1.Detection technique of circulating tumour cells in lung cancer
Fan XIA ; Teng ZHAO ; Yan WANG
Journal of International Oncology 2012;39(7):523-525
Circulating tumor cells(CTCs)are essential for establishing recurrence and metastasis in malignant tumors.Detecting CTCs can help for early detection of the cancer metastases and recurrences,and also can help for evaluating prognostic and guiding treatment.CTCs detection technique mainly include screening and separation technology which contains immune magnetic separation technology and reverse transcriptasepolymerase chain reaction and so on.With the development of technique,there is a new technique named ctcchip contains both screening and separation functions.
2.A comparative epidemiologic survey of hyperthyroidism in areas with different iodine intake
Fan YANG ; Weiping TENG ; Zhongyan SHAN
Chinese Journal of Endocrinology and Metabolism 1986;0(04):-
Objective To investigate the epidemiology of hyperthyroidism in three rural communities with different iodine intake after universal salt iodization (USI) since 1996 as well as the influence of USI on the incidence of hyperthyroidism. Methods Inhabitants in Panshan community did not take iodized salt whereas inhabitants in Zhangwu and Huanghua communities have taken iodized salt since 1996. The thyroid function, thyroid autoantibodies, urinary iodine concentration and thyroid B ultrasound were performed in 1103, 1584 and 1074 subjects respectively in the three communities and the number of hyperthyroidism patients between 1991 and 1999 was collected to calculate the yearly average incidence of hyperthyroidism. Results The median urinary iodine concentrations in Panshan, Zhangwu and Huanghua were 103 ?g/L, 375 ?g/L and 615 ?g/L, respectively (P0.05) andtheprevalenceofsubclinicalhyperthyroidism was 3.7%, 3.9% and 1.1%, respectively. Comparing the yearly average incidence of hyperthyroidism of 1996~1999 with that of 1991~1995, a significant increased incidence in Panshan, while a slight but insignificant elevated one in Zhangwu and a fixed one in Huanghua were found. Conclusion USI can not result in a raise of the prevalence of active hyperthyroidism as well as subclinical hyperthyroidism. The increase of iodine intake is not the only explanation for the raising of the incidence of hyperthyroidism.
3.Comparison of the curative effect of bi-level positive airway pressure and nasal continuous positive airway pressure in the treatment of neonatal respiratory distress syndrome
Yijia FAN ; Guifeng CHEN ; Yiqun TENG
Chinese Journal of Primary Medicine and Pharmacy 2016;23(6):822-825
Objective To compare the clinical application effect of bi -level positive airway pressure ( BiPAP) and nasal continuous positive airway pressure ( CPAP) in the treatment of preterm infants with neonatal respiratory distress syndrome ( NRDS) .Methods 64 neonates with NRDS were divided into the BiPAP group ( n=35) and NCPAP group (n=29) according to the randomized controlled study method.The blood gas index before and after 2,12 hours of treatment,the total time for ventilation,length of stay,and the incidence rate of abdominal disten-sion,gastroesophageal reflux,bronchopulmonary dysplasia,intraventricular hemorrhage,ventilator-associated pneumo-nia in the two groups were compared.Results The levels of pH and PaO2 in the BiPAP group were higher than those in the NCPAP group in noninvasive respiratory support for 2,12h(t=2.391,2.556,2403,2.355,P=0.020,0.013, 0.019,0.022).The incidence rates of hypoxemia,apnea,re-intubation mechanical ventilation rate after 12h in the BiPAP group were lower than those in the NCPAP group(t=5.049,4.988,4.215,P=0.025,0.026,0.040).The length of stay and mechanical ventilation time in the BiPAP group were shorter than those in the NCPAP group( t=-2.096,-2.669,P=0.041,0.010).Conclusion Compared with NCPAP,early application of BiPAP can obviously reduce the invasive respiratory support rate of children with NRDS with intubation.It is worthy of promotion.
