1. Role of MAPK signaling on human mast cell activation induced by Trichomonas vaginalis
Giimaa N ; Arim MIN ; Myeong Heon Shin ; Gurbadam A ; Tsogtsaikhan S ; Temuulen D
Innovation 2016;10(1):38-42
Trichomonas vaginalis is a flagellated protozoan parasite that causes vaginitis and cervicitis in women and asymptomatic urethritis and prostatitis in men. Mast cells have been reported to be predominant in the vaginal smears and vaginal walls of patients infected with T. vaginalis. Mitogen activated protein kinase (MAPK) activated by various stimuli also regulate the transcriptional activity of various cytokine genes in the mast cells. Because of their essential role in intracellular signaling network, also appropriate targets for pharmacological treatment of inflammatory disorders.Cultivation of T.vaginalis and HMC-1 line, preparation of TvSP, to check intracellular ROS level and degranulation by FACS, to determine phosphorylation of MAPK and p47phox by immunobloting.In this study, we investigated whether MAPK were involved ROS generation and exocytotic degranulation in HMC-1 induced by T. vaginalis-derived secretory products (TvSP). We first examined that TvSP could induce activation of MAPK and NADPH oxidase in HMC-1 cells. Stimulation with TvSP induced phosphorylation of MAPK and p47phox in HMC-1 cells. Phosphorylation of p47phox is main source of ROS generation. Next, to determine involvement activation of MAPK in ROS generation and degranulation in HMC-1 cells induced by TvSP. ROS generation is required for exocytotic degranulation of mast cells induced by TvSP. Stimulation with TvSP induced phosphorylation of p47phox, ROSgeneration, and surface up-regulation of CD63 in human mast cells. CD63 is a marker for exocytosis. Pretreatment with MAPK inhibitors strongly inhibited TvSP-induced ROS generation and exocytotic degranulation.Our results suggest that TvSP could induce intracellular ROS generation and exocytotic degranulation in HMC-1 via MAPK signaling pathway.
2.The detection of trichomonas vaginalis by cytological stained tests
Bayarmaa E ; Bayarhishig CH ; Enkhzul B ; Giimaa N ; Temuulen D ; Kyung Il Im
Mongolian Medical Sciences 2012;161(3):8-10
Background: One of the confronted problems of doctors and medical personnels is sexually transmitted disease has not been decreased in our country up to now. By last 5 years propagation of trichomoniasis was 16.7-9.5% per 10000 population and infected T.vaginalis. In the practice of parasitology of our country metilen is revealed by gram method hasn’t been introduced which is used in up to date histological analyse. This became the background of our research work. Aim of our research is to diagnose trichomoniasis which infects sexually by cytological analyse and to define its specific and sensibility.Materials and Methods: A total 99 smears of 33 females aged 19-39, used cross sectional descriptive method. Finding T.vaginalis on specimens (1) Vaginal wet mount, (2) Gram staining and (3) Pap stain. Result: In our research T.vaginalis leaked out in wet mount smear was 33%, in Gram stain was (21.2%). The sensibility quality of T.vaginalis on Gram stain is 63%, specific quality is 90%, value of kappa coefficient (К=0.58 Р<0.002). In Pap stain T.vaginalis diagnosed 27,2% and sensibility quality of T.vaginalis is 81%, specific quality is 100%, value of kappa coefficient (К=0.87 Р<0.005). In the case when 3 analysis were positive case was 5 or 15.2%, in the case where 2 analysis were positive case was 6 or 18.2%. Conclusions: The Pap stain sensibility quality of T.vaginalis is 81%, specific quality is 100%, value of kappa coefficient (К=0.87 Р<0.005), that shows should be to give effect on diagnosing of sexually transmitted infectious disease.
3.ЭХЭМҮТ-Н ХЗП-Н СОНСГОЛ ЗАСЛЫН КАБИНЕТААР ҮЙЛЧЛҮҮЛСЭН ГАДНА ЧИХНИЙ ГАЖ ХӨГЖИЛТЭЙ ХҮҮХДҮҮДИЙН ХЯНАЛТЫН СУДАЛГААНЫ ЗАРИМ ҮР ДҮН
Ariuntuya D ; Zolzaya Ts ; Enkhtunsag B ; Temuulen B
Innovation 2018;12(3):18-21
BACKGROUND: Microtia is often associated with hearing loss and patients typically
require treatment for hearing impairment and surgical ear reconstruction. The
occurrence of microtia is of public health importance in part due to the psychosocial
sequelae, including the stigma associated with malformations of the ear and the burden
of undergoing multiple surgeries
In addition, greater than 90% of individuals with microtia experience conductive hearing
loss on the affected side. Although children with microtia-anotia are at a greater risk of
delayed language development and attention deficit disorders.
METHODS: Our study has a 173 childrens from the UB city and countryside. 23 children
has a bilateral microtia and anotia. 6 patient wearing BAHA soft band from 6 months
age. 150 patients have an unilateral microtia. Microtia was found more commonly in
males, unilateral and right sided. Even children with bilateral microtia may have wearing
BAHA soft band before 6 months age.
RESULTS: External ear malformations are more commonly found in males. Sex ratio 2:1.
From other studies right ear malformations are more common in male children. In our
study children who have bilateral ear abnormalities wearing BAHA softband and
language therapy from 6 months age to able to have normal language development.
Children who had surgical treatment and using hearing aid can have normal social life
and increased quality of life.
CONCLUSION: Early screening in children who have external ear malformation,
monitoring language development, wearing BAHA softband from 2 months age who
have bilateral ear malformation and one ear affected other one is normal children
wear BAHA softband increases children speech and language development.