1.Primary carcinoid tumor of the testis: immunohistochemical, ultrastructural and DNA flow cytometric study of two cases.
Hee Jung KIM ; Mee Yon CHO ; Young Nyun PARK ; Jeong Hae KIE
Journal of Korean Medical Science 1999;14(1):57-62
Primary testicular carcinoid tumor, occupying 0.23% of testicular neoplasm, is a rare and indolent neoplasm with the potential for distant metastasis. We present two cases of primary pure carcinoid tumor of the testis. Both patients were 36 years old. Physical examination revealed testicular mass with and without tenderness. The preoperative serum levels of beta-human chorionic gonadotropin and alpha-fetoprotein were normal and neither patient had carcinoid syndrome. The tumors measured 7.5x6x4 cm and 5.5x5x4 cm in size. Histologically, immunohistochemically and ultrastructurally, the tumors showed typical features of the carcinoid tumor. Case 1 showed extensive tumor necrosis and vascular invasion. DNA flow cytometric analysis showed aneuploidy with DNA index of 1.47 and S+G2M of 14.0% in case 1 and tetraploidy with DNA index of 1.96 and S+G2M of 22.1% in case 2. Both patients have been well without any signs of metastasis after operation for 24 months in case 1 and for 16 months in case 2.
Adult
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Carcinoid Tumor/pathology
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Carcinoid Tumor/metabolism
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Carcinoid Tumor/genetics*
;
Case Report
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DNA, Neoplasm/analysis*
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Flow Cytometry/methods*
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Human
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Immunohistochemistry
;
Male
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Testicular Neoplasms/pathology
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Testicular Neoplasms/metabolism
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Testicular Neoplasms/genetics*
2.Primary leydig cell tumor of epididymis: a case report.
Chinese Journal of Pathology 2005;34(4):247-248
Adult
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Biomarkers, Tumor
;
metabolism
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Epididymis
;
metabolism
;
pathology
;
surgery
;
Humans
;
Inhibins
;
metabolism
;
Leydig Cell Tumor
;
metabolism
;
pathology
;
surgery
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Male
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Testicular Neoplasms
;
metabolism
;
pathology
;
surgery
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Vimentin
;
metabolism
3.Large cell carcified Sertoli cell tumor.
Li-Feng WANG ; Shu-Jie ZHANG ; Ji-Ping QI ; Huan-Lin MEI
Chinese Journal of Pathology 2005;34(11):761-762
Calcinosis
;
pathology
;
Child
;
Humans
;
Inhibins
;
metabolism
;
Male
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S100 Proteins
;
metabolism
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Sertoli Cell Tumor
;
metabolism
;
pathology
;
surgery
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Testicular Neoplasms
;
metabolism
;
pathology
;
surgery
;
Vimentin
;
metabolism
4.Mature teratoma of testis with carcinoid component: report of a case.
Chinese Journal of Pathology 2009;38(1):62-62
Carcinoid Tumor
;
metabolism
;
pathology
;
surgery
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Chromogranin A
;
metabolism
;
Humans
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Male
;
Middle Aged
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Orchiectomy
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Teratoma
;
metabolism
;
pathology
;
surgery
;
Testicular Neoplasms
;
metabolism
;
pathology
;
surgery
5.Alveolar rhabdomyosarcoma of left testis: report of a case.
Chinese Journal of Pathology 2012;41(2):128-129
Adenocarcinoma
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metabolism
;
pathology
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Adult
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Desmin
;
metabolism
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Diagnosis, Differential
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Humans
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Lung Neoplasms
;
secondary
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Lymphatic Metastasis
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Male
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Melanoma
;
metabolism
;
pathology
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Rhabdomyosarcoma, Alveolar
;
metabolism
;
pathology
;
surgery
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Sarcoma, Alveolar Soft Part
;
metabolism
;
pathology
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Testicular Neoplasms
;
metabolism
;
pathology
;
surgery
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Testis
;
metabolism
;
pathology
6.Inhibitory effect of connexin43 protein on autophagy in cisplatin-resistant testicular cancer I-10 cells.
