No abstract available.
Oncogene Proteins* ; Oncogenes* ; Teratocarcinoma*

OBJECTIVES: Recent basic science studies continue to further our understanding of the fundamental mechanisms that likely underlie the therapeutic benefits of hyaluronan derivatives. The purpose of this study is to elucidate the effects of hyaluronan on ATDC5 proliferation and differentiation. METHODS: ATDC5 cells derived from mouse teratocarcinoma have the capacity to differentiate along a number of connective tissue pathways and are an attractive source of chondrocyte precursor cells. In this study, hyaluronan influencing ATDC5 chondrogenesis were investigated using an bone block culture system. The cell proliferation was analyzed by MTT assay. To validate ATDC5 differentiation we studied ALP activity, collagen content and western blot of Hsp40. RESULTS: In cell proliferation, ATDC5 cells didn't show significant difference between controls and hyaluronan-treated cultures. But hyaluronan induced ALP activity and increased collagen accumulation. Hyaluronantreated ATDC5 cells expressed Hsp40 mRNA and protein within 24 hours. CONCLUSIONS: Hyaluronan-induced chondrogenic differentiation was not associated with ATDC5 cell proliferation. Hyaluronan-induced Hsp40 in cells can protect the cell function from damaged protein. These data provide new insights into regulatory mechanism defining pharmacological effects of hyaluronan.
Animals ; Blotting, Western ; Cell Proliferation ; Chondrocytes* ; Chondrogenesis ; Collagen ; Connective Tissue ; Hyaluronic Acid* ; Mice ; RNA, Messenger ; Teratocarcinoma

Spatially and temporally programmed expression of the Hox genes along the antero-posterior (A-P) axis is essential for correct pattern formation during embryonic development. An accumulating body of evidence indicates the pivotal role of spatial chromatin organization for the coordination of gene regulation. Recently, chromosome conformation capture (3C) technique has been developed and opened a new way to study chromosomal interactions in the nucleus. In this study, we describe 3C method we applied in F9 embryonic teratocarcinoma cells and demonstrate that the chromosomal interactions at Hox loci are successfully detected. Interestingly, at Hoxc loci, the abundance of intrachromosomal interactions with neighboring fragments was drastically decreased when the genes are expressed. These results indicate the possibility of the dynamic pattern of chromosomal interaction in association with the transcriptional regulation of Hox genes.
Axis, Cervical Vertebra ; Chromatin ; Embryonic Development ; Female ; Gene Expression ; Genes, Homeobox ; Pregnancy ; Teratocarcinoma ; Transcriptional Activation

Many drugs can result in a variety of pathologic reactions in the lung, especially the cytotoxic drugs. Amongcytotoxic drugs bleomycin is a prototype. Bleomycin-related pulmonary toxicity is usually known as dose-dependent and can be enhanced with concurrent oxygen therapy, irradiation, or other chemotherapeutic agents. The incidence of bleomycin-induced pulmonary toxicity has been reported as varying from 2 to 46%, and 1% of fatal lung disease. We describe the radiographic and HRCT findings of bleomycin-related pulmonary toxicity developed in two patients: one in ovarian teratocarcinoma, the other malignant lymphoma patient. Chest radiographs and HRCT of these patients showed ground-glass opacities, consolidation, linear and reticular opacities, and interlobular septal thickening. These abnormalities were bilateral, and symmetrical and were found predominantly in the area of mid-and lower-lung zone.
Bleomycin ; Humans ; Incidence ; Lung Diseases ; Lung* ; Lymphoma ; Oxygen ; Radiography, Thoracic ; Teratocarcinoma

The relative rarity and anatomical position of presacral tumors may lead to difficulty in diagnosis and surgical treatment. The clinical features and surgical approach of 4such tumors(teratocarcinoma 1, endodermal sinus tumor 1, chordoma 1, neurilemmoma 1) have therefore reviewed. Low back pain or sacral pain was present in 3patients although all tumors were palpable on rectal examination. Surgical resection was carried out using the posterior transverse approach in Kraske's prone position. Complete surgical excision was performed in 3cases(chordoma, teratocarcinoma, neurilemmoma) and incomplete excision in 1 case(endodermal sinus tumor). A review of literature concerning these tumors and the surgical approach are presented.
Chordoma ; Diagnosis ; Endodermal Sinus Tumor ; Low Back Pain ; Neurilemmoma ; Prone Position ; Teratocarcinoma

