Five mongrel dogs were used to investigate the dynamic changes of beta-cndo-rphin-Uke-immunoriactivity(ir-?-EP)and thdr correlation with hemodynamics during intestinal ischemia shock.It was shown that plasma levels of ir-?-EP were increased significantly during shock and were significantly correlated with the decrease in MAP, CO, SV,and CI.Our results give the direct evidence that endogenous opiate beta-endorphin is involved in the cardiovascular pathophysiology of intestinal ischemia shock.

In recent years, promising results have been achieved in retinal pigment epithelium (RPE) cell transplantation in the treatment of diseases such as age-related macular degeneration.The types of transplanted RPE cells include autologous RPE cells, autologous iris pigment epithelium cells, allogeneic fetal RPE cells, allogeneic adult RPE cells, human embryonic stem sell-derived RPE cells, induced pluripotent stem cell-derived RPE cells.According to the transplantation method, RPE cell transplantation can be divided into RPE cell suspension transplantation and RPE cell sheets transplantation.The research on the autologous RPE cells and iris pigment epithelial cells has been reduced due to their limited materials.Fetal RPE cells, which have low immunogenicity and can produce a large number of donor RPE cells, are ideal sources of transplanted cells.Human embryonic stem cell-derived RPE cells and induced pluripotent stem cell-derived RPE cells have adverse effects, including teratogenicity and genetic instability, so transplanted RPE cells need to be optimized.In this article, the experimental researches related to RPE cell transplantation in recent years, the efficacy of transplantation and the existing problems were reviewed.The future trend of RPE cell transplantation may be to establish a comprehensive evaluation system for transplanted cells to screen out relatively suitable donor cells.

Sox2 is one of the important members of the Soxfamily ,It plays an import role in the regulation of early embryo and normal tissue development ,maintain the versatility of stem cells and progenitor cells self -re-newal ability and decide cell fate ,etc.Sox2 genetic mutation or missing may lead to dysplasia or congenital disea-ses.Previous studies showed that Sox 2 expressed in a variety of malignant tumors ,and associated with the inci-dence of malignant tumor ,lymph node metastasis and pathological grading and clinical staging .There fone Sox2 is considered as a potential carcinogenic factor ,its over expression may form one of development of mechanisms for the occurrence and different kinds of malignant tumor .

BACKGROUND:For distal clavicle fracture, clavicular hook plate and Kirschner wire tension band were used in the clinic, but they cannot keep the dual function of stability and functional protection. However, the application of microporous locking plate of distal clavicle makes it possible to double efficacy. OBJECTIVE:To compare and analyze the clinical outcomes of distal clavicle microporous locking plate and clavicular hook plate. METHODS:From March 2011 to March 2013, 40 patients with distal clavicle fractures were involved in this study. They were divided into two groups with the voluntary principles:distal clavicle microporous locking plate group and clavicular hook plate group. We compared and observed perioperative indicators (operation time, blood loss, and hospitalization days), clinical curative effects (University of California at Los Angeles scoring system and American Shoulder and Elbow Surgeons Scale), the score of shoulder joint function (Karlsson standard) and the overal treatment (removed the plate and functional training costs). RESULTS AND CONCLUSION:Al patients successful y underwent surgery. At 1 year after surgery, internal fixators were taken out. Necessary relevant rehabilitation training was performed for more than 3 months. There was no significant difference in perioperative indicators (P>0.05). However, clinical curative effects and the score of Karlsson shoulder joint function showed significant advantages in the microporous locking plate group (P<0.05). Although the distal clavicle microporous locking plate material cost was high, but there was no significant statistic difference in the overal treatment for better protection shoulder function (P>0.05). We draw the conclusion that distal clavicle microporous locking plate can effectively reconstruct the stability of the fracture site, and better retain the function of the joints at the same time.

Thyroid cancer is the most prevalent cancer of endocrine tumors. Its correct diagnosis and differential diagnosis play very important roles in its treatment and prognosis. Ultrasonography, as its powerful tool, is clinically widely used. This article made a review about its main diagnostic methods, including conventional ultrasound, contrast-enhanced ultrasonography and ultrasonic elastography, in order to relfect their panorama.