Min YUAN ; Shuying DONG ; Yanxue YAO ; Yunzheng MEN ; Kaijin MAO ; Xuhui TONG
Journal of Southern Medical University 2019;39(9):1089-1093
OBJECTIVE:
To investigate the effect of connexin43 (Cx43) protein on autophagy in cisplatin (DDP)-resistant testicular cancer I-10 cells.
METHODS:
The expression of Cx43 proteins in testicular cancer I-10 cells and I-10/DDP cells were detected with Western blotting. I-10/DDP cells were transfected with a full- length mouse Cx43 vector (mCx43) Lipofectamine, the empty vector or Lipofectamine (blank control group), and the changes in the expressions of LC3 and p62 proteins were determined with Western blotting. mCherry-GFP-LC3B transfection and transmission electron microscopy were used to analyze the changes in autophagy of the cells with Cx43 overexpression.
RESULTS:
Cx43 was significantly decreased in I-10/DDP cells compared with I-10 cells ( < 0.01). Transfection of the I-10/DDP cells with mCx43 vector resulted in significantly increased Cx43 expression in the cells ( < 0.01) and caused significantly decreased expression of LC3-Ⅱ ( < 0.01) and increased expression of p62 ( < 0.05) as compared with the negative control cells. Both transmission electron microscopy and mCherry-GFP-LC3B transfection showed that the number of autophagosomes was obviously reduced in mCx43-transfected cells as compared with the negative control cells.
CONCLUSIONS
Cx43 inhibits autophagy in cisplatin-resistant testicular cancer I-10 /DDP cells.
Animals
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Autophagy
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Cell Line, Tumor
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Cisplatin
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Connexin 43
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metabolism
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Drug Resistance, Neoplasm
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Male
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Mice
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Testicular Neoplasms
;
metabolism
;
pathology
7.Expression of a telomerase-associated gene in normal, atrophic, and tumorous testes.
Fang MEI ; Bo ZHANG ; Zhi-Wei TANG ; Lin HOU
Chinese Medical Sciences Journal 2005;20(3):217-220
OBJECTIVETo evaluate the expression of telomerase transcriptional elements-interacting factor (TEIF) in human testis under different status and its relation with human telomerase reverse transcriptase (hTERT) expression.
METHODSSpecific antisera against TEIF were generated by immunization of rabbits with purified recombinated partial TEIF. Samples were assigned to three groups according to their pathological types, including 16 normal testes, 8 atrophic testes, and 6 testicular seminomas. They were subjected to immunohistochemical staining of TEIF and hTERT. Results from both TEIF and hTERT were analyzed semi-quantitatively and compared.
RESULTSThe expressions of TEIF and hTERT were detected in all samples of normal, atrophic testes, and seminomas. No differences of TEIF expressions among these three groups were observed (P > 0.05). On the contrary, the expressions of hTERT were significantly lower in atrophic testes compared with those of normal testes and seminomas (both P < 0.05). Nevertheless, co-expressions of TEIF with hTERT were revealed to be in normal and malignant cases (P < 0.05) but not in atrophic testes, which generally presented TEIF expression. The cellular distributions of both proteins were similar and mainly in spermatocytes and some Sertoli cells, while were all negative in the interstitial cells and other stromal cells. Conclusions The uniform expressions of TEIF in all these specimens suggest that it may be a marker of testis and its related diseases. The strong expression of hTERT in normal testes and testicular seminomas comparing with the low expression in atrophic testes may suggest a role for telomerase in maintaining proliferation of germ cells.
