1.The Effects of Epigallocatechin on Adipogenesis of 3T3-L1 Preadipocytes.
Seong Geun PARK ; Jun Sik KIM ; Nam Gyun KIM ; Tea Gyu PARK ; Jung Young KIM
Journal of the Korean Society of Plastic and Reconstructive Surgeons 2004;31(1):83-88
Preadipocyte cell lines are useful models for investigating adipogenesis process. 3T3-L1 preadipocyte, which can be induced to differentiate into adipocyte in cell culture, is one of the most studied preadipocyte cell lines. When exposed to the appropriate differentiation inducer, including insulin, dexamethasone and 3-isobutyl- 1-methylxanthine, 3T3-L1 preadipocytes differentiate into adipocytes. This study was undertaken to evaluate the effect of epigallocatechin on differentiation of 3T3-L1 preadipocyte, and to test whether epigallocatechin can be useful to reduce fat deposition. Cell proliferation was determined by MTT(3,4,5-Dimethylthiazol-2,5-Diphenyl- Tetrazoliumbromide) spectro-photometry, fat content followed by cell differentiation was determined by Oil Red O staining, and the degree of differentiation into adipocytes were determined by measuring mRNA content and activity of glyceraldehydes 3-phosphate dehydrogenase (G3PDH). Epigallocatechin inhibited proliferation of preadipocytes, not into adipocytes. Fat storage during 3T3-L1 preadipocyte conversion into adipocyte was inhibited by epigallocathechin. Epigallocatechin decreased G3PDH, leptin mRNA and peroxisome proliferator-activated receptor(PPAR)gamma mRNA which were increased by differentiation of preadipocyte into adipocyte. These results suggest that epigallocatechin has a potential to serve as a fat-reducing drug.
Adipocytes
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Adipogenesis*
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Cell Culture Techniques
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Cell Differentiation
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Cell Line
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Cell Proliferation
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Dexamethasone
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Insulin
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Leptin
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Oxidoreductases
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Peroxisomes
;
RNA, Messenger
2.Immunohistochemical characteristics of Monocyte chemotactic protein-1 in the Endometrium of women with Endometriosis.
Dong Ho KIM ; Tea Chul KIM ; Gyu Hyun KANG ; Min HUR ; Eun Sub PARK ; Dae Won KIM ; Doo Suk CHOI ; Jung Goo KIM
Korean Journal of Obstetrics and Gynecology 2000;43(12):2140-2145
No abstract available.
Chemokine CCL2*
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Endometriosis*
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Endometrium*
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Female
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Humans
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Monocytes*
3.Clinical Significance of Measuring Levels of CEA, CA19-9 in Peritoneal Washing Fluid in Patients with Gastric Cancer.
Gyu Beom SHIM ; Ji Hun PARK ; Tea Young KOO ; Hyun Sik MIN
Journal of the Korean Gastric Cancer Association 2006;6(3):125-131
PURPOSE: Free cancer cells exfoliated from cancer-invaded serosa contribute to peritoneal dissemination, the most frequent pattern of recurrence in patients with gastric cancer. To detect free cancer cells, CEA and CA19-9 were introduced as the markers of gastric cancer, and many methods, such as cytology, immunoassay, and reverse transcription polymerase chain reaction (RT-PCR), exist for detecting them. The aim of this study is to define the clinical significance of using immunoassay to measure the levels of CEA and CA19-9 in the peritoneal washings in patients with gastric cancer. MATERIALS AND METHODS: The peritoneal washing fluids were obtained from 130 patients with gastric cancer who received a curative gastrectomy, palliative gastrectomy or open and closure. The pCEA and pCA19-9 levels were measured by using immunoassay and cytology. The results were compared with the clinicopathological data. RESULTS: The pCEA and pCA19-9 levels were correlated with tumor invasion, lymph-node metastasis, and stage (P<0.05). CONCLUSION: A correlation was found between elevated pCEA and pCA19-9 levels measured by immunoassay and the TNM stage. Therefore, a combined pCEA and pCA19-9 assay could be a sensitive detector of peritoneal dissemination, as well as a predictor of postoperative prognosis. pCEA and pCA19-9 may also determine the adjuvant management strategy.
Gastrectomy
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Humans
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Immunoassay
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Neoplasm Metastasis
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Polymerase Chain Reaction
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Prognosis
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Recurrence
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Reverse Transcription
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Serous Membrane
;
Stomach Neoplasms*