4.The evolvement of the action and the application of Astragalus and its development
Ying FAN ; Tie QIAO ; Fei TENG
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(08):-
By sorting out the action and the application of Astragalus in the main works about Chinese herb during the different historical period, the experience that the physician had applied Astragalus could be known, the whole process that Astragalus had been applied from begin has been understood. Based on that study, its beneficial to reasonably apply Astragalus in the clinical application, further to deeply find and succeed the potential and the loosed action and the applied experience of Astragalus. That study provides the theory foundation for further expanding the applied scope of Astragalus, also presents a kind of train of thought for the research and develop of new Chinese herbs.
5.Effects of chronic iodine excess on thyroid function and structure in autoimmune-prone NOD.H-2h4 mice
Xiaochun TENG ; Fan YANG ; Zhongyan SHAN ; Yushu LI ; Chenling FAN ; Weiping TENG
Chinese Journal of Endocrinology and Metabolism 2011;27(4):332-334
To explore the dose- and time- dependent relationship between the chronic iodine excess and thyroid structure, ultrastructure, and thyroid function in autoimmune-prone NOD. H-2h4 mice. Chronic iodine excess leads to iodine-induced goiter with an ultrastructure of follicle epithelial cells injury in a dose and time dependent way.
6.A study on the mechanism of iodine-induced thyroid epithelial cell injury in the induction of autoimmune thyroiditis
Xiaochun TENG ; Na MAN ; Zhongyan SHAN ; Chenling FAN ; Hong WANG ; Rui GUO ; Weiping TENG
Chinese Journal of Internal Medicine 2008;47(3):193-196
Objective To investigate the effect of iodine excess on thyroid follicle epithelial ultrstructure and the relationship between thyroid iniury and autoimmune thyroiditis. Methods NOD.H-2h4 mice and Kunming mice were randomly divided into four groups receiving plain water,5 fold,10 fold,and 100 fold excessive iodine water.4,8 and 24 weeks after receiving iodine water,the mice were killed.After fixation with osmic acid and dual staining with uranyl chloride and citrate lead,thyroid gland ultrstructure was examined with electron microscopy.Resuits Iodine treated NOD.H-2h4 mice exhibited marked accumulation of peroxisome and secondary lysosomes,apoptosis and necrosis of thyroid epithelial cell.damage of thyroid follicles and lymphocvtic infiltration.The observed changes induced by iodine were in a dose dependent way.Conclusion The oxidative iniury on the thyroid epithelial cells induced by iodine excess might be the prerequisite for the creation of autoimmune thyroiditis.
7.Effect of thyrocytes from transgenic mice expressing thyroid specific MHC class Ⅱ on autologous T lymphocyte in vitro
Yushu LI ; Xiaohui YU ; Xiaochun TENG ; Haixia GUAN ; Chenling FAN ; Zhongyan SHAN ; Weiping TENG
Chinese Journal of Endocrinology and Metabolism 2009;25(3):278-279
Thyrocytes expressing MHC class Ⅱ molecules were separated from transgenic mice and were co-cultured with autologous spleen T lymphocytes. T cells did not proliferate and were not activated, but CD4+ T cells were promoted into apoptosis.
8.Discussion on on-line bid of medical equipment
Yue TENG ; Bin ZHANG ; Wei FAN ; Chuanjun LI
Chinese Medical Equipment Journal 1993;0(06):-
Internet-based tendering system makes equipment purchase convenient,rapid,effective,economical and fair. This system guarantees the legal benefits of the medical organizations and suppliers to the most. At the same time this article introduces some issues that should be noticed by medical organizations and suppliers when they participate the on-line bid.
9.Application of medical equipment on-line bid system
Bin ZHANG ; Yue TENG ; Wei FAN ; Chuanjun LI
Chinese Medical Equipment Journal 2004;0(07):-
The medical equipment on-line bid is a newborn method for bid,which unites the modern computer technology,communication technology and electronic commerce technology. When the on-line bid system is applied,except for the make of comprehensive and standard tender documents,the followings also claim attention: how to correctly open the webpage of video meeting inserter,how to suitably adjust video acoustics,and how to properly adjust video images.