Follow-up study was made on 14 patients of testicular who were treated at Severance hospital since 1972. The results are summarized as follows: 1. Among the 14 cases of testicular tumors, there were 7 cases of seminoma and 7 cases of nonseminomatous germ cell tumors(3 cases of embryonal cell carcinoma, 1 case of teratoma, 1 case of teratocarcinoma, 1 case of malignant lymphoma and 1 case of reticulum cell sarcoma.) 2. The median survival periods of 7 patients of seminoma was 35 months(range 6 to 113 months). the median survival periods of 7 patients of nonseminomatous germ cell tumor was 25 months (range 7 to 102 months). 3. The clinical stage of patient was stage A in 9, B in 3 and C in 1, The median survival periods of clinical stage A was 27.3 months, B in 51.3 months and C in 8 months. 4. Preoperative evaluation of serum AFB & HCG was made by radioimmunoassay. Only 1 case of non-seminomatous germ cell tumor had elevated serum AFB, but 2 cases of seminoma & 1 case of non-seminomatous germ cell tumor had elevated serum HCG. 5. The average survival periods according to treatment modalities was 40 months in radiation therapy(6 cases of seminoma), 10 months in chemotherapy only(3 cases of non-seminomatous germ cell tumor), 14 months in RPLND(3 cases of non-seminomatous germ cell tumor) and 16 month in RP LND with chemotherapy(2 cases of nonseminomatous germ cell tumor).
Drug Therapy ; Follow-Up Studies* ; Germ Cells ; Humans ; Lymphoma ; Neoplasms, Germ Cell and Embryonal ; Radioimmunoassay ; Reticulum ; Seminoma ; Teratocarcinoma ; Teratoma ; Testicular Neoplasms

The first case of testicular teratocarcinoma in Korea, found in 24 year old service man was reported. Complication of the metastases to various organs, gynecomastia and hydrocele was seen at the first examination and simple orchiectomy and delayed x-ray deep therapy were not effective to survive this case. The patient died 3 months after the affliction. Autopsy revealed matastases were to lung, spleen, lower poles of both Kidneys, mediastinal and lumbar paraaortic lymph nodes and histopathological examination showed the teratocarcinoma composed of seminoma, embryonic carcinoma and chorioepitheliomatous elements. Additionally, 13 cases of testis tumors reported in literatures of recent years in-country have peen reviewed.
Autopsy ; Gynecomastia ; Humans ; Kidney ; Korea ; Lung ; Lymph Nodes ; Male ; Neoplasm Metastasis ; Orchiectomy ; Seminoma ; Spleen ; Teratocarcinoma* ; Testis* ; Young Adult

A Clinical observation was made of 20 cases of testicular tumor admitted to the Department of Urology, Kei-myung University Medical College and Hospital during the period from May 1971 through July 1982. Among the 20 cases, 4 cases were transferred to this department for further treatment and tumor recurrence following orchiectomy. The results of clinical observation are summarized as below: 1. Age distribution was between 9 months and 57 years old, showing the highest incidence below 10 years old (65%). 2. There were 7 cases of teratoma, 5 cases of seminoma, 5 cases of embryonal carcinoma, 1 case of teratocarcinoma and 2 cases of metastatic neoplasm. 3. Stages of primary germ cell tumors; stage A 6 cases, stage B 2 cases and stage C 3 cases. 4. These patients were managed by surgery, radiation therapy and chemotherapy according to the histologic findings and clinical stages.
Age Distribution ; Carcinoma, Embryonal ; Child ; Drug Therapy ; Humans ; Incidence ; Middle Aged ; Neoplasms, Germ Cell and Embryonal ; Orchiectomy ; Recurrence ; Seminoma ; Teratocarcinoma ; Teratoma ; Urology

Central diabetes insipidus is a rare disorder that can result as a consequence of diverse etiologies, including malformations, autoimmune, infiltrative(e.g. neoplastic or histiocytosis) or traumatic processes, as well as mutations in the gene encoding arginine vasopressin. Idiopathic central diabetes insipidus is a diagnosis of exclusion, one that has been made less frequently through the decades. Idiopathic central diabetes insipidus in children and adolescent requires a frequent follow-up regimen using serial brain MRI and CSF examinations especially if an isolated pituitary stalk thickening or loss of a hyperintense signal in the posterior lobe is observed. Also, so-called "idiopathic" central diabetes insipidus warrants close follow-up to determine the etiology, especially if anterior pituitary hormone deficiencies are detected. We report a case of idiopathic central diabetes insipidus with growth hormone deficiency and loss of a hyperintense signal in the posterior lobe of pituitary in the brain MRI. We followed up with serial contrast enhanced brain MRI and CSF evaluation for the early detection of an evolving occult hypothalamic-stalk lesion and finally detected a newly developed teratocarcinoma in the suprasellar region.
Adolescent ; Arginine Vasopressin ; Brain ; Child ; Diabetes Insipidus, Neurogenic* ; Diagnosis ; Follow-Up Studies ; Growth Hormone ; Humans ; Magnetic Resonance Imaging ; Pituitary Gland ; Pituitary Gland, Posterior ; Teratocarcinoma*

Alpha-FP is a major serum binding protein produced by the fetal yolk sac, liver, and gastrointestinal tract. It may be produced by pure embryonal carcinoma, teratocarcinoma, yolk sac tumor, or combined tumors but not by pure choriocarcinoma or pure seminoma. HCG is secreted by the placenta but in germ cell tumors, syncytiotrophoblastic cells have been found responsible for the production of HCG. Recently, we experienced a case of mixed germ cell tumor composed of mainly teratoma and seminoma which is producing alpha-FP and beta-HCG.
alpha-Fetoproteins ; Carcinoma, Embryonal ; Carrier Proteins ; Choriocarcinoma ; Endodermal Sinus Tumor ; Female ; Gastrointestinal Tract ; Liver ; Neoplasms, Germ Cell and Embryonal ; Placenta ; Pregnancy ; Seminoma* ; Teratocarcinoma ; Teratoma* ; Trophoblasts ; Yolk Sac