ObjectiveTo investigate the influence of recombinant thioredoxin (TRX)on apoptosis of myocardium cell in viral myocarditis of mice.MethodsTwenty-four Balb/c mice,weighting 12 - 14 g,were randomly divided into 3 groups:the control group,the virus group and the protective group,8 mice in each group.The virus group and the protective group were injected with 0.1 ml 100TCID50 Coxackie virus B3 (CVB3)intraperitoneally,and the control group was injected equal volume of saline.Therewithal the protective group was injected with TRX(2 mg/kg) by tail vein,and the virus group was injected saline the same way.After 14 days all mice were killed and hearts were taken.Changes of myocardial histopathology was observed with optical microscope,cell apoptosis was checked by TUNEL technique,and the expression of apoptosis-related proteins (Bcl-2,caspase-3)in infiltrated cell of myocardium was determined by immunohistochemistry.Results(①)Lymphocyte infiltration and necrosis were observed in survivals of the virus group,sporadic coagulation necrosis and ballooning degeneration of cells were observed in the protective group,however no myocardial lesion was found in the control group.(②)TUNEL technique showed that the positive ratio of apoptosis in the virus group and the protective group[(90.23 ± 3.63)％,(20.02 ± 2.41)％] was significantly higher than that of the control group(0.00 ± 0.00,all P ＜ 0.05),the positive ratio of apoptosis in the protective group was significantly lower than that of the virus group (P ＜ 0.05 ).(③)Immunohistochemistry showed that the expression of protein Bcl-2(+,++,+++) in the virus group and the protective group was significantly higher than that of the control group (all P ＜ 0.05).The expression of protein Bcl-2 in the protective group was significantly higher than that of the virus group(P ＜ 0.05).The expression of caspase-3 (+,++) was significantly higher in the virus group and the protective group than the control group (all P ＜ 0.05).Compared with the virus group,the expression of caspase-3 in the protective group was significantly lower(P ＜ 0.05).ConclusionTRX could inhibit cardiomyocyte apoptosis in viral myocarditis mice and the inhibition is related to regulation of apoptosis-related protein expression.

Objective To explore the therapeutic effects of small bone flap craniotomy and traditional craniotomy in patients with hypertensive cerebral hemorrhage. Methods Fifty-six patients with hypertensive cerebral hemorrhage, admitted to our hospital from June 2008 to June 2010, were randomly divided into experimental group (treated with small bone flap craniotomy, n=28) and control group (treated with traditional craniotomy, n=28). The operative time, hospital stays, case fatality rate,re-bleeding and complications and Glasgow outcome scale scores after 6 months treatment were analyzed and compared between the 2 groups. Results Operation time and hospital stays of the patients in the experimental group were significantly shorter than those in the control group (P＜0.05). The case fatality rate was 14.3％ and 25.0％ in the experimental group and control group, respectively, without significant differences (P＜0.05); the incidence of complications was 25.0 ％ and 46.4 ％ in the 2 groups without significant differences (P＜0.05); the scores of Glasgow outcome scale in the experimental group were superior to those in the control group (P＜0.05); the rate of re-bleeding was 10.7 ％ and 14.3 ％ in the 2groups without significant difference (P＞0.05). Conclusion As compared with traditional craniotomy,small bone flap craniotomy can decrease the rate of case fatality and complications, and improve the prognosis in patients with hypertensive cerebral hemorrhage.