To investigate the role of human pepillomavirus(HPV) infection on DNA content of cervical cancers, thirty-seven cases af ceryical squamous carcinoma were studied with the method of flow cytometry for deoxyribonucleic acid(DMA) content and Southern blot hybridizalion for typing of HPV type 1.6 or type 18 DWA in cornbination with other elinical pnrameters. There were 18 dipleid cases(48.6%) and 19 aneuploid(51.4%). The mean age of the patients with diplaid and aneuploid tumars was 51.6 years and 53.6 years, respectively, No significant age difference was found between diploid and aneuploid groups. Six of 13(46.2%) carcinnma in eiiu and 13 of 24(54.2%) invasive cervical carcinoma were aneuploid, and t,he proportinn of aneuploid tumors was not significantly different between the two groups. Six of 10 tumors(60.0%) in stage I, 5 of 11 tumors(45.5%) in stage II, 2 of 3 tumors(66.7%) in stage III were aneuploid. The frequency was not significantly different according to the clinical stage, Aneuploidy was present in 50.0% of HPV 16 positive lesion, 66.7% of HPV 18 positive lesion, and 41.7% of HPV 16 and 18 negative lesion. There was no statistically difference in the incidenee of aneuploidy when all HPV lesions were compared. Of 25 patients with HFV 16 or l8 positive lesion, aneuploidy was demonstrated in the specimen of four of eight patients with careinoma in situ and nine of 17 with invasive cervical carcinoma. In suromary, we found thst the ineidere of DNA aneuploidy was somewhat higher when HPV 18 positive lesion was compared to HPV 16 and 18 negative lesion but the difference was not statistically significant.
Aneuploidy ; Blotting, Southern ; Carcinoma, Squamous Cell ; Diploidy ; DNA* ; Flow Cytometry ; Human papillomavirus 16 ; Human papillomavirus 18 ; Humans ; Humans*

Thirty-nine cases of invasive cervical cancer with human papillomavirus(HPV) DNA sequences were analyzed to detennine if HPV type 16 or 18 clinical or prognostic significance. HPV type was determined by Southern blotl hybridization. HPV 16 was detected in 12 cases, and HPV 18 in 5 cases. Sixty percent of HPV 18 tumors were grade III (3 of 5), whereas 8.3% (1 of 12) of HPV 16 tumors and 5.6% (1 of 18) of HPV 16/18 negative tumors were grade III. Age, clinieal stage, histologic cell type, lesion size, and liyrriph node metestasis in relation to HPV type were not statistically significant. The mean age of HPV 1~6 group was 50 years, compared to 47 years for the HPV 18 group. Of 30 squamous carcinomas, HPV 16 was detected in 12 cases(40.0%), and HPV 18 in 4 cases (13.3%). Of 4 adenosquamous and adenocarcinomas, HPV 16 was detected in 0 case(0.0%), and HPV 18 in 1 case(25.0%). Among stge IB-IIA caners, lymph node metastasis was associated with 20% of HP-V 16 cases(2 of 10) as cotinparxl with 25% of HPV 18 cases(1 of 4) and 7.7% Of HPV 16/18 negative cases (1 of 13). It is suggested that HPV type 18 might be associated with worse prognostic factos of invasive cervical cancer than HPV type 16.
Adenocarcinoma ; Base Sequence ; Carcinoma, Squamous Cell ; Human papillomavirus 16 ; Human papillomavirus 18 ; Humans* ; Lymph Nodes ; Neoplasm Metastasis ; Uterine Cervical Neoplasms*

