No abstract available.
Arthritis, Rheumatoid* ; Osteoarthritis ; Telomerase*

Currently, the telomerase is being considered as a marker of cancer because 90% of cancer cells in human have positive telomerase. The telomarase used to early diagnose, monitor and predict after the treatment. In addition to, telomerase also is objective of many researches to treat the cancer. It had better combine the anti telomerase and chemotherapy
Urologic Neoplasms ; Telomerase

Telomerase is a ribonucleoprotein enzyme. Which is responsible for catalyst for increasing chains of telomere and limitation of shortening the chromosome terminal head. Telomerase has activity for the primary cells but inhibited differentiate cells. Telomerase was considered most significantly as a marker of cancer because 90% of cancer cells in human had positive telomerase. The telomerase was used to early diagnose and monitor as well as predict outcome. The antitelomerase therapy was a proper method to prevent relapsed cancer after the traditional treatments
Urologic Neoplasms ; Telomerase

No abstract available.
Breast Neoplasms* ; Breast* ; Telomerase*

PURPOSE: A sensitive predictive assay is necessary to determine the total radiation dose according to sensitivity of individual cancer cell lines. This study is performed to determine whether the radiation sensitivity is correlated with the changes in telomerase activity after irradiation. MATERIALS AND METHODS: Two colorectal cancer cell lines with different radiation sensitivity were used. In order to confirm the difference in radiation sensitivity, we used a calorimetric assay. Telomerase activities were measured using the PCR-based telomeric repeat amplification protocol (TRAP). RESULTS: We confirmed the difference in radiation sensitivity between NCI-H630 and NCI-H716. Survival fractions at 2 Gy were 0.836 for NCI-H630 and 0.317 for NCI-H716. Telomerase activity increased after irradiation with NCI-H630, which was more resistant to radiation, whereas telomerase activity decreased with NCI-H730. But dose-dependent change of telomerase activity was not confirmed. CONCLUSION: Our results suggested that telomerase activity change after irradiation could be used as a predictive assay for radiation response. Further studies with different cell lines and tumor tissues are necessary.
Cell Line ; Colorectal Neoplasms ; Radiation Tolerance ; Telomerase*

BACKGROUND: Telomeres are simple repeats elements located at each end of the chromosomes of eukaryotic cells. The main function of telomeres is to cap the chromosome end and protect it from enzymatic attack. Telomerase that facilitates the synthesis of telomere has been detected in not only cancer, but also in precancerous lesion. In this study, we compared the telomerase expression between low-grade and high-grade gastric dysplasia. METHODS: The telomerase expression of 43 patients with gastric dysplasia (22 low-grade and 21 high-grade) was evaluated by immunohistochemical staining in tissues. RESULTS: The telomerase expression was much higher in the tissues from the patients with high-grade gastric dysplasia than in those tissues of the patients with low-grade gastric dysplasia. CONCLUSIONS: Activation of telomerase may be related with the malignant potentiality in gastric cells. Further studies are needed to define the role of telomerase in gastric tumorigenesis.
Carcinogenesis ; Eukaryotic Cells ; Humans ; Immunohistochemistry ; Telomerase* ; Telomere

Humans ; Metabolic Syndrome ; Telomerase ; metabolism ; Telomere

The unique ability of tumour cells to proliferate indefinitely is crucial to neoplastic progression as it allows these cells to express the aggressive properties of cancer without the censure of physiological ageing. This is in contrast to normal somatic cells which are subject to a "mitotic clock," a phenomenon that has been linked to telomeric shortening after each round of cell replication, so that eventually the loss of genetic material reaches a critical stage and the cells undergo senescence and cell death. A study was conducted to investigate the role of telomerase, an RNA-containing enzyme that restores the telomere length, in the neoplastic cell immortalization and progression process. Fresh human tissue samples taken from excision specimens received by the Department of Pathology, University of Malaya Medical Centre, were investigated for telomerase activity using a commercial Telomerase PCR-ELISA kit (Boehringer Mannheim). Specimens comprised 33 breast lesions (10 infiltrating breast adenocarcinoma, 13 fibroadenoma and 10 non-neoplastic breast tissue), 27 colonic lesions (17 colonic adenocarcinoma and 10 non-neoplastic colonic mucosa) and 42 cervical lesions (20 cervical carcinoma and 22 non-neoplastic cervical tissues). Telomerase activity was found in 6 (60%) of 10 breast carcinomas, 6 (46%) of 13 fibroadenomas, none of the 10 nonneoplastic breast samples, 3 (17.6%) of 17 colon carcinomas and none of the 10 non-neoplastic colonic mucosal samples, 12 (60%) of 20 cervical carcinoma and 3 (13.6%) of 22 non-neoplastic cervical samples. 5/10 (50%) Stage I, 4/7 (57%) Stage II, 2/2 (100%) Stage III and 1/1 (100%) Stage IV cervical carcinomas showed telomerase activity. These findings support a contributory role for telomerase in tumourigenesis with activation occurring from neoplastic transformation and increasing with tumour progression.
Telomerase ; seconds ; Breast ; neoplastic cell ; Progression

No abstract available.
Animals ; Kidney* ; Proliferating Cell Nuclear Antigen* ; Rats* ; Telomerase*

PURPOSE: Telomerase is an enzyme that immortalizes cells by maintaining a constant telomere length, and is thought to be active in the transformation of normal somatic cells into immortal human tumor cells. In an attempt to get a more valuable, noninvasive assay for the detection of bladder carcinomas, exfoliated cells from the voided urine of patients for the presence of telomerase were assayed. MATERIALS AND METHODS: Voided urine samples were obtained from 37 patients with known, but untreated, bladder carcinomas and 20 healthy volunteers to determine the presence of telomerase activity. Telomerase activity was analyzed using a PCR-based telomeric repeat amplification protocol (TRAP)-ELISA method. RESULTS: Of the 37 bladder carcinoma samples, 30 (81.1%) and 16 (43.2%) tested positive for the presence of telomerase activity and cytology, respectively (p=0.009). However, for the 20 healthy volunteers samples, no telomerase activity was found. 83.3% (5 of 6) of the grade 1 tumors, 75.0% (18 of 24) of the grade 2 tumors and 100% (7 of 7) of the grade 3 tumors were positive for telomerase activity. Only 33.3% (2 of 6) of the grade 1 tumors, 45.8% (11 of 24) of the grade 2 tumors and 42.9% (3 of 7) of the grade 3 tumors were diagnosed by cytology. For the low (Tis, Ta, T1) and high (T2, T3) stages, the sensitivities were 77.8 and 100, and 37.0 and 60.0% for telomerase activity and cytology, respectively. All of T1 and T2 patients, with carcinoma in situ, and Ta (100%) were also positive for telomerase activity. There was no significant correlation between the urinary telomerase activity and clinicopathological characteristics of bladder carcinomas, with the exception of the multiplicity of the tumor. CONCLUSIONS: These findings suggest that urinary telomerase activity may be a potential marker for detecting bladder carcinomas, especially in low grade or stage tumors.
Carcinoma in Situ ; Healthy Volunteers ; Humans* ; Telomerase* ; Telomere ; Urinary Bladder*