Objective To analyze syndrome types and TCM prescriptions of Behcet's disease;To summarize the regularity of TCM syndrome differentiation and treatment principles of Behcet's disease.Methods Related literature in CNKI and VIP database from Jan. 1990 to Oct. 2013 was searched. The syndrome patterns, characteristics of prescriptions and frequency of syndrome were summarized.Results There were 20 syndrome types of Behcet's disease in the 58 collected literature. Damp and hot accumulating poison, spleen and kidney Yang deficiency, liver and kidney Yin deficiency, and hot and damp were common syndromes, occupying 55.4%. Hot and damp occupied 41.3%;Yin deficiency occupied 18.1%;Yang deficiency occupied 12.9%. Liver, spleen and kidney were the main disease locations, occupying 94.4%. There were 197 Chinese herbs in total, and could be classified and further combined into 19 types, among which heat-clearing drugs, tonics, activate blood and removing blood stasis were commonly adopted, occupying 50.6%. There were 66 prescriptions collected, among which 31 were set prescriptions, and 35 were prescriptions formed by self-experience. Longdan Xiegan Decoction and Gancao Xiexin Decoction were commonly adopted.Conclusion Syndrome types and TCM prescription characteristics of Behcet's disease had certain regularity, which can provide reference for clinical diagnosis and treatment.

OBJECTIVE:To systematically review the effect of stroke efficacy and bleeding risk of edoxaban versus warfarin in the prevention of patients with atrial fibrillation,and provide evidence-based reference for clinical treatment. METHODS:Re-trieved from Cochrane Library,Medline,EMBase,CJFD,Wangfang Database and VIP Database,randomized controlled trials (RCT)about the stroke efficacy and bleeding risk of edoxaban versus warfarin in patients with atrial fibrillation were collected. Me-ta-analysis was performed for the incidences of stoke and excessive hemorrhage by using Rev Man 5.3 software after data extract. RESULTS:Totally 13 RCTs were included,involving 24 950 patients. Results of Meta-analysis showed,compared with warfarin group,there were no significant differences in the incidences of stoke [RR=0.97,95%CI(0.88,1.08),P=0.60] and excessive hem-orrhage [RR=0.84,95%CI(0.59,1.19),P=0.33] in edoxaban group. But the subgroup analysis showed,when daily dose of edoxa-ban was more than 30 mg,the incidence of stroke in edoxaban group was significantly lower than warfarin group [RR=0.84,95%CI(0.72,0.97),P=0.02];when it was 30 mg,the incidence of excessive bleeding in edoxaban group was significantly lower than warfarin group [RR=0.46,95%CI(0.35,0.61),P<0.001],the difference was statistically significant. CONCLUSIONS:Compared with warfarin,higher doses(>30 mg/d)of edoxaban can more effectively prevent the occurrence of stroke and low doses(30 mg/d) can reduce the risk of excessive bleeding.

Objective To study the levels of soluble endoglin (sEng) in peripheral blood and intervillous space blood of patients with preeclampsia, and analyse the correlation between levels of sEng and clinical performance. Methods Levels of sEng in peripheral blood and intervillous space blood of 22 patients with preeclampsia (preeclampsia group, 12 cases of severe preeclampsia and 10 cases of mild preeclampsia) were detected by ELISA, and the correlation between levels of sEng and blood pressure, 24 h urine protein and fetal birth weight was analysed. Twenty-two normal pregnant women were served as control group. Results The sEng levels in peripheral blood and intervillous space blood of preeclampsia group were significantly higher than those of control group [ (31.89 ± 8.80) ng/mL vs (5.24 ± 1.60) ng/mL, P < 0.01; ( 37.74 ± 7.12) ng/mL vs (6.63 ±1.76) ng/mL, P < 0.01]. In preeclampsia group, the sEng levels in peripheral blood and intervillous space blood of severe preeclampsia were significantly higher than those of mild preeclampsia (P <0.01). In preeclampsia group, the sEng level in peripheral blood was significantly correlated with that in intervillous space blood of preeclampsia group (r = 0.876, P < 0.01) , neither was significantly correlated with blood pressure (P > 0.05), both were significantly correlated with 24 h urine protein (r = 0.729, P < 0.01; r = 0.743, P < 0.01) , and both were significantly correlated with fetal birth weight (r = - 0.736, P < 0.01; r = - 0.707, P < 0.01). Conclusion Levels of sEng in peripheral blood and intervillous space blood of patients with preeclampsia are significantly higher than those of normal pregnant women, and the levels of sEng are significantly correlated with the clinical performance of preeclampsia.

Unilateral thyroidectomy (lobeetomy) plus (or) isthmectomy were performed surgically and all patients recovered satisfactorily. No recurrence was found after a mean of 37 months follow-up(9 months-6 years).