No abstract available.
Hysterectomy* ; Urinary Bladder*

Locally advanced cancer of the uterine cervix is a major cause of death worldwide. Standard treatment with rdiolherepy for locally advanced cancer of the uterine cnvix has a response rate of less than 50%. Resently concurrent chcmoradirothcrpay has been introduced but with some contvovesy. Interferon and retinoic acid are inducible proteins which posses many hiologic activities such as, antiproliferative, immunomodulatory and antineoplastic properties. Combination of interferon and retinoic acid has produced high response rates especially for patients with squamous cell carcinoma . And they may potentiate the radiation cytotoxicity as adiosensitizer. This study was underaken to assess the clinical efficacy of combination regimen with interferon-alpha2a, 13-cis-retinoic acid and radiotherapy from Dec. 1988 to Dex. 1994 at Severance hospital Yonsei uniersity. Twenty seven patients of locally advanced squamous cell carcionma of the uterine cervix enrolled in this study are evaluated for response and toxicity. The results were as follow: 1. Preliminary results of interferon-alpha2a 13-cis-retinoic acid and radiotherapy are 46.7% of response rate(33.3% complete response)and those of concurrent chemoradiotherapy are 41.7% of response rate(46.7% complete response). 2. Major toxicity of interferon-alpha2a, 13-cis-retinoic acid and radiotherapy is fever(60.0%), and only case of grade 2 anemia and one case of grade 1 AST/ALT elevation was noted. There was no grade 3 or 4 toxicity. Systemic interferon-alpha2a, 13-cis-retinoc acid and radiotherapy is highly active, well tolerated therapy for locally advanced cervical cancer.
Anemia ; Carcinoma, Squamous Cell* ; Cause of Death ; Cervix Uteri* ; Chemoradiotherapy ; Female ; Humans ; Interferons ; Isotretinoin* ; Radiotherapy* ; Tretinoin ; Uterine Cervical Neoplasms

Locally advanced cancer of the uterine cervix is a major cause of death worldwide. Standard treatment with rdiolherepy for locally advanced cancer of the uterine cnvix has a response rate of less than 50%. Resently concurrent chcmoradirothcrpay has been introduced but with some contvovesy. Interferon and retinoic acid are inducible proteins which posses many hiologic activities such as, antiproliferative, immunomodulatory and antineoplastic properties. Combination of interferon and retinoic acid has produced high response rates especially for patients with squamous cell carcinoma . And they may potentiate the radiation cytotoxicity as adiosensitizer. This study was underaken to assess the clinical efficacy of combination regimen with interferon-alpha2a, 13-cis-retinoic acid and radiotherapy from Dec. 1988 to Dex. 1994 at Severance hospital Yonsei uniersity. Twenty seven patients of locally advanced squamous cell carcionma of the uterine cervix enrolled in this study are evaluated for response and toxicity. The results were as follow: 1. Preliminary results of interferon-alpha2a 13-cis-retinoic acid and radiotherapy are 46.7% of response rate(33.3% complete response)and those of concurrent chemoradiotherapy are 41.7% of response rate(46.7% complete response). 2. Major toxicity of interferon-alpha2a, 13-cis-retinoic acid and radiotherapy is fever(60.0%), and only case of grade 2 anemia and one case of grade 1 AST/ALT elevation was noted. There was no grade 3 or 4 toxicity. Systemic interferon-alpha2a, 13-cis-retinoc acid and radiotherapy is highly active, well tolerated therapy for locally advanced cervical cancer.
Anemia ; Carcinoma, Squamous Cell* ; Cause of Death ; Cervix Uteri* ; Chemoradiotherapy ; Female ; Humans ; Interferons ; Isotretinoin* ; Radiotherapy* ; Tretinoin ; Uterine Cervical Neoplasms

No abstract available.
Cervix Uteri* ; Female ; Lymph Nodes* ; Neoplasm Metastasis* ; Risk Factors*

No abstract available.
Humans ; Humans*

No abstract available.
Humans ; Laparotomy* ; Ovarian Neoplasms*

No abstract available.
Female ; Fibroma* ; Ovary* ; Teratoma*

Proliferating cell nuclear ntigen (FCNA) iis a nuclear protein that is syntheaimd in late Gl and S phases of cell cycle and is correlated with the cell proliferative stale. The recent study demonstrated that FCNA functions in 13NA replication. The present study evaluated proliferetive iindices (PI) for the assessment of tumor proliferation and for investigating prognostic significancx, in cervical tumors. lmmunohiatoehemical PCNA staining was perfurmed in formalin-fixed paraffin-embedded cervical tissues via the avidin-biotin-complex immunoperoxidase methad. Mean PI was 36.03+/-5.14% in normaI controls, as compared to 66.19+/-11.36% in cerviml intraepithelial neoplasia. and 63.19+/-10.94% in invasive cervical cancer. Our results showed no significant correlation between Pll and histological type. Among invasive cervical cancer (24 cases), PI waa 64.43+/-10.94% in squamoua cell carcinoma and 59.00+/-4.10% in adenocarcinoma. There was no eipiifiant relationship between Fl and clinical etage, and between PI and lesion size. This study auggeste that Pl may not serve as a new prognostie factor in cervical tumors.
Adenocarcinoma ; Cell Cycle ; Nuclear Proteins ; Proliferating Cell Nuclear Antigen* ; S Phase ; Uterine Cervical Neoplasms