10.Analysis of the characteristics of coronary pathological changes and its related factors in patients with impaired glucose tolerance
Yanping TENG ; Jun HE ; Shaojing XI ; Xiaojie WANG ; Qian FAN
Clinical Medicine of China 2011;27(6):578-581
Objective To investigate the characteristics of coronary's pathological changes in patients with impaired glucose tolerance. Methods Four-hundred and ninety patients who were suspected with ischemic chest pain were divided into three groups according to their OGTT results: (1) IGT group: n = 161,(2) 12DM group:n = 159, (3) NGT group: n = 170. Serum levels of triglyceride (TG) , total cholesterol (TC) , highdensity lipoprotein cholesterol (HDL-C) , low-density lipoprotein cholesterol (LDL-C) and high sensitive Creactive protein (hs-CRP) were detected, their body mass indexes (BMI) were calculated. General clinical information (including gender, age, history of smoking, history of hypertension) were collected. All the CAG results were analyzed and Gensini scores were assessed as well. Results The TG levels in the T2DM group and IGT group ([2. 41 ± 1.70] mmol/L and [2. 26 ± 1. 20] mmol/L) were significantly higher than that of the NGT group (1.95 ± 1.14) mmol/L, the differences were significant (t=0.4610,0.3124, P<0. 01 and 0.05,respectively),whereas there was no significant difference between the IGT group and T2DM group (P >0.05);No significant difference was found among the three groups about TC, HDL-C, LDL-C levels (either P > 0.05). The levels of hs-CRP in T2DM group ([2. 38 ± 1. 76] mg/L and IGT group [2. 33 ± 2. 03] mg/L) were higher compared with the NGT group ([1. 54 ± 1. 32] mg/L), the differences were significant (t = 0. 8391,0. 7815, Ps < 0. 01), whereas there was no significant difference between the IGT group and T2 DM group (P >0.05). BMIs of the IGT group ([25.50 ± 3.04]kg/m2) and T2DM group ([26.09 ± 2.86]kg/m2) were higher than that of the NGT group ([24. 70 ± 3. 27] kg/m2), the differences were significant (t = 0. 8063,1. 3947, P<0. 05 and <0.01, respectively),whereas no significant difference was found between the T2DM group and IGT group (P > 0. 05). The incidence of single coronary pathological changes was 44.7% in the NGT group,it was higher than that of the IGT group (23. 6%) and T2DM group (18. 9%) (x2 = 16. 310,25. 116,Ps < 0. 05), whereas there was no significant difference between the IGT group' and T2DM group (P > 0. 05);The incidences of 2 branches pathological changes in the T2DM group (37. 1%) and IGT group (39. 8%) were higher compared with NGT group (23. 5%) ,the differences were significant (x2 =1. 200,10. 099,Ps <0. 05),whereas there was no significant difference between the IGT group and T2DM group (P >0. 05) ;The incidences of 3 vessels pathological changes in the T2DM group (40.9%) and IGT group (33. 5%) were higher than that of the NGT group (20. 0%) , the differences were significant (x2 = 7. 767,17. 028, Ps < 0.05), there was no significant difference between the IGT group and T2DM group (P > 0. 05). The incidence of subtotal or total occlusion of the T2DM group and IGT group were 22. 6% and 18.0% respectively,both were higher than that of the NGT group(7. 6%) (x2 = 14. 573,8. 019 ,Pa < 0.05) , whereas no significant difference was found between the T2DM group and IGT group (P > 0. 05). The incidences of vascular diffusing pathological change in the IGT group (24. 8%) and T2DM group (30. 8%) were higher compared with the NGT group (12.4%) (x2 =8.583,16.724, Ps < 0.05), whereas there was no significant difference between the IGT group and T2DM group (P >0.05). The Gensini scores in the IGT group (55. 05 ± 22. 99) and T2DM group(56. 15 ± 24. 87) were significnatly higher than that of the NGT group (38. 03 ± 17. 38), the differences were significant ((t =17.0142,18. 1186,Ps <0.01),whereas there was no significant difference between the IGT group and T2DM group (P>0.05). Conclusion The incidences of 2 and 3 vessels pathological changes increase significantly in patients with IGT. Moreover, the incidences of occlusion and diffuse stenosis increase significantly. This is similar to the coronary artery pathological charactersitics in patients with diabetes, which indicates that IGT is closely related to the pathological severity of coronary artery. We should pay much attention to those patients with IGT in the clinical work.