Atrophy ; Biomarkers ; DNA-Binding Proteins ; metabolism ; Gene Expression Regulation, Neoplastic ; Humans ; Male ; Seminoma ; metabolism ; Telomerase ; metabolism ; Testicular Neoplasms ; metabolism ; Testis ; metabolism ; pathology ; Transcription Factors ; metabolism
8.Testicular plasmacytoma: a case report and review of the literature.
Yi-min WANG ; Fang-yin LI ; Jin-dan LUO ; Jun LI ; Li-ping XIE ; Guo-sheng YANG
Chinese Medical Journal 2008;121(10):956-958
CD79 Antigens
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analysis
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Humans
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Immunohistochemistry
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Male
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Middle Aged
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Mucin-1
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analysis
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Plasmacytoma
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metabolism
;
pathology
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Testicular Neoplasms
;
metabolism
;
pathology
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Testis
;
chemistry
;
pathology
9.Pure Choriocarcinoma of Testis with Tumor-Infiltrating Lymphocytes and Granulomas.
Yonsei Medical Journal 2006;47(6):887-891
Pure choriocarcinoma is very rare in the testes, and host immune responses including tumor infiltrating lymphocytes are unusual in choriocarcinoma. This study reports a case of pure testicular choriocarcinoma with extensive lymphocytic infiltrate and granulomatous inflammation. Scrotal ultrasonography revealed a heterogeneous, hyperechoic intratesticular mass. -human chorionic gonadotropin levels were elevated in a radioimmunoassay. The hemorrhagic and necrotic solid mass was composed of two cell populations - mononuclear pleomorphic cells and intimately admixed multinucleated smudged cells. The tumor cells were positive for cytokeratin 7, epidermal growth factor receptors, human placental lactogen and p57. Many inflammatory cells were present within the tumor. The majority of infiltrating cells were CD8-positive cytotoxic cells, which also expressed granzyme-B and TIA-1. The tumor cells were positive for FasL, but negative for Fas. Therefore, this case seemed to escape the host defense response to the tumor due to the loss of Fas, although the cellular host immune response was still active.
Tumor Markers, Biological/analysis
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Testicular Neoplasms/*pathology/ultrasonography
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Male
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Lymphocytes, Tumor-Infiltrating/*pathology
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Humans
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Granuloma/*pathology/ultrasonography
;
Chorionic Gonadotropin, beta Subunit, Human/metabolism
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Choriocarcinoma/*pathology/ultrasonography
;
Adult
10.Pure Choriocarcinoma of Testis with Tumor-Infiltrating Lymphocytes and Granulomas.
Yonsei Medical Journal 2006;47(6):887-891
Pure choriocarcinoma is very rare in the testes, and host immune responses including tumor infiltrating lymphocytes are unusual in choriocarcinoma. This study reports a case of pure testicular choriocarcinoma with extensive lymphocytic infiltrate and granulomatous inflammation. Scrotal ultrasonography revealed a heterogeneous, hyperechoic intratesticular mass. -human chorionic gonadotropin levels were elevated in a radioimmunoassay. The hemorrhagic and necrotic solid mass was composed of two cell populations - mononuclear pleomorphic cells and intimately admixed multinucleated smudged cells. The tumor cells were positive for cytokeratin 7, epidermal growth factor receptors, human placental lactogen and p57. Many inflammatory cells were present within the tumor. The majority of infiltrating cells were CD8-positive cytotoxic cells, which also expressed granzyme-B and TIA-1. The tumor cells were positive for FasL, but negative for Fas. Therefore, this case seemed to escape the host defense response to the tumor due to the loss of Fas, although the cellular host immune response was still active.
Tumor Markers, Biological/analysis
;
Testicular Neoplasms/*pathology/ultrasonography
;
Male
;
Lymphocytes, Tumor-Infiltrating/*pathology
;
Humans
;
Granuloma/*pathology/ultrasonography
;
Chorionic Gonadotropin, beta Subunit, Human/metabolism
;
Choriocarcinoma/*pathology/ultrasonography
;
Adult