 in the MAP kinase pathway or the NMDA receptor. TCDD also increased [Ca2+]i, which is associated with ROS formation and PKC activation in the cerebellar granule cells. It is suggested that TCDD activates the NMDA receptor, which may induce a sustained increase of [Ca2+]i in neurons followed by the ROS formation. Our findings may contribute to understanding the mechanism of TCDD-related neurotoxicity, thereby improving the health risk assessment of neurotoxic compounds in humans.
Animals
;
Binding, Competitive
;
Butadienes/pharmacology
;
Carcinogens/pharmacology
;
Cerebellum/cytology/*drug effects/enzymology
;
Dizocilpine Maleate/pharmacology
;
Environmental Pollutants/*toxicity
;
Enzyme Activation/drug effects
;
Enzyme Inhibitors/pharmacology
;
Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
;
Neuroprotective Agents/pharmacology
;
Nitriles/pharmacology
;
Phorbol 12,13-Dibutyrate/pharmacology
;
Protein Kinase C/*metabolism
;
Protein Transport
;
Rats
;
Rats, Sprague-Dawley
;
Reactive Oxygen Species/metabolism
;
Receptors, Aryl Hydrocarbon/metabolism
;
Receptors, N-Methyl-D-Aspartate/metabolism
;
Research Support, Non-U.S. Gov't
;
Tetrachlorodibenzodioxin/